... indicate the positionand orientation ofthe primers. Bold fontrepresents the sequence of mature miR9*and italic fonts the sequence of maturemiR9. (C) RT-PCR analysis of pri-miR9s dur-ing the ... vector. The pro-moter sizes ofthe construct are indicatedby numbers in parentheses. )44 meanslocated 44 bp upstream ofthe rst base pairat the 5Â-end of pre-miR-9-1 dened as +1.FLuc is the ... miR9-1PN5_Fluc, were amplifiedusing the primer pairs listed in Table 1, and then sequencedto determine the orientation ofthe fragments in the repor-ter vector. These constructs were then transfected into...
... G4and the sidechain of Asn122. In the second model(Fig. 1B) the structure ofthe hexanucelotide is, on the whole, unchanged and the majority ofthe interactions thatwere seen in the first ... attributed solely to the change incharge and size ofthe sidechain of this single residue, the crystal structure of RTA N122A was solved. The RMSDfrom the Caatoms ofthe wild-type RTA crystal ... and, as such, these mutantscould not be purified. To assess the effect of substitutionsmade at each ofthe arginyl residues on catalytic activity of RTA, the ability of each ofthe purified RTA...
... in coastal zone of Mekong delta consist of 18 facies which are expressed on the map ofthe Holocene deposital environments (fig.7). However, in this paper ones important of them were only ... mainly of sand (60-80%), silty clay and mollusc, therefore its colour is brownish yellow-typical colour of oxidized environment [11,12]. The elevation of these sand ridges is about 2-7m, their ... width varies from 100 to 3000m. They distributed parallel to the shore in the form of bows with a distance from each other about 3-10km. They are evidences of paleocoast existence during delta...
... this segment ofthe business accounted for just over 73 percent of the fund market at end June 2011, with the remaining 27 percent composed of non-UCITS. Table 7. Net Assets ofthe European ... billion during the first half of 2011, slightly behind the EUR 55 billion recorded in the first half of 2010. Long-term UCITS attracted EUR 87 billion during the first half ofthe year, down ... including funds of funds, except for France, Germany and Italy for which the funds of funds data areincluded in the other fund categories; (3) net sales of non-UCITS are included in "other"...
... this segment ofthe business accounted for almost 71 percent ofthe fund market at end June 2012, with the remaining 29 percent composed of non-UCITS. Table 7. Net Assets ofthe European ... including funds of funds, except for France, Germany and Italy for which the funds of funds data areincluded in the other fund categories; (3) net sales of non-UCITS are included in "other" ... assets of balanced funds reduced by 2.1 percent. Funds of funds registered an increase in net assets of 1.7 percent during the quarter. Over the first half of 2012, total net assets of UCITS...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes. The orientation ofthe ... at position E7 as the source ofthe H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... relative to other more autoxidizable globins. Areliable indicator ofthe dynamic stability of distal hemepockets in globins is the rate of reorientation ofthe aromatic rings in the heme pocket...
... affected the level ofthe mRNAfor the other ADAM, confirming the specificity of theseASOs [19]. The ASOs reduced the level of their respectiveproteins in cell lysates but had little effect on the otherprotein ... IC50 of 80 nM[14], we considered whether the APMA-induced shedding of ACE was mediated by TACE. The effect ofthe TACE ASO, along with the ADAM10ASO, on the APMA-induced shedding of ACE wastherefore ... in the presence or absence of APMA. In the majority of cases there was little differencebetween the effect of inhibitors on the constitutive shedding of ACE and the APMA-induced shedding of...
... affair is a copy of part of Gascoigne's account, of which the following is a transcript:— [Pg 20] THE OFFICE OFTHE POSTE. In the office of William Dodington, Esquire, Auditor of Her Matie. ... forward in the street; on the east side being another building answerable thereto." These premises served as the Post Office for the long period of 118 years. The first half ofthe present ... Esq., ofthe General Post Office," and another part ofthe paper contains the following paragraph:[Pg 56]— " ;The untimely death of Mrs. Freeling is lamented far beyond the circle of her...
... wake at 6 a.m., but nap for two hours or so in the early afternoon. Thus the influence on one's sleep pattern is worthy of consideration when choosing an occupation. ...
... men maintain approximately the same number of hours of unpaid work as in childless households, but the number of hours women work in the home rises to 52 per week, much of it, on doubt, due to ... responsibilities. Interestingly, when there are three or more children in the household, men are found to work even fewer hours around the house than before the appearance ofthe third child. Whereas women's ... rise to approximately 56 per week, the corresponding figure for men, 16, actually represents a decrease. The data suggest that the increased presence of women in the paid work force has yet to...
... product, then adjustments will have to be made to the product's design. Similarly, market research, which examines the extent and nature ofthe demand for products, has the role of guiding ... not only the result of product research, but is also influenced by testing and market research. If the testing stage (after assembly and inspection) reveals unacceptable problems in the finished ... product sales, also serves to foster future sales by devising suitable advertising for the goods. Thus the reality of consumer goods manufacturing goes well beyond a simple linear production process....
... contributed to the embryo, the resultant offspring will be chimeric (up to ca. 50% of animals). The ESC contribution to a mouse can high (up to ca. 80% ofthe cells), and will often include the germline ... been the notion ofthe existence of ‘inhibitory’ factors (IFs) associated with sperm cells. These IFs are envisaged to prevent exogenous DNA uptake so as to protect the genetic integrity ofthe ... variety of routes including pronuclear microinjection and SMGT [74, 75, 76]. In the context of human germline gene therapy, such vectors offer the potential advantage of eliminating the threat...