lipids lipoproteins and atherosclerosis
Báo cáo Y học: The amyloid precursor protein interacts with neutral lipids Liposomes and monolayer studies pdf
... molecules When APP concentrations were 0.35 and lgÆmL)1, s1 reached 200 and 23 min, while s2 was around 25 and 75 min, respectively From these results, s1 and s2 appear to be very sensitive to the ... of an electrostatic repulsion effect between lipids and the protein at physiological pH Anionic lipids, which represent % 20% of biological membrane lipids, provide either a source of electrostatic ... of the 30% layer and samples were centrifuged at 160 000 g for h at °C Fractions (0.5 mL) from the gradient were subsequently collected from the bottom of the tube and the lipid and protein contents...
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Báo cáo y học: "Biomarkers of endothelial dysfunction, cardiovascular risk factors and atherosclerosis in rheumatoid arthritis" pptx
... Switzerland) The intra-assay and inter-assay coefficients of variation (respectively) were 5.1% and 8.6% for IL-1, 3.4% and 5.2% for IL-6, 6.9% and 7.4% for TNF-α, 3.1% and 5.2% for VCAM-1, 4.1% and ... IL-6 and tumor necrosis factor [TNF]-α), cytokine suppressant therapy (disease-modifying antirheumatic drug [DMARD] and prednisone use) and biomarkers of endothelial dysfunction (Tables and 2) ... 08:00 and 10:00 hours All laboratory analyses except for assessments of cytokines and biomarkers of endothelial dysfunction were performed within hours of sampling These comprised lipids, hs-CRP and...
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Báo cáo y học: "Inflammation, carotid intima-media thickness and atherosclerosis in rheumatoid arthritis" doc
... function and structure in RA It is fundamental to examine the interrelationships between the high-grade but variable systemic inflammation that characterises RA and the potentially intermittent and ... intermittent and reversible insults to vascular function and structure – to prove whether they indeed reflect accelerated atherosclerosis – and to assess their long-term significance in terms of ... Arthritis Research & Therapy Vol 10 No Veldhuijzen van Zanten and Kitas Another noninvasive technique used as a surrogate for atherosclerosis involves the assessment of vasodilatory responses:...
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Báo cáo y học: "Metabonomics-based Omics Study and Atherosclerosis" ppsx
... tools and personnel [1] Atherosclerosis is one of the leading causes responsible for cardiovascular morbidity and mortality, a complicated and multifactorial disease associated with genotypes and ... diet, environment and lifestyle Key words：Metabonomics, metabolomics, atherosclerosis, metabolic disturbances, ： inflammation Introduction Living systems are dynamic and complex, and their behavior ... :xiangdong.wang@telia.com Abstract Atherosclerosis results from dyslipidemia and systemic inflammation, associated with the strong metabolism and interaction between diet and disease Strategies based...
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Lipids, Fats, and Oils
... for coloring and flavoring foods, and making red (carthamin) and yellow dyes.43 Safflower oil is flavorless and colorless, and is nutritional It is used mainly as a cooking oil and for the production ... manufacturers and wholesalers of any edible oil product and prescribing the form, terms, and conditions thereof and the fees to be paid therefore, and providing for the renewal, suspension, and cancellation ... 5.2 MAJOR OILS AND FATS All oils and fats, with their high carbon and hydrogen content, can be traced back to organic sources Oils and fats are also produced by plants, animals, and other organisms...
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Effectiveness of PRECEDE model for health education on changes and level of control of HbA1c, blood pressure, lipids, and body mass index in patients with type 2 diabetes mellitus docx
... (0 and at month 1, were baseline visits; and to 9, were follow-up visits, every months) Baseline data were collected during visits and 1, and during visits to assigned models were applied and ... study and participated in its design and perfomed the statistical analysis and drafted the manuscript FJAB, JCAH, CBL drafted the manuscript and made substantial contributions to the analysis and ... participate; 13 refused, and PCC were randomized in two arms: Precede Health Promotion Education (PHPE) and Conventional Health Promotion Education (CHPE) The participants at each PCC were randomized by...
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FOOD LIPIDS Chemistry, Nutrition, and Biotechnology ppt
... different components, usually an alcohol and an acid Complex lipids include glycerophospholipids (phospholipids), glyceroglycolipids (glycolipids), and sphingolipids These structures yield three or ... 2-, and 3-series prostaglandins originating from 20:3v6, 20:4v6, and 20:5v3, respectively The structures of the 1-, 2-, and 3-prostaglandins differ Akoh/Food Lipids: Chemistry, Nutrition, and ... parts: Chemistry and Properties; Processing; Oxidation and Antioxidants; Nutrition; and Biotechnology and Biochemistry We are grateful to the readers and users of the previous editions and can only...
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Lipoproteins – Role in Health and Diseases by Saša Frank and Gerhard Kostner doc
... Apolipoproteins in Alzheimer's Disease 613 Etsuro Matsubara Section Lipoproteins and Cancer 621 Chapter 26 Lipoproteins and Cancer 623 Caryl J Antalis and Kimberly K Buhman Chapter 27 Role of Lipoproteins ... lipid:protein complexes, i.e lipoproteins Lipoproteins consist of a lipid core of triglycerides and cholesteryl esters, and an amphiphilic surface of free cholesterol and phospholipids Apolipoproteins (apo-Lp) ... low density lipoproteins, low density lipoproteins (LDL) and high density lipoproteins (HDL) - and yet each of them may be divided into numerous subfractions The major function of lipoproteins...
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FOOD LIPIDS Chemistry, Nutrition, and Biotechnology pdf
... different components, usually an alcohol and an acid Complex lipids include glycerophospholipids (phospholipids), glyceroglycolipids (glycolipids), and sphingolipids These structures yield three or ... 2-, and 3-series prostaglandins originating from 20:3v6, 20:4v6, and 20:5v3, respectively The structures of the 1-, 2-, and 3-prostaglandins differ Akoh/Food Lipids: Chemistry, Nutrition, and ... parts: Chemistry and Properties; Processing; Oxidation and Antioxidants; Nutrition; and Biotechnology and Biochemistry We are grateful to the readers and users of the previous editions and can only...
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Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
... E coli phospholipids and 0.26 lgÆmL)1 mLolB for the indicated times The reaction mixtures were fractionated into pellet (p) and supernatant (s) fractions and analysed by SDS–PAGE and immunoblotting ... Escherichia coli phospholipids were obtained from Avanti Polar Lipids (Alabaster, AL, USA) and were washed with acetone as reported previously [14] Synthetic phospholipids, CL, PG and PE, containing ... preference and caused incorporation of Lpp into both the outer and inner membranes We then examined the mLolB-dependent localization of lipoproteins to liposomes prepared from E coli phospholipids...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... electrophoresis and the spectrophotometrically determined A260 A280 ratio veried that the pDNA was of high quality and purity Lipid dispersions and lipoplexes Cationic lipids, DOPE and cholesterol ... collapsed at 49 mNặm)1 and 39 A2 At 37 C, the two-dimensional transition was absent and only a liquid-expanded state was evident For all derivatives, molecular dimensions increased and compression forces ... midpoint, the all liquid-expanded states at 23 C and 37 C of the dipalmitoyl and distearoyl derivatives, respectively, in monolayers complement the nature of these lipids in bilayer assemblies...
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Nutraceutical and Specialty Lipids and their Co-Products doc
... nutrition, and health aspects of specialty fats and oils and their co-products and to address stability issues and their potential application and delivery in functional foods and geriatric and other ... Nutraceutical and Specialty Lipids and their Co-Products FIGURE 1.2 Chemical structures of the major phospholipids lipids in living organisms The acyl groups in phospholipids occur in the sn-1 and sn-2 ... his outstanding contributions to food lipids and flavor chemistry, and was also recognized by IFT as a Fellow in 2005 Dr Shahidi is a founding member and a past chair of the Nutraceutical and Functional...
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Báo cáo khoa học: Structural identification of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox) pptx
... glycerol monoethers and diethers, and mixed glycerol ether ⁄ esters In addition, glycerol diethers and ether ⁄ esters were identified with one ladderane moiety and one alkyl chain and even glycerol ... an internal standard (6,6-d2)3-methyleicosane), silylated with BSTFA and analysed by GC An internal standard was added to an aliquot of the acetone-fraction and analysed by GC and GC ⁄ MS after ... at 60 °C for 15 and all fractions were analysed by GC and GC ⁄ MS Isolation of ladderane lipids For isolation of lipids the extract (54 mg) of a large batch (45 g dry weight) of Candidatus ‘B anammoxidans’...
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Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids potx
... and transfer lipids is demonstrated in the present study using both natural and fluorescently labeled ligands Results Molecular model comparison Previous molecular modeling analysis of Par j and ... fluorescence was monitored with excitation and emission wavelengths at 275 and 310 nm, with and nm bandwidth, respectively Buffer contributions were corrected and inner filter effect was negligible, ... j and substituted by a lysine in Par j 2, whereas Tyr81 is absent in both Parietaria sequences The cavity of maize ns-LTP is highly polarized and mainly hydrophobic on one side, and polar and...
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Báo cáo khoa học: Glycation and glycoxidation of low-density lipoproteins by glucose and low-molecular mass aldehydes Formation of modified and oxidized particles pot
... artefactual oxidation during such sample handling and analysis [23,24] Samples (80 lL) were removed from the LDL incubations and 0.1 vol each of 20 mM EDTA and 0.2 mM BHT and 670 lL borate buffer (pH > ... 205 and 234 nm and a RF-10AXL fluorescence detector (for TOH; kex 290 nm and kem Ó FEBS 2003 3574 H M Knott et al (Eur J Biochem 270) 330 nm) Metabolites were quantified by comparison with standards ... incubations and loaded onto precast 1% (w/v) agarose gels Native and acetylated LDL (Ac-LDL, prepared as described previously [25]) were loaded as negative and positive controls, respectively, and the...
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atherosclerosis, experimental methods and protocols
... Fellowship References Davis, R A and Vance, J E (1996) Structure, assembly and secretion of lipoproteins, in Biochemistry of Lipids, Lipoproteins and Membranes (Vance, D E and Vance, J E., eds.), Elsevier, ... function, in Lipoproteins and Lipids (Durrington, P N.,ed.), Wright, London, pp 255–277 Davis, R A (1991) Lipoprotein structure and secretion, in Biochemistry of Lipids, Lipoproteins, and Membranes ... surface monolayer of amphipathic lipids (phospholipids and unesterified cholesterol) and specific proteins called apolipoproteins A number of different phospholipids are incorporated into the...
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Báo cáo y học: "Practical and theoretical barriers to the prevention of accelerated atherosclerosis in systemic lupus erythematosus" docx
... to participate are relatively young and healthy, with very mild SLE, and few other health problems or medications Among those patients with more long-standing and severe SLE, reasons for non-participation ... understanding of patient social and cultural factors will be essential to achieving acceptance of lifestyle changes and medications Progress will only be made as basic science brings an understanding ... understanding of the mechanisms of accelerated atherosclerosis in SLE and as clinical studies address both the efficacy and the effectiveness of existing and improved risk factor control 13 14 15...
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Báo cáo y học: "Activation of transforming growth factor-β1 and early atherosclerosis in systemic lupus erythematosus" pptx
... the data and helped to draft the manuscript YA obtained consent from patients and collected the samples and patient data for the study, and participated in the coordination of the study and writing ... patients and control individuals, and examined whether TGF-β1 activation was associated with clinical phenotype, in particular inflammatory disease activity, cumulative organ damage and early atherosclerosis ... double stranded (ds)DNA and anticardiolipin (ACL) were measured ANAs were measured by indirect immunofluorescence on Hep2 cells Antibodies to dsDNA (IgG) and cardiolipin (ACL; IgG and IgM) were...
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Báo cáo y học: " Early atherosclerosis in systemic sclerosis and its relation to disease or traditional risk factors" pdf
... using IMT of the carotid artery as a marker of early atherosclerosis Lekakis and colleagues [22], Kaloudi and colleagues [23], and Bartoli and colleagues [24,25] found strongly increased IMT values ... Kaloudi and colleagues [23] found no significant differences between mean IMT between subtypes On the other hand, our results are in agreement with those of Cheng and colleagues [26,27] and Szucs and ... groups in studies by Kaloudi and colleagues [23] and Bartoli and colleagues [24,25] are from overlapping cohorts since these studies were performed in the same center and published in the same period...
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Báo cáo y học: "Altered lipoprotein metabolism in chronic inflammatory states: proinflammatory high-density lipoprotein and accelerated atherosclerosis in systemic lupus erythematosus and rheumatoid arthritis" ppsx
... accelerated atherosclerosis High-density lipoproteins: characteristics, synthesis, degradation, and mechanisms by which normal high-density lipoproteins protect from atherosclerosis Low-density lipoproteins: ... composed of lipoproteins (apoA1 being the most abundant), phospholipids, and cholesterol esters Cholesterol esters are shuttled to apoB-rich low-density lipoproteins (LDLs) and very-low-density lipoproteins ... apoA1 and others both apoA-I and apoA-II [14] In general, small dense HDLs are lipid-poor and protein-rich discs, but the majority of HDL particles are spherical and rich in both lipid and protein...
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