optimized neuronal differentiation of murine embryonic stem cells role of cell density pdf
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells ... represent averages of two values ND, not detectable (e) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated number of mitomycin...
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differentiation of embryonic stem cells
... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Section I Differentiation of Mouse Embryonic Stem Cells [1] Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive Ectoderm-like (EPL) Cells By JOY...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of ... hES cells and adult cells was also significant (P < 0.05) In particular, the expression of miR-519b was 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note,...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... expression (e) Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls ... BMP2-expressing cells during early differentiation of ES cells will pave the way for a better understanding of NCSCs and their differentiation into lineages The BMP2+ cells derived from the ES cells may ... only 11% of the cells were BMP2+ cells This result demonstrates a nearly 8.5-fold enrichment of BMP2+ cells in EBs treated with puromycin As demonstrated in Figure 1f, plating of the BMP2+ cells...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies to ... stages of embryonic development from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the inner cell mass while epiblast stem cells (EpiSCs) are of epiblast ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... correlated to loss of cell viability upon exposure to low temperature It was observed that some cells detached after exposure to low temperature Virtually all of the detached cells were determined ... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
... assay of pri-miR9-1 during neuronal differentiation of P19 cells Five different upstream regions of pri-miR9-1 were transfected into P19 cells, miR9-1PN3_Fluc produced a strong FLuc signal after neuronal ... expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of pri-miR9-3 neuronal differentiation neuronal differentiation ... resultant mature 23a in HEK293 cells, but not in HeLa cells and P19 cells [19] Highly expressed primiR23a produced a relatively low endogenous level of mature miR23a in HEK293 cells, indicating a slow...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells hES -MCs Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc
... sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... contexts with respect to stem cell differentiation: maintenance of pluripotent stem cells and tumorigenesis of cancer stem cells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB, ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf
... sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... contexts with respect to stem cell differentiation: maintenance of pluripotent stem cells and tumorigenesis of cancer stem cells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB, ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation of embryonic stem cells ... The role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells...
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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx
... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... http://www.jbiomedsci.com/content/17/1/33 Page of 11 Figure The characteristics of the ES cells and their neural derivatives (A) Schematic procedure of SFEB for neural induction of ES cells Undifferentiated ESC 26 cells were characterized...
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Báo cáo y học: "Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells" pot
... Methylation of Cell Cycle Genes in Neural Stem Cells J Neurochem 2010 47 Fujita Y, Hiroyama M, Sanbe A, Yamauchi J, Murase S, Tanoue A: ETOH inhibits embryonic neural stem/ precursor cell proliferation ... investigate inhibitory effect of ethanol on cell proliferation, immunocytochemistry against pH3 or PCNA was performed on day The number of pH3-positive cells as well as PCNA positive cells was reduced by ... proliferation of NPCs in the early stage of neurogenesis In immunochemical staining, the number of nestin positive cells was decreased by ethanol treatment while Tuj-1 positive cells showed increased...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... depletion/reoxygenation conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... Journal of Biomedical Science 2011, 18:46 http://www.jbiomedsci.com/content/18/1/46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonic stem cells A: top view of a single ... cytosol isoforms of MsrA which are 1920kD in size [10,13] To confirm the existence of this smaller form of protein, we have carried out RT-PCR using total RNA extracted from mouse embryonic stem cells...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... in pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous ... pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types ... reconstruction of larger skeletal elements Through the ability of embryonic stem cells to differentiate along the whole osteogenic pathway, embryonic stem cell transplantation may play a future role in ... totipotent embryonic stem cells cultured in three-dimensional micromass technique in the absence of any scaffold The osteogenic differentiation of the cells was induced by the addition of dexamethasone,...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic ... mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation ... in murine embryonic stem cells Biochem Biophys Res Commun 2001, 284(2):478-484 Yamashita A, Takada T, Narita J, Yamamoto G, Torii R: Osteoblastic differentiation of monkey embryonic stem cells...
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