Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 4

Chapter 5. Functionalisation of µXPCL Surfaces Using Radio Frequency Glow Discharge (RFGD) Plasma unpaired t-test) (Figure 5-2). After repeating the grafting process, water contact angle was found to drop further to 52.0 ± 1.3°, in line with the above observation (p[...]... sloughing in vivo, thus the underlying material must be designed to be blood compatible These factor led to my selection of CD 34 antibodies as a suitable conjugand, and I detail here my studies on cell adhesion, as well as blood compatibility Finally, I studied the assembly of cells and film to form a layered structure The tunicae in vascular tissue are unique in the composition of cells: each compartment... through conjugation of biomolecules These developments contribute towards improved biocompatibility of the tissue engineering scaffolds In the specific context of vascular tissue, two other important classes of tissue responses remain First, possible issues of thrombogenesis must be addressed Acute failures of vascular graft occurring within the first three months of implantation are caused by technical complications,... CD 34 antibody reduced contact activation, and thus suggesting that blood compatibility had been enhanced This result was borne out by blood compatibility index assay, showing PCL-CD 34 to have superior blood compatibility I then proceeded to study the cellular response to the surface I identified that PCLPAAc is suitable for perivascular cells, and PCL-CD 34 to be suitable for endothelial progenitor cell. .. assay *** denotes significantly increased carboxyl density over pristine PCL films (p . significant drop was observed as a result of plasma treatment, nor was their any trend to suggest mechanical compromise, irrespective of the number of rounds of PAAc grafting. In light of the. survey XPS scans show increased O:C ratio following PAAc grafting, and then the introduction of an N1speak following the conjugation of the CD 34 antibody, indicating presence of the protein Functionalisation of µXPCL Surfaces Using Radio Frequency Glow Discharge (RFGD) Plasma 1 74 after thorough repeated washing. My results confirm the feasibility of the use of plasma based methods for the