molecular biochemical pharmacological and functional analyses of zebrafish coxs

... functional complementation and gene expression analyses and drafted the manuscript XL and JX provided biochemical and molecular analysis supports SW contributed to data interpretation and to writing the ... expression of COPTs (Figure 8a) Mn deficiency induced COPT1 in root and COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly ... to exponential phase and spotted onto SC-His-Ura and ethanol/glycerol (YPEG) plates Figure S2: Analyses of the functions of rice COPTs in Fe-uptake and Zn-uptake mutants of Saccharomyces cerevisiae...

Ngày tải lên: 11/08/2014, 11:22

... Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and ... dose-dependence of the regulation of the activity of the zebrafish ST by these divalent metal cations and their modes of action Fig Effects of divalent metal cations on the sulfating activity of the zebrafish ... however, is the identification of the various cytosolic STs and their biochemical characterization We report in this communication the molecular cloning and expression of two distinct zebrafish cytosolic...

Ngày tải lên: 08/03/2014, 02:20

... members, Professor Ng Huck Hui, Dr José   R   Dinneny, and Professor Toshie Kai for their valuable comments and constructive advice My sincere thanks to all the past and current members of the Cohen ... (representative reviews in (Iorio and Croce, 2012; Mendell and Olson, 2012; Rottiers and Naar, 2012)) My PhD project is focused on identification and functional characterizations of miRNA-target interactions ... the translation of lin-14 and lin-28 mRNAs, both of which encode two heterochronic regulators in C elegans Analysis of sucrose gradients and metrizimide-buoyant density gradients of these mRNAs...

Ngày tải lên: 09/09/2015, 10:17

... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea ... Fc| RhFo, where Fo and Fc are the observed and calculated structure factor amplitudes of reection h, respectively Rfree is equal to Rcryst for a randomly selected 5.0% subset of reections not used ... structural interactions of a nucleic acid with a protein Conclusions The functional and structural studies presented herein demonstrate the attributes of N-acylsulfonamidyl and sulfonimidyl groups...

Ngày tải lên: 14/02/2014, 22:20

... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... concentration of Rep245 prepared in 10 mm sodium phosphate (pH 7.4) was lm CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal ... side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-primase is part of a motif which...

Ngày tải lên: 18/02/2014, 18:20

... describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular characterization Materials and methods Materials ... presence of several concentrations of ATP (A) or AMP (B) and different concentrations of the inhibitor Each point is the mean of three separate trials Inhibition of AK by the specific inhibitor Ap5A and ... detected after induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein The purification and functional folding of L donovani AK from...

Ngày tải lên: 21/02/2014, 00:20

... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL [21,34] support ... domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain is increasingly...

Ngày tải lên: 21/02/2014, 15:20

... for detection of an additional CRFR1e1 protein (band 10) with molecular mass of 20 kDa (Fig 2) Proteins with lower molecular mass than expected included band (39 kDa) for CRFR1f, band (34 kDa) ... fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP accumulated ... boxes, insertion of a cryptic exon (B) The structure of constructs used for functional assays (C) The structure of constructs used for Western blotting In general the majority of our isoforms were...

Ngày tải lên: 07/03/2014, 15:20

... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... Expression and biochemical characterization of VBARP The predicted sizes of the proteins encoded by VBARP-L and VBARP-S are 69 and 49 kDa, respectively To test this, C-terminal V5-tagged VBARP-L and ... expressed the predicted molecular mass of protein similar to the in vitro 4095 Molecular characterization of ANKHD1 splice variant A B Fig Expression of VBARP isoforms in vitro and in vivo: (A) In...

Ngày tải lên: 07/03/2014, 21:20

... and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS analyses ... encoded 170 amino acid polypeptide has a predicted molecular mass of 19 216 Da and a pI of 4.69, in good agreement with MALDI-TOF MS (Fig 1A) and chromatofocusing data, respectively The other three ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary...

Ngày tải lên: 16/03/2014, 11:20

... phylogenetic analyses of the elapid PLAs and the krait 3FTx were carried out to Venom proteins of Bungarus fasciatus better understand the intrageneric and intergeneric variations of kraits and their ... mutational rates of the exon of the 3FTx genes are much faster than those of the PLA genes, leading to high variation of KBf-3FTx Cloning and cDNA sequencing Venom glands of only one of the KBf specimens ... present in B candidus venom, while 3FTx-RK is 84% identical to bucain [25] of B candidus Calcium binding and kinetic parameter of the P31-PLAs Four PLAs (Va, Vb-2, VI and II) of KBf and Bf contain...

Ngày tải lên: 16/03/2014, 12:20

... denaturation at 94 °C for 30 s; five cycles of 94 °C for s and 72 °C for min; five cycles of 94 °C for s and 70 °C for min; and 20 cycles of 94 °C for s and 68 °C for The RACE–PCR product was then ... rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression levels of the ... Custommade software and hardware were used for acquisition and analysis of data Leakage and linear capacity currents were subtracted by using P/4 protocols Data were sampled once every ls and were...

Ngày tải lên: 16/03/2014, 23:20

... and LHCII monomers) (band 2), and tri4620 meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores Bands and contained supramolecular complexes of PSII with Lhcb proteins (Fig ... 484.5 Band 864.9 133.0 Band 100.0 100.0 Band 412.2 14.3 Band 6+7 20.9 89.9 Total Band Monomeric Lhc 50.5 Band Band Free pigments 37.4 2046.7 872.2 Band LHCII trimer C B6 B7 WT Zb63 WT Zb63 Band ... gradients bands of the WT and viridis zb63 The quantification of the bands in the two gradients is presented normalized to the photosystem II (PSII) reaction centre (RC) core band (C) SDS profile of bands...

Ngày tải lên: 30/03/2014, 10:20

... Transfection of TRPV1 in dorsal TRP channels and cytoskeleton regulate each other root ganglia-derived F11 cells results in co-localization of TRPV1 and microtubules and accumulation of endogenous ... stabilization of the microtubule results in the induction of neuritogenesis and the formation of elongated cells (C) Time series of a growth cone developed from F11 cell expressing TRPV1–GFP Application of ... cytoskeleton TRPV4 is a member of the TRPV subfamily and a close homologue of TRPV1 It is activated by endogenous endovanilloids, by temperatures of > 37 °C, and by both hypo- and hyperosmotic 4690 stimuli...

Ngày tải lên: 30/03/2014, 10:20

... mammals and insects Further modifications of residues that belong to the aromatic cluster and positive charges may be useful for final determination of the toxic site and for clarification of the molecular ... BmK (BmKIM, BmKAEP and BmKIT2), but Arg6 or Lys6 and Thr57 are conserved in other scorpion species Cloning and analysis of genomic sequence of BmKIM We have isolated, cloned and sequenced the ... potential of )80 mv on ventricular myocyte and DRG neuron A decrease in the peak sodium currents is caused by BmKIM (B) Relationship of voltage and sodium currents in the presence and absence of BmKIM...

Ngày tải lên: 31/03/2014, 09:20

Ngày tải lên: 14/11/2014, 08:21

... the formation of the craniofacial skeleton The functional diversity of Wnt signaling is reflected by a huge set of different ligands (15 Wnts in zebrafish) and receptors (11 Fzs in zebrafish) However, ... (HN) B-D and I-K are higher magnification views of cleithrum boxed in A and H, respectively Operculum of a DMSO control (E-G) and Mtz treated larva (L-N) Note: Loss of CFP and absence of mineralization ... on issues of skeletal development and disease This is because both medaka and zebrafish provide numerous advantages for this type of research: They frequently produce high numbers of offspring...

Ngày tải lên: 10/09/2015, 08:43

... pursuit of a graduate degree possible i Tables of Contents Page Acknowledgements i Table of Contents ii Summary viii List of Tables xi List of Figures xii List of Abbreviations xvi List of Publications ... 27 1.18 Notch and diseases 29 1.19 Notch and p53 30 1.20 Regulation of Notch signaling by p53 32 1.21 Zebrafish: The model system 33 1.22 Advantages of using zebrafish 34 1.23 Zebrafish mutagenic ... Formation of morphologically distinct somites 36 1.25 Somite patterning 37 iii 1.26 Aims of this study 39 Materials and Methods 44 2.1 Zebrafish maintenance and embryos 44 2.2 Irradiation of zebrafish...

Ngày tải lên: 14/09/2015, 14:03

... GENETIC STUDY OF HEMATOPOIESIS IN ZEBRAFISH — CHARACTERIZATION OF ZEBRAFISH UDU MUTANT, POSITIONAL CLONING AND FUNCTIONAL STUDY OF UDU GENE LIU YANMEI (Master of Medicine, Peking University, ... forefront of basic science, helping us to understand cell biology, molecular biology, genetics, and protein structure and formation On the other hand, from the clinical point of view, a basic understanding ... China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR AND CELL BIOLOGY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE Acknowledgements I would...

Ngày tải lên: 14/09/2015, 17:54

... BIOPHYSICAL PROPERTIES OF E6 AND E7 33 3.1 Biophysical properties of the E6 and E7 proteins: Previous data and concept of this study 33 3.2 Biophysical properties of the E7 protein: Experimental ... sequence of HPV16 E6 and predicted secondary structure Figure 27: Model of combinatorial binding modes of domains of E6 Table : Stoichiometric ratios of zinc to S-E6 after individual steps of purification ... casein kinase II and the location of the zinc binding domain are indicated The HPV E6 protein consists of two tandem copies of this domain and it has been speculated that E6 and E7 may have evolved...

Ngày tải lên: 17/09/2015, 17:18