... soybea1ns contain lower amounts of lysine, which is one of Microbial Production ofAminoAcids in Japan 77 the essential aminoacids for those livestocks The estimated amount of l-lysine produced in ... successful introduction of the technology, various methods were searched for and developed for microbial production of other aminoacids Today a whole array ofaminoacids are produced by microbial ... various amounts of sodium pyruvate, g of ammonium acetate, 0.6 g of pyrocatechol, 0.2 g of sodium sulfite, 0.1 g of EDTA, and cells harvested from 100 ml of broth, in a total volume of 100 ml.The...
... study, to examine the effect of the combination ofaminoacids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the aminoacids at positions and in a model ... the combination ofaminoacids at positions and might be a critical determinant for protein Nmyristoylation In the present study, to examine the effect of the combination ofaminoacids at positions ... addition to the combination ofaminoacids at positions and 6, it was demonstrated that the combination ofaminoacids at positions and also affects the susceptibility of the protein to protein...
... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 aminoacids in antiplasmin A computer model of the C-terminal 40 amino ... absence of 6-aminohexanoic acid) The reactions between ‘native’ human antiplasmin and plasmin in the presence or absence of 6-aminohexanoic acid were also studied for comparison All variants of antiplasmin ... in detail by constructing seven single-site mutants of antiplasmin Fig Computer model of the C-terminal 40 aminoacids in antiplasmin Some of the residues are labelled to facilitate viewing Ó...
... TOWARDS THE SYNTHESIS OF -AMINO ACIDS PAN YUANHANG (BSc., Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE To ... provided the synthetic route towards both -amino acid derivatives and -amino acid derivatives Mechanistically, based on the experimental characterization of intermediates and theoretical calculations, ... reaction of phenylacetate thioesters by soft enolization Scheme 2.3 Cross-aldol reaction of isatins and ketones Scheme 2.4 Synthesis of MAHTs started from diethyl malonate Scheme 2.5 Synthesis of N-sulfonyl...
... M (1961) Chemistry of the Amino Acids, John Wiley & Sons, Inc., New York, London Zavradashvili, N (2008) New functional biodegradable poly(ester amide)s composed of α -amino acids and their potential ... racemization and destruction The use of metalorganic catalyst is one of the drawbacks as well The AABBPs type PEURs can be synthesized on the basis of TAADs under the conditions of either IP or AP similar ... This leads to imbalance of stoichiometry and contributes to the limitation of chain growth In spite of this, Huang’s study initiated a rational synthesis of a large variety of key monomers – TAADs...
... side chain of any bound amino acid The lability of the a-proton observed for a large number ofaminoacids [5] under the action of TPL gives evidence for the orthogonal orientation of the a-proton ... with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety ofaminoacids It has been established [5] that for a number ofamino acid inhibitors ... References Konnikova, A.S., Dobbert, N.N & Braunstein, A.E (1947) Labilization of a-hydrogen ofaminoacids under the action of aminoferase Biokhimia 12, 556–568 (in Russian) Ó FEBS 2004 Esaki, N., Nakayuma,...
... activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 aminoacidsof NBD2B or a stimulatory effect of the equivalent aminoacidsof NBD2A To determine which of these ... last 42 aminoacids Figure 2A and Table show that the ATPase activity of NBD2-DC was greater than that of SUR2B but similar to that of NBD2A, favoring the idea that the last 42 aminoacidsof NBD2B ... equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1...
... involved in the action ofaminoacids on gene expression Amino acid(s) manipulations Factor(s) implied Inhibiting effect resulting from the presence ofaminoacids Pooled aminoacids deprivation Increased ... presence ofamino acid(s) resulted in inhibition of the DNA binding of the involved transcription factors By contrast, the presence ofamino acid may also result in stimulation of the DNA binding of ... effects of different aminoacids (except glutamine), together with the identified transcription factors and the responsive elements involved Most of the data concern the inhibitory effect ofamino acids...
... to consist of six domains: the N-terminal cytoplasmic tail (amino acids 1–18), the helical transmembrane region (amino acids 19–43), and four extracellular domains spanning aminoacids 44–150 ... recovered, apparently as a result of the blocking of the N-terminal amino acid Subsequent Western blot analysis, exploiting the 7E11 mAb recognizing the first six aminoacidsof the full-length GCPII [31], ... removal of as few as 15 aminoacids from the C-terminus completely abolished NAAG-hydrolyzing activity (the 44/736 variant), and the C-terminal extension (addition of the V5-His tag in the case of...
... importance of negatively charged aminoacids for sodium-coupled substrate cotransport or exchange [12,13,16–18] Mutations of all conserved and two nonconserved negatively charged aminoacids to ... mutagenesis of Ntcp (Eur J Biochem 270) 1121 Fig Topology model of rat Ntcp based on hydropathy analysis of the amino acid sequence (according to [1]) Transmembrane domains are symbolized as blocks ofamino ... ofaminoacids Mutated aminoacids are highlighted in gray The resulting mutants are shown in boxes, with deletions indicated (del) Fig Mutations of negatively charged conserved aminoacids alters...
... obtained by exchange of a 1.8-kb HindIII fragment of pSVAR3 with the corresponding fragment of pGAD3D3–37 For construction of pSVAR.NTDD3-37, a 1.7-kb HindIII/Asp718 fragment of pSVAR.NTDwt was ... of three independent experiments Fold induction is shown to the right of each bar and represents the ratio of activities determined in the presence and absence of R1881 (C) Western analysis of ... substitutions ofaminoacids flanking 23FQNLF27 reduced the binding to AR LBD Most prominent inhibitory effects were found for amino acid residues directly flanking 23FQNLF27 Note that expression levels of...
... aromatic aminoacids (Phe, Tyr, and Trp); negatively charged aminoacids (Asp and Glu); positively charged aminoacids (Lys and Arg); small aliphatic aminoacids (Gly and Ala); larger aliphatic amino ... negatively charged amino acids, and instead preferred aliphatic or noncharged aminoacids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining aminoacids facilitates ... side chains of substrates, but not when the negative charge is situated at a C-terminal carboxyl group of a P2¢ amino acid of Fig Analysis of cleavage specificity by the use of new types of recombinant...
... affect the stability of the proteins [28] The C terminus of CpcF shows only little homology for as much as 50 aminoacids A truncation by 53 aminoacids reduced the solubility of the protein, possibly ... modifications ofaminoacids Chemical modifications of arginine, lysine, carboxyl groups, tryptophan and histidine were performed as described before [28] The number of essential residues of a certain amino ... terminus of CpcF showed more homology: a 10 amino acid truncation reduced the activity to 26%, and it was lost completely when 20 aminoacids were truncated Among the 10 N-terminal amino acids, ...
... levels of sorbate or acetate indicated that the loss of Azr1p did not result in increased sensitivity to either of these acids (Fig 1; strains with no auxotrophic requirements for aromatic amino acids) ... aromatic amino acids, might be causing an unusually high sensitivity to weak organic acid stress Auxotrophic requirements for aromatic aminoacids dramatically increase sensitivity to weak organic ... Defects in several of the enzymes of aromatic amino acid biosynthesis therefore hypersensitize yeast to weak organic acid stress We also investigated the growth of this same series of strains after...
... asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc mc se Fig In situ hybridization of the transcripts of GLU1, GLU2 and GLT in Arabidopsis ... mixture consisted of 0.4 ng of genomic DNA isolated from rosette leaves, 10 pmol of forward primer, 10 pmol of reverse primer and 0.2 units of Taq polymerase in a total volume of 25 lL The following ... oxidative deamination (NAD-GDH) of glutamate The activity is expressed as lmol of glutamate formed (GOGATs), lmol of hydroxylamine formed (GS), or lmol of NADH oxidized (or of NAD reduced) (GDH) h)1ặg)1...
... first four aminoacidsof the barley serine racemase and the final 88 aminoacidsof the Pyrobaculum serine racemase are omitted for clarity Alignment was performed using the CLUSTAL software 3540 ... carboxy-terminal end of the polypeptide chain, disrupts its interaction with PDZ4 ⁄ PDZ5 of GRIP, it is tempting to speculate that phosphorylation of Ser336 of human serine racemase or of Thr336 of mouse ... details of the interaction of PICK1 with serine racemase, or on the phosphorylation of serine racemase Considering that the phosphorylation of Ser880 of the GluR2 subunit of the AMPA receptor, positioned...
... Charging of tRNA with non-natural aminoacids M Giel-Pietraszuk and J Barciszewski Table Yield of aminoacylation of tRNA with different aminoacids pmole amino acid per 1600 pmole tRNA Amino acid ... and tRNAVal charging with a series of natural aminoacids showed that the best yields were obtained for aromatic amino acids, but aminoacylation using aminoacids with an aliphatic side chain ... noncognate aminoacids in the aminoacylation of CoA, and in the acylation of mini helix of RNA [32] The equilibrium of CoA acylation was shifted towards an aa-S-CoA formation [33] In the case of high-pressure...
... Preparation of N,N0 -di-Boc -Amino Acids 66 General Procedure for the Preparation of Na-Bpoc -Amino Acids 67 General Procedures for the Preparation ofAmino Acid Methyl Esters 68 Preparation ofAmino ... Procedure for the Preparation of Na-Bsmoc -Amino Acids 64 General Procedure for the Preparation of Na-Aloc -Amino Acids 65 General Procedures for the Preparation of Na-Boc -Amino Acids 65 Method A: Using ... Preparation of Na-Phthaloyl AminoAcids using N-(Ethoxycarbonyl)phthalimide 59 General Procedure for the Preparation of Na-Trt -Amino Acids 59 General Procedure for the Preparation of Na-Ns -Amino Acids...
... β -amino acids including (1) the resolution of racemic β -amino acids, (2) the use of naturally occurring chiral α -amino acids, and (3) asymmetric synthesis (Liu and Sibi 2002) As resolutions of ... application of transaminases for the synthesis of β -amino acidsOf particular interest are methods that can be used at small scale compatible with microtiter plates Enantiopure β -amino acids represent ... variety of aliphatic and aromatic β -amino acids as amino donors with good accuracy Furthermore, by using both enantiomers of an amino donor separately, information on the enantiopreference of the...
... WJ: The stimulus-secretion coupling ofamino acid-induced insulin release: insulinotropic action of br anched-chain aminoacids at physiological concentr ations of glucose and glutamine Eur J Clin ... anched-chain aminoacids prolong exercise dur ing heat stress in men and women Med Sci Sports Exerc 1998, 30:83-91 61 Davis JM, Welsh RS, De Volve KL, Alderson NA: Effects of br anched-chain 33 aminoacids ... Takehana K: Phar macological activities of br anched-chain amino acids: augmentation of albumin synthesis in liver and improvement of glucose metabolism in skeletal muscle Hepatol Res 2004, 30S:19-24...