mp wolters d yates jr 3rd 2001 large scale analysis of the yeast proteome by multidimensional protein identification technology nat biotechnol 19 242 247
Expression dynamics of the hepatic mitochondrial proteome of the sod2+ mouse in response to troglitazone administration
... to the adverse effects of drug and new paradigms are urgently needed to explain the unpredictable nature of idiosyncratic DILI 1.2 Troglitazone as a model drug for the study of idiosyncratic DILI ... 199 8) This highlighted the wide-ranging implications of mitochondrial diseases in many organs Many lines of evidence have also implicated inherited mitochondrial defects (mtDNA and nDNA encoded) ... significantly alter the labelled proteins’ Mr, and even if they do, the labelled proteins should move in tandem Therefore, the same protein found in both sample pools but labelled with different Cydyes will...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
... resulted in abrogation of the fast phase of the reaction, as compared with inactivation in the absence of DEGRFXa Addition of 25 IUÆmL)1 UFH in the presence of DEGR-FXa stimulated the APC-mediated ... started by the addition of 3.9 nM wildtype APC Samples were withdrawn from the inactivation mixture and subjected to SDS/PAGE Subsequently, proteins were blotted on a poly(vinylidene difluoride) ... published experimental data To develop our two final models of the FVa–APC complex, we merged the two sets of computations reported above and performed limited interactive reorientations of the two proteins...
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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... on [GdmHCl] DGunf is the Gibbs’ free energy of unfolding in the absence of denaturant and m represents the dependence of the unfolding free energy on [GdmHCl] For the fitting of the peroxidase ... mechanism of release of the axial ligand under alkaline conditions and in the presence of GdmHCl is different Under alkaline conditions release of the axial ligand is postulated to arise from the deprotonation ... networks of protein were constantly observed in the drops and these were repeatedly dissolved by addition of lL of water Attempts to slow down the crystallization process with 8.7% glycerol in the...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... performed at pH 7.0 and pH 8.5, as described previously [25] The protein preparation was concentrated and freed of lipids as follows The sample was dialysed extensively against distilled water and ... resuspended in 10 mM K-phosphate/0.05 mM EDTA (pH 7.4) Protein concentration was determined by the method of Bradford [33], using BSA as a standard Microsomal proteins separated by SDS/PAGE were ... °C under N2 atmosphere Incubation was carried out under N2 for 30 at 37 °C with 0.24 mg of microsomal proteins and terminated by the addition of mL of 20% KOH in 50% methanol, followed by additional...
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Báo cáo khoa học: Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe pdf
... standard addition method, and the RSD was obtained from a series of six cell extracts (Table 1) The results indicated that the recovery and precision of the method applied to determine O2–Æ in the ... concentration To assess the selectivity of the method, the effect of other ROS and biological compounds on the determination of 3.33 lm O2–Æ was examined individually An error of ± 5.0% in the relative ... phenyl ring) In order to validate the molar ratio of the reaction between the probe and O2–Æ, we synthesized pure DBZTC oxide and tested the fluorescence intensity of the oxide at different concentrations...
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Báo cáo khoa học: Identification of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx
... located in the core of the CCP structure (Fig 3A) Furthermore, all of the additional insertions of the extended MCA2590 sequence are introduced in loop regions on the surface of the structure, thereby ... followed by an AT-dominant stretch of nucleotides was found downstream of the mopE gene [9] The lack of significant predictions of promoter and terminator regions immediate 5¢ of mopE indicates ... [8] The resulting fractions were assessed by SDS ⁄ PAGE, demonstrating the presence of the large subunit of the methanol dehydrogenase (MeDH) in the soluble fraction and the outer membrane proteins...
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Báo cáo khoa học: "PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases" doc
... lycedaxeh %01( lCaN/BATC dna lCaN M fo noitidda eht yb detanimile erew sedirahccasylop dna ,sirbed llaw llec ,nietorp derutaned ehT nim 03 rof Co56 ta detabucni saw dna ,)lm/gm 01( K esanietorp dna ... gnidocne seneg eht dedulcni sremirp editoelcunogilo eht gnisu detagitsevni stnanimreted ecneluriv ehT reffub ET fo lµ 05 ni dednepsus dna ,lonahte %07 ni dehsaw ,snoitulos )M 3( etateca muidos dna ... eneg dna ,ylevitcepser ,smraf dna ,4 ,7 morf setalosi dna ,01 ,02 rof pb 019 dna ,017 ,726 fo stcudorp tnereffid eerht dedleiy eneg aoc eht fo noitacifilpmA )nwohs ton atad( succocorciM dna ,iccocolyhpats...
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Tài liệu Int’l High Performance Network Infrastructure of Korea doc
... Testbed KR, SG & JP, IPv6 Based High Performance Multimedia Conferencing over the APII Testbed KR & US, Development of Distributed Technique and Methodology of International Collaboration for the Analysis ... TEL14 in 199 6 APII Cooperation Center launched in Seoul, Korea in 199 6 Networks Created (199 9 -2001) Korea & Japan, Korea & Singapore (199 9) Korea & US (2001) Joined TEIN (2001) Expanded via ... Oct 2000) - Co-proposed by KR, SG and EC TEIN EGM-1 (Seoul, Mar 2001) - Discussed how to launch TEIN 1st Generation of TEIN launched (5 Dec 2001) - Between FR (RENATER) and KR (KOREN) EGM-2...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
... following the second mitosis Sizes of plasmodia were then of the order of 4–7 cm2 The third metaphases were observed at 25.6 ± 0.5 h (mean ± SD, 10 replicates) after the fusion of microplasmodia for the ... and no attempts were made to standardize the ELISA readings on an H1 mass basis Series of measurements were compared after standardization with a reference sample prepared by the same method ... 7.5, mM dithiothreitol, mM MnCl2, and 100 lgÆmL)1 BSA Polymalate was quantitated as described by Karl et al (2003) [10] DNA was measured by the method described by Gold and Shochat (198 0) [22]...
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Child Health And The Quality Of Medical Care by Sarah L. Barber University of California, Berkeley ppt
... and provider, and whether provider performance accorded with established standards of care The specific case scenarios constructed measure the process quality of prenatal and child healthcare These ... insults at critical periods of development during the prenatal period and the first two years of life (Martorell, 199 9; Morris et al 199 8; Gould, 198 9) One-third of children younger than five years ... substituting the determinants of the chosen health behaviors into equation (1) for the xht To derive the determinants of the xht, we make the standard assumption that households make decisions by maximizing...
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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx
... antibacterial protein detected by amino acid sequencing analysis are indicated by boxes The predicted dinucleotide-binding motif and the GG-motif are indicated by a straight line and a broken line, ... assayed using the growth-inhibition plate method The pH of each fraction is indicated by a diamond Pooled fractions indicated by bar (III) were used for M urea ⁄ PAGE (D) SDS ⁄ PAGE of the antibacterial ... 102–104, and the open reading frame was composed of a 1566-bp region, encoding a protein of 522 amino acid residues A BLAST search demonstrated that the encoded antibacterial protein shared identity...
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The Project Gutenberg EBook of Creating Capital, by Frederick L. Lipman pot
... upon the surrounding evidences of civilization—buildings and railroads and highly cultivated fields, the machinery of production and distribution, the shops full of useful commodities—and then ... every particle of which is due to the earnings and savings of somebody, to the surplus not consumed by the workers of the past, their unexpended and unwasted net balances year by year Universities, ... the purposes of living But during the last few years a new factor has entered into the money problems of the individual, often adding to his trials, often adding to his self-made excuses, and...
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The Project Gutenberg E Book of Domestic Animals, by Richard L. Allen ppt
... the United States, their progress during the various stages of their improvement, and the comparative value of the improved and ordinary breeds A knowledge of the best mode of breeding and management ... Entered according to an Act of Congress in the year 1847 By RICHARD L ALLEN, In the Clerk's Office of the District Court of the United States for the Southern District of New York INTRODUCTION The ... longevity of the breed, and the stamina of the individual 11th No violent cross, or mixing of distinct breeds, should ever be admitted for the purposes of perpetuation, as of cattle of diverse...
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Báo cáo lâm nghiệp: "Soil environment and nutrient status of Norway spruce (Picea abies [L.] Karst.) underplantings in conditions of the 8th FAZ in the Hrubý Jeseník Mts" potx
... content of N and P and the degree of damage to underplantings caused by nutrient deficiency The degree of damage to evaluated underplantings decreases evenly with a higher proportion of these elements ... based on the frequency of occurrence of visual symptoms of deficient nutrition (chlorosis), and the general vitality of the stand concerned was also evaluated A scale of underplanting damage was developed ... spodic horizons Bs was taken by the classical sampling method directly from soil pits In this way, 19 composite samples of H horizon, 19 composite samples of Ae/Ep horizon and 19 samples of...
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Báo cáo lâm nghiệp: "Influence of exogenous L-proline on embryogenic cultures of larch (Larix leptoeuropaea Dengler), sitka spruce (Picea sitchensis (Bong.) Carr.) and oak (Quercus robur L.) subjected to cold and salt stress" pdf
... measurements, the specific growth rate of the cultures was determined We used potassium leakage from the cells to study the effect of low and freezing temperatures [14] Proline was added to day old embryogenic ... maintained for 14 days Every days, the flasks were briefly removed from the orbital shaker, the cells allowed to settle for 10 and the volume of settled cells (SCV) recorded [7] From these measurements, ... inversely proportional to temperature Addition of proline reduced the amount of potassium released at every temperature, and the protective effect was related to the concentration of exogenous proline...
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Báo cáo lâm nghiệp: " Influence of repeated defoliations by insects on wood increment in common oak (Quercus robur L)" pps
... autumn 198 0 and 199 0 from the northern and southern sides of the stems at 1.3 m The width of the 30 last annual rings was measured (± 0.05 mm), and latewood (LW) distinguished from earlywood (EW) ... were observed in the upland and solonetz stands, and the lowest in the floodplain and riverbank stands Since the crowns are more frequently damaged by insects in floodplain stands, these latter ... (Utkina and Rubtsov, 199 4) Dominant and suppressed trees responded differently to defoliation in different stand types In the floodplain stands, the reduction in increment was higher on dominant...
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Báo cáo khoa học: "A comparison of photosynthetic responses to water stress in seedlings from 3 oak species: Quercus petraea (Matt) Liebl, Q rubra L and Q cerris L" doc
... assimilation and under intense irradiance As the observed decreases resulted from both increased F and decreased F it can be , m concluded that they were the expression of some kind of damage to the PS ... transiently reduced by increased thermal energy dissipation when the rate of electron transport exceeds the need of reducing power for CO fixation; this was observed at midday under natural conditions ... this damage (Kyle, 198 7) However, loss of PS II activity should be observed only if the rate of damage exceeded the rate of repair (Baker, 199 1) Q cerris displayed the least droughtinduced sensitivity...
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