proteins amino acids and enzymes xi nitrate reduction
Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... The high levels of glutamine and glutamate (nitrogen-rich ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc ... ()61TTATTT)56 and )37TTATTT)32), GLU2 [)506TTATTT)501 and )90TTATTT)85 ()strand)], GLT ()311TATAAAT)305), carA ()277TATATAA)271 and )188TTATTT)183), and carB [)361TTATTT)356 and )143TTATTT)138 ()strand)] ... the biosynthesis and transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological...
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barrett - amino acids and peptides (cambridge, 2004)
... ‘Non-protein amino acids , alias ‘non-proteinogenic amino acids or ‘non-coded amino acids 1.9 Coded amino acids, non-natural amino acids and peptides in nutrition and food science and in human ... page xiii Foreword Introduction 1.1 Sources and roles of amino acids and peptides 1.2 Definitions 1.3 ‘Protein amino acids , alias ‘the coded amino acids 1.4 Nomenclature for ‘the protein amino acids , ... geological and extra-terrestrial distribution of amino acids 1.11 Amino acids in archaeology and in forensic science 1.12 Roles for amino acids in chemistry and in the life sciences 1.12.1 Amino acids...
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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
... responses [38] Since whey protein hydrolyate containes approximately 13.4% amino acids as BCAA and arginine [17], we selected 0.1 g amino acids/ kg/hr in this study A limitation of this study is that ... The peak and average power in the matches was similar in the trials After the supplementation, CHO and CHO+AA trial showed significantly higher glucose and insulin, and lower glycerol and non-esterified ... effect of branched-chain amino acids and arginine on glucose disposal and performance was also investigated Nine well-trained male wrestlers participated in trials in a random order Each trial...
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amino acids, peptides and proteins in organic chemistry
... another by the number and sequence of the constituent amino acids Generally, a molecule comprised of few amino acids is called an oligopeptide and that with many amino acids is a polypeptide ... Medicinal Chemistry of a-Hydroxy-b -Amino Acids 189 Zyta Ziora, Mariusz Skwarczynski, and Yoshiaki Kiso Introduction 189 a-Hydroxy-b -Amino Acids 189 a-Hydroxy-b -Amino Acids Occurring in Natural Products ... of proteins is genetically controlled A protein molecule is synthesized by stepwise linking of unprotected amino acids through the cellular machinery comprised of enzymes and nucleic acids, and...
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Tài liệu Proteins và Amino Acids trong thức ăn thủy sản pptx
... nghèo lượng) ng) Enzymes (các proteins hồng cầu ảnh hưởng điều ng nh ng hòa biến dưỡng) Plasma proteins (các proteins di động thể ng miễn dòch thể ) Hormones (vai trò điều hoà) Proteins thành phần ... tính chất nhiệt hay acid hóa ng Proteins liên kết Nucleoproteins: protein + nucleic acid (e.g., seed germs) glycoproteins: protein + COH group (e.g., mucus) phosphoproteins: protein+ hợp chất chứa ... động cân protein sinh tổng hợp tiêu hao protein cho lượng chức sinh học khác Oxy hóa amino acids Các amino acids bò khữ gốc NH3 phản ứng (oxy hóa) hay chuyển gốc amin Khữ gốc NH3 phương thức chuyển...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... of the fulllength proteins Recombinant SUR2A and SUR2B hydrolyzed MgATP slowly, with maximal turnover 2656 rates of 6.1 · 10)3 s)1 and 2.3 · 10)3 s)1, and Km values of 373 and 38 lm, respectively ... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino ... [8] This may also reflect construct differences: our NBD1 is 31 amino acids longer at the N-terminus, and our NBD2A is 26 amino acids shorter at the N-terminus, than those of Park et al [8] To...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... base without amino acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids ... specific amino acids in bacterial EFG [26–29] To date, there are only a few reports in which this technique has been used to acquire information on the importance of specific amino acids and amino ... expressing mutants T56M and T56M ⁄ T58W responded by a reduction in the growth rate by approximately 35% and 45%, respectively (Fig 3C,D) The fraction of dead cells was increased to approximately the same...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... tail (amino acids 1–18), the helical transmembrane region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and ... Vibrio aminopeptidase and the alignment of partial amino acid sequences from human GCPII, human transferrin receptor, yeast aminopeptidase Y, S griseus aminopeptidase, and Caenorhabditis elegans ... DNA sequence encoding the GCPII variant (amino acids 44–750) in the pMTNAEXST plasmid was excised by digestion with BglII and XhoI restriction enzymes and ligated into BamHI/XhoI sites in a pcDNA4/HisA...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... several amino acids may be essential for hepatocellular taurocholate uptake because mutations in these amino acids caused lack of transport; the amino acids in question are Asp115, Glu257, and Cys266 ... Turku, Finland) Results Search for conserved negatively charged amino acids, cysteines and C-terminal threonines by sequence identity An alignment of the amino acid sequence of nine SBAT proteins, ... blocks of amino acids Mutated amino acids are highlighted in gray The resulting mutants are shown in boxes, with deletions indicated (del) Fig Mutations of negatively charged conserved amino acids...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction capacity All alanine substitutions of amino acids ... positions 15 and 37, the putative a-helix is flanked by proline residues Within the helix, two candidate FxxFF protein interaction motifs (F is any hydrophobic amino acid residue and x is any amino acid ... The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction between androgen...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates...
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Báo cáo khoa học: Endoplasmic reticulum stress caused by aggregate-prone proteins containing homopolymeric amino acids doc
... hydrophobic 30-residue HPAAs, and the longer HPAAs (homopolymeric Ala70 and Gln150) as model proteins for polyalanine and polyglutamine diseases The intracellular localization and western blotting of ... hydrophobic HPAAs Aggregate-prone proteins, including disease-unrelated proteins, are thought to have common toxic mechanisms such as increasing intracellular Ca2+ and causing oxidative stress [26] A similar ... HPAAs, based on their cytotoxicity Homopolymeric Ile and Leu inhibited proteasome activity and caused excess ER stress Homopolymeric Ile, Leu and Val are the strongest cytotoxic HPAAs among 20 tested...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... Expression and purification of wild-type and mutant enzymes Full-length and truncated CpcE and CpcF proteins were expressed with N-terminal His- and S-tags using the pET-30a vector The proteins ... full-length cpcF, P5 and P2 for cpcE(42–276), P1 and P6 for cpcE(1–272), P1 and P7 for cpcE(1–274), P1 and P8 for cpcE(1–237), P9 and P4 for cpcF(21–213), P10 and P4 for cpcF(10–213), P3 and P11 for cpcF(1–160), ... showed more homology: a 10 amino acid truncation reduced the activity to 26%, and it was lost completely when 20 amino acids were truncated Among the 10 N-terminal amino acids, I9 is highly conserved...
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Báo cáo lâm nghiệp: "Influence of flooding on growth, nitrogen availability in soil, and nitrate reduction of young oak seedlings (Quercus robur L.)" docx
... whether soil nitrate depletion could affect the absorption and reduction of nitrate in roots and leaves and the amino acid partitioning in Quercus robur seedlings under soil hypoxia stored in ... at days 0, 26 and 34, n = in control roots at days 26 and 54 (*) and (**) indicate significant differences between flooded and control at p < 0.05 and p < 0.01 respectively; (Mann and Whitney test) ... This could influence N-turnover in soil and thereafter nitrogen assimilation pathways in roots 4.3 Nitrate reduction Nitrate reduction can occur both in roots and shoots but the relative contribution...
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Báo cáo y học: " Albumin dialysis improves hepatic encephalopathy and decreases circulating phenolic aromatic amino acids in patients with alcoholic hepatitis and severe liver failure" doc
... tests, arterial ammonia levels, and the concentration of total amino acids, branched amino acids, phenolic aromatic amino acids and tryptophan by high-performance liquid chromatography Briefly, ... total, branched and aromatic amino acids Results are shown in patients with alcoholic hepatitis (AH) and pruritus (control) Circulating levels of total, branched and aromatic amino acids before ... • Total amino acid and phenolic aromatic amino acids diminished and the Fischer ratio increased in patients with alcoholic hepatitis treated with MARS • Changes in amino acid levels and the Fischer...
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Báo cáo y học: "A computational method to predict genetically encoded rare amino acids in proteins" pdf
... selenoproteins (for example, thioredoxin, hesB); glycine reductase genes (GRD); and new candidate selenoproteins (c) A section of the multiple sequence alignments (MSA) of the newly predicted candidate ... selenoproteins include SelD, GrdA and GrdB (from the anaerobic glycine reduction pathway), HesB (associated with the nitrogen fixation genes), and several oxidoreductases (for example, thioredoxin ... example, thioredoxin and peroxiredoxin) In archaea, selenocysteine usage appears to be confined to a small group of enzymes in the anaerobic methanogenesis pathway [23] (such as FDH and formylmethanofuran...
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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation
... primary ligand can exhibit biological activity (Noorulla and Sreenivasulu, 2011; Singh et al., 2010; Freeman, 1973; Che and Siu, 2010; Podunavac-Kuzmanovic and Cvetkovic, 2007) and an amino acid ... synthesis, characterization and antibacterial activities of synthesized iron(III) and copper(II) complexes of mixed ligands, 8-hydroxyquinoline and alanine or methionine amino acids: [M(HQ)(AA)nH2O, ... Iron(III) and copper(II) complexes bearing 8-quinolinol with amino- acids mixed ligands 745 0.5 χmT / cm3Kmol -1 0.4 0.3 0.2 0.1 0.0 20 40 60 80 100 T/K Figure IR spectra of the ligands and complexes...
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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients
... study on the roles of amino acid neurotransmitters and nitric oxide in chronic and acute pain Analysis of pain-related amino acids including the nitric oxide markers – citrulline and arginine in CSF ... physiologic amino acids in cerebrospinal fluid without pretreatment Clin Chem 2004;50:665-9 Sethuraman R, Lee TL, Chiu JW, Tachibana S An analysis of excitatory and inhibitory amino acids and nitric oxide ... using HPLC for amino acids in CSF was developed, which was then subsequently applied to quantitatively analyze all physiological amino acids especially the nine pain-related amino acids (asparagine,...
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Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions
... substances Phase uptake Toxin Phase I enzyme Toxin [O] Phase II enzyme Toxin-OH III enzyme excretion Toxin-conjugate ATP ADP Pi Figure 1.1 Drug detoxification Phases I, II, and III (Adapted from ... growth and viability To gain insight into the role of key amino acid residues of MRP4 protein for transport organic anions and resistance to chemotherapeutic compounds, three aromatic amino acids ... domains and important amino acid residues for MRP4 function 45 1.7.1 Substrate binding domains .45 1.7.2 Identification of the key amino acids .47 1.8 Resistance to purine and...
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