... unclear In light of the effects of Nef on lipid transport discussed above, the lack of differences in the lipidcomposition in total cell lysates suggest effects of Nef on lipid sorting and/ or microdomain ... the overall lipidcompositionof host cell membranes to optimize virus replication To test this hypothesis, we compared in this study the impact of Nef on the lipidome of HIV-1 virions and T lymphocytes ... regulation by the local lipid microenvironment or causes alterations in the local lipidcomposition [66-68] Finally, the altered lipidcompositionof microdomains in the presence of Nef might have...
... activity was measured according to [81] Lipid analyses Lipids were extracted according to Sommarin and Sandelius [82] and fractionated into neutral lipids, glycolipids and phospholipids by solid ... important property of especially the phospholipids is their charge, with PC and PE being uncharged and PA, PI, PS and PG being negatively charged The charges of the lipid head groups and the membrane ... contributions SW, AR and MA conceived the study and planned the majority of the experiments MA conducted all the experiments HT and ASS took part in the lipid analyses and the interpretation of the results...
... mesocarp, compared to our findings of none detectable The authors also reported 101 ( 38 and 22.1 ( 15.2 g -carotene/g FW in extracted samples of aril and mesocarp, respectively, and 16 and g/g of zeaxanthin ... zeaxanthin and 35 and g/g of -cryptoxanthin in saponified samples of mesocarp and aril tissues, respectively We suggest that the presence of lycopene in gac mesocarp samples was probably a result of ... degree of ripeness andconditionsof culture In addition, our modified extraction procedure was designed specifically to avoid the loss of cis-lycopene isomers, which are of interest because of evidence...
... collapsed phloem is gradual and signalled by the collapse of sieve cells and loss of their radial and tangential alignment, the distortion of rays and the expansion of parenchyma cells These changes ... layers over expanded parenchyma cells The phellem has a small number of thick-walled and small suberised cells The chemical compositionof P considerable amount of tannins and tent of polysaccharides ... to 19,1 % and include mainly polar compounds extracted by ethanol and water (14.0 % of o.d bark); approximately half of the extractives (7.2 % of o.d bark) are of phenolic character and correspond...
... Distribution of synonymous codons along the first and third principal axes of the COA on RSCU values of the genes of 82,927 predicted ORFs of 24 non-halophilic and halophilic chromosomes (c) Position of ... amino acid compositionof real and hypothetical proteomes Average5 Average amino acid compositionof real and hypothetical proteomes Differences between average amino acid compositionof real proteomes ... usage and frequency distribution of genes on hydrophobicity and pI COA on amino acid usage and frequency distribution of genes on hydrophobicity and pI (a) Positions of 24 non-halophiles and halophiles...
... different levels of sugar one hundred mL of nutrient broth (0.25g of yeast extract, 0.25g of malt extract and 0.25g of bacteriological peptone) with different concentrations of glucose (3%, ... other professors of Food Science and Technology (FST) program, such as Prof Zhou Wei Biao, Dr Huang Dejian, Dr Yuk Hyun Gyun and Dr David Popovich, for their teaching and enlightenment to expand ... 95 8.2.2 Sugar and organic acid contents before and after fermentation .98 8.2.3 Ethanol and glycerol contents of mango wine 98 8.2.4 Volatile profile of mango juice and wine 99...
... new knowledge and understanding of the anthropometry and body compositionof Indonesian adults and to develop anthropometric prediction equations to estimate body composition A total of 600 adults ... Bland and Altman plot between the BSQ-16a and BSQ-16b post-test 180 Figure 4.1.6 Bland and Altman plot of the CDRS1 current pre- and post-tests .182 Figure 4.2.1 Bland and Altman plot of ... of FFM (kg) obtained from D2O and BIA equation (Lukaski, 1987) 160 Figure 4.1.1 Bland and Altman plot of the BSQ between pre- and post-tests 176 xiv Figure 4.1.2 Bland and Altman plot of...
... 2.1 Land use land covers change 2.2 The Relation between Land degradation and LULCC 2.2.1 Causes of land degradation 2.2.2 Causes of land cover and Land use changes ... Thesis prepared by Girma Nigussie Asresu, entitled: Dynamics of Land Use and Land Cover Change and Vegetation Compositionof Guassa Community Conservation Area, Amhara Region, Ethiopia and submitted ... Impacts of land use/land cover change 2.4 Remote sensing for land use and land cover detection 11 2.4.1 Remote sensing applications in Land use Land Cover 12 2.4.2 Processing of...
... monitoring parameters of choice under open- chest conditions [4] Only relative changes of SVV, PPV, and GEDVI were able to predict an increase in SVI after sterotomy and volume loading Opening the chest ... relative changes of PPV, SVV, and GEDVI were predictive of volume responsiveness in our study Limitations Our study was designed and performed to estimate the effect of a clinical approach of volume ... 1,364 mL (713 mL) of autologous blood was taken from the patients, and 1,459 mL (605 mL) of HAES 6% and 1,311 mL (742 mL) of crystalloid fluid were substituted as a local standard to account...
... remnants (50– 150 nm [27]), and that the lipidcomposition in terms of the percentage lipid mass of the various lipid classes and TG ⁄ TC molar ratio is also similar to that of physiological remnants ... rates of uptake of CRLPs of different fatty acid compositions by macrophages observed in the present study is due to differences in the oxidative state of the particles Evaluation of the lipid ... when complexed to different lipids [39], and it has been Lipid accumulation in macrophages suggested that the lipidcompositionof lipoproteins can alter the conformation of the protein [40] Thus,...
... helminth lipidbinding proteins of oad their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of ... incorporation of the ligand Binding of DAUDA to Ag-NPA-1 caused 20% quenching of the emission of Trp17 indicating FRET between the single Trp residue and the dansyl group We calculated a value of 3.74 ... ionone ring of retinol would be expected Interestingly, binding of retinol caused an increase of the emission of the single Trp residue, similar to that registered after binding of oleic and arachidonic...
... modes Curve fitting of the infrared spectra of perturbed purple membrane assemblies was performed with a Bomem Grams/386 Briefly, the amide I and II envelope of the infrared spectrum of purple membrane ... I modes of the a-helices), 1680 cm)1 (the amide I of b-turn structures), 1640 cm)1 (the amide I of random coil and b-sheet structures), 1545 cm)1 (the amide II of a-helical A mode), and 1520 ... after depletion of lipids by Triton exposure and subsequent stepwise reconstitution in lipid dispersions containing various concentrations of NaCl which control the extent oflipid rebinding...
... fractionation and Western blotting of HeLa cells were subsequently performed In cells cultured under low lipid concentrations, small amounts of PP17a, PP17b and PP17c were found in the buoyant lipid ... phase of cell cycle, differentiation and apoptosis on expression of this gene was also studied Furthermore, evidence was presented for the association of PP17b to lipid droplets and milk lipid ... Structural relationship of human PP17, adipophilin and perilipin genes and proteins After multiple sequence alignment of PP17b, adipophilin (hADFP) and perilipin (hPLIN), and alignment of their cDNAs...
... , the radical catalyzes reduction of Fe(III) [12] and reductive dehalogenation of recalcitrant aromatic halides [31] O2† – catalyzes oxidation of Mn(II) and reduction of Fe(III) in addition to ... (1991) Isolation and identification of a-(4-pyridyl-1-oxide)N-tert-butylnitrone radical adducts formed by the decomposition of the hydroperoxides of linoleic acid, linolenic acid and arachidonic ... width of 1.0 mm The wavelength range of the spectrometer was 370– 820 nm Experimental conditions are described in the legend of each figure R E S U LT S Formation of glyoxal in the reaction of linoleic...
... Outline Introduction of polyester amide (PEA) What is polyester amide Applications Advantage of Lipid- based components Previous Examples of Lipid- based PEA Objectives and Results Conclusions ... Journal of Biomaterials Science-Polymer Edition, 2007 18(6): p 673-685 Guo, K and C.C Chu, Journal of Polymer Science Part a-Polymer Chemistry, 2005 43(17): p 3932-3944 Advantage of Lipid- based ... Hojabri, L., X.H Kong, and S.S Narine, Biomacromolecules, 2009 10(4): p 884-891 Williams, C.K and M.A Hillmyer, Polymer Reviews, 2008 48(1): p 1-10 Previous Examples of Lipid- based PEA PEA derived...
... S2 and Table S2) The lipid association is required for high affinity binding of apoE to the LDLR because of the increased exposure of basic region on the fourth a-helix after interacting with lipids ... Influence of apoE domain structure and polymorphism on the kinetics of phospholipid vesicle solubilization J Lipid Res 2002, 43:1688 37 Krempler F, Kostner GM, Friedl W, Paulweber B, Bauer H, Sandhofer ... concentrations of GdnCl in PBS (pH 7.3) at 4°C overnight to reach equilibrium The unfolding of the proteins was monitored by measuring the CD signal of 222 nm at 20°C and the width of the cuvette...
... infection of monocytes[5] Together, these studies indicate the importance of HIV and target cell membrane composition for viral function and suggest that a better understanding of the role of different ... components of HIV are also important is less well established The importance of the lipidcompositionof target cells is somewhat better understood Treatment of target cells with sphingomyelinase and ... Layer Chromatography (TLC) and compared these lipid profiles to that of the producer cells from which they were generated Figure shows lipid analysis by TLC of viral and cellular membranes As...
... oxidation and reduction of –SH groups or iron ions These signaling pathways are often referred to as redox regulation, which mediates intracellular signaling (e.g in response of cells to hormones and ... on lowering levels of LDL specifically and also other aspects of dyslipidemia (Libby et al., 2000) The basic role of cholesterol in the promotion of atherosclerosis is now understood Atherogenesis ... Huang and Wang Huansong for their everyday help ii TABLES OF CONTENTS Page Acknowledgements ii Tables of contents iii Abstract viii List of tables x List of figures xii List of abbreviations and...
... circumstances Upon completion of the study, the envelope was opened in the presence of a representative of the sponsor andof the Principal Investigator, and the representative formulas of the samples were ... coloring agent Measurements Body weight and impedance measurements, skin echogram, and waist, hip, and thigh circumferences were measured at the beginning and end of the 30-day treatment phase BMI ... the ultimately randomized participants will be less representative of the general population of patients Because the purpose of this trial was to measure efficacy of the agent under study, we...
... the importance of the shutter region for the unique types of RCL insertion in PAI-1, we have now undertaken a number of substitutions in the shutter region and b strand 5A of PAI-1 and studied their ... conformation of b strand 5A andof the buried cluster in positions 53, 56, and 334 In contrast, in the presence of vitronectin, the K335A substitution caused a twofold to fivefold acceleration of latency ... wild-type, 48.6 and 65.2 min; K335A, 78.9 and 41.8 min; S53A, 69.4 and 109.9 min; S53A/K335A, 99.2 and 43.5 min; G56S/Q334H, 5.3 and 59.8 min; G56S/Q334H/K335A, 26.5 and 35.1 A summary of all experiments...