novel analytical approach to study mammalian glycerophospholipids and sphingolipids
Novel lipidomic approaches to analyse glycerophospholipids and sphingolipids in complex mixtures using mass spectrometry
... Motivations and aims 30 Chapter Novel Analytical Approach to Study Mammalian Glycerophospholipids and Sphingolipids 37 2.1 Introduction 38 2.2 Materials and ... compatibility of sterols and sphingolipids and evolution 108 4.4.6 Lipids and sensitivity to drugs 109 Chapter High Resolution and Targeted Profiling of Glycerophospholipids and Sphingolipids ... Non-targeted profiling and characterization of glycerophospholipids and sphingolipids of slc1Δ by ESI-MS, MS/MS and MS3 67 3.3.3.2 Non-targeted profiling of glycerophospholipids and sphingolipids...
- 225
- 1,280
- 0
Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt
... Iacopetta et al cytosol for the activity of transketolase, and in the mitochondria for the activity of pyruvate-, oxoglutarate- and branched chain keto acid dehydrogenases To our knowledge, in ... the expression vector, without (lanes and 3) and with the coding sequence for DmTpc1p (lanes and 4) Samples were taken at the time of induction (lanes and 2) and h later (lanes and 4) The same ... sulfonate and bathophenanthroline (strong inhibitors of several mitochondrial carriers) and, to a lesser extent, by pyridoxal 5¢-phosphate, mersalyl and mercuric chloride (other strong inhibitors...
- 10
- 300
- 0
Báo cáo sinh học: " Imaging with isotopes: high resolution and quantitation" potx
... Slodzian’s group and my experience in tracers and micromanipulation We had to learn how to play with the samples, how to the experiments with tracers and the calculations, etc And the software ... its real beauty is to be able to precise quantitation Suddenly, we have the ability to see and measure things that we could not see or measure before How long did the study take and what were the ... also doing experiments where we take thousands of images of a single cell, slowly ‘shaving’ off layers from the top to the bottom, and then reconstruct them to create a three-dimensional representation...
- 5
- 231
- 0
Adiponectin in cattle profiling of molecular weight patterns in different body fluids at different physiological states and assessment of adiponectin’s effects on lymphocytes
... of several proand anti-inflammatory cytokines Its main anti-inflammatory function is potentially related to its capacity to suppress the synthesis of tumor-necrosis factor α (TNFα) and interferon-γ ... applied to the protein samples to linearize proteins and to dissociate them from each other SDS binds non-covalently to proteins to impart a negative charge so that all proteins are directed to the ... withers and sternum) and three vc depots (omental, mesenteric and retroperitoneal), as well as the mammary gland, was sampled Tissues were immediately frozen in liquid nitrogen and stored at...
- 131
- 350
- 0
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... four active site residues of Dm-dNK Residue E52 was mutated to D, H and Q, residue Y70 to W, residue Q81 to N and residue R105 to K and H The kinetic properties of the active site mutants are ... nucleoside, converting the pro-drug to the active cytotoxic drug The nucleoside analogue can be incorporated into the DNA chain and cause chain termination, induce apoptosis or inhibit DNA polymerase ... histidine can act as both a proton donor and an acceptor in enzymatic reactions, and it should therefore theoretically be able to replace glutamate as a base, to some extent However, this is...
- 10
- 504
- 0
Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc
... positioned to form specific interactions to the sugar and the base of the substrate The 3¢-oxygen of deoxyribose is hydrogen bonded to Tyr70 and Glu172, and the 5¢-oxygen is hydrogen bonded to Glu52 and ... inhibitor was indeed bound as a bisubstrate inhibitor occupying both the phosphate donor and acceptor sites Here, a magne2156 sium ion was bound to the phosphates [13] The binding of the inhibitor ... been determined in complexes with substrates and a feedback inhibitor [12,13] It has a structure similar to that of the human dGK and dCK and belongs to a structural family that also contains some...
- 10
- 308
- 0
South Africa''''s role in conflict resolution and peacemaking in Africa docx
... perpetrators enabled to tell their stories; and, importantly, it directed its attentions to both pro- and anti-apartheid elements, to the formerly ruling NP and its cohorts and to the ANC It is against ... policy’ (Mandela 1993: 93) Both the Mandela and Mbeki administrations drew a one -to- one connection between lack of human rights and democracy, on the one hand, and Africa’s instability and lack ... backed by Uganda and Rwanda invaded the eastern part of the country and headed towards Kinshasa to remove Kabila from power When Kabila cried to the SADC for help, Angola, Namibia and Zimbabwe...
- 280
- 506
- 0
Báo cáo hóa học: "Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea" ppt
... M: Histochemical, microchemical (microprobe) and organ culture approaches to the study of auditory development Acta Otolaryngol Suppl 1985, 421:10-18 49 Pujol R: Morphology, synaptology and electrophysiology ... support and hair cells Arq Int Otorrinolaringol 2007, 11:433-437 16 Albuquerque AA, Rossato M, Oliveira JA, Hyppolito MA: Understanding the anatomy of ears from guinea pigs and rats and its use ... cochlea and vestibular system[12,13], and adult human and guinea pig spiral ganglion[27]; (2) spheres were shown to be clonal and capable of self renewal[12,13]; and (3) spheres were able to differentiate...
- 10
- 476
- 1
báo cáo khoa học: " Variation at four enzyme loci in natural populations Drosophila melanogaster : factor analyses of genotypic and gametic associations Angeles ALONSO-MORAGA" pdf
... directional and random pressures In order to detect these two factors we use multivariate analyses (factor analysis or analysis of principal components), with the objective of measuring the factors ... to produce de novo variation, together with recombination (L 1974) Random fluctuation of gene , EWONTIN frequencies, due to the random sampling of gametes, leads to fixation or loss of alleles ... which correspond to each of the samples in the four populations, and by the variables (columns) which are shown in figure to respectively (3 at Factor analysis is well known, and the idea behind...
- 10
- 229
- 0
evolution of credit and banks in france, from the founding of the bank of france to the present time
... They wrote to their correspondents in the provinces that they were obliged to stop business and go into liquidation This decision increased the panic in Paris, and caused it to spread to the departments, ... (I) The Comptoir of the Book Trade; (2) the Comptoir of Metals; (3) the Comptoir of Builders and Contractors; (4) Comptoir of Colonial Commodities; ( ) Comptoir of Dry Goods; (6) Comptoir of Textiles; ... France took the standard method; it raised the discount rate At the beginning of October it raised the rate to per cent; on October 13 it raised it to 6.5 per cent; on October I it raised it to 7.5...
- 136
- 1,041
- 0
báo cáo khoa học: "Genomic profiling of plastid DNA variation in the Mediterranean olive tree" pdf
... genomes of ‘Frantoio’ (GenBank/EMBL accession GU931818; Italy; [18]) and ‘Manzanilla de Sevilla’ (Spain; this study) According to our approach, we re-sequenced the variable regions in ‘Frantoio’, from ... experiments and wrote the initial version of the manuscript GD and PH contributed to olive cpDNA sequencing and to the acquisition of cultivated olive genotyping data BK contributed to the Besnard ... europaea) and Ligustroides (O woodiana) is estimated to be between 14 and 22 million years (My; [29]) Based on these results, the cpDNA substitution rate was estimated to be between 1.2 × 10-10 and...
- 12
- 581
- 0
Báo cáo y học: " Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation" ppsx
... eosinophils, and macrophages [5] Macrophages are the predominant immune effector in the alveolar spaces and airway, and are believed to play a pivotal role in various pulmonary inflammatory disorders ... also produce inflammatory mediators, such as platelet-activating factor, interleukin beta (IL1β), IL-6, IL-8, and tumor necrosis factor- alpha (TNF-α) [11-14] These mediators may play important ... regulators (n = 9), such as transforming growth β-Induced factor and Down syndrome critical region protein 1; (4) transport regulators (n = 7), such as CD36 and transcobalamin II; (5) cytokines...
- 12
- 237
- 0
Báo cáo khoa học: Regulation of calpain B from Drosophila melanogaster by phosphorylation pot
... controlling various cellular processes, such as cytoskeletal remodeling, cell cycle, apoptosis and cell motility [1,2] The ubiquitous mammalian l- and m-calpains (calpain-1: EC 3.4.22.52; calpain-2: ... (control) was monitored by SDS ⁄ PAGE The arrow points towards the calpain B bands The densities of the intact MAP2c bands (indicated by an arrowhead) were estimated by densitometry and the apparent ... fluoride, 10 mm b-mercaptoethanol and 10 mm NaF The column was washed repeatedly with 100 lL aliquots of this buffer, and the first and the second fractions were pooled and stored on ice until activity...
- 14
- 314
- 0
Báo cáo khoa học: Characterization of a functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster doc
... suspended in 200 lL Hepes buffer and stored at )20 °C A total of L of stable transfected S2 cells induced for 48 h were thus prepared for chromatography and the total cytosolic sample contained Ó FEBS ... standard solutions of di- and tripeptide The effect of proctolin concentrations (from to 250 lM) and the effect of tynorphin concentrations (from 100 lM to 0.1 nM) on the degradation of proctolin ... B expression vector Arrows represent the primer pairs (1 and 2; and 4) used in the PCR and RT-PCR experiments, and the approximative sequence area expected to be amplified (1 kb and 0.8 kb, respectively)...
- 9
- 357
- 0
Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
... al TGAG-3¢; and reverse, 5¢-CTAGAAATCGGGGTCATT CA-3¢ The amplified cDNA was cloned into the pGEM-T vector (Promega, Madison, WI) To identify the 5¢- and 3¢-termini of the gene, 5¢- and 3¢-RLM-RACE ... 2-mercaptoethanol, mm phenylmethanesulfonyl fluoride, mm leupeptin, mm pepstatin A, 0.5% Triton X-100) at °C for and centrifuged at 3000 g for (MX-201; TOMY; TMA-29 rotor) The soluble cytoplasmic ... endonuclease (FEN-1), and XP-G, and also a C-terminal tail that interacts with DNA polymerase e and replication factor C [3] These regions are conserved in both DmPCNA2 and DmPCNA1, but a small...
- 12
- 404
- 0
Báo cáo y học: "Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients" pptx
... chondrocytes are known to upregulate the production of their own pro-inflammatory cytokines, including IL-1β and tumor necrosis factor-α, under the influence of proinflammatory cytokines present in ... and II collagen (CI and CII, respectively), aggrecan (AGG), IL-1β and activin-like kinase (ALK)-1 was determined using qRT-PCR The mRNA levels were normalized to GAPDH and amplified by a factor ... IL-1β and activin-like kinase (ALK)-1 as indicated The mRNA levels were normalized to GAPDH and amplified by a factor of 106 The ratio of type I to type II collagen mRNA levels continue to increase...
- 10
- 523
- 0
Báo cáo y học: " Protocol for investigating genetic determinants of posttraumatic stress disorder in women from the Nurses'''' Health Study II" pot
... this study will be the largest PTSD candidate gene study conducted to date and the only study in an all-female sample Cases and controls will be carefully matched in terms of trauma history and, ... background and design of the study and drafted the manuscript ID, SMP, JRE, JWS, and RJW contributed to the background and design of the study SMP developed the statistical approach and conducted ... comparison, and also at the experiment-wide level The standard SNP-by-SNP approach yields powers of 24% and 6% respectively, whereas the new approach gave 70% and 58% power This novel approach is...
- 20
- 326
- 0
Báo cáo y học: " Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing " potx
... (Integrins, LFA) and talin, which are known to link adhesion rings to the actin cytoskeleton, as well as to cause polarization of the microtubule organization center (MTOC) toward the synapse ... localize ZAM particles to the MTOC, allowing particles to exploit the microtubule network and be transferred from the posterior pole of the oocyte to the anterior one close to the germ cell nucleus ... resins and allowed to polymerize under a UV lamp at 4°C for days Sections were obtained with an LKB ultramicrotome and mounted over uncoated nickel grids To detect viral antigens by gold immunocytochemistry,...
- 9
- 188
- 0
A discourse analysis of urban water management in singapore from 1960 2009
... society come to engage and understand (Dryfus and Rabinow 1984) In this sense, discourse analysis seeks to engage and study the discourses to uncover the formative relations behind them, and to explain ... important when engaging with texts and language within discourse analysis to be able to understand and relate to the necessary context in order to further understand the discourses (Potter 2004) ... claiming to be a strategy to “rebuild and 41 “A tribute to the board” 10 August 1969 Straits Times The HDB amended the Land Acquisition Act in 1966 and this allowed the government to “acquire any land...
- 156
- 603
- 0
Consuming more than just water a discourse analysis of urban water management in singapore from 1960 2009
... society come to engage and understand (Dryfus and Rabinow 1984) In this sense, discourse analysis seeks to engage and study the discourses to uncover the formative relations behind them, and to explain ... important when engaging with texts and language within discourse analysis to be able to understand and relate to the necessary context in order to further understand the discourses (Potter 2004) ... claiming to be a strategy to “rebuild and 41 “A tribute to the board” 10 August 1969 Straits Times The HDB amended the Land Acquisition Act in 1966 and this allowed the government to “acquire any land...
- 156
- 669
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25