ok but what really happens to m· when it is propelled into the center of the quadrupole
Báo cáo y học: " GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease" pot
... with the salineinoculated group The groups of BoIL-2/BoGM-CSF/ VP1 and BoIL-2/VP1+BoGM-CSF/VP1 showed the highest level of mRNAs of either Th1 or Th2 cytokines Expression of the cytokines in the ... use of two cytokines had better effect than that of single cytokine as adjuvant It suggested that these cytokines enhanced the cell-mediated immunity, which was consistent with their known biological ... groups with single cytokine co-expressed or mixed with VP1 showed the same level of either Th1 or Th2 cytokines as that of groups of Vaccine and VP1 The results indicated that Figure Semi-quantitative...
- 10
- 301
- 0
Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... because it is the natural ligand for CXCR4 which is used by HIV Ttropic strains to enter into the cells in advanced stages of the disease [10] Additionally, although the interpretation of SDF-1 ... non-parametric tests when needed and logistic regression to test the association of gene expression with the use of fenofibrate Results and Discussion There were no significant differences among the three ... (0.04 to 1.1); P = 0.07] Therefore, our results show less lipid disturbances with the addition of fenofibrate in patients with HIV, which is accompanied by significant changes in the pattern of...
- 4
- 375
- 0
Báo cáo khoa học: Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina BBS pot
... Some of the pleiotropic accessory proteins (Hyp) are required for the proper assembly of the H2-activating [NiFe] site in RH [5] The catalytic activity of RH is low, but the activity is insensitive ... from the RH to the histidine kinase in vivo inhibits phosphorylation of the response regulator Therefore the DNA-binding positive regulator remains unphosphorylated and binds to its target site ... (2004) Bacteriophytochrome and regulation of the synthesis of the photosynthetic apparatus in Rhodopseudomonas palustris: pitfalls of using laboratory strains Photochem Photobiol Sci 3, 587–591...
- 10
- 551
- 0
expression of recombinant genes in eukaryotic systems
... order to silence the endogenous copies of the dhfr gene Also, the maximal degree of amplification achievable with respect to copy number will be lower due to the insensitivity of the mutant dhfr toward ... ClS-REGULATOR¥ DNA ELEMENTS 45 Because of the limitations of these approaches, the most definitive way to assess the function of cis-regulatory elements may be to mutate them at their endogenous loci by homologous ... variety of cell lines successfully; the rest of the 172-kb viral genome is dispensable for the extrachromosomal replication of plasmids: In the absence of selective pressure, the episomal entity is...
- 405
- 349
- 0
Báo cáo sinh học: "Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" potx
... minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this case) to the ... it has become apparent that limiting MOI is desirable in order to minimize the risk of insertional mutagenesis [19-21] Therefore, in order to determine whether the use of multi-cistronic vectors ... cells (Figures and 8) Taken together, these data demonstrate the ability of tricistronic vectors to permit the simultaneous expression of three transgenes, albeit with substantial differences...
- 16
- 535
- 0
báo cáo hóa học:" Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" ppt
... minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this case) to the ... it has become apparent that limiting MOI is desirable in order to minimize the risk of insertional mutagenesis [19-21] Therefore, in order to determine whether the use of multi-cistronic vectors ... cells (Figures and 8) Taken together, these data demonstrate the ability of tricistronic vectors to permit the simultaneous expression of three transgenes, albeit with substantial differences...
- 16
- 200
- 0
Báo cáo y học: " Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence" pps
... [19] Cytokines are important mediators and regulators of both types of host responses However, little is known about the role of cytokine following IBDV infection It is extremely important to gain ... cytokine expression were closely associated with virulence of the virus and viral replication Further studies are necessary to elucidate the function of the cytokines in pathogenesis and immunity ... to test the hypothesis that the IBDVs with varying virulence induces different cytokine profiles during the course of infection Results Generation of standard curves for real-time PCR analysis...
- 9
- 387
- 0
Differential expression of cellular genes during a west nile virus infection
... dimensionality A 10 array experiment with 15,000 genes will constitute a matrix of 10 x 15,000 To facilitate a visual analysis of the data, a reduction of the dimensionality of the matrix is necessary ... each element of the array, and the logarithm of the ratio of Cy5 intensity to Cy3 intensity is calculated Positive log (Cy5/Cy3) ratios indicate relative excess of the transcript in the Cy5-labelled ... (Catteau et al., 2003) Since the introduction of WNV C protein into the nuclei of host cells has been shown to induce apoptosis, it could contribute to the pathogenesis of flavivirus infection (Yang...
- 209
- 215
- 0
Expression of EBV genes in nasopharyngeal carcinoma
... (NPC) is a malignancy of the stratified squamous epithelium of nasopharynx NPC is rare in most countries, but it has a high incidence South-East Asia NPC is distinguished from other cancers of the ... during mitosis and is a key mediator of EBV DNA binding to chromosomes EBNA1 also has some function in regulation of gene expression For example, it can interact with two sites downstream of Qp to ... Epstein-Barr virus into latently-infected B cells leads to induction of the entire lytic cycle program of the virus (Takada et al., 1986) Rta is also shown to be able to disrupt latency in epithelial cells...
- 136
- 614
- 0
Báo cáo hóa học: "A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw" docx
... carried out with a WIS content of 7% with a total working weight of 1.4 kg To obtain the initially desired WIS content in the bioreactor, the pretreated, undetoxified slurry was diluted with sterile ... hydrolysis is 1.11 times the mass of glucan (due to the addition of water) For xylose the corresponding number is 1.13 times the mass of xylan Results The current study aimed to evaluate the relative ... product formation from xylitol to ethanol The current comparison using isogenic S cerevisiae CEN.PK strains was undertaken to elucidate the relative contribution of these two beneficial genetic...
- 8
- 329
- 0
Báo cáo sinh học: "Co-regulation of mouse genes predicts function" pps
... did the experiments take and what were the steps that ensured success? It took about two years: one to get the data and another year to the analysis We did several things differently from other ... Chang, to dissect mice over the summer to obtain the tissues we were still missing Our bioinformatics collaborators, Brendan Frey and his postdoc Quaid Morris, were also indispensable; without them ... analyses of mammalian gene expression [4-6] Volume 3, Article 19 Weitzman 19.3 But what I like about this paper is that it s really rock solid,” says Stuart Kim of the Stanford University Medical Center, ...
- 5
- 277
- 0
Báo cáo lâm nghiệp: "doubling atmospheric CO concentration on 2 matter distribution and CO exchange of 2 yr growth, dry 2 old sweet chestnut trees (Castanea sativa Mill.)" pptx
... period over which the main branches grew After the end of July, 62% of the enriched plants ceased growth as compared with 37% of the controls The early onset of yellowing and the stopped terminal ... shoot growth, which is very unusual and may have resulted from the timing of the nutrient application The accumulation of the carbon surplus was restricted to the root system These changes could ... indicates earlier senescence of the -enriched C0 plants The final leaf area per plant, at the end of the growth period, was reduced by C0 enrichment The pattern of growth through the vegetative development...
- 3
- 237
- 0
Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... resolve the synteny in the uncovered and/or the scattered genome regions Rearrangement of the B rapa genome Comparison of the genomes of Br and At allows insight into the origin and evolution of the ... The vertical axes indicate the frequency of paired sequences, while the horizontal axes denote Ks values with an interval of 0.1 The black bars depict the positions of the modes of Ks distributions ... However, the estimated total number of genes in the Br genome is only twice that of At Details of the annotation are available online at the URL cited in the 'Data used in this study' section in the...
- 18
- 487
- 0
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... contributions PD carried out the experimental work and the majority of analysis, prepared the tables and figures and the primary drafts of the manuscript, and contributed to finalisation of the ... (Magnaporthe grisea), dollar spot (Sclerotinia homoeocarpa) and brown patch (Rhizoctonia solani) reduce turf quality [9] The development of cultivars resistant to each of these diseases is currently ... interactions, similar qualitative and quantitative genetic mechanisms are likely to contribute to disease resistance in perennial ryegrass In order to test this hypothesis, a broad survey based on empirical...
- 22
- 446
- 0
báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... development initiative The authors also wish to thank the Province of British Columbia and the BC wine industry for their continued support of research activities in the University of British Columbia ... seeds when cellular activity has diminished and the water content in the pericarp has peaked Yields were quite diverse depending on the source of the tissue Leaves yielded the highest amount of total ... veraison seed; 8, post-veraison seed; 9, root; 10, pre-anthesis flower; 11, post-anthesis flower The fluorescence plot shown in the upper right corner is an example of the electropherogram with...
- 11
- 376
- 0
Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... extraction with Triton X-114 44 2.7 Overview of the mechanism of GST pull down 48 3.1 Interactions profiles of GST-CRP-2 62 3.2 Interactions profiles of GST-CRP-1 64 3.3 Densitometric analysis of CRP-1 ... cascades The complexity of the pathogenesis of septicemia remains the greatest obstacle to its prevention and treatment 14 FIG 1.2: The consequence of sepsis include over production of cytokines ... induction of the inflammatory network When production of pro-inflammatory molecules is pushed beyond physiologically tolerable levels, the balance of cytokine-induced inflammatory responses is tipped...
- 129
- 467
- 0
Báo cáo y học: "otifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons" ppsx
... phenol-chloroform procedures, precipitated with ethanol, and dissolved in 10 mM Tris/10 mM EDTA PCR was performed to determine the presence of the EGFP gene Immunohistochemistry of transgenic positive ... (Table S4 in Additional data file 1) upon the modified BAC and then cloned into the EcoRV site of the pBSKS+ vector Resultant colonies were screened by the presence of EGFP and positive clones were ... neuronal tissues Unbiased in vivo and in vitro screens of cis-regulatory sites for Rab3A, one of the nine genes, revealed regulatory roles for all MCEs in the vicinity of Volume 10, Issue 7, Article...
- 18
- 320
- 0
Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... insert) of the anterior part of the floor-plate (fp) positioned at the basis of the commissure separating the two lobes of the mesencephalic tegmentum (t) and above the hypothalamus (hy) (A–E) The ... cells of the anterior part of the floor-plate were positioned at the base of the commissure separating the two lobes of the mesencephalic tegmentum and above the hypothalamus (Fig 4A–E) Situated ... its tridimensional conformation [2] Thus, it is important to compare these parameters in the different vertebrate species to evaluate the risk of a prion passage from one species to another The...
- 14
- 547
- 0
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability of the ... also shows similarity of the torafugu IL-10 sequence to its carp counterpart These comparisons suggest that carp IL-10 shares many characteristics of IL-10 with its family members The general IL-10 ... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The...
- 8
- 584
- 0
Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... liver, the chicken ectoATPDase is also present in the apical membranes of the oxyntic-peptic cells [37] The distribution of the ectoATPDase on these epithelial cells is distinctly different from the ... inhibition by azide was of the mixed and uncompetitive type [31] The mechanism of inhibition of fluoride and vanadate has not been investigated, but they are unlikely to be competitive inhibitors ... Unlike some other E-ATPases that exhibit similar or higher ATP hydrolysis activity in the presence of Ca2+ [26], the CaATPase activity of the purified chicken liver ecto-ATPDase is 30% of the MgATPase...
- 10
- 694
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008