mediated inhibition of cell growth correlates with the induction of p21waf cip1
Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot
... pattern of CIITA -mediated inhibition of Tax-2 function in these cells, representative of the species naturally infected by HTLV-2 To this end cells were co-transfected with a fixed amount of the ... likely in the CIITA-NF-YB molecular complex, is at the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization ... in A The purified immunocomplexes were immunoblotted with the anti-NFYB and the anti-Flag antibodies for the detection of the endogenous NFYB and of CIITA, respectively The expression of the proteins...
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báo cáo hóa học: " Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy" ppt
... in the formation of retinal NV in the later stages of the OIR model In this study, we investigated the temporal and spatial distribution of microglial cells during the different stages of the ... retina, the mean number of microglial cells Page of from the three fields of each counting position (central or peripheral zone, and layer) was calculated Then, the mean and standard error of these ... position above the inner retinal vascular layer (i e in the vitreous), their round cell body without any processes, their GFP expression under the control of the Cx3cr1 promoter, and their affinity...
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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx
... pattern of CIITA -mediated inhibition of Tax-2 function in these cells, representative of the species naturally infected by HTLV-2 To this end cells were co-transfected with a fixed amount of the ... likely in the CIITA-NF-YB molecular complex, is at the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization ... in A The purified immunocomplexes were immunoblotted with the anti-NFYB and the anti-Flag antibodies for the detection of the endogenous NFYB and of CIITA, respectively The expression of the proteins...
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Báo cáo y học: "Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI" docx
... infection independently of the virus strain and producer or target cell type The magnitude of enhancement is donor-dependent and correlates with the levels of SEVI The enhancing effect of SE on HIV-1 ... SE -mediated enhancement of HIV-1 infection Furthermore, they emphasize the importance of using pooled SE samples for a valid analysis of SE -mediated enhancement of HIV-1 infection because the ... Specifically, we reduced the final concentrations of SE in cell culture by pre-incubating SE with the HIV-1 virions (rather than the target cells) and adding small volumes (usually 20 μl) of these HIV/SE...
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Báo cáo Y học: ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1 pot
... coordinated interaction of the N-terminus of MDM2 with the N-terminus of p53, and an interaction of the acid domain of MDM2 with the central domain of p53 [24] Accordingly, ligands such as the NUTLIN and ... in cell culture with chaperone proteins, providing biochemical models enabling insights into the cell biology of HSP– p53 interactions [14–16] The relevance of the interaction of mutant p53 with ... the relative affinities of p53 and E2F1 for MDM2 are not known, thus the interaction of p53 with MDM2 might affect the level of active MDM2 that can regulate E2F1 Furthermore, the regulation of...
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báo cáo hóa học: " Tumor necrosis factor-mediated inhibition of interleukin-18 in the brain: a clinical and experimental study in head-injured patients and in a murine model of closed head injury." pot
... wavelength of 405 nm The experimental part of the study was set-up on two different protocols with the aim to assess the TNF-dependent regulation of IL-18 in the murine brain: (1) The first part of the ... compliance with the guidelines of the Federation of European Laboratory Animal Science Association (FELASA) and approval was granted by the Institutional Animal Care Committee of the University of Zurich ... degeneration by induction of antioxidative pathways through activation of the transcription factor NFκb, thus strengthening the notion of neuroprotective effects mediated by these cytokines [32]...
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Ant functional group succession dynamics correlates with the age of vegetation succession data analysis of worldwide studies and a case study of a secondary tropical rain forest in singapore
... relationship with age of the forest (Figure 9) The proportions of other functional groups did not change significantly with age There was no relationship between the proportions of any functional group with ... another 40 CHAPTER ANT SUCCESSION DYNAMICS IN A SECONDARY FOREST OF SINGAPORE Introduction The main island of Singapore is located off the southern tip of the Malay Peninsula and most of the ... between the proportions of functional groups of ants and the ages of forest in Singapore, using various patches of forests at different stages of succession in the CCNR as the study sites Some of the...
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Báo cáo y học: " Evidence for natural antisense transcript-mediated inhibition of microRNA function" ppsx
... power of these families of ncRNAs within the context of a larger ncRNA sensory and regulatory network [44] These mechanisms, together with the unusual but specific response of the BACE1 system to ... miR-485-5p The reversal of mir-485-5p -mediated BACE1 protein reduction by LNA-antimiR indicates the specificity of the miRNA effect and further validates the miR-485-5p binding site in the coding ... by the presence of SNPs in the 3' UTRs of mammalian genes has been clearly documented [31-35] Variants in the binding site for miR-189 in the 3' UTR region of the SLITRK1 gene are associated with...
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Báo cáo khoa hoc:" Retroperitoneoscopic radical nephrectomy with a small incision for renal cell carcinoma: Comparison with the conventional method" docx
... the top end of the 11th rib to the border of the rectus abdominis Page of Figure The surgical procedure of the A method (No.2) It is possible to remove the flank pad and to access the retroperitoneal ... bleeding On the other hand, one of the disadvantages of the A method is larger size of the incision The incision, which is about cm, is larger than the approximately cm incision in the traditional ... in the treatment of T1a renal cell carcinoma in our hospital [7] The factors in the outcomes compared between the A and B methods were the duration of procedure, volume of bleeding, volume of...
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báo cáo khoa học: "Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin" pot
... medium is refreshed, concomitantly with the reduction in cell density, shortly before the beginning of the light period of that day The majority of the cells divide in the second dark period afterward ... first, upper semicell after the second cell division (E) Cell line 18 Upper semicell resulting from the first cell division, after which the cell died (F-I) Cell line 13 Loss of growth polarity ... study the spatial and temporal patterning of cell wall biogenesis [9] Ultimately, the plant cell morphology is determined by the composition and structure of the cell wall that governs the cell...
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Báo cáo y học: "Inhibition of HIV-1 replication by P-TEFb inhibitors DRB, seliciclib and flavopiridol correlates with release of free P-TEFb from the large, inactive form of the complex" potx
... dependent on the concentration of P-TEFb [22] Treatment of cells with DRB leads to release of P-TEFb from the large form To examine the effect of DRB treatment of cells, we treated HeLa cells with increasing ... Additionally, the loss of large form within the cell in the presence of high concentrations of P-TEFb inhibitors was demonstrated by the persistence of more of the P-TEFb remaining in the nuclear ... release of P-TEFb from the large form of the complex Treatment of HeLa37 cells with seliciclib (Fig 3C) or Jurkat cells with flavopiridol (Fig 3D) led to a gradual reduction in the amount of the...
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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... according to isoform ⁄ a of PDIP46 ⁄ SKAR The dotted lines indicate the ends of the two subregions capable of interacting with ER individually localized in the same cell compartment as ER, the nucleus, ... sequence (with the stop codon) and coded for 163 amino acids present at the C-terminus of both isoforms of PDIP46 ⁄ SKAR, including the amino acids of the RNA recognition motif (RRM) also known as the ... by manual analysis of MS ⁄ MS spectra Comparison of the intracellular localizations of the human ER and PDIP46/SKAR proteins We examined the intracellular localization of the human ER and PDIP46...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... accumulation of heme in the hemintreated HepG2 cells Taken together with the siHO- 2mediated induction of HO-1 expression, these results suggest that HO-2 rather than HO-1 may play the predominant ... Northern blot analysis of HO-1, HO-2 and GAPDH mRNAs The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal The ratio of ... expressed in all eight cell lines (Fig 1A,B) We also measured the heme content of these cell lines: it was about twofold higher in YN-1 and K562 erythroleukemia cells than in the other cell types (Fig...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... in the separation of the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of ... vitro properties of these compounds It is worth noting that the extent of the effect of these analogs on the pneumococcal cultures is very dependent on the metabolic state of the cell As shown in ... separation at the end of cell division, causing the formation of long chains of cells with only small effects on cell growth and viability, even at high concentrations of the compound [24] This...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... endothelial cell line and it is now evident that the endothelial cell plays a crucial role in angiogenesis [39] and that it might affect the growth and differentiation of neighboring cells [40] The ... ECV304 cells exposed to PD98059 (Fig 5B) Interestingly, both the inhibition of MEK/ERK pathway and the exposure of ECV304 cells to NAMI-A resulted in down regulation of c-myc gene expression with ... (d) of the indicated concentration of NAMI-A Cells were also treated with 100 lM NAMI-A + 50 lM PD98059 for h The upper part of Panel A shows representative ethidium bromide stained gels of the...
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Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc
... control of ligand binding [86] This is a result of the interaction of the distal segment of the JM, particularly the E663–S671 residues, with the C-terminal lobe of the kinase domain of the apposed ... activation of the EGFR, where the C-terminal lobe of the kinase domain of one of the monomers forming the dimeric receptor interacts with the N-terminal lobe of the apposed monomer [19,47,84] Of relevance ... EGF-stimulated cells after the depletion of intracellular Ca2+ stores [36] The EGFR -mediated activation of both phospholipases Cc and A2, together with the subsequent synthesis of a series of messengers...
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Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc
... interacts with the amino group of DA and the pyridine moiety of NMNorsal in opposition to the bidentate ligation of the catalytic iron, pulling the ligands away from the iron and allowing them to ... part of the iron coordination sphere, with the piperidine rings of the TIQs and the aminoethyl moiety of DA projecting from the binding pocket (Fig 3) A potential inter2112 action between the ... their binding sites or conformational energy components The binding site of the 6,7-dihydroxylated TIQs and DA interfered with that of the essential pterin cofactor [18,25], preventing the cofactor...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... Residue Cys26 of SSA is contiguous with its putative binding interface with the T -cell receptor The common residues of SEB and SEC3 that form their respective molecular interfaces with mVb8.2 are ... 400 g for 20 The PBMCs contained in the interphase were washed with 15–20 mL of RPMI and centrifuged for 10 at 200 g; the pellet was resuspended in mL of RPMI with 10% of human serum with mM glutamine,...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx
... being synthesized on the ribosome To investigate whether this interaction is functionally relevant for the export of Tat proteins, we monitored the effect of the intracellular TF level on the efficiency ... completion of synthesis and release of SufIHATAG8 from the ribosome in a transcription/ translation system optimized for the production of full- Fig Photo-crosslinking of TF to the signal peptide of ... of the Tat signal peptide prohibits interaction with Ffh [5,34,35] Furthermore, the ÔSec-avoidanceÕ motif in the C-terminal region of the Tat signal peptide [36] might prevent rerouting via the...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
... only within the middle segment of the a-, b- and c-chains Hence, no further classification of the sponge protein to any of the three vertebrate chains can be made In the sponge sequence, besides the ... the expression of the gene coding for this receptor, Northern blot experiments were performed The results revealed that the expression level of the glucanbinding protein at the beginning of the ... of incubation with curdlan, the cells that are primarily located around the canals react with the antisense probes (Fig 7A, b; Fig 7B, b) After a longer incubation (for days) the density of the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25