potential for using isolated enzymes
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 19 pot
... place; and its return to the original site C Potential for Using Isolated Enzymes As previously stated, microorganisms are among the main agents for the transformation and degradation of organic chemicals ... EXAMPLES OF USING ENZYMES FOR BIOREMEDIATION As evident from the previous subsections, many enzymes that are able to transform hazardous substances are now available in a purified form Catalytic, ... biochemical features are known for several of these enzymes Studies have been dedicated to the use of these enzymes for practical applications Both free enzymes and enzymes immobilized on diverse...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... systems with high hydrolytic potential for cellulose degradation [1] Hence, the search for and discovery of novel thermostable enzymes with enhanced capabilities for cellulose degradation may ... individual microorganisms: 27 kDa for a Thermotoga maritima cellulase [58]; 45 kDa for a B licheniformis xylanase [59]; 60 kDa for a Bacillus sp cellulase [60]; and 75 kDa for a recombinant E coli xylanase ... due to their potential use in the production of value-added bioactive compounds such as enzymes and antibiotics [12, 13] Thermophilic cellulase-producing microorganisms have been isolated from...
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Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx
... values ‡ 13 lm for K-562 cells [16]), as the dimeric BS-RNase (IC50 $ lm for malignant SVT2 fibroblasts [14]) and artificially domain-swapped RNase A (ds-RNase A) dimers (IC50 $ 0.5 lm for HL-60 cells ... for the two interacting molecular surfaces, performed with the program sc from the ccp4 suite [27] The obtained sc value of 0.400 indicates weak binding of the two entities of the RATE Therefore, ... R T Raines (University of Wisconsin-Madison, WI, USA) for providing the plasmid for RI The Land Sachsen-Anhalt is gratefully acknowledged for supporting this work (3537C ⁄ 0903T) A Schierhorn,...
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classes of groups and their properties
... turns out that the composition formations are exactly the Baer-local formations ([She97]) Any function f : P −→ {formations} is called a formation function Given a formation function f , we define ... isomorphic to J is a formation We call this formation the Baer-local formation defined by f , and we denote it by BLF(f ) A class B is called a Baer-local formation if B = BLF(f ) for some Baer function ... Schunck classes of the form H = EΦ F, for some formation F, among the Schunck classes for which every group which is not in H possesses H-critical subgroups Theorem 2.3.24 For a Schunck class H,...
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Báo cáo hóa học: " Hydrothermally Grown ZnO Micro/Nanotube Arrays and Their Properties" pptx
... the formation of micro/nanotubes from small nanowires has already been observed for several materials, such as ZnO and ZnS [13, 41] The crystal structure of the products was also investigated using ... micro/nanotube arrays was measured by using a Dataphysics OCA20 contact angle system at ambient temperature All the electrical measurements were carried out using a semiconductor parameter analyzer ... performed experiments with short reaction time Figure 1f is a SEM image of the product obtained with a reaction time of h High-density ZnO nanowires with diameters smaller than 100 nm are formed...
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COMPOSITES AND THEIR PROPERTIES ppt
... or publisher No responsibility is accepted for the accuracy of information contained in the published chapters The publisher assumes no responsibility for any damage or injury to persons or property ... of Continuous Fiber Reinforced Composites The chapter comprehensively presents a work about an authors’ model for simulating the damage evolution of continuous fiber reinforced composites under ... properties of composites made from polyester resin-matrix and Kevlar reinforced fiber and ramie reinforced fiber are reported by performing a massive amount of experiments Chapter 13 The Chosen Aspects...
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Enzymes and Their Inhibition ppt
... does not extend to copying for general distribution, for promotion, for creating new works, or for resale Specific permission must be obtained in writing from CRC Press for such copying Direct all ... been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use Neither ... Janet Conway Enzymes and Their Inhibitors: Drug Development Edited by H John Smith and Claire Simons © 2005 by CRC Press TF1766_title 7/22/04 2:53 PM Page CRC Enzyme Inhibitors Series Enzymes and...
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Discovery of lipid enzymes and their modulators using metabolite profiling of yeast (saccharomyces cerevisiae) mutants
... on ice for 30 Following which the cells were heat shocked at 37° C for 20 sec and incubated on ice for About 200 µL of LB media was added and the transformation mix was incubated at 37°C for hour ... innoculum for subsequent culture All strains for analysis were grown in a total volume of 12 ml using 50 ml falcon tubes The cells were grown to an O.D of 0.8-0.9 and harvested at 3500 rpm for The ... mix was added and vortexed for 30 sec followed by incubation for 30 sec This step was followed by the addition of 400 µL of Chloroform and 300 µL of 1M HCL and vortexed for 30 sec The extraction...
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Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
... [10,11] The inhibition of fungal Man-ol production therefore presents a potential target in advanced therapies for A fumigatus infections Unfortunately, little is currently known about the enzyme ... of AfM2DH for reactions with different polyol and ketose substrates Km and kcat Km data for carbonyl substrates are corrected for the available proportion of open-chain free-carbonyl forms present ... values for ATP, ADP and AMP from Table 3) were used to construct free energy proles for the transformations catalyzed by AfM1PDH and AfM2DH These free energy proles (Fig 2) show that both enzymes...
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Tài liệu Materials, Their Properties and Uses doc
... m/s), and are used especially for thread grinding and cutoff wheels Shellac binder is used for light work and for high finishing Rubber is used for precision grinding and for centerless-feed machines ... vacuum forming Resins have been developed especially for cold forming or stamping from extruded sheet Typical applications are helmets, refrigerator liners, luggage tote trays, housings, grills for ... tartaric and formic acids and is used for making resins and plasticizers Thioacetic acid has the formula of acetamide but with HS replacing the NH2 It is a pungent liquid used for making esters for synthetic...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... surface are flexible, with no distinct conformation Refinement of ChiG with zero occupancy for residues 170–183 confirmed the (slightly) different conformation for ChiG in the 178–183 region, as judged ... alignment was made using the protein structure comparison service SSM at the European Bioinformatics Institute (http://www.ebi.ac.uk/msd-srv/ssm [45]) Four major deletions in the bacterial enzymes are ... assignments are indicated with h for a-helix and s for b-strand The consensus helix comprising residues 169– 177 in ChiG is extended towards Cys183 in the other three enzymes the substrates remained...
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Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
... following conditions: Hot start for at 95 °C; followed by 20 cycles consisting of 95 °C for 30 s, 70 °C for and 72 °C for min; and terminated by an extension step for at 72 °C The reaction mixture ... resulted in pPIC9 Phl p N and Q, which were used for transformation after linearization with BglII Mutagenesis of His104 to Val was performed by PCR using the primer phlp1s (5¢-ACC CGGGAGGAGGAATCCCCAAGGTCCCCCCCG-3¢) ... were released using the restriction enzymes SmaI and SnaBI or EcoRI and SnaBI, respectively, and both ligated in pBS, which had previously been linearized using the restriction enzymes EcoRI...
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STAINLESS STEELS their properties and their suitability for welding
... responsibility for errors or for information which is found to be misleading Suggestions for or descriptions of the end use or application of products or methods of working are for information only ... give advice on the selection of filler metals for different steel grades – inform briefly on different filler metal forms – provide practical advice for the welding of stainless steels Ferritic ... thereof Before using products supplied or manufactured by the company the customer should satisfy himself of their suitability Information 270502GB; reprint of inf 9473:2 Teknisk information/Edita...
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Materials, Their Properties and Uses potx
... m/s), and are used especially for thread grinding and cutoff wheels Shellac binder is used for light work and for high finishing Rubber is used for precision grinding and for centerless-feed machines ... vacuum forming Resins have been developed especially for cold forming or stamping from extruded sheet Typical applications are helmets, refrigerator liners, luggage tote trays, housings, grills for ... tartaric and formic acids and is used for making resins and plasticizers Thioacetic acid has the formula of acetamide but with HS replacing the NH2 It is a pungent liquid used for making esters for synthetic...
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facile route to straight sno2 nanowires and their optical properties
... nanowires were fabricated by a simple chemical vapor method using SnO2 nanoparticles as starting materials These straight nanowires are uniform in diameter and length, and grow along the unusual ... further determined by XPS spectra (Fig 3) The binding energy centered at 530.75, 486.88, 495.38 eV for O1s, Sn3d5/2 and Sn3d3/2 , respectively, are in good agreement with the value of the bulk SnO2 ... the Sn3d and O1s peaks gives an average Sn/O atomic ratio of 1:1.95, indicating the O-deficient formation of the SnO2 nanowires Fig XRD pattern of the SnO2 nanowires Fig XPS spectra of the obtained...
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Tensile properties of cooked meat sausages and their correlation with texture profile analysis (TPA) parameters and physico chemical characteristics
... values with different letter differ significantly (p < 0.05): a, b for BS; a, b for Hard; x, y, z for Adh; v, g for Coh; k, l for Spr were observed between the textural profiles and Textural profile ... textural analyzer Rupture force was taken as the maximum force peak height (N) required for breaking the sample Breaking strength (N/cm2) was obtained dividing the rupture force by the cross-sectional ... (Bourne, 1978): hardness (N) maximum force required to compress the sample, springiness (m), ability of the sample to recover its original form after deforming force was removed, adhesiveness (N...
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Báo cáo hóa học: " A systematic review of mobility instruments and their measurement properties for older acute medical patients" doc
... abstract for data on clinimetric properties of relevant instruments Hard copies of potentially relevant papers were obtained If a reason for instrument exclusion (criteria described for the phase ... scored for successfully completing each item Items sum to obtain a maximum score of In the level PPME scoring system, zero is scored for a fail, one point for a low pass and two points for a high ... provided Baseline SD 2.1 for sample (n = 146) and 1.7 for sample (n = 352) Weighted average SD = 1.8 0.18 0.42 Pooled SD not provided Baseline SD 2.8 for sample (n = 146) and 3.1 for sample (n = 352)...
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Báo cáo hóa học: " Surface properties of glass micropipettes and their effect on biological studies" pot
... will make the chamber warm and gradually decrease the heating time for subsequent pipettes For this reason the chamber was left for to cool down after pulling every five pipettes To test the reproducibility ... cells (HUVECs) were used for patch-clamping experiments HUVECs were cultured in Endothelial Basal Medium (EBM, CC-3121, Lonza, Basel, Switzerland) on coverslips for to days before the experiments ... 15) Higher seal resistance for polished pipettes could be explained by their better sealing potential Contact area between pipette tip and cell membrane is higher for polished pipettes and since...
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Báo cáo hóa học: " Review of thermo-physical properties, wetting and heat transfer characteristics of nanofluids and their applicability in industrial quench heat treatment" pot
... monitoring of polymer quenchant is required for optimal performance and it is not suitable for steels requiring high temperature quenching [7] Therefore, it is necessary to develop new type of ... is a need for development of nanofluids having (i) high quench severity for enhancement of heat transfer for thick sections with low quench sensitivity and (ii) low cooling severity for thin sections ... austenite may transform to ferrite, pearlite, bainite or martensite depending on the cooling rate Phase transformations in solid state are accompanied by volume variation and transformation plasticity...
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Báo cáo hóa học: " Fe3O4–Au and Fe2O3–Au Hybrid Nanorods: Layer-by-Layer Assembly Synthesis and Their Magnetic and Optical Properties" pptx
... products, and finally dried at 80°C in air For the synthesis of a-Fe2O3–Au nanorods, the as-prepared FeOOH–Au nanorods were heated to 500°C for h in air While for the synthesis of Fe3O4–Au nanorods, ... layer-by-layer assembly Briefly, 10 mg FeOOH nanorods was sonicated for h in 50 ml M NaCl solution, and 80 mg PAH was added and stirred for 0.5 h Subsequently, the excess PAH was removed by six repeated ... FeOOH–Au nanorods were heated to 400°C for h under H2/Ar (10% H2) atmosphere Characterization The obtained samples were characterized by X-ray powder diffraction (XRD) using a Rigaku D/max-ga X-ray diffractometer...
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