na k atpase signaling and endocytosis of the na k atpase and nhe3
Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc
... signal mediates endocytosis of ZIP1 mediating ZIP1 endocytosis and protein degradation We further demonstrate that this internalization signal of ZIP1 is sufficient for the endocytosis of the ... domains of the proteins [34] Therefore, we hypothesized that a sorting signal(s) in the cytosolic domains of ZIP1 may serve as a signal for the internalization of ZIP1 The potential sorting signal(s) ... to replace the key amino acids in these potential ZIP1 endocytic signals to analyze their roles in the endocytosis of ZIP1 Expression plasmids containing cDNAs that encoded for either the wt or...
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Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
... [12,31] Kinetic experi- ể FEBS 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase ... surface of the N-domain is due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding pocket is achieved by W411 and L414 The phosphate ... experiments to the H4-H5 loop Table Eect of FITC-labeling on the phosphatase activities of Na+ / K+ ATPase and a H4-H5 loopGST fusion protein Labeling of pig kidney Na+ /K+ ATPase as well as the puried...
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The function of TOM1 l1 in bridging EGFR signaling and endocytosis
... Src) that lies at the end of the C-terminal is essential for auto-regulation in SFKs (Brown and Cooper, 1996) For Src, the SH3 and SH2 domains are at the back of the kinase domain The SH2 domain ... 1999, Kranenburg et al., 1999, Wang et al., 2002) These data imply that the internalization of EGFR can regulate both the nature and magnitude of signaling events and the biological response of ... linker; catalytic domain and R: Regulatory domains (Adapted from Martin GS, 2001) Src is a non-receptor tyrosine kinases of SFKs Src family kinases consist of Fyn, Yes Lck, Hck, Blk, Fgr and...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... brain, heart and testis [4,5] JNK is phosphorylated and activated by the MAPK kinases MKK4 and MKK7 [6,7], with MKK7 primarily responding to cytokines, whereas MKK4 is preferentially activated ... dephosphorylate the Tyr and Thr residues in the activation loop of MAPKs and thereby attenuate signaling [17,19] In addition, there is a group of low molecular mass DSPs that lack the regulatory N-terminal ... phosphorylation of both the p46 and p55 isoforms To confirm functional activation of the JNK signaling pathway, we performed a solid-phase kinase assay using the downstream transcription factor c-JUN as the...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... (residues K4 57, K4 85R486, D50 2K5 03, K5 12D513, D59 4K5 95, E692, and K7 40) Because of an earlier study that highlighted the role of bulky hydrophobic residues in interaction of mammalian WASP and WIP ... Function of Vrp1p C-terminal module T Thanabalu et al organism for functional analysis of actin cytoskeleton components The basic elements of the yeast actin cytoskeleton are cortical actin patches and ... Function of Vrp1p C-terminal module fragments comprising residues 1–70 (KLK4 5K4 6AET – the original WH2 domain), 270–364 (residues not yet mapped), and 465–533 (KK485R486DDR) We have named the novel...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... groove of the kinase and thus acts as a selective inhibitor of insulin signaling Consistent with this observation, overexpression of Grb14 in CHO-IR cells impairs Akt and ERK insulin signaling ... PDK-1 [14], IRS-1 [13], and tankyrase [15] The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phosphorylation of Grb14 and its inhibitory effect on IR kinase and ... and the EGF receptor at high levels, we performed a comparative analysis, in time studies, of the in vivo effects of insulin and EGF on the contents of Grb7 and Grb14 in the plasma membrane and...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... amino acids of PPI1 may participate in the interaction with the H+ -ATPase and makes PPI1588His6 a suitable tool to study the mechanism of action of PPI1 The analysis of the pH dependence of PPI1induced ... XL10 and the frame and the identity of the cloned fragment verified by sequencing The construct with the N-terminal portion of PPI1 has been previously described [22] The DNA fragment coding for the ... activity of the enzyme whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s Results The C-terminus of isoform of the PM H+ -ATPase of A thaliana (AHA1) interacts with the...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... corresponding to the immunoreactivity of ERK1/2 and phospho ERK1/2 (A,B), and MEK1/2 and phospho MEK1/2 (C,D) The lower part of each panel reports the quantitative analysis of phospho ERK1/2 (A,B), and phospho ... Dhakephalkar, A., Weber, M.J., Ravichandran, K. S & Gonias, S.L (2000) Urokinase-type plasminogen activator stimulates the Ras/ Extracellular signal-regulated kinase (ERK) signaling pathway and ... that PKC activation may both result from GPCR- and receptor tyrosine kinase (RTK)-mediated signaling and that the activation of ERK by GPCRs closely parallels that employed by RTK [33] On the whole,...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... functionally important One of these, the TRP sequence, includes the start of the C-terminal helix as well as the structured loop between the 1440 ATPase domain and the C-terminal helix and in the ... contains the b domain (b strands and 8), the final helix of the AAA domain (a helix 10) and the C-terminal helix (a helix 11) This C-terminal region of Vps4 has been defined in the PFAM database as the ... Role of the Vps4 C-terminal helix P R Vajjhala et al into internal vesicles that form by invagination of the limiting membrane of the endosome The internal vesicles give the endosome the appearance...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
... hLf)4N and rhLfEGS inhibited the binding of HB-19-fluo to CHO 618 cells to an extent similar to that of the native hLf The noninvolvement of sequence 28RKVR31 of hLf was further confirmed by the lack ... glycoproteins, the gp120–gp41 complex, to attach cells and induce the fusion of virus and cell membranes The receptor complex for HIV entry consists of the CD4 molecule and at least one of the members of the ... implicated in the internalization of Lf should be different from that of midkine, although both of these ligands require the presence of accessible surface nucleolin Interestingly, both Lf and midkine...
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Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
... recently shown that the Na+ /H+ exchanger is regulated by MEK-dependent (mitogenactivated protein kinase/extracellular signal-regulated kinase kinase-dependent) specific signaling and that the cytoplasmic ... concentrations of the fractions, together with the relative amount of Na+ /H+ exchanger present in the supernatant and the pellet [19], it was found that over 80% of the Na+ /H+ exchanger remained in the ... amount at the 105–110 kDa size The amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top of the gels The amount of aggregate present at the...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... for the subunits A and B of V -ATPase The intracellular localization of these isoforms and the combination of the isoforms is yet to be determined We are now trying to express fusion proteins of ... composed of the subunits A–H with molecular masses of 14 –70 kDa, and the VO domain is a 260-kDa integral complex composed of the subunits a (100 kDa), c (17 kDa), c0 (17 kDa), c00 (23 kDa) and d ... Tacking the proteinase problem in Saccharomyces cerevisiae Methods Enzymol 194, 428 –453 Tanaka, K. , Nakafuku, M., Tamanoi, F., Kajiro, Y., Matsumoto, K & Toh-e, A (1990) IRA2, a second gene of...
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Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt
... decrease of the Na+ affinity (at pH 8.2–8.3) and an eightfold increase of the Na+ concentration required for half maximal inhibition Upon further increasing the pH, the Na+ affinity of the mutant ... Na+ and H+ movements across the membrane to the decarboxylation of carboxybiotin The model shows the approximate location of important residues of helix IV, helix VIII and of region IIIa of the ... in the presence of 360 mM NaCl The most dramatic effect of the R389A mutant is a shift of the pH optimum by more than 2.5 pH units to the alkaline compared to the wild-type This shift in the...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... explanation is that this inhibitor does not block the activity of another IKK isoform, IKK1 Consequently, IjBa may still be phosphorylated by the IKK1 isoform when the IKK1 isoform is present and ... most of which activate the IkappaB kinase (IKK) complex This crucial component in the NF-jB activation cascade typically consists of two catalytic subunits [24,25], IKKa (IKK1) and IKKb (IKK2) ... oscillation in the original model and in the updated model with newly measured kr1, ka1 and kd1 for IKKIjBa complex (D) In the original model kr1 (kcat) for IKKIjBa was 4.07 ã 10)3 s)1 In the updated...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... proteins: effect of cholesterol depletion by MbCD Cell Sample Donor/ epitope Acceptor/ epitope FRET efficiency E (% ± SEM) Kit22 5K6 Kit22 5K6 Kit22 5K6 Kit22 5K6 Kit22 5K6 Kit22 5K6 Kit22 5K6 Kit22 5K6 CD48 CD48 ... adjusted to 40% and the homogenate was placed at the bottom of an SW41 tube (Beckman Instruments, Nyon, Switzerland) It was overlaid with mL of 36% and mL of 5% sucrose in TKM buffer and centrifuged ... of the intracellular domains of b and cc chains followed by association with Jak, Syk (or src family) kinases These, in turn, phosphorylate the receptor chains, forming docking sites for further...
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Báo cáo khoa học: A biophysical view of the interplay between mechanical forces and signaling pathways during transendothelial cell migration doc
... protein kinase cascade, and proteolysis leading to apoptosis Activation of the NF-jB pathway leads to recruitment and activation of IjB kinase kinase; the phosphorylation and activation of IjB kinase ... flow and effects from other neighboring cells and matrix The biophysical aspects of the endothelium through which the leukocytes transmigrate, in addition to the biophysical aspects of the leukocytes ... kinase by IjB kinase kinase; the phosphorylation of IjB; and the degradation of IjB, which releases the NF-jB NF-jB then localizes to the nucleus, where it initiates transcription of many genes...
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
... classes of signaling molecules, including Lck, Jak3, and Emt The tyrosine located at position Y691 was also contributing to the phosphorylation of the wild-type siglec tail by Lck and Jak3 kinases ... results The kinase assays indicate that the cytoplasmic domain of the siglec protein can be phosphorylated by representatives of at least three of four major families of kinases: Jak3, Lck, Emt ... Kinase assays To determine possible signaling interactions of the siglec-10 cytoplasmic domain with major signaling molecules in the cell, kinase assays were performed using representatives of...
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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx
... development and function of iNKT and NKT-like cd T cells Itk and i NKT cell development NKT cells are a subset of innate T cells characterized by their expression of the NK1.1 marker along with the ab ... in the fractions in the periphery [20] In the absence of Itk, there are reduced numbers of iNKT cells in the thymus and periphery [21] More detailed analysis of the development of Itk-null i NKT ... (2011) 1970–1979 ª 2011 The Authors Journal compilation ª 2011 FEBS 1971 Itk and i NKT and cd T cells Q Qi et al Fig Involvement of Itk in the development of i NKT and NKT-like cd T cells During...
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Báo cáo khoa học: Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase pptx
... the luminal membrane Taken together, these findings show that both NPRA and natriuretic peptides (ANP, BNP, CNP, and urodilatin) are present on the luminal side of the collecting duct, where the ... 4162–4169 Nakao K, Ogawa Y, Suga S & Imura H (1992) Molecular biology and biochemistry of the natriuretic peptide system I: natriuretic peptides J Hypertens 10, 907–912 10 Ogawa Y, Nakao K, Nakagawa ... by natriuretic peptide C and its receptor, natriuretic peptide receptorB J Biol Chem 269, 10729–10733 13 Kishimoto I, Tokudome T, Nakao K & Kangawa K (2011) The cardiovascular significance of the...
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cognitive psychology in and out of the lab. 4th ed. - k. galotti (wadsworth, 2008)
... confusing the length of the row with the numerosity of the row In other words, a typical child of this age regards the number of buttons as being the same thing as the length of the row they are ... intellectual (and other) abilities Nativism, by contrast, emphasizes the role of constitutional factors of native ability—over the role of learning in the acquisition of abilities and tendencies Nativists ... Berkeley, and Immanuel Kant These philosophers also debated the nature of mind and knowledge, with Locke, Hume, Berkeley, and Mill following Aristotle and a more empiricist position, and Descartes and...
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