... differentiation of cultured humanmesenchymalstemcells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ofhumanmesenchymalstem ... with humanmesenchymalstemcells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis ofbone marrow- derivedmesenchymal progenitor cells ... and standard plaque assay Culture ofhumanbone marrow- derived MSCs and adenoviral transduction Bonemarrow was harvested from the surgical waste of femurs of six patients, aged 48 to 63 years...
... Osteogenic Cells 46 2.8.2.2 MesenchymalStemCells 48 2.8.2.3 MesenchymalStemCells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal MesenchymalStemCells ... Chong, Z Zhang, M Choolani, J Chan Human endothelial progenitor stemcells enhances osteogenic response ofbonemarrowderivedhuman fetal mesenchymalstemcells through paracrine signalling ... Mimicking the bone- marrow niche: continuous culture of fresh bonemarrow aspirates in hypoxia enhances osteogenic differentiation ofhuman fetal mesenchymalstemcells International Society for Stem Cell...
... low numbers of adult EPCs may provide sufficient seeding cells for tissue engineering applications Bonemarrow stromal cells (MSCs) are multipotent stemcells originating from the bonemarrow stroma, ... osteogenic and endothelial cells Methods Cell Culture and Induction Bonemarrowcells were obtained from male BALB/c mice (6–8 weeks of age) Low-density bonemarrow mononuclear cells (MNCs) were isolated ... 4D) Figure Morphology of osteoblasts and endothelial cells Morphology of osteoblasts and endothelial cells During the 2nd passage, the mice bone marrow- derived mononuclear cells differentiated...
... number ofcells was determined spectrophotometrically entiation ofhumanmesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation ofhumanmesenchymalstem ... Avioli LV, Cheng SL: Regulation ofbonematrix protein expression and induction of differentiation ofhuman osteoblasts and humanbonemarrow stromal cells by bone morphogenetic protein-2 J Cell ... effects of basic fibroblast growth factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow- derivedmesenchymalstemcells J Bone Miner Res 1997, 12:1606-1614 Ogasawara...
... These data suggest the paracrine effects ofbonemarrowcells on supporting endogenous cells to regenerate However, the potential ofbonemarrowstemcells or MSCs to differentiate into IPCs ... differentiation ofhumanmesenchymalstemcells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymalstemcells ... differentiation of size-sieved stemcells into electrically active neural cellsStemCells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells...
... http://respiratory-research.com/content/9/1/25 Figure Frequency of labeled donor cells in bonemarrow Frequency of labeled donor cells in bonemarrow BM cells were harvested from recipients which were sacrificed ... Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias was considered to represent 18.1% of total murine marrow ... frequency of labeled cells was measured by flow cytometry and the total number of labeled donor cells in recipients' bonemarrow was calculated The results are shown as percentages of total number of...
... native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, the importance of nanofibers ... entrapped and buried in the bonematrix during active bone formation They are the most abundant cellsofbone tissue, making up 95% of all bonecells Relative to the other bone cells, the osteocytes ... adjoining bonemarrow The thickness of spongy bone cannot become much thicker than about 200 300 m [57,62] 2.1.1.3 Molecular structure ofboneBone is composed of several cell types and an organic matrix...
... differentiation of rat bone marrow- derivedmesenchymalstemcells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand humanmesenchymalstemcells using ... cultured humanmesenchymalstemcells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bonederivedcells in vitro: a model ofbone ... 2007;Functional network analysis of the transcriptomes ofmesenchymalstemcellsderived from amniotic fluid, amniotic membrane, cord blood, and bonemarrowStemCells (10):2511-2523 106 Rosen...
... cell-mediated mesenchymalstem cell differentiation StemCells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction ofhumanmesenchymalstemcells with disc cells: ... Multilineage potential of adult humanmesenchymalstemcells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior ofmesenchymalstemcells in the chemical ... promising cell choice is mesenchymalstemcells (MSCs) MSCs are multipotent cells predominantly found in bonemarrow that have the plasticity to differentiate into cellsof the chondrocytic, adipogenic...
... Journal of Biomedical Science 2010, 17:75 http://www.jbiomedsci.com/content/17/1/75 Page of 10 Figure Characterization ofbone marrow- derived MSCs Flow cytometric analyses ofbone marrow- derived ... with NIH image software Effect of simvastatin on the cell viability ofbone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under ... of importance for the anti-apoptotic roleof simvastatin Effect of simvastatin on the VEGF releasing ofbonemarrow -derived MSCs in vitro To assess whether simvastatin affected the release of...
... Types ofcells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential ofmesenchymalstemcells (MSCs) for bone healing 60 2.3.2 Potential ofbonemarrowderivedmesenchymal ... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiation ofbone marrow- derivedmesenchymalstemcells on nanofibrous scaffolds Journal of Bioactive and Compactable ... inflammatory cells, cellsofmesenchymal origin Chemotactic effect on other inflammatory cells, stimulation of extracellular matrix synthesis, angiogenesis, recruitment of endogenous fibrogenic cells...
... 1.5.2 Adult stemcells 10 1.5.2.1 Bonemarrowstemcells 10 1.5.2.1.1 Hematopoietic stemcells 11 1.5.2.1.2 Mesenchymalstemcells 11 1.5.3 Bonemarrowstem cell-transplantation ... cardiovascular research 1.5.2.1 Bonemarrowstemcells There are most common types ofstemcells in bone marrow: (i) hematopoietic stemcells and (ii) mesenchymalstemcells These cell types are believed ... .44 3.1 Isolating Sternum -Derived MesenchymalStemCells 46 3.1.1 Isolation ofBoneMarrowMesenchymalStemCells (MSCs) 46 3.1.2 Patient -derived cells can differentiate into osteoblasts...
... stem cells: feasibility and safety from monkey to humanStemCells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymalstemcells are capable of homing to the bonemarrow ... humanbone marrow- derived stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use BoneMarrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Humanmesenchymal ... Treatment of Femoral Head Necrosis with Arterial Perfusion ofMarrowStemCells Chin J Integr Trad West Med 2004,24(11):999-1002 Yan Z, Hang D, Guo C, Chen Z Fate ofmesenchymalstemcells transplanted...
... tendencies in mesenchymalstemcells from humanbonemarrow and umbilical cord blood StemCells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors ofmesenchymalstemcells recruit ... of mixture of β-TCP and cells revealed significantly greater area of new bone formation than control (p < 0.05) and a smaller amount of residual TCP Previous studies showed that bone marrow- derived ... for humanmesenchymal stem- cell-induced bone formation Biomaterials 2005, 26, 3631-3638 Bruder SP, Kraus KH, Goldberg VM, Kadiyala S The effect of implants loaded with autologous mesenchymal stem...
... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... whereas it was negative Figure derived adherent cells Immunosuppressive properties ofbone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte ... has been recently shown to play a role in the maintenance of the pluripotency ofhuman embryonic stemcells [40] The maintenance of pluripotency of embryonic stemcells could involve Wnt signaling...
... before [24] Most cells were in resting period of cell cycle agreeing with the characteristics ofhumanbone marrow- derivedmesenchymalstemcells [5] The Yang et al Journal of Biomedical Science ... Multilineage potential of adult humanmesenchymalstemcells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Humanmesenchymalstem cells: progenitor cells for cartilage, bone, fat and ... ofmesenchymalstemcellsderived from bonemarrow and adipose tissue J Cell Biochem 2006, 99:1285-1297 Kern S, Eichler H, Stoeve J, Kluter H, Bieback K: Comparative analysis ofmesenchymal stem...
... infection ofhumanbonemarrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential ofhumanbonemarrowmesenchymalstemcells ... evaluate adipogenic potential of amniotic fluid -derived humanmesenchymalstemcellsStemCells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Humanmesenchymalstemcells and their use in cell-based ... [39,40,43] disclosed that HIV productively infected bonemarrowmesenchymal or stromal cells to different extents Recent data on bone marrowderived MSCs demonstrated that HIV-1 proviral DNA integration...
... Lim B Identification of common pathways mediating differentiation ofbone marrowand adipose tissue -derived humanmesenchymalstemcells into three mesenchymal lineages StemCells 2007 Mar;25(3):750-60 ... et al The in vitro migration capacity ofhumanbonemarrowmesenchymalstem cells: comparison of chemokine and growth factor chemotactic activities StemCells 2007 Jul;25(7):1737-45 PubMed PMID: ... ACI, and microfracture (265, 266), or introducing new cell sources like debrided waste chondrocytes, BoneMarrowderivedMesenchymalStemCells (BM MSCs), or any combination of these cells (29,...