... containing Fronting and Khởi ngữ were categorized into functionaland structural groups In the functional group, samples containing fronting were categorized into four minor groups: Linking with ... features of Fronting in English and “Khởi ngữ” in Vietnamese? (2) What are the similarities and differences in the structure and function of Fronting in English and “Khởi ngữ” in Vietnamese? Scope of ... functions and structures of fronting in English and khởi ngữ in Vietnamese 2.3 Data collection 50 sentences in English containing the fronting structure and 50 sentences in Vietnamese containing khởi...
... structure consisting of repeating galactose and N-acetylglucosamine ! "*! Introduction units Studies have shown its importance in maintaining the structure and arrangement of collagen fibrils in the corneal ... 2001) This is in support of previous studies illustrating the requirement of heparan sulphate chains for sequestering and binding of FGF-2 to its receptor within the ECM (Bernfield and Hooper, ... Histopathology of the Prostate Gland 1.1.4.1 Normal histology of the prostate Columnar secretory cells line the ducts and acini of the prostate gland These ducts and acini are regularly spaced and are...
... contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands andin the a-crystallin domain of monomers C and B, respectively (Fig 8) A more prominent dimer–dimer interface ... against physiological stress, was investigated by site-directed mutagenesis of a-crystallin domain residues and molecular modeling of protein structure The p26 a-crystallin domain contains nine ... C-terminal extensions which extend to neighboring monomers, and the remaining b-strands, with the exception of strand 6, are arranged in two antiparallel beta sheets within the a-crystallin domain...
... claudins ‘Non-classic’ claudins Claudin-10b Claudin-15 Claudin-9 Claudin-11 Claudin-6 Claudin-4 Claudin-18a Claudin-3 Claudin-18b Claudin-20 Claudin-12 Claudin-14 Claudin-2 Claudin-19b Claudin-19a ... Claudin-16 Claudin-7 Claudin-1 Claudin-21 Claudin-24 Claudin-22 Claudin-23 Figure A phylogenetic tree of full-length human claudin proteins, indicating the relationships between them Claudin-10, ... are just emerging and will probably become a major focus of research in the field of claudins and TJs From a practical point of view, a better under tanding of TJ s formation and regulation...
... carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) A younger member of the household ... (IADL)" and included ability to travel outside, go shopping, prepare own meals and handle money A limited number of tests of physical performance for assessing the functioning of upper and lower ... the time of the interview was identified as an "informant" At each interview where an informant was present, assessment of the informant of the health status of the elderly person was obtained Results...
... E185, D226, D244) in PIN domains involved in binding Mg2+ ions are conserved in all three sequences Other conserved residues are R263, V139 and N144 Determination of the MCPIP domain architecture ... proteins interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the ... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and...
... Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of both TnC and Ca2+ Ac, actin; Tm, tropomyosin; ... TnCbinding site and the C-domain of TnC accompanying Ca2+ binding to site IV of TnC In vertebrate troponin, the activation may be a result of the interaction between the inhibitory region and ... 116– 131) In the relaxed state, the inhibitory region binds to actin and inhibits actin–myosin interaction [11,12], while in the contractile state, Ca2+-binding to site I and ⁄ or II of TnC causes...
... arose resin a second chromatography step using the same resin was required (gradient of 15–150 mm NaCl contained in L buffer A) to obtain pure protein Redox potentiometry, ligand binding and stopped-flow ... ligand and substrate binding For those members that contain a His-His pair, there has been no report of a systematic analysis of the contribution of each histidine residue to binding and catalysis ... be involved in ligand binding and counterpart residues in related enzymes and also the location of the tyrosine residue implicated as proton donor in OYE B constant for enzyme-ligand complexes,...
... H pylori strains harbouring a functional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro -in ammatory production of cytokines, mainly interleukin (IL)-8 [12] ... the first and last turns of helices E and F, and the C-terminus helices I and J In addition, there is a lysine-rich N- and C-terminus, in accordance with the basic isoelectric point of CagS However, ... anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers also involves portion of chains D and E of the two monomers,...
... fatty acylation and, thereby, be involved in membrane anchoring of the pilin Since PilA4 is similar to the meningococcal pilin and contains several central serine residues, it is tempting to speculate ... secretin-like proteins of type IV pili and type II protein translocation machineries are known to form ring-like structures in outer membranes The presence of PilQ in T thermophilus inner and outer ... Analyses of novel Thermus competence proteins J Rumszauer et al N-terminal domain of PilQ, and the colocalization of PilQ with PilW in inner and outer membranes and the results from the pilW and pilQ...
... protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of input proteins ... variant A Fig Interaction of Vpr and VBARP proteins in vitro andin vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK...
... (below 1%) were found of the monoterpenoids a-thujene, sabinene, a-terpinene (E )-b-ocimene, terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library ... proteolytically processed into their mature forms Plastid targeting signals are typically rich in serines and threonines and low in acidic and basic amino acids and about 45–70 amino acids long Usually ... %Sj) of (–)-a-pinene The sabinene side product of Cl(–)bPINS was determined to be 74% e.e of (–)-sabinene (Table 2) ClcTS produced (–)-a-pinene as a side product with an e.e of 24%, but (+)-b-pinene...
... Table 2-1 and 2-2 2.7.5 Vitamins andminerals For selected minerals (iron, calcium, selenium, and iodine) and a limited number of vitamins (folate; vitamins E, B6, and A), the clinical and epidemiological ... significant role in maintenance of optimal health and are limiting in diets worldwide In addition, there is a growing knowledge base indicating that elevated intake of specific vitamins andminerals (for ... metabolic engineering in plants so challenging In conjunction with such increases in the understanding of plant metabolism, the challenge then remains to understand how components in the diet interact...
... protein–protein and DNA–protein interactions [36] The name of this domain derives from its original identification in the proteins SWI3, Rsc8 and Moira, which are ATP-dependent chromatin remodeling ... in the eukaryotic cell nucleus, and because it is one of the first examples of the insertion of a protein–protein interaction module (the Tower domain) into a highly conserved functional domain ... is in agreement with the domain organization in the first line The third line shows a summary of the identified linear motifs (after filtering processing taking in account the accessibility of the...
... structures of CMP and UMP, and interactions between the cytosine moiety of nucleotide and various side chains of wild-type CMP kinase (Upper) Chemical differences between CMP and UMP are indicated in ... Calculating proton uptake release and binding free energy taking into account ionization and conformation changes induced by proteininhibitor association: application to plasmepsin, cathepsin D and ... with enzyme binding and substrate catalysis Kinetic and nucleotide-binding properties of E coli CMP kinase variants with ATP as variable substrate Determination of the reaction rates of different...
... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P-450scc Effect of cholesterol, ... was in the range of values determined in previous studies for the interaction between bovine Adx and bCYP11B1 Biacore measurements were performed to investigate the binding behavior between bovine ... formation of hydrogen peroxide, other NADPH consuming reactions probably involving the redox partners AdR and Adx are taking place Further studies will focus on investigating this interesting question...
... in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin [30–35] As Ig-C2 domains may serve as molecular spacers and bind to a diversity of ligands, ... domain in myosin light chain kinase A Fig Prediction for a conserved aggregation domain in the 4Ig region of L-MLCK and recombinant expression of MLCK variants (A) The sequences of the N-terminal ... barrier in intestinal disease Lab Invest 84, 282–291 12 Tran QK, Watanabe H, Zhang XX, Takahashi R & Ohno R (1999) Involvement of myosin light-chain kinase in chloride-sensitive Ca2+ in ux in porcine...
... results again highlight the stimulatory role of P2Y12 in mitogen-activated protein kinase and Akt kinase signaling pathways On the other hand, the functional activity of the P2Y1 receptor in long-term ... to fragmented adhesion region in the cell center Vinculin staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) ... chemiluminescence Equal loading of proteins was ensured by b-actin concentration Control: cells were growing in MEM containing 10% fetal bovine serum 24, 48, 72, 96 h: cells were cultivated in MEM...
... library using a combination of PCR amplification, mass sequencing and library screening as described in the Materials and methods The first clone was 1689 bp and contained an ORF of 1434 bp coding for ... selection on minimal medium agar plates without uracil or leucine, cells harbouring the yeast plasmid were cultivated in minimal medium lacking uracil or leucine but containing 2% (w/v) raffinose and 1% ... manufacturer’s instructions and submitted to random sequencing by the chain termination method using the ABI PRISM Big Dye Termination Cycle Sequencing Ready Reaction Kit (Perkin-Elmer, Weiterstadt,...