maxi and mini ferritins minerals and protein nanocages
Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf
... analyses included FN tests on the 20 wheat samples, and protein and ash determinations on the flours MATERIALS AND METHODS Grain and Flour Samples and Subsequent Treatments Experiment Determination ... Falling Number, Protein, Ash, and Moisture Tests Standard methods were used for the analysis of samples for FN, protein (N × 5.7), and ash by Approved Methods 56-81B, 46-30, and 08-01, respectively ... Z., and Pomeranz, Y 1994 Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles Cereal Chem 71:315-320 Batey, I L., Curtin, B M., and...
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Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx
... expands the boundaries of signalling mediators, and it is therefore not unlikely that Sky and Mer could be involved in Table Affinities of Gas6 and protein S for Axl, Sky and Mer RTKs within and ... compilation ª 2006 FEBS S Hafizi and B Dahlback ¨ Function of protein S as ligand for Axl RTKs Surprisingly, only a handful of studies to date have reported functional effects of protein S that appear to ... between the type of protein S that is bound to apoptotic cells Specifically, both the free and C4b-binding protein- bound forms of protein S can bind to apoptotic cells via the protein S Gla region...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... specific for human Ras-GRF1 Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360 The PCR products and a DNA size marker, lane 1, were separated on agarose ... that the treatment of NIH3T3 and COS-7 cells with carbachol [9] and lysophosphatidic acid [11], activating both Gq- and Gi-coupled receptors, induces the activation and phosphorylation of Ras-GRF1 ... increase and show Ca2+ dependence of serotonininduced ERK1 ⁄ phosphorylation and a mandatory role of the Ca2+ ⁄ CaM-binding IQ domain in RasGRF1-stimulated ERK1 ⁄ phosphorylation Thus, both cAMP and...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... anti-a, -b, -c, -d, -e, and -f for immunoblotting were obtained from Gibco (Grand Island, NY, USA) Rabbit anti-PKCf for immunoprecipitation and activity determination, and the antiPKCf blocking ... 20 mM 2-mercaptoethanol) and centrifuged at 100 000 g for 30 Supernatant and membrane fraction were diluted with lysis buffer (without sucrose and EGTA) and 8–14 lg of protein from each fraction ... Determination of protein kinase C (PKC) activity in crude extracts (pmolÆmin-1Æmg-1 of protein) and PKCf immunoprecipitates (pmolÆmin-1Æmg-1 of lysate protein) Hepatocytes cultured for h and 48 h were...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... saturating concentration of ligand, P the total protein concentration, and L is referred to the ligand concentration The distribution of metal ion as [M2+]free, [ML] and of [L]free was calculated ... (MSI) and force eld CVFF and ESFF (MSI), that has all parameters needed for the octahedral Mn2+ coordination and amino acids (1AQ2) This program was also employed for energy minimization and molecular ... emission band centered at 393 nm at pH The absorption spectrum of the P-pyridoxyl adduct of PEP carboxykinase exhibits a band with a maximum at 326 nm due to pyridoxyl moiety and a band at 280...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... H2O2 and peptide and protein peroxides after photo-oxidation with 1O2 generated by rose bengal in the presence of visible light and oxygen (a) RNase A (50 mgặmL)1); (b) N-Ac-TrpOMe (2.5 mM); and ... peptide and protein peroxides investigated in the current study, with GAPDH being more sensitive than GR and LDH, and inactivation of GAPDH being metalion independent, whereas inhibition of GR and ... 1O2-generated peptide and protein peroxides, presumably by acting as sacricial targets This is in accord with the known rapid depletion of GSH and other thiols (both low-molecularmass and protein- bound)...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... and was complete between and 10 of incubation In contrast, the inactivation of p70S6K by AICAr was slower and was half-maximal only at min, whereas the inactivation of ACC by AICAr was half-maximal ... that the process of activation of ACC and p70S6K by amino acids is inhibited by okadaic acid and calyculin A, two inhibitors of type and 2A protein phosphatase, and by incubation of liver cells with ... transient, being maximal at 10 before returning to basal or even lower values between 45 and 60 of incubation (Fig 1) Like anoxia, both AICAr and oligomycin activated AMPK (Fig 2) and this activation...
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GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
... 2005; Sernova and Gelfand, 2008; Wang and Sugden, 2005; Weinreich et al., 2004) In higher eukaryotes there are preferred sequences located in AT-rich islands that serve as origins (Bell and Dutta, ... called replication termini or Ter (Bastia and Mohanty, 1996; Kaplan and Bastia, 2009) The Ter sites bind to sequence-specific DNA binding proteins called replication terminator proteins that allow ... the terminator protein was cloned and sequenced and the gene was named TUS (Terminus Utilizing Substance) (Hill et al., 1989) Tus protein was purified from cell extract of E coli and shown to bind...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... misfolded proteins [5–8] and remove protein aggregation [9–12] Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, and how osmolytes correct protein misfolding ... effects that urea has on protein function [16–19] Despite significant advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship ... presence and absence of almost all naturally occurring osmolytes We report here that protein stability and activity are not largely coupled in the presence of osmolytes However, protein stability and...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... binding and the catalysis K and P denote the free concentrations of kinase and phosphatase, respectively, and Ln and Qn are the respective dissociation constants for the kinase–target and phosphatase–target ... dephosphorylation (first panel), random phosphorylation and dephosphorylation (second panel), mixed scheme with random phosphorylation and sequential dephosphorylation (third panel), and cyclic mechanism ... link between the structural, kinetic and thermodynamic aspects of complex multisite phosphorylation on the one hand, and the specific and versatile regulation of protein activation required in signaling...
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Báo cáo khoa học: WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cf pptx
... confocal microscopy and for expert help Thanks go to S Dettwiler and C Hagedorn for excellent technical assistance E Haas (Zurich, Switzerland) and G Burkard (Berne, Switzerland) were very helpful ... threonine kinases Akt and PKCf, and was located on internal vesicles via its FYVE domain These two kinases preferentially bound to ProF after WD-FYVE protein interacts with VAMP2 and PKCf hormonal ... FYVEdomain containing protein that is also localized to intracellular vesicles [18,19], or with the vacuolar protein sorting-associating protein 4, Vps4 (Fig 1B, left, lane 7), a protein involved...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective ... interconversion between Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] ... production and stable-isotope labeling of milligram quantities of proteins FEBS Lett 442, 15–19 Madin K, Sawasaki T, Ogasawara T & Endo Y (2000) A highly efficient and robust cell-free protein synthesis...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
... structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of proteins from a mixture or from a protein complex or a complete ... cross-linking and mass spectrometry for mapping three-dimensional structures of proteins and protein complexes J Mass Spectrom 38, 1225–1237 Wong SS (1991) Chemistry of Protein Conjugation and Cross-Linking ... disulfide bonds, and artificially introduced cross-links in proteins Cross-links impose distance constraints on amino acid residues that can be used to model the 3-D structure of proteins and protein complexes...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
... selection marker protein bleomycin-binding protein (BLE), as well as other introduced proteins [9] However, only moderate numbers of transformants and relatively low levels of heterologous protein expression ... C fusiformis and analyzed CfNR levels in response to different nitrogen sources Using GFP as reporter protein, we have demonstrated that 3414 N Poulsen and N Kroger ¨ the 5¢-UTR and 3¢-UTR of ... image and two different fluorescence images The green color shows GFP fluorescence and the red color depicts chloroplast autofluorescence (bar, 10 lm) (B) Comparison of gfp mRNA expression and GFP protein...
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Lecture 3 DNA RNA and protein synthesis great
... its particular protein? As in, people protein vs tree protein? The answer is DNA! The species-particular DNA sequences produce the species-particular proteins GENES code for proteins GENES ... a sugar, and a nitrogen base DNA is in a double strand The nitrogen bases have compliment partners Adenine-Thymine Cytosine-Guanine Just a note about RNARNA is single-stranded and acts ... GENES are long strands of DNA on chromosomes What is DNA? DNA is the genetic code, Instructions for heredity, Components of genes, Director of protein synthesis AND- DNA is also A...
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Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
... PAGE, and radioactive bands revealed by autoradiography Positions and molecular mass (kDa) of protein standards are indicated on the left The arrows indicate the position of phosphorylated rStkP and ... extract (lanes and 5), cytosolic fraction (lanes and 6) and membrane fraction (3 and 7) of S pneumoniae strains Purified recombinant StkP was used as a control (lane 4) Arrows indicate bands corresponding ... wild-type and DstkP strains The Coomassie blue-stained master gel of proteins between pI 4–7 contains approximately 470 protein spots After metabolic labeling and subsequent 2-DE, at least 23 protein...
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Genetic Manipulation of DNA and Protein – Examples from Current Research Edited by David Figurski potx
... 2005; Sernova and Gelfand, 2008; Wang and Sugden, 2005; Weinreich et al., 2004) In higher eukaryotes there are preferred sequences located in AT-rich islands that serve as origins (Bell and Dutta, ... called replication termini or Ter (Bastia and Mohanty, 1996; Kaplan and Bastia, 2009) The Ter sites bind to sequence-specific DNA binding proteins called replication terminator proteins that allow ... the terminator protein was cloned and sequenced and the gene was named TUS (Terminus Utilizing Substance) (Hill et al., 1989) Tus protein was purified from cell extract of E coli and shown to bind...
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Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... removed by centrifugation, and soluble proteins were resolved on a 15% SDS ⁄ PAGE gel fragments required metals to produce soluble protein The wild-type and AMPP#3-22 proteins responded in a similar ... purple acid phosphatases and methionyl aminopeptidases [6–8] In vitro refolding Wild-type AMPP and AMPP#3-22 were overexpressed and purified Subsequently, the purified proteins were denatured with ... guanidine hydrochloride and renatured by dialysis in the presence of EDTA or metals, as described in Experimental procedures Aggregated proteins were removed by centrifugation, and the proteins in the...
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Báo cáo khoa học: and protein bilinear indices – novel bio-macromolecular descriptors for protein research: I. Predicting protein stability effects of a complete set of alanine substitutions in the Arc repressor ppt
... Eqns (31) and (32)], whereas 3134 differences between R2 and R2 ext 0;ext are inferior or equal to 0.1 [R2 R2 ị=R2 ẳ for Eqns (29) and (30) ext 0;ext ext and 0.1 for Eqns (31) and (32)], and the ... (29) and (30); 0.96 and 1.03 for Eqns (31) and (32)] In Tables 11 and 12, we depict the observed, calculated [by using Eqns (29) to (32)] and residual values of tm for cases in both training and ... Tables and give the observed and calculated tm values by using models that use nonstochastic and stochastic bilinear indices as predictors [Eqns (27) and (28), respectively] for the training and...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008