production as measure of cell mediated immune response primed by in vivo cytosolic delivery
Báo cáo y học: " Phytol-based novel adjuvants in vaccine formulation: 1. assessment of safety and efficacy during stimulation of humoral and cell-mediated immune responses" doc
... immune responses Although in the past most vaccines have been designed to stimulate antibody responses, vaccines currently in development are increasingly designed to elicit cellular immune responses ... shown) Interestingly, the 2° anti-phthalate antibody response was boosted as effectively by PHIS-01 and phytol adjuvants as by CFA/IFA combination or RAS In contrast, TiterMax and Alum were ineffective ... effector T cells Since induction of cytolytic effector cells is pivotal in ensuring the success of tumor vaccines, we investigated relative efficacy of phytols and known adjuvants in their ability...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
... belonged to immune response, which had the lowest EASE score (9.89E-11) The genes in signal transduction corresponded to intracellular signaling cascade, protein kinase cascade, and activation of mitogenactivated ... usage, including disease-modifying agents and steroids, and laboratory testing, including rheumatoid factor (RF) and C-reactive protein (CRP), of the nine RA patients are shown in Table None of the ... affect the role of polo-like kinase in mitosis, the role of CHK protein in cell cycle checkpoint control, and affect pyrimidine metabolism, and ataxia telangiectasia mutated (ATM) signaling There were...
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Báo cáo y học: "Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice" pptx
... HCV-antibody response IFN-g intracellular staining CD8+ CTL responses were assessed by measuring the mouse IFN-g production using intracellular staining The intracellular procedures were done according ... peptides, as indicated by the decrease in the CFSE stain intensity As the cells proliferate, the cell population shifts to a lower intensity due to the decrease of staining in the cell membranes of ... proliferating cells Daughter cells have half the fluorescent intensity of the parent cells (Figure 2) CD8+ T cell cytolytic activity was demonstrated by INF-g production using intracellular staining...
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Báo cáo y học: "Resolution of cell-mediated airways diseases Carl G Persson*1 and Lena Uller2" pps
... epithelial base paraepithelial crawling of the leukocyte may begin by integrin binding to desmosomal junction adhesion molecules Several binding interactions and cellular signalling events including cytosolic ... explain observations in vivo of airway wall neutrophilia induced by steroid treatment in both COPD [51-53] and asthma [5456] However, as with steroid-induced increase in apoptosis of eosinophils ... stage of COPD was associated with reduced numbers of mast cells in the airway wall compared to less severe COPD This difference could not be explained by increased apoptosis of airway wall mast cells...
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Báo cáo y học: " Characterization of the innate immune response to chronic aspiration in a novel rodent model" ppsx
... stained using hematoxylin and eosin as well as Masson trichrome stain for collagen The extent of fibrosis in trichrome-stained specimens was graded by a pathologist in a blinded fashion using ... http://respiratory-research.com/content/8/1/87 Figure Cytokine response as a function of duration of gastric fluid aspiration Cytokine response as a function of duration of gastric fluid aspiration Cytokine levels in bronchoalveolar ... chronic aspiration may lower the threshold for an immune -mediated asthmatic response by inducing isotype-switching to IgE production in B cells [24] Figure gastric fluid TGF-beta production in response...
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Báo cáo y học: "Bench-to-bedside review: Biotrauma and modulation of the innate immune response" pps
... depletion (vinblastine injection) has been shown to attenuate IL-8 increase in the lung P-selectin or intercellular adhesion molecule-1 (key cell membrane proteins that are involved in endothelial cell ... PMN-induced changes in microvascular permeability in the lung The ability of neutrophils to cause lung damage was mediated by increased expression of CXC chemokine receptor (CXCR)2 ligand in lung ... correlation between changes in soluble Fas ligand (a key mediator of cellular apoptosis) levels and changes in creatinine in patients with ARDS involved in a clinical trial of protective ventilation...
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Báo cáo y học: " The role of the humoral immune response in the molecular evolution of the envelope C2, V3 and C3 regions in chronically HIV-2 infected patients" pdf
... from that of HIV-1 Glycans on HIV-1 envelope protein play an important role in the folding of the glycoproteins, in infection and in evasion from the host immune response (reviewed in [69]) We ... Albert J: Plasma viral load in HIV1 and HIV-2 singly and dually infected individuals in GuineaBissau, West Africa: significantly lower plasma virus set point in HIV-2 infection than in HIV-1 infection ... diversity and divergence from the infecting strain increase during HIV-1 infection but become stable or even decrease in the advanced stage of disease, with the lower CD4+ T cell counts and progression...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 1
... proposed, including cell printing and cell sheet engineering In this section, I will review some of the existing and emerging strategies currently applied in TE Chapter Introduction 1.1.1 Classical ... exerts inhibitory effects on the myosin-actin interactions Other molecules that are involved in the signalling pathway include mysin light chain kinase and G-protein receptor lingands, such as angiotensins ... 2006) Cell printing thus refers to the deposition of cells into three-dimensional structures, which may lead to self-assembly to form engineered tissue, as recently shown in the bioprinting of skin...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2
... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies were generated ... from cord blood (a, b) In contrast, outgrowth colonies from fetal blood (c) were heterogeneous in nature, adopting morphologies ranging from cobblestone, fibroblastic to spindle (d - f) 123 ... Isolation and Characterisation Of Vascular Progenitors (a) (b) (c) (d) (e) (f) Figure 4-4: CFU assay Adherent colonies appeared after ten days of culture, and were stained with crystal violet to...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 3
... serine peptidase 1, insulin-like growth factor binding protein 4, suppressor of cytokine signalling 2, insulin-like growth factor binding protein 6, phospholipase C an dubiquitin conjugating ... containing FYVE, RhoGEF and PH domain containing GATA binding protein hematopoietically expressed homeobox insulin-like growth factor binding protein interleukin jagged (Alagille syndrome) keratin ... homolog In contrast, UCB-EPC are largely involved directing cell differentiation, as well as processes involving regulation of cell growth and size The common genes involved in regulation of cell...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 4
... was more heterogeneous in my CFU-assays, comprising both cobblestone-shaped cells, as well as fibroblastic and spindle shaped cells, indicating that multiple cell types are generated following ... blood response, including the size of the clot,, an indication of consumption of clotting proteins Classical cigar-shaped plots could be generated in response to each group with no enhanced fibrinolysis, ... consumption by the material surfaces Although no significant difference in the rate of clotting, as defined by A°, was measured, the trend appeared to be inversely related to that of the observed...
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Children security as part of the " living together with floods" strategy in the Mekong river delta, VietNam
... trained There is no official swimming training in the school program in the Mekong River Delta It seems an irrational structure for children living in a floodplain as the Delta In the flood seasons, ... Diversifying the crop production calendars Widening/deepening drainage channels Finding the solution for children security Raising evacuation of public infrastructure and housing foundations Providing ... to the lack of swimming trainers, financial support, training facilities and tools as swimming pools, buoyant items It is suggested that in preparation for the coming flood seasons, mass organizations...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... triticale seedlings [27] Macropinocytosis dependent transduction of fluorescent proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion ... colour in the merged image The complex size was determined using the IMAGEJ software A a 20 15 10 as well as by fluorimetric analysis In mammalian cells, reports pertaining to a plasma membrane -mediated ... deliver plasmid DNA in plant cells GUS gene expression was further enhanced by the addition of cationic transfecting agent LipofectamineÔ 2000 Very recently, AID -mediated delivery of plasmid was demonstrated...
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Báo cáo lâm nghiệp: " Predator/prey ratios: a measure of bark-beetle population status influenced by stand composition in different French stands after the 1999 storms" pps
... higher in 2002, decreased in 2003 and increased again in 2004 (Fig 1) The sudden decrease in T formicarius catches in 2003 might perhaps be explained by the heat wave that struck France in July ... species) As the number of tree species increases, this index produces values ranging from (when the landscape is composed by only one tree species) to infinity [15] The calculation of this index was ... 2L among years was the strongest in Hanau (P = 0.001) and Steinbach (P < 0.0005) The T formicarius catches increased from 2001 to 2002, then decreased in 2003 and increased again in 2004 (Fig 1)...
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báo cáo khoa học: "Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins" potx
... significant increase in inhibition of cell associated HIV-1IIIB/H9 virus infection The use of this cocktail resulted in 60 to 71% inhibition of cell associated virus infection All four antibodies used in ... the exact reason behind increased inhibition of cell associated virus v/s cell free virus We assume it may be due to better binding between cell associated virus and AgNPs The use of AgNPs+ all ... cell associated both viruses are present in the infectious inoculums in real life, any increase in inhibitory potencies of NABs against HIV-1 IIIB/H9 virus infection will be interesting In our...
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Báo cáo y học: " Immune response to allergens in sheep sensitized to house dust mite" potx
... HR: cDNA sequence of two sheep mast cell tryptases and the differential expression of tryptase and sheep mast cell proteinase-1 in lung, dermis and gastrointestinal tract Clin Exp Allergy 2000, ... Figure gens and mast cell counts in skin of control and HDM-sensitized sheep following intradermal challenge with HDM allerEosinophil Eosinophil and mast cell counts in skin of control and HDM-sensitized ... cells were detected in paraffin sections by immunochemical staining [23] using a polyclonal rat anti-ovine tryptase antibody, kindly provided by Prof Hugh Miller (University of Edinburgh, UK); all...
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Báo cáo y học: " Characterization of the bronchodilatory dose response to indacaterol in patients with chronic obstructive pulmonary disease using modelbased approaches" ppt
... dose response changed with baseline FEV As baseline FEV increased, both the steepness and the maximum of the dose response increased In particular, the lowest quartile, with a baseline FEV of ... strongly influenced by the baseline FEV1 In other words, as disease severity increases (i.e baseline FEV1 decreases), patients require higher doses Figure Left: patient-level dose response data by baseline ... while providing further insights into the impact of patient characteristics on dose response The key finding of the patient level analysis was that baseline FEV , as a marker of disease severity,...
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Báo cáo khoa học: " Yellow-necked mice (Apodemus flavicollis) and bank voles (Myodes glareolus) as zoomonitors of environmental contamination at a polluted area in Slovakia" doc
... increase the heavy metal content of mammals inhabiting the polluted areas In general, there is a significant relationship between the amount of heavy metals in the environment and in the organs of ... hierarchy of Cd concentrations in the tissues was kidney > bone > liver > muscle In our study, the highest concentration of Cd was detected in the bone of bank vole followed by liver and kidney In addition, ... of Cd were found in bone followed by kidney and liver The hierarchy of Cu and Fe concentrations was kidney > liver > bone in yellownecked mice In the bank vole, highest concentration of Cu was...
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Báo cáo y học: "MHC class I expression protects rat colon carcinoma cells from hepatic natural killer cell-mediated apoptosis and cytolysis, by blocking the perforin/granzyme pathway" ppt
... cytolysis in CC531s cells was induced by the binding of mAb OX18, CC531s cells were incubated with mAb OX18 alone After 3, 24 or 48 hours of incubation, no apoptosis or cytolysis in CC531s cells was ... inhibition by DCI, Ca2+ chelation by EGTA and caspase inhibition by Z-VADFMK When hepatic NK cells were preincubated with DCI, a granzyme inhibitor in intact cells [30], hepatic NK cellmediated ... perforin/granzyme pathway This mechanism of immune escape could possibly contribute to the incomplete killing by hepatic NK cells of arriving colon carcinoma cells in the liver sinusoids, resulting...
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