mal 01 reveals close relationship to poultry h7 viruses that infected humans in british columbia canada
Báo cáo khoa học: " Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding" ppt
... were determined, affording valuable insight into LASV glycoprotein processing and identifying glycoprotein variants that may have significant implications in the pathogenesis of LASV in humans Results ... of zinc-dependent metalloproteinases, is involved in EBOV GP shedding from virus infected cells Evidence of significant amounts of shed GP in the blood of EBOVinfected animals was obtained in ... blocking of the activity of virus-neutralizing circulating antibodies Preliminary studies in our laboratory indicate that, unlike with EBOV GP, ectodomain shedding mediated by shedases (zincdependent...
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Lecture 3 DNA RNA and protein synthesis great
... protein synthesis? First-what is “protein synthesis”? It’s building, or assembling, a protein molecule from amino acids Amino acids are smaller molecules found in the food we eat or produced in ... ribosome in the cytoplasm Transfer RNA brings amino acids to the ribosome to match the sequence codes At the ribosome, Amino acids bond together and form polypeptides, Which bond together to make ... we’ll talk about today When we look at a living thing, What we see is mostly PROTEIN So, how does an organism produce its particular protein? As in, people protein vs tree protein? The answer...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... times To analyze the RNA synthesized in one round of synthesis, we performed RdRp assays in the presence of heparin in order to prevent reinitiation In a first step we determined the heparin concentration ... radioactivity incorporated into the nucleic acids was measured after TCA precipitation and plotted against the incubation time in minutes the 3¢X 98 nt As shown in Fig 6A, in the absence of heparin this ... SL-B1 In contrast to the results obtained when changes were introduced in SL-A1, none of the modifications in SL-B1 reduced RNA synthesis (Table 1) In all cases RNA synthesis was increased Altogether,...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... expression in the stomach, including the fore gut site Recently, CHH was reported in the endocrine cells of the fore gut and hind gut of Carcinus immediately prior to and during molting, which ... expression pattern to the one observed in stomach In order to analyze the efficiency of gene silencing by direct injection of dsRNA into adult shrimps, we synthesized dsRNA corresponding to CHH mature ... silencing, a stomach transcript from a L schmitti gene that encodes to a chitinase like-protein, was cloned into the pGEM-T Easy vector (Promega); this construct generated a dsRNA of 492 bp in length...
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase pot
... the solubility of the protein expressed in E coli, the C terminal 24 amino acids of NS5B were deleted and NS5BD24 was inserted into the pET-28a vector To facilitate protein expression and purification, ... were introduced at the N terminus for initiating translation, and a polyhistidine epitope tag (GSHHHHHH) was introduced to the C terminal of NS5BD24 for affinity purification The fusion protein, ... verifying that template-sized RNA products were initiated de novo from the 3¢-terminus of the template but not by premature termination or internal initiation, as suggested in reports for tomato...
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cdna preparation and characterization
... washing/binding solution Transfer the beads into the tube containing the biotinylated firststrand cDNA After mixing, gently rotate the tube for 30 rain at room temperature Full-length cDNA remains ... This results in double-stranded cDNA with a hairpin loop at the 5'end To cleave the hairpin loop that formed during the second-strand synthesis reaction and eliminate any remaining single-stranded ... Aspiration of single neurons in culture and in fixed human brain tissue section (A) Single hippocampal neuron at week in culture (B) Recording electrode apposed in close proximity to neuron (C)...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... the following hypothesis The two coiled coil motifs are involved in intra- and intermolecular binding While C1 is involved in intermolecular binding between NP molecules and supports binding of ... Figure Mapping4of regions on NP involved in binding to VP35 Mapping of regions on NP involved in binding to VP35 (A) Schematic presentation of the constructed mutants of NP with C-terminal truncations ... motif (C2) mediates an intramolecular interaction with C1 The intramolecular interaction might be involved in regulating binding between NP and VP35 (binding is enhanced in the absence of C2)...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
... strands increases simply as a result of more minus strands remains to be determined The fact that the RNA binding domain of the protein in 3Cpro is involved in stimulating RNA synthesis suggests that ... both the proteinase and the RNA binding site [25,26] While the primary RNA binding determinant of 3CDpro lies in 3Cpro, lower affinity binding determinants are located in the 3Dpol domain [27,28] ... recently shown that a mutation (3CproR84A/I86A) in the RNA binding domain of 3CDpro abolishes that ability of the protein to stimulate virus production in the in vitro system [8] To examine the effect...
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Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... the following hypothesis The two coiled coil motifs are involved in intra- and intermolecular binding While C1 is involved in intermolecular binding between NP molecules and supports binding of ... Figure Mapping4of regions on NP involved in binding to VP35 Mapping of regions on NP involved in binding to VP35 (A) Schematic presentation of the constructed mutants of NP with C-terminal truncations ... motif (C2) mediates an intramolecular interaction with C1 The intramolecular interaction might be involved in regulating binding between NP and VP35 (binding is enhanced in the absence of C2)...
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Báo cáo hóa học: "Preparation and characterization of carbon nanofluid by a plasma arc nanoparticles synthesis system" pot
... approved the final manuscript 23 Competing interests The authors declare that they have no competing interests 24 Received: 29 October 2010 Accepted: April 2011 Published: April 2011 22 25 26 ... compared to distilled water In addition, the manufacturing machine has the potential to produce the nanofluid with a variety of materials in the future In the aspect of optimal manufacturing parameters ... induces vaporized carbon to move into the collection chamber The nanofluid collection system and cooling system pre-cools distilled water to maintain a constant 3-5°C during the collection of All...
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Báo cáo hóa học: " Intermatrix synthesis: easy technique permitting preparation of polymer-stabilized nanoparticles with desired composition and structure" ppt
... ways: (i) by depositing an ink containing INPs onto the electrode surface or (ii) by depositing the INPs-free polymeric matrix followed by the in situ IMS of INPs [4,21] In the second case, the ... Applicator (K Print Coat Instruments, Ltd Litlington, Hertfordshire, United Kingdom) The IMS was applied to SPEEK membranes by sequential loading-reduction, loading-precipitation cycles or a combination ... Cu-NPs-containing nanocomposites revealed a high instability of these INPs towards oxidation in aqueous media (Ruiz P, Muñoz M, Macanás J, Muraviev DN: submitted) Taking into account that some...
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Báo cáo khoa học: "Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture" pot
... seedling origin Rooting of microshoots was achieved in same vitro and was also successful under nonsterile conditions in rooting mixture Agar media used for in vitro rooting contained no cytokinin ... level of mineral salts Cytokinins are strong inhibitors of adventitious rooting, and high-salt media had indirect inhibitory effects GD agar media and WPM (half- or full-strength) containing a low ... transferred into WPM containing a low concentration of cytokinin (BAP 0.1 mg·l and were cultured under ) -1 continuous light to induce conversion; 1218% of embryogenic cultures produced germinating somatic...
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Báo cáo sinh học: "Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer" pps
... allied to non integrative properties into the host genome (for a review see [17]) In our experiments, RNA molecules were introduced into immature and mature DCs, allowing both to be used in trials ... resuspended in PBS containing bovine serum albumin 0.1% and then incubated for 20 at 4°C with optimal concentrations of the monoclonal antibodies Membrane markers were determined by flow cytometry, ... resuspended in AIM-V at a density of 1.3 × 107 cells/mL and allowed to adhere to culture flasks for h at 37°C in a humidified incubator Floating cells were gently removed, and AIM-V containing GM-CSF...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... selfdetermining subject.31 Having stressed the ongoing centrality of Enlightenment thought in the 1790s, I want to insist that I am not interested in rethinking our critical relationship to the ... work to reconstruct the shock that Problems now and then accompanied its initial spread in Britain Writing proliferated then as something new through, in large part, writing about writing – that ... fused into a cohesive tradition that could be studied wholly in relation to itself.34 Exposure to this body of writing was thought to have all of the improving effects that were assumed to characterize...
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Cambridge.University.Press.War.Land.on.the.Eastern.Front.Culture.National.Identity.and.German.Occupation.in.World.War.I.May.2000.pdf
... banned Coming to war land 37 printing of Lithuanian in Latin letters, seeking thus to remove Lithuanians from Polish inXuence, bringing them closer to Orthodoxy by way of Cyrillic print Instead, ... oldest living Indo- Coming to war land 31 European tongue, a linguistic coelacanth fascinating to scholars Along with other Baltic tribes, they had inhabited these shores since 3000 BC In the Grand ... crept in, through the forests and down valleys, a twilight of uncertain shapes and living forms Finally, rain would begin again Winters were harsh, as Siberian winds brought inWnities of snow to...
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IELTS Writing Preparation
... Rose (to) Increased (to) Went up (to) Climbed (to) Boomed A rise An increase Growth An upward trend A boom (a dramatic rise) Fell (to) Declined (to) Decreased (to) Dipped (to) Dropped (to) Went ... gradually to 500 by 1974 and continued to decrease but more steeply to 250 in 2000 In contrast, the rate of smoking in women in 1960 was very low at only 80 in every 1,000 By 1968 this increased to ... and increased again but more steeply to 320 in 1977 The rate of female smokers then remained stable at 320 until 1984 at which point the figures began to decline and had dropped to 200 by 2000 In...
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Preparation And Practice
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25