methanol and methanoic acid reaction
Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt
... oil and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid ... as well as shikimic acid obtained by methods N0 1, N0 2 and N0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise ... shikimic acid that it more simple and economic than Payne and Edmonds method. In this study, we used methanol : water (8:2) extraction to remove impurities from the crude shikimic acid, while...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... before as important step in the biosynthesis of thelantibiotics epidermin and mersacidin catalyzed by theLanD enzymes EpiD and MrsD, respectively [8–10]. Flavin-dependent oxidative decarboxylation ... N210K and copurication of the 4Â-phosphopantothenoyl-CMP intermediate. His-CoaB proteins(His-CoaB and His-CoaB N210K) were puri-fied from E. coli cell extracts by Ni-nitrilotri-acetic acid and ... Escherichia coli and most eubacteria, the synthesis of4Â-phosphopantetheine, which is also the key reaction incoenzyme A biosynthesis, is catalyzed from 4Â-phospho-pantothenate and cysteine by...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents ... plates containing 50 lgặ mL)1ampicillin and 30 lgặmL)1chloramphenicol, and incubatedat 30 °C and 42 °C.Plasmid constructions, overproductions and purificationsPlasmids for overexpression ... RNaseH domain and an APase domain. The RNase H and APase activities of the full length SCO2299 proteindepend on its N-terminal RNase H domain and C-ter-minal APase domain, respectively, and do not...
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Tài liệu Báo cáo khoa học: The swinging movement of the distal histidine residue and the autoxidation reaction for midge larval hemoglobins docx
... ligand. The correspondingspectral changes in the Soret band were therefore followedby stopped-flow and rapid-scan techniques, and theobserved rst-order rate constants of koutẳ 25 s)1 and kinẳ ... resistance to acidic autoxidation, we concludethat T. akamusi hemoglobin VII, the major component, canplay an important role in O2transport and storage againstthe extremely acidic and hypoxic ... door for ligandentry. J. Mol. Biol. 207, 459–463.23. Tian, W.D., Sage, J.T. & Champion, P.M. (1993) Investigations ofligand association and dissociation rates in the ÔopenÕ and ÔclosedÕstates...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... amino acid sequence of GALA revealed veryhigh similarity to the endogalactanases of A. tubingensis(95.7% amino acid sequence identity) and A. aculeatus(78.9% amino acid sequence identity) and ... minD-galactose,D-galactobiose and D-galactotetraose were used as standards to identify theD-galactose and D-galacto-oligosaccharides. The calculatedareas forD-galactose and D-galacto-oligosaccharides ... endogalactanases (between 21% and 28%amino acid sequence identity). This is illustrated by aCLUSTALW analysis [29] of the deduced amino acid sequences of GALA and b-1,4-endogalactanases ofAspergillus...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... unchargedpolar amino acids (i.e. Q, N, S, and T) and an increase incharged amino -acid residues (i.e. K, E, and R) inhyperthermophilic proteins. As S and T can catalyze thedeamination and backbone ... The amounts of fructose and xylulose produced weredetermined using the cysteine/carbazole/sulfuric acid method. Absorbance was measured at 537 nm and 560 nmfor xylulose and fructose, respectively. ... 0.981, is based on theelution data of the 200, 150, and 66 kDa protein standards. Linearregressions (1) and (2) give molecular masses of 200 and 177.5 kDa,respectively, for BLXI.6294 C. Vieille...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... complex16.Amplification reaction was typically performed ina 50àl reaction mix containing 0.25nmoles offorward and reverse primers, 2mM finalconcentration of dNTP, 1.5 U of Taq polymerasein 1X buffer and proteinase ... These cases were as follows, two eachof asthma, pneumonia and cancer lung, one each ofpost CABG, COPD and lung abscess and 12 withcough and cold. All samples from these individualshad tested ... routinediagnostics like microscopy and cultureTable 3 presents the comparative efficiencyof PCR to diagnostics like microscopy and culture.The sensitivity, specificity, positive and negativepredictive...
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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot
... Differential effects of ligand-activated RARa and RXRa onthe promoter activity of the hGnRH II gene in TE671 and JEG-3cells. In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... TE671medulloblastoma and JEG-3 placenta choriocarcinoma cells. Supershift and chromatin immunoprecipitation assays showed in vitro and in vivobinding of NF-jB subunit p65 and the retinoic acid receptors, RARa and RXRa, ... PCR.Treatment of cells with the RARa ⁄ RXRa ligands, all-trans retinoic acid and 9-cis-retinoic acid, reduced and increased GnRH II gene expression inTE671 and JEG-3 cells, respectively. Taken together,...
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Women and Adverse Drug Reactions Reporting in the Canadian Context pot
... “client” or partner in meeting the HPB mandate; and ã a lack of new criteria for “safety”, new methods for post marketing evaluation of drugs and devices and social and ethical evaluation of new initiatives. ... drug reactions to patients and health-care providers. The jury’s first recommendation called on Health Canada to set up a joint body to review, improve and standardise all communication tools and ... Abraham and Paul Taylor, “Drug reactions kill thousands: researchers”, The Globe and Mail, April 15, 1998. 46 Namrata Bains, MSc; Duncan Hunter, PhD, Adverse reporting on adverse reactions,...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos-amine, along with sialic acid which accounted for 22 and 35% of total carbohydrate content in SFC and SFD groups, respectively.Purification ... into SFC and FFC, SFD, FFD and FFD-500 groups. SFC and SFD groups received AIN-76basal diet, whereas FFC and FFD groups received AIN-76diet, wherein starch was replaced with 5% wheat bran and 2.5% ... co-distilledwith distilled water and reduced using sodium borohydride.Excess sodium borohydride was destroyed by adding 2 macetic acid and again repeatedly co-distilled with methanol and dried in a dessicator....
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... theexception of U4 (Table 3). In U1, U6 and U7, gluconic acid (o1) has high abundance, and high levels of C1-oxi-dized lactose (o2) were observed in urine samples U2,U5 and U6.DiscussionUsing a prototype ... Neu5Ac and the adjacenthexose. No relevant ring fragments were observedbetween C2 and C3, which is consistent with a 1–3linkage between Hex and HexNAc. The ring fragmentions0,2A4 and 2,4A4, ... fragmentions0,2A4 and 2,4A4, and the lack of0,3A4, are indica-tive of a 1–4 linkage between HexNAc and the adja-cent hexose. The ring fragment ions0,2A5,0,2A5-18 and 2,4A5, and the lack of0,3A5,...
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Báo cáo khoa học: Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid An immunoglobulin superfamily member from insects as a pattern-recognition receptor doc
... the O-antigen and p arts of the i nner-core and outer-core p olysaccharide, and lipid A alone could also p artially compete f or hemolinbinding to smooth LPS. Rough mutants Rd and Re,containing ... suggesting that LPS and LTAbindtothesamesitesonhemolinandthathemolinmay have a higher affinity for LTA. Hemolin was alsoobserved to bind d irectly to i mmobilized LTA (Fig. 10).LPS and LTA are s ... micro-organis ms.We showed that hemolin binds to the surface of Gram-negative and Gram-positive bacteria and yeast, and caused aggregation of these micro-organisms. Bindingof hemolin to the surface...
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zeolites and catalysis synthesis, reactions and applications - wiley
... Jongh, and Krijn P. de Jong10 Aluminum in Zeolites: Where is it and What is its Structure? 283Jeroen A. van Bokhoven and Nadiya DanilinaZeolites and Catalysis, Synthesis, Reactions and Applications. ... 57618.4 Summary and Outlook 576References 578 Zeolites and CatalysisEdited byJiˇr´ıˇCejka, Avelino Corma, and Stacey Zones Zeolites and CatalysisSynthesis, Reactions and ApplicationsEdited ... neighbors (Si-Al0Si4) and generateno acid sites, similar to the absence of acid sites in a pure-silica zeolite. Therefore,to maximize the acidity (both concentration and strength) of SAPO materials,...
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metal-organic framworks irmof-8, zif-9, mof-199 and irmof-3 as catalysts for friedel-crafts acylation, knoevenagel, azamichel and pall-knorr reactions
... the Lewis acid sites. Reaction were carried out the reaction in methanol at room temperature, using 5 mol% MOF-199 with the benzylamine:ethyl acrylate molar ratio of 1:1, 1:1.5, and 1:2, respectively. ... 1.3 Aim and objectives of the study The Friedel Craft acylation reaction Friedel–Crafts acylation reactions of aromatic compounds with acid chlorides are considered as fundamental and important ... silica materials containing imidazolium and dihydroimidazolium salts, IRMOF-3, and ZIF-8 The Aza-Michael reaction The aza-Michael reaction of amines and -unsaturated carbonyl compounds has...
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