localization and anchorage of maternal mrnas to cortical structures of ascidian eggs and embryos using high resolution in situ hybridization
Báo cáo hóa học: " Multisensor Processing Algorithms for Underwater Dipole Localization and Tracking Using MEMS Artificial " ppt
... Professor with major appointment in the Electrical and Computer Engineering Department and minor appointment in the Mechanical and Industrial Engineering Department In 2003, he was promoted to ... Professor in the Electrical and Computer Engineering Department, the Coordinated Science Laboratory, and the Beckman Institute He was on sabbatical leave at the University of Washington in Spring ... the training approach, accuracy at the fringes is low and distorts the overall performance of points within the scope of the array We have shown the ability to localize a dipole source using an...
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Báo cáo khoa học: "Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer" ppsx
... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... rate according to IHC and 1+ was defined as the number of discrepant IHC and 1+ cases divided by the total number of IHC and 1+ cases and was 19.2% (5/ 26) Following the same way of counting, the ... 10-minute changes of xylene, transferred through two 3-minute changes of 100% ethanol, one 3-minute changes of 85% ethanol, one 3-minute changes of 70% ethanol and immersed for 15 minutes in pure...
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Báo cáo y học: "Experimental annotation of the human pathogen Candida albicans coding and noncoding transcribed regions using high-resolution tiling arrays" doc
... transcriptase (Invitrogen) Actinomycin D was used to inhibit synthesis of the second cDNA strand to a final concentration of μg/ml Page 10 of 14 To assess actinomycin D efficiency in resolving spurious ... actinomycin D In light of their finding, we have used actinomycin D to prevent the appearance of these artifacts Indeed, as shown in Figure 3a, b, the use of actinomycin D reduced, in part, the ... growth, we defined differentially expressed intergenic transfrags in C albicans cells growing in the caecum as well as in cells undergoing hyphal and biofilm growth Using a stringent cutoff (see Materials...
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Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization" potx
... percentage of grains on pig chromosome is increased from 31% to 86% and the region is reduced from (q2.2 - q2.7) to one band q2.5 The number of grains pointed in this region is so high, that ... chromosome 12 in the rabbit (Oryctolagus cunicudus L.) by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically ... with the use of high- resolution chromosome spreads, will be useful in several fields: localization of unique sequences, increasing the precision of gene localization on chromosomes (Lin et al.,...
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Applying blog to motivate the students of class 11a4 at quynh tho high school in studying writing skill
... according to certain convention: to form words and words have to be arranged to from sentences Writing involves the encoding of a message of same kind that is translated into language According to ... applying blog in improving our English writing skills And, blog brings to us many advantages such as: motivating us in learning writing skills and approaching using technical English through using ... The feeling of the students about using blog in writing Figure 6: The student attitudes towards using peer feedback to complete writing 4.1 The attitude of students before using blog Findings...
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Pre writing activities used to teach 12 th form students at hong linh high school in english writing lessons
... to the writing topics in order to create the students’ interest According to them, by brainstorming some information to the writing topics, their students find it easier to understand and they ... activities such as: Brainstorming about people, places, and feelings, talking and listening in pairs or groups, reading and listing ideas and information are most frequently used by lots of the teachers ... PRE-WRITING ACTIVITIES APPLIED TO TEACH ENGLISH WRITING LESSONS TO THE 12TH FORM STUDENTS AT HONGLINH HIGH SCHOOL 4.1 Findings 4.1.1 The role of using Pre-writing activities The findings of this...
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Báo cáo khoa học: "Cohesion and Collocation: Using Context Vectors in Text Segmentation" pptx
... over the input text, and at pre-determined intervals (every 10 words), the vectors associated with the words in each window are added up, and the cosine between the resulting window vectors is ... can again be compared against each other and word vectors If the word vectors in two adjacent portions of text are added up, then the cosine between the two resulting vectors is a measure of the ... 3.2 ,,w, Comparing Window Vectors (1) In order to represent pieces of text larger than single words, the vectors of the constituent words are added up This yields new vectors in the same space,...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
... aimed to explore, using in situ hybridization on a high throughput platform, possible prognostic roles by miR-155 in all NSCLC cases and subgroups according to histology and stage Patients and ... contributed in the clinical and demographic database and KAS and SAS in making the TMAs RB and LTB supervised and participated in the study design, result interpretation and in the writing All authors ... used to scan the TMA slides of ISH staining The slides were loaded in the automated loader (Applied Imaging SL 50) and specimens were scanned at low (1.25×) and high resolution (20×) using the...
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Báo cáo hóa học: " Landmine Detection and Discrimination Using High-Pressure Waterjets" potx
... chosen training and test sets, using 80% of the data for training and the remaining 20% for test (see following sections) K-means clustering [15] of the landmine encounters from the training data ... is to show the potential of using the sound produced by a high- pressure waterjet impact to detect and classify buried landmines Three methods of detecting and classifying a buried object using ... an M.S and Doctor of Science degree in electrical engineering from Washington University in St Louis in 1994 and 1997, respectively He conducts research on a wide range of topics including electrocardiology,...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, ... increasing sensitivity as compared with hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization protocol...
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báo cáo khoa học: "mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization" doc
... operated in solution [15-19] An AFM probe is inserted into the living cells to extract mRNAs Obtained mRNAs are subjected to RT-PCR and then to nested PCR or quantitative PCR Since the AFM has high ... β-actin mRNAs were detected mainly in the vicinity of the nucleus Usually β-actin mRNA is known to exist mainly in the vicinity of the nucleus, and these results agreed with the results of our ... performed using µl of RT-PCR reaction buffer as a template The amounts of initial β-actin mRNA were determined by a standard curve built using β-actin cDNA Figure shows some examples of ISH and the...
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Improve of produced gas quality by using air steam in fluidized bed gasifier
... from 1450 to 853 ( ( - ) by using steam and air mixture at ) in the same range of increasing temperature And this due to: Steam converts high molecular weight hydrocarbons of tar into smaller ... the fluidizing agent and introduced into the reactor Air was supplied by a compressor and was heated to 200 °C in a preheater The steam of 150 °C was produced in a steam generator and its mass ... From Figures to 11, it can be observed, that the increasing in values of ratio of steam to biomass lead to increases each of hydrogen and methane, carbon dioxide, gas yield, low heating value,...
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Detection of low grade mosaicism in turner syndrome using fluorescence in situ hybridization
... achieved using the fluorescence in- situ hybridization (FISH) technique in addition to the standard cytogenetic techniques vi Results showed a low percentage of abnormal X cells (45,X and 47,XXX) in ... G-banding and FISH The remaining 12% (2 out of 17) showed a low percent ranging from 0.06% to 0.1% of 46, XX cells only with FISH technique Two cases remained apparent 45,X Turner with G-banding, ... 15 Summary and percentage of occurrence of some of the physical findings in Turner syndrome Table 3: PAGE 51 Summary of FISH results of Turner patients with a 45,X cell line by G-banding 54 Table...
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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf
... gel, electroeluted and cloned to be sequenced and further digested and subcloned into pBluescriptII KS(+) vector (Stratagene) To determine the sequences of the 5¢ and 3¢ ends of intron 1, we performed ... blotting using an internal primer as a probe Strong expression of Muc4 is shown in trachea, duodenum and intestine, while a much weaker expression is shown in stomach In each case, the quality of ... found in various proteins This domain is a part of the nidogen glycoproteins, which are expressed by mesenchymal and epithelial cells Nidogens have a high affinity for lamininbinding protein and...
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An analysis of errors made by vietnamese secondary school students in using english modal auxiliary verbs can, could, may, must and semi auxiliary verb have to
... expressions of their feelings and opinions of their feelings and opinions in order to reflect cooperation in conversations These aspects of language belong to what is referred to as modality.Accodding to ... the teaching and learning of language in general and English in particular In Errors in language learning and use", Carl James defined: Error analysis is the process of determining the incidence, ... students make in using can, could, may, have to and must? The results from the study show that students have a lot of difficulties in understanding the meaning of modal verbs in using modality of possibility...
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt
... Levels of MIH and CHH in the sinus gland during the moult cycle As individual SG from single crabs always contained almost identical profiles and quantities of peptides (preliminary experiments using ... that during premoult, increases in cGMP levels following a 30-min incubation in peptide were dramatically blunted during premoult, and that competence to respond to peptide was restored in late ... Furthermore, in experiments using CHH as the saturating ligand, a parallel increase in receptor number during postmoult was not observed Thus, during postmoult, a significant recruitment of receptors...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... kinase Pim-1 induces translocation of the TRAF4-interacting protein-sorting Nexin 6–TRAF4–associated factor from the cytoplasm to the nucleus [19] Thus, it will be interesting to see in the future ... TRAF domain of TRAF2 and TRAF3 but were detected regularly in cells transfected with TRAF2/3 deletion mutants consisting of the RING–zinc finger domain Since TRAF proteins tend to form homo- and/ or ... necrosis factor (TNF) receptor-associated factor gene (TRAF1) is up-regulated by cytokines of the TNF ligand family and modulates TNF-induced activation of NF-jB and c-Jun N-terminal kinase J Biol...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... acylation and, thereby, be involved in membrane anchoring of the pilin Since PilA4 is similar to the meningococcal pilin and contains several central serine residues, it is tempting to speculate ... amounts in the inner and outer membranes The presence of PilQ in inner membranes is interesting, because secretin-like proteins of type IV pili and type II protein translocation machineries are ... implicated in retraction of the PilA4-comprising DNA translocator transporting the DNA through the periplasmic space Binding of the DNA to the DNA-binding protein ComEA on the surface of the inner...
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