properties of extremophilic enzymes and their importance in food science and technology
Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... regulating its inhibitory properties Screening of proteases for their inhibition showed that cystatin F is different from other cystatins, both in terms of specicity and strength of binding to ... slow binding inhibitor of the cathepsins, characterized by kass values in the range of 106)107 m)1, and high kdiss values in the range of 10)3 to 10)4 for the tightly inhibited cathepsins F, K and ... enzyme Cystatins are generally rather nonselective inhibitors An interesting feature of cystatin F is the 100-fold stronger inhibition of cathepsins F, L, and V than of cathepsin S Cathepsin S is...
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Báo cáo khoa học: Human anionic trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases potx
... 15-kDa band, containing both the N- and C-terminal chains that are identical in size (band A) Activation of double-chain trypsinogen to double-chain trypsin results in the appearance of band B, ... Trypsin activity was expressed as percentage of the initial activity measured at the beginning of the incubation SDS/PAGE analysis of trypsinogens Autoactivation and degradation of trypsinogens ... zymogen in 100 lL) were trichloroacetic acid-precipitated and analyzed by reducing SDS/PAGE (12%) and Coomassie Blue staining Bands A and B correspond to the two chains of doublechain trypsin(ogen),...
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Báo cáo hóa học: " Surface properties of glass micropipettes and their effect on biological studies" pot
... from the inside wall and 3D structures of the inner wall were obtained using MeX software Figures and 10 show an FIB-milled pipette and stereo images Table shows inner surface properties of small ... achieving pipettes with desired surface properties, and may also change the way of modeling cell-pipette interactions Author details School of Mechanical Engineering, The University of Birmingham, ... milling Because of the conic shape of pipette only the very end of pipette was cut during milling process in order not to change the pipette opening significantly Ten recordings were obtained...
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Báo cáo y học: "Chondroitin and glucosamine sulfate in combination decrease the pro-resorptive properties of human osteoarthritis subchondral bone osteoblasts: a basic science study" pptx
... compelling evidence of the potential for inhibiting the structural progression of OA with CS and GS in patients with OA of the knees and hands [4-6] Moreover, the recent Glucosamine/Chondroitin Arthritis ... the result of a tightly balanced and coordinated action of bone-resorbing and bone-forming elements These elements are regulated by various factors, including cytokines, growth factors, and extracellular ... RANKL, thereby increasing the mRNA ratio of OPG/RANKL The effect of CS on OPG mRNA versus protein could be explained by the following OPG contains a heparin-binding domain to which some GAGs...
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Báo cáo y học: "Diacerein inhibits the synthesis of resorptive enzymes and reduces osteoclastic differentiation/survival in osteoarthritic subchondral bone: a possible mechanism for a protective effect against subchondral bone remodelling" ppsx
... Martin G, Bogdanowicz P, Domagala F, Ficheux H, Pujol JP: Rhein inhibits interleukin-1 beta-induced activation of MEK/ERK pathway and DNA binding of NF-kappa B and AP-1 in chondrocytes cultured in ... multinucleated TRAP-positive cells containing at least three nuclei Determination of functional metalloprotease-13 and cathepsin K Since MMP-13 and cathepsin K are produced by the murine cell line ... SAPK/JNK (p46 and p54), particularly the level of the p46 isoforms Diacerein and rhein, however, had no effect on the activation level of either kinase Figure Effect of diacerein and rhein on metalloprotease-13...
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Báo cáo y học: " Dimensionality and scale properties of the Edinburgh Depression Scale (EDS) in patients with type 2 diabetes mellitus: the DiaDDzoB study" pot
... decrease in hedonistic thoughts and an increase in sleeping problems, and to a lesser extent by an increase in feelings of panic and the feeling that things have been getting on top of oneself ... (solid lines) and males (dashed lines) depression The latent cutoff points are indicated by the vertical lines in Figures and Inspection of the individual item contributions to the total information ... of item bias) Significant differences in fit indicate gender bias for the study items and are inspected for clinical relevance To investigate the presence of DIF and to understand what kind of...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 1
... consisting of mechanical consolidation, sintering and extrusion Mechanical testing however often exhibits controversial results and the understanding on deformation behavior of fine grained/nc ... understand how these materials are sensitive to processing parameters and grain size effect on their deformation behaviors Contamination during milling and grain growth during secondary process of ... duration of its application and temperature, and crystal defects To minimize and control the level of defects and contaminants without sacrificing material properties is the major challenge in this...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 2
... recovery during milling was confirmed by the corresponding low internal strain The internal strain during milling showed inverse grain size dependence Absence of dislocations within the grains of as ... and Zr and contamination to the extent of 10% is possible in Zr- and Ti- base alloys Milling in high purity argon or in vacuum or in a reducing medium such as toluene and performing powder handling ... Hwang et al [119] In the early stage of milling, deformation by twinning and re-twinning within the grains develops sub-grain boundaries, which eventually defined nanometre-sized grains A rapid decrease...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 3
... powder increased with further milling Higher and distinct AlN peaks indicating higher 51 In- situ formation of Aluminium Nitride composite powder crystallinity of AlN particles could be observed in ... shown in Figs 3.1(a) and (b) Due to the high concentration of nitrogen in parazine, relatively fast direct nitriding reaction compared to NH3 gas can be maintained during the milling process During ... Morphology of Al powder and (b) structure of pyrazine In this study, in- situ formed AlN composite powder is obtained by milling the mixture of elemental Al with pyrazine (C4H4N2) which shows a ring-type...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 4
... increasing milling duration, broadening of XRD peaks and declining in peak intensity are observed due to the reduction in grain size and introduction of microstrain during milling In Fig 4.1(b), intensities ... strengthening from grain refinement after 10h-MM is more pronounced for pure Mg indicating grain refinement strengthening mechanism dominates in the initial milling of 10h and 20h in the composite ... samples increase near to Tm of Mg matrix (650°C) after 40h of milling indicating no apparent influence of second phases and reinforcement on melting with longer milling duration It is interesting...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 5
... following findings indicate the dominant role of grain boundary in deformation of 40h-MMed composite samples such as thermally activated unpinning of boundaries due to enhanced diffusivity in grain boundaries ... illustrated in Fig 5.4 Generally, gradual increase in ductility was observed with decreasing strain rate for all milling durations and increasing milling duration at all strain rates It is quite interesting ... explain the grain size dependence of the plastic deformation kinetics of Cu and Ag in the grain size range of 10−2 μm < d < μm In addition, GBS has been reported as deformation mechanism of nc...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 6
... atoms, (ii) grain-boundary sliding and (iii) interfacial reaction for the sink and the creation of vacancies in grain boundaries Energy dissipated in both the grain-boundary sliding and interfacial ... logarithmic coordinates of and is shown in Fig 6.1 The relationship between stress and creep strain rate is not linear at different temperatures Two outstanding features of the influence of particles ... fraction of second phase constituents, since the Mg grain size is in nanoscale and thus less likely for the reinforcement and in- situ formed particles to exist in the interior of the Mg grain, this...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 7
... grain sizes and then performing systematic tests under identical conditions of temperature and stress for the purpose of determining the values of the grain size sensitivity, and (c) performing ... temperature, and grain size is essential In the case of nc materials, this type of information may be obtained by (a) conducting creep tests over a wide range of strain rates (at least five orders of magnitude) ... pure Mg samples This finding implies that the grain refinement to a certain length scale diminishes the significance of solid solution strengthening and dispersion hardening 30hMMed composite...
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Discovery of lipid enzymes and their modulators using metabolite profiling of yeast (saccharomyces cerevisiae) mutants
... domains that can be chosen include those that are involved in lipid binding and domains involved in lipid metabolism (Table 1) Table Lipid binding domains DOMAIN FUNCTION C1 ZINC FINGER DOMAINS ... component Involved in lipid metabolism Sphingoid long chain base Diacyl glycerol kinase kinase Sphingosine Kinase Minor Sphingoid long chain Diacyl glycerol kinase kinase Sphingosine Kinase NADPH-dependent ... Tandem mass spectrometric identification of lipid species 32 Lipid profiling of single deletion mutant strains 33 3.3.1 Lipid profiling of strains in a random manner 33 3.3.2 Lipid profiling of...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... staining with Coomassie blue showed single bands for the wild-type and mutants D392N and E315M, migrating with an Mr of 63 000 (Fig 4A) In the case of the E315Q mutant, an additional faint band ... Recombinant wild-type chitinase A was obtained by cloning the chitinase A gene, lacking the C-terminal proteolytic fragment, into the pQE60 expression vector and expressing the protein in E coli ... systematically investigated, by using ESI MS, the kinetic properties of chitinase A with pNPglycosides, chitooligosaccharides, and chitin The initial velocity of the enzyme for concentrations of the...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... occurring under these conditions to be inhibited by ATP and by the protein phosphatase inhibitor NaF On the basis of this study, and subsequent studies on the action of protein kinase and protein ... ageing (thick line) and the photoconversion of remaining photo-transformable pigment (medium line) (D) difference spectra showing the regeneration of PORPChlide650 in the presence of NADPH and ... measurements of the effects of ATP, ADP, AMP and adenosine on the corresponding low pH-induced blue-shift in the absorption maximum of maize PLB, made in the presence and absence of NaF, are presented in...
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Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
... troponin and tropomyosin Tropomyosin is a thin protein strand that sits on the actin strand and, under normal resting conditions, covers the actin-myosin binding site thus inhibiting their interaction ... understanding and will be the focus and repeating theme throughout the text A sound understanding of cardiac physiology begins with basic understanding of cardiac anatomy and of the physiology of ... rises and calcium binds to troponin C, troponin I releases from actin allowing the tropomyosin molecule to be pulled away from the actin-myosin binding site This eliminates inhibition of actin-myosin...
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