nitrogen fixation in the clostridia
Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
... This is exactly the result we find The authors of that study further found that the hyaluronanbinding (G1) domain of aggrecan is retained early in the disease process and then finally released ... further analysis Determination of protein content Total protein was determined using the commercially available BCA™ Protein Assay Kit in accordance with the instructions of the manufacturer (Pierce, ... levels will be independent of the confounding factor of increased synthesis, which can occur during active repair In summary, we have demonstrated for the first time that (a) proteins in the deeper...
- 9
- 263
- 0
Post translational regulation of the human TRIP br1 cell cycle protein
... TRIP-Br1 interacts with DP1 and other proteins in vivo 5.1 TRIP-Br1 interacts with DP1 in the E2F1/DP1 transcriptional complex in vivo 5.2 The DP1-binding region of TRIP-Br1 54 5.3 The TRIP-Br1 binding ... Functional domains of the MDM2 protein including the p53 binding domain, nuclear localization sequence (NLS), nuclear export sequence (NES), acidic domain, zinc finger, RING finger, and nucleolar ... regulated Unable to induce S-phase in quiescence cells Mainly involved in the regulation of differentiation and development • Lacks the domains involved in transactivation and binding to the pRB family...
- 142
- 350
- 0
Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... corresponding to the constructs encoding the 88 kDa protein and virtually none encoding the message for the 74 kDa protein (Fig 2A) Remarkably, a dramatic increase in the mRNA content in cells ... which regulates the flow of the Gibbs free energy inherent in C–C bonds in fatty acids into the production of either chemical energy or heat by directing the flow of iPLA2c into either the peroxisomal ... regulatory protein binds to the Inr region in the pre-exon sequence (Fig 7C) Finally, we noted the presence of a sequence corresponding to three E-boxes with the sequence 5¢-CAGGTG-3¢ within the 350...
- 16
- 438
- 0
Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... located at the A surface passing through the hole on the other side of the helix and the other at the D surface passing through the C surface on the other side of the helix aspartic acid residues ... reconstruction of the stain envelope The A surface clearly corresponds to the dimer interface conserved in all the homologues Docking of the model [21] (see Experimental procedures) into the stain envelope ... optimal fit to the stain envelope Four important insights emerge from the docking (Fig 7) The C-terminal region is located on the inside of the helix adjacent to the central channel There is some...
- 10
- 450
- 0
Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
... evaluated in platelets through the ability of the activated RAS–GTP to bind a GST fusion protein containing the RAS-binding domain of RAF Different platelet agonists including thrombin, the snake ... bind to its components For example, the RAF kinase inhibitor protein can bind RAF and MEK and thereby prevent their interaction [28,29] A reduction of the expression of the scaffolding proteins ... precipitated using GST fusion protein containing the RAS-binding domain of RAF Precipitated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top) Proteins...
- 7
- 436
- 0
Báo cáo khoa học: Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains pot
... Histidine binding at the FIH active site apparently induces a stacking interaction between the substrate imidazole and the phenolic rings of Tyr 102FIH and His 199FIH, which is one of the iron-complexing ... with their 293T transfected counterparts, the data from the two expression systems are in good agreement Indeed, we have found that, in these cases, the level of hydroxylation in protein obtained ... hydroxylation to that corresponding to His 553 in the tankyrase-2 ARD (Fig S1) Having established that His-containing peptides are FIH substrates in vitro, we then investigated whether tankyrase-2 might...
- 12
- 198
- 0
Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... nutrients activate TOR signaling, which then results in increased translation following activation of the protein kinase S6K and the translation initiation factor eIFE4 and inhibition of mitochondrial ... long-lived The increase in branched-chain amino acids in long-lived worms is intriguing for two reasons First, it opens the possibility that protein meta boism plays an important role in life-span ... and the insulin and TOR pathways in C. elegans longevity Black lines indicate the activating or repressive function of members of different signal transduction pathways Amino-acid pools and other...
- 3
- 389
- 0
Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
... 24 or 48 hours of incubation (data not shown), indicating that TNF-α induced CCL18 mRNA expression in the absence of protein synthesis Interestingly, as shown in Figure 4a, neither blood PMN from ... are the predominant source of TNF-α in co-cultures The results therefore suggest that cell contact with endothelial cells induces TNF-α in PMN, which in turn induces CCL18 production by PMN in ... in SF PMN, thereby confirming the results of the microarray analysis These data therefore indicate that chemokine responsiveness of SF PMN is regulated, at least in part, through the differential...
- 12
- 394
- 0
Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
... studies investigating innervation in the degenerate IVD have also demonstrated expression of Trk-A, the highaffinity receptor for nerve growth factor (NGF), on ingrowing nerves – indicating that ... regulate inflammatory pain in sensory nociceptive neurons in part through the expression of BDNF [33] A relationship between NGF and BDNF within the IVD therefore requires futher investigation The ... IL-1β in the stimulation of NGF from fibroblasts [36], and therefore whether the effects of TNFα on neurotrophin expression in the IVD also requires the presence of IL-1β remains to be investigated...
- 9
- 436
- 0
Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
... Triflavin inhibits platelet aggregation through direct interference with fibrinogen binding to the αIIbβ3 integrin [10] There is now a multitude of evidence suggesting that the binding of fibrinogen ... to the αIIbβ3 integrin is the final common pathway for agonistinduced platelet aggregation Therefore, we further evaluated whether or not simvastatin directly binds to the platelet αIIbβ3 integrin, ... Simvastatin (30 and 50 μM) did not significantly affect FITC-triflavin binding to the αIIbβ3 integrin in platelet suspensions (c, 4.8 ± 0.1; d, 4.9 ± 0.1) (Fig 1C), indicating that the inhibitory...
- 10
- 315
- 0
Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
... signaling pathway are also involved in the positive control of VEGF expression However, the exact role for STC-1 in inducing both tumorigeneisis and angiogenesis in cancer is not well understood In ... (Figure 2A) Then we considered whether the culture supernatants could regulate the migration of HUVEC The Millicell cell culture insert was used to study the migration of the HUVEC in vitro The migration ... by dissolving in DMSO and stored at -20°C Before labeling, cells were washed and re-suspended in PBS containing 0.1% BSA (PBS/BSA) CFSE was then added into the cell suspensions at a final concentration...
- 9
- 404
- 0
Báo cáo y học: " Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a" pdf
... is involved in recruiting Suv39h1 and G9a, but also other proteins, to the histone tails For example, the chromatin remodeling complex WINAC has been described to bind H3K14ac via WSTF to induce ... performed the MALDI-TOF analysis to confirm the H4K16ac modification and helped in the analysis of MS data All the authors have participated in discussing the results and reading the manuscript All the ... we were interested in the 'histone code' associated with the HMTs Suv39h1 and G9a, as these two HMTs generally localize to two distinct regions in the nucleus Studying modifications of the histones...
- 10
- 336
- 0
Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
... fractionation is done by cross-linking chromatin to DNA in vivo and then separating the aqueous phase from the organic phase in phenol:chloroform extractions Free DNA segregates into the aqueous phase and ... for the change in nucleosome occupancy in a typical yeast gene in different transcriptional states (a) When there is no transcription, repressor proteins bind to their DNA-binding sites and maintain ... should be taken into account when interpreting genome-wide experiments involving post-translationally modified nucleosomes When examining the distribution of modified histones in the genome, one...
- 3
- 253
- 0
Báo cáo y học: "Translational regulation of gene expression" docx
... (CPEs) in the 3Ј ends of transcripts regulated in this manner In Xenopus, phosphorylation is mediated by the protein kinases Eg2 (known as Aurora A in the mouse) and Cdc2 The role of the CPEs in the ... kinases (ERKs) induces repression of translation In contrast, tyrosine phosphorylation of ZBP1 by Src kinases inhibits its binding to RNA, thus antagonizing the repression In addition to being ... reinitiate translation upstream or downstream These results indicate that aberrant termination is linked to NMD Jacobson also showed that tethering the poly(A)-binding protein Pab1p or its interacting...
- 3
- 237
- 1
REGULATION OF CHEMICALS IN EUROPE – REACH
... man exposed indirectly via the environment RECOMMENDATIONS i There is a need for further information and/or testing ii There is at present no need for further information and/or testing and for ... with human intake • Estimation of human exposure • Calculation of an MOE 36 MARGIN OF EXPOSURE (MOE) Definition: The ratio between the defined point on the dose-response curve for the adverse ... competitiveness of the EU chemical industry Prevention of fragmentation of the internal market Increased transparency Integration with international efforts Promotion of non-animal testing 10 HOW...
- 39
- 234
- 0
Understanding the regulation of cytokinesis in fission yeast
... phenocopied sin mutants in that the actomyosin rings were not maintained upon completion of mitosis Examination of SIN proteins in the temperature-sensitive mutant nuc2-663 showed that SIN signaling was ... is the primary motor protein responsible for cytokinesis The contractile rings of animal cells are composed of several actin binding proteins such as alpha-actinin, tropomyosin, anillin, filamin, ... al., 1994) These proteins participate in cross-linking and/or stabilization of F-actin, and anchor the actomyosin ring to plasma membrane Together, they organize F-actin and myosin II into a dynamic...
- 165
- 98
- 0
Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
... In addition, myelin also contains some glycoproteins, which are mainly involved in the maintenance of myelin and axon-myelin interaction The examples for the myelin-associated glycoproteins include ... F-actin bundling proteins contain two F-actin binding domains in their sequences or could form multimeric proteins if only one F-actin binding domain is present in their sequences (Winder et al., ... membrane-binding domain, followed by an extended -helical domain and a C-terminal actin-binding domain The identity of amino acid sequence with ezrin in each domain is indicated As shown in Fig 1.4, the...
- 166
- 227
- 0
Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
... of the spot prevent actomyosin ring assembly, implying that the spots may be the precursor of the astomyosin ring (Wong et al 2002) Prior to the formation of the actomyosin ring many of the ring ... ring producing two daughter cells of equal size The actomyosin ring consists of about 50 different proteins including actin, actin regulatory proteins, type II myosin heavy and light chains The ... However, they appear to be maintained in a cytokinesis competent state, despite having interphase nuclei, as they maintain the medially placed actomyosin ring Moreover both Cdc7p and Sid1p remain associated...
- 198
- 156
- 0
THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II
... 2008) Determining protein-protein interactions in large protein complexes is key in understanding the function of each constituent, as well as determining the regulatory mechanisms for the complex ... begin to process the lengthening mRNA at the 5’ end As the RNAPII moves along the transcribed region of the gene, the shift of the CTD phosphorylated at serine to serine (S2-P) occurs through the ... utilizes the affinity of calmodulin located on inserted TAP tag to bind to immobilized Calmodulin to further purify the tagged protein and any associated proteins Following incubation, the suspension...
- 109
- 206
- 0
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25