poly ethyleneimine and polyfectinmediated membrane damage and apoptosis in human cell lines
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... cytotoxicity against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro ... selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the microtiter tetrazolium method [41–43] The cytotoxicities of the compounds in each cell line are shown in Fig ... compounds in some human cell lines and PMNs Cytotoxicity was determined by microtiter tetrazolium assay ‘Control’ contained cells plus cell culture medium supplemented with 2% fetal bovine serum...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original TetR instead ... variety of human cell lines; primate and canine cell lines may also be used [5] It has been extensively documented that the plasmids are maintained episomally (5–10 copies per cell, e.g for 293 cells), ... results for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference experiments,...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... melanoma cells to each drug alone, both drugs were combined in constant ratios to each other Among the 11 BRAFV600E mutant cell lines, in cell lines the combination was antagonistic and in cell lines ... Combination index for the combination of μM vemurafenib and 10 mM metformin of all melanoma cell lines tested The green cell lines harbor BRAFV600E, red shows NRASQ61 mutant cell lines, and black ... representative cell lines is included in Additional File and a time course analysis in Additional File A summary of the key findings focusing on the 24 hour time point in BRAFV600E mutant cell lines is...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... H2O2-induced apoptosis in transfected C2C12 cells Furthermore, there was no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding ... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore, ... interaction between HSP70 and Smac HSP70 contains three functional regions: the ATPbinding domain, the peptide-binding domain, and the EEVD motif Although the EEVD motif is considered to be involved...
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Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
... for Zn-induced apoptosis in human Burkitt lymphoma B cells An increase in labile Zn in the cytoplasm may suppress apoptosis by inhibition of the action of caspase, cytoplasmic apoptosis inducer, ... caspase via activation of p38MAPK using a Zn ionophore in human leukemia HL-60 cells An acute increase in the intracellular Zn level caused cytotoxicity in an intracellular Zn level-dependent manner ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response...
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Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx
... potential link between inflammation and hyperplasia during OA and RA in the joint [61] The use of a specific NF-κB inhibitor has been reported to result in a significant decrease in joint swelling in ... IKK-β, and IKK-γ quently they may also influence inflammatory and apoptotic pathways using other mechanisms Further in vitro and in vivo studies in animals and humans will be required to determine ... production and upregulation of proinflammatory cytokines and enzymes such as Cox-2 and MMPs, which in turn produce prostaglandins and degrade ECM macromolecules leading to cartilage degradation and...
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báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway" pptx
... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... has been reported in a few papers Both in vitro and in vivo studies showed that Osthole possessed an anticancer effect by inhibiting human cancer cells growth and inducing apoptosis [13-17] It ... Gupta S, Afaq F, Mukhtar H: Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells Oncogene 2002, 21:3727-38...
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báo cáo khoa học: "Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251" pdf
... understanding of the underlying mechanisms of AdbFGF-siRNA-induced growth suppression and apoptosis of glioma cells Materials and methods 2.1 Cell Culture and Adenovirus Infection The human glioblastoma ... glioma in vitro and in vivo bFGF exerts functions via its specific binding to the high affinity transmembrane tyrosine kinase receptors [23] and the low affinity FGF receptors (FGFR1-4) [24] The binding ... Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical Vein Endothelial Cells JBC 2002, 277:21237-21245 Chan SL, Yu VC: Proteins of the bcl-2 family in apoptosis signaling:...
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Báo cáo y học: " Histone deacetylase inhibitors induce apoptosis in human eosinophils and neutrophils" docx
... TSA-induced apoptosis in human eosinophils (n = 6, data not shown) Involvement of caspases in TSA-induced apoptosis in human eosinophils Even though the involvement of caspases in apoptosis in ... apoptosis in macrophages (Figure 12) Discussion In the present study we show that HDAC inhibitors inhibit HDAC acitivity and induce apoptosis in human eosinophils and neutrophils in the absence and ... http://www.journal-inflammation.com/content/7/1/9 is higher in eosinophils than in neutrophils The mechanism of apoptosis- enhancing action of HDAC inhibitors in human eosinophils seems to involve JNK and caspases and HDAC inhibitors have...
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Báo cáo khoa học: "Cysteine protease activation and apoptosis in Murine norovirus infection" docx
... remained viable and morphologically indistinct from control cells Blebbing, swelling, and cytoplasmic leaking were evident in cells infected in the presence of the caspase inhibitor (Figure 4) In ... RAW264.7 cell morphology changes during MNV-1 infection RAW264.7 cell morphology changes during MNV-1 infection Brightfield cell images of mock-infected (A) and infected (B) cells 12 hpi The cells in ... PCD using survivin and cathepsin B is involved This program is initiated within hours of infection, leading to eventual cell death 24-36 hpi In the presence of a pan-caspase inhibitor, cell death...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx
... may occur during the initial h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, and examined the cells under ... to fully understand HSV-1 infection of all target cells, including RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies ... entry into RPE cells V Tiwari et al Fig Role of nectin-1 during HSV-1 entry into RPE cells (A) Antibody against nectin-1 significantly inhibits HSV-1 entry into cultured RPE cells Cells (indicated)...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
... measuring the binding of [125I]Streptavidin Specific binding at each point was normalized with reference to specific binding before the incubation at 37 °C (0-min point) to derive the fraction of initial ... (0–60 min) at 37 °C The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [125I]Streptavidin In the absence of bTSH, a very weak internalization ... After washing the cells, the concentration of receptor-Ig complexes at the cell surface was quantified by measuring the binding of [125I]Streptavidin [30] No Ig binding was observed in cells transfected...
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Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt
... from the indicated cell lines was subjected to semiquantitative RT-PCR with TSP1 primers HNK, normal cell strain; P769, PRC9, SW839, ARZ-1 and WT8, RCC cell lines (E) The same cell lines were ... + +++ ++ In contrast, HNK and RCC cell lines differed in their ability to induce angiogenesis Conditioned media (CM) from P769 and other RCC cell lines were potently angiogenic in rat and mouse ... CM and cell lysates (CM and L, respectively) were collected and TSP1 was detected by western blotting Note the low TSP1 secretion and higher intracellular levels in RCC cells (F) HNK and P769 cells...
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Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf
... biotinylated lysines in histone H3: N1)9 (including K4 and K9), N9)16 (including K9 and K14), N16)23 (including K18 and K23), and N18)25 (including K18 and K23); subscripts denote the amino acid ... alanine (peptide R8A6)13) biotinylation increased considerably, suggesting that unmodified arginines interfere with biotinylation of lysines by biotinidase Substitution of arginine with ornithine ... spanning amino acids 1–9 in histone H3; N9)16, peptide spanning amino acids 9–16 in histone H3; N16)23, peptide spanning amino acids 16–23 in histone H3; and N18)25, peptide spanning amino acids...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
... might induce MAP kinase activation independently of ouabain Ouabain was added to the cells at various concentrations in ECGM-SF, and the cells were further incubated at 37 °C for 30 The cells ... were interested in determining whether ouabain added to endothelial cells in culture might induce such a response Various concentrations of ouabain were added to the cell culture wells, and, ... plasma membrane The experiments, however, did not indicate any differences in [3H]ouabain binding to the membrane surface after the cells were preincubated with either or nM [3H]ouabain Whereas cell...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... Fibroblasts deriving from Location C Full lines indicate initial wound area; dotted lines demarcate migrating front of cells GM – CSF treatment of fibroblasts deriving from location A (A) and location ... wound tissue in the OR and HE helped in logging de-identified clinical data and delivering the specimens to MTC MTC supervised OS and SV to carry out the culture the cells in- vitro and perform ... bacterial and fungal contaminants Sterility testing Each culture was tested for mycoplasma using four tests, PCR detection [32], staining using Hoechst dye, culturing for Mycoplasma in broth [33], and...
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báo cáo sinh học:" Continuity and change in human resources policies for health: lessons from Brazil" docx
... attracting and retaining health staff in remote and/ or rural areas, tackling staff mal-distribution and over-specialisation, particularly in the physician workforce, retaining and motivating health ... respond to primary care demands within SUS PROFAE 2002/2007 Aimed at expanding training of nurse technicians and nursing aides PROMED 2003/ Aimed at financing curricular reform in medical schools directed ... training curriculum: there was an increased emphasis on rural internships and the need to provide trained staff in underserved areas In 1977, the first mandatory rural internship was created in...
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Báo cáo sinh học: "Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer" potx
... All human recombinant cathepsins were from Enzo Life Sciences (Plymouth Meeting, PA) Human cathepsins K and S were expressed in insect cells, human cathepsin V was expressed in NSO cells, and human ... Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer Binbin Chen and Manu O Platt* Abstract Background: Cathepsins K, L, and S are cysteine proteases upregulated in cancer and proteolyze ... breast tissue protein was determined by loading increasing doses of recombinant cathepsins K and L in the same gel as the breast cancer and normal specimens to generate a standard curve to which...
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