procedures for derivation and characterisation of human embryonic stem cells from odense denmark
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... somatic cells of the gonad and become irreversibly committed as germ cells Human Embryonic Germ (hEG) cells, also a form of stem cells are isolates of PGCs from the developing gonadal ridge of to ... prove informative for the in vitro manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno-Free Support Systems and in the Presence of Xeno-Free...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure ... Sumitha, Aji and Tamanna, and Susan, thank you for the joy you bring to my life and my world Pappa and Mamma, thank you for your love and unconditional support My grandparents and the rest of my extended ... GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Summary V List of Tables VII List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 ... Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst stage ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and...
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Baculovirus mediated genetic modification of human embryonic stem cells
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... cells from the adult tissue (autologous or allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded ... differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and ... METHODS Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used in this study were the H1 cell lines (isolated and established at the University of Wisconsin)...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from ... differentiation and tissue engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells ... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells...
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Establishment of autologous culture systems for human embryonic stem cells
... their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical ... hESCs for prolonged periods in culture See Figure for schematic diagram of derivation of hESC lines from a human blastocyst Figure Schematic diagram of derivation of hESC lines from a human blastocyst...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... specification of the neuroectoderm (Muñoz-Sanjuán and Brivanlou, 2002) Bmp are secreted from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... literature review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable ... use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced pluripotent stem (iPS) cells ii Chapter II Culture and Propagation of H9 ... of stem cells, adult stem cells (also named as somatic stem cells) , and embryonic stem cells[ 5] However, a third type of pluripotent cell was introduced in 2007 through genetic manipulation of...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their guidance, ... particular, thank you to Prof Too Heng-Phon for providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract ... Culturing of Mouse Embryonic Stem Cells (mESC) a Culturing Conditions Initial culturing methods for mESC required a layer of co-cultured fibroblasts to provide a biochemical and physical support for...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time ... regions of the LCR (HS2 and HS3) but not at the ec- and bmajor-globin genes in undifferentiated ES cells (day 0, Fig 3) indicating that dimethylation of H3K4 and recruitment of RNA Pol II and TBP...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from these expanded cells to obtain ECs for transplantation (Figure 1) The ECs derived from human ES cells (hES-ECs) ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... muscle actin; hESECs+MCs: a mixture of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hESMCs: MCs derived from human ES cells http://www.translational-medicine.com/content/6/1/54...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells ... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells ... approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that...
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Báo cáo y học: "HIV-1 Capsid Assembly Inhibitor (CAI) Peptide: Structural Preferences and Delivery into Human Embryonic Lung Cells and Lymphocyte" pptx
... membranes and understand better their pharmacological behaviour For delivery of the HIV-1 CAI into human cells a bi-modular peptide was developed and constructed consisting of a transport unit for ... N-terminus of p55 is myristoylated triggering its association to the cytoplasmic side of cell membranes [7] and the release of the budding of viral particles from the surface of infected cells After ... capable of forming significant amounts of α-helical conformation and it is induced to this at relatively low concentration of TFE The three control peptides never formed large stretches of α-helical...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... and AR50249 from to National Institute of Arthritis and Musculoskeletal and Skin Diseases to SCG and CHE, by grant STE 1051/2-1 from the Deutsche Forschungsgemeinschaft (DFG) to AFS and UN, and ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... and the surface areas of 10 randomly chosen lacunae by two independent investigators (AFS and BP) in a blinded fashion were measured from each of three representative microscope views taken from...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and ... done for stage IV cells Quantification of Akt (C) and ERK (D) phosphorylation shows PD98059 and LY294002 increase the activation of Akt and ERK, respectively Quantitative RT-PCR for (E) insulin and...
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báo cáo khoa học: " Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius" docx
... sites, for example Ara h3 contains eight distinct epitopes and most of these differ from the corresponding regions of other legume and tree-nut allergens [41] Identification of specific allergens and ... Casarotto and Elaine Smith for valuable technical assistance and Lars Kamphius and T.J Higgins for helpful comments on the manuscript This work was supported by CSIRO and the Centre of Food and Genomic ... fixation and uptake of N from the soil [26] Proteins involved in cell division are abundant during early stages of seed development, and their level decreases before the accumulation of the major...
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