proteins amino acids and enzymes vi coagulase and dnase activity
Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... The high levels of glutamine and glutamate (nitrogen-rich ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc ... the GLT mutant in high CO2 (Table 1) The GDH activity varied between 75% and 135% of the wild-type activity for glutamate synthesis and between 45% and 65% for glutamate oxidation in the three ... nitrogen via the tracheary elements for amino acid synthesis and subsequent export of the derived amino acids via phloem sieve elements for use by sink cells (Fig 8) Cellular localization of GOGATs and...
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barrett - amino acids and peptides (cambridge, 2004)
... Sources and roles of amino acids and peptides 1.2 Definitions 1.3 ‘Protein amino acids , alias ‘the coded amino acids 1.4 Nomenclature for ‘the protein amino acids , alias ‘the coded amino acids ... Sources and roles of amino acids and peptides More than 700 amino acids have been discovered in Nature and most of them are ␣ -amino acids Bacteria, fungi and algae and other plants provide nearly ... for amino acids in chemistry and in the life sciences 1.12.1 Amino acids in chemistry 1.12.2 Amino acids in the life sciences 1.13 - and higher amino acids 1.14 References Conformations of amino...
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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
... anched-chain amino acids prolong exercise dur ing heat stress in men and women Med Sci Sports Exerc 1998, 30:83-91 61 Davis JM, Welsh RS, De Volve KL, Alderson NA: Effects of br anched-chain 33 amino acids ... The peak and average power in the matches was similar in the trials After the supplementation, CHO and CHO+AA trial showed significantly higher glucose and insulin, and lower glycerol and non-esterified ... the performance and (2) whether BCAA and arginine could provide additive effect on glucose disposal during the recovery and the performance in the subsequent match Mater ial and methods Subjects...
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amino acids, peptides and proteins in organic chemistry
... another by the number and sequence of the constituent amino acids Generally, a molecule comprised of few amino acids is called an oligopeptide and that with many amino acids is a polypeptide ... Medicinal Chemistry of a-Hydroxy-b -Amino Acids 189 Zyta Ziora, Mariusz Skwarczynski, and Yoshiaki Kiso Introduction 189 a-Hydroxy-b -Amino Acids 189 a-Hydroxy-b -Amino Acids Occurring in Natural Products ... (molecular weight below 10 000) Proteins contain a large number of amino acids Due to the vitality of their role for the function as well as survival of cells, peptides and proteins are continuously...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... suggests that the final 42 amino acids of SUR2B may reduce its hydrolytic activity, and that the catalytic activity of SUR2A is not measurably affected by its final 42 amino acids In contrast to what ... NBD2B (NBD2 of SUR2B), and NBD2-DC ATP hydrolysis by NBDs NBD1 and NBD2A displayed higher ATPase activity than NBD2B (Fig 2A; Table 1), with NBD1 having ATPase activity of SUR2A and SUR2B A MBP–SUR2 ... is 31 amino acids longer at the N-terminus, and our NBD2A is 26 amino acids shorter at the N-terminus, than those of Park et al [8] To our knowledge, this is the first time that the activity of...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... study This study Invitrogen A Invitrogen Invitrogen Invitrogen Invitrogen This study B Fig Galactose induced expression levels and ribosome association of plasmid-encoded mutant and wild-type eEF2 ... specific amino acids in bacterial EFG [26–29] To date, there are only a few reports in which this technique has been used to acquire information on the importance of specific amino acids and amino ... Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids used are listed in Table Plasmid pFA6a-HIS3MX6 was kindly provided by C...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... denaturing SDS buffer and loaded onto a single lane Activity levels are indicated as follows: (+), measurable NAAG-hydrolyzing activity; (+/–), extremely low activity; (–), no activity; ND, not determined ... buffer and 10 lL of the mixture was loaded onto a single lane Activity levels are indicated as follows: (+), measurable NAAG-hydrolyzing activity; (+/–), extremely low activity; (–), no activity; ... hydrolytic activity [15] The protein is proposed to consist of six domains: the N-terminal cytoplasmic tail (amino acids 1–18), the helical transmembrane region (amino acids 19–43), and four extracellular...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... several amino acids may be essential for hepatocellular taurocholate uptake because mutations in these amino acids caused lack of transport; the amino acids in question are Asp115, Glu257, and Cys266 ... Turku, Finland) Results Search for conserved negatively charged amino acids, cysteines and C-terminal threonines by sequence identity An alignment of the amino acid sequence of nine SBAT proteins, ... blocks of amino acids Mutated amino acids are highlighted in gray The resulting mutants are shown in boxes, with deletions indicated (del) Fig Mutations of negatively charged conserved amino acids...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction capacity All alanine substitutions of amino acids ... charcoal-treated fetal bovine serum (Life The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS ... Functional in vivo interaction between the amino- terminal, transactivation domain and the ligand binding domain of the androgen receptor Biochemistry 36, 1052–1064 19 Ikonen, T., Palvimo, J.J &...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... Expression and purification of wild-type and mutant enzymes Full-length and truncated CpcE and CpcF proteins were expressed with N-terminal His- and S-tags using the pET-30a vector The proteins ... the amino acids in the region 238–276 are involved in the interaction of the two subunits CpcE(1–272) had only 17–28% activity of the wildtype This activity is retained if two more amino acids ... CpcF(10–213) only lost activity in the supernatant form When purified and corenatured, the mutant showed 26% and 21% enzyme activity, respectively, indicating that amino acids 1–9 in CpcF are only...
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Báo cáo y học: " Albumin dialysis improves hepatic encephalopathy and decreases circulating phenolic aromatic amino acids in patients with alcoholic hepatitis and severe liver failure" doc
... tests, arterial ammonia levels, and the concentration of total amino acids, branched amino acids, phenolic aromatic amino acids and tryptophan by high-performance liquid chromatography Briefly, ... total, branched and aromatic amino acids Results are shown in patients with alcoholic hepatitis (AH) and pruritus (control) Circulating levels of total, branched and aromatic amino acids before ... • Total amino acid and phenolic aromatic amino acids diminished and the Fischer ratio increased in patients with alcoholic hepatitis treated with MARS • Changes in amino acid levels and the Fischer...
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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation
... primary ligand can exhibit biological activity (Noorulla and Sreenivasulu, 2011; Singh et al., 2010; Freeman, 1973; Che and Siu, 2010; Podunavac-Kuzmanovic and Cvetkovic, 2007) and an amino acid ... UO2 (VI) and their biological activities J Coord Chem 57 (13), 1099–1123 Moustafa, M.E., 2005 Synthesis and structural and biological activity studies on some lanthanides chelates with O- and ... Neeraj, D., Sandeep, K., 2011 Antimicrobial Activity of Metal and Oxide Nanoparticles Interfaced With Ligand Complexes of 8-Hydroxyquinoline And Amino Acids AIP Conference Proceeding 1393 pp 173–174...
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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients
... two viewpoints – amino acids analysis and peptide isolation from CSF and thus attempt to contribute to the management of unyielding chronic pain viii Research Collaborations Some bioactivity ... allodynia and hyperalgesia The carboxy-terminal hexapeptide of NST (Glu-Gln-Lys-Gln-Leu-Gln) is conserved in bovine, human and murine species and this possesses the allodynia-blocking activity and ... using HPLC for amino acids in CSF was developed, which was then subsequently applied to quantitatively analyze all physiological amino acids especially the nine pain-related amino acids (asparagine,...
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Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions
... exhibits activity against a variety of DNA viruses and retroviruses, and is used therapeutically against human immunodeficiency virus-1 and hepatitis B virus (De Clercq E et al., 1986) 36 MRP4 and ... 1.7.2 Identification of the key amino acids By using site-directed mutagenesis, some specific amino acids important for MRP1 substrate recognition and transport activity have been identified A series ... growth and viability To gain insight into the role of key amino acid residues of MRP4 protein for transport organic anions and resistance to chemotherapeutic compounds, three aromatic amino acids...
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Bài giảng Chapter 15 Acids and Bases
... Moderate Weak Structure of Acids • oxy acids have acid hydrogens attached to an oxygen atom H2SO4, HNO3 Tro, Chemistry: A Molecular Approach Structure of Acids • carboxylic acids have COOH group ... to produce OH- ions and cations ionic substances dissociate in water NaOH(aq) → Na+(aq) + OH–(aq) • acids ionize in water to produce H+ ions and anions because molecular acids are not made ... the Brønsted-Lowry Acids and Bases and Their Conjugates in the Reaction H2SO4 + H2O ⇔ HSO4– + H3O+ When the H2SO4 becomes HSO4−, it lost an H+ − so H2SO4 must be the acid and HSO4− its conjugate...
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Tài liệu Proteins và Amino Acids trong thức ăn thủy sản pptx
... nghèo lượng) ng) Enzymes (các proteins hồng cầu ảnh hưởng điều ng nh ng hòa biến dưỡng) Plasma proteins (các proteins di động thể ng miễn dòch thể ) Hormones (vai trò điều hoà) Proteins thành phần ... hóa amino acids keto -acids Các ketoacids tham gia vào chu trình Krebs để giải phóng lượng Còn gốc NH3 cá thải bỏ qua mang, nước tiểu CH3-CH-COOH + [ O] > NH2 Alanine CH3-CH-COOH + NH3 Pyruvic ... động cân protein sinh tổng hợp tiêu hao protein cho lượng chức sinh học khác Oxy hóa amino acids Các amino acids bò khữ gốc NH3 phản ứng (oxy hóa) hay chuyển gốc amin Khữ gốc NH3 phương thức chuyển...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... alcohol series and xylene, and embedded in paraplast Seven-micrometre sections were cut and mounted on aminosilane-coated slides Sections were rehydrated, and endogenous peroxidase activity was blocked ... (5¢-CTTCCTCCGCTTCCATGA-3¢) and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC-3¢); and QsCgClp2 (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢) The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and ... critical for chitinase activity [18] is substituted by a glutamine, suggesting that this protein lacks chitinolytic activity, as was shown previously for recombinant Cg-Clp1 [5] and other CLPs [19]...
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Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx
... identified messengers coding for proteins involved in cell proliferation (MIF and cenpA), survival (i.e survivin, BI-1, and dad1), and ribosome biogenesis (members of the small and large ribosomal subunits) ... factors, hormones, and nutrients, can induce a change in the percentage of TOP mRNA associated with polysomes from 30–40% to 65–75%, and vice versa [33] Several lines of evidence converge in ... of eukaryotic 40S ribosomal proteins Mol Cell 28, 446–457 Budkevich TV, El’skaya AV & Nierhaus KH (2008) Features of 80S mammalian ribosome and its subunits Nucleic Acids Res 36, 4736–4744 Wool...
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Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf
... opposite behaviour of TAadm and TAdmdR1 strains regarding production of antibiotic suggests interaction between the actions of the Adm and DmdR1 proteins (see below) Lack of both DmdR1 and Adm proteins ... dmdR1 and adm open reading frames (hereafter named DdmdR1 ⁄ adm), and caused a deficiency in sporulation in the disrupted mutant To assess the in vivo role of the dmdR1- and admencoded proteins ... (Brevibacterium) lactofermentum [10], IdeR of mycobacteria [11] and the DmdR1 and DmdR2 proteins of Streptomyces coelicolor [12] After interaction with Fe2+ or other divalent metals, these proteins...
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