lines culture media and expression vectors for hek293 cells and descendants
Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx
... generation of RNAi for pSM155 and pSM30, we examined the knockdown of expression of firefly Luciferase (Luc) HeLa cells were transfected with the parental and modified vectors containing control and Luc targeting ... codex.cshl.edu (for miR30 system), and the published guidelines for selecting highly effective siRNA sequences [20] The oilgos for candidate sequences also contained cohesive ends for our simplified ... pSM155 and pSM30 vectors improve the expression of marker proteins in artificial miRNA-expressing cells All miR155 and miR30 constructs contain an EGFP marker as illustrated in Fig (A) HeLa cells...
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Báo cáo sinh học: " Recombination-ready Sindbis replicon expression vectors for transgene expression" pdf
... mammalian and insect cells to plastic culture plates, allowing cells insect cells to be transiently co-cultured with BHK cells producing PIPs BHK cells were transfected with pBG78 and pBG44 as ... transfected BHK cells were washed with complete L-15 or Schneider's media and × 106 m and C6/36 or Drosophila S2 cells were added directly onto the BHK cells The co-cultures were incubated at 28°C for 24 ... C6/36 cells (data not shown) Therefore, the replicons are transferred from the BHK cells to insect cells, and the co -culture assay is able to rapidly establish stable cell lines We used our co-culture...
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Báo cáo hóa học: " Recombination-ready Sindbis replicon expression vectors for transgene expression" doc
... mammalian and insect cells to plastic culture plates, allowing cells insect cells to be transiently co-cultured with BHK cells producing PIPs BHK cells were transfected with pBG78 and pBG44 as ... transfected BHK cells were washed with complete L-15 or Schneider's media and × 106 m and C6/36 or Drosophila S2 cells were added directly onto the BHK cells The co-cultures were incubated at 28°C for 24 ... C6/36 cells (data not shown) Therefore, the replicons are transferred from the BHK cells to insect cells, and the co -culture assay is able to rapidly establish stable cell lines We used our co-culture...
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Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx
... duodenum cells the expression from both W2 and V2 exceeded the G1 and H1 expression In the lung cells the V2 showed similar level of expression as the G1 and H1 but W2 slightly higher expression ... extent in skin cells and poorly in duodenum and kidney cells (Figure 3) The vectors W1 and V1 not have a Kozak consensus sequence and were expressed to a low level in cells from lung and to a very ... in all four horse cell lines as compared to the parent vectors W1 and V1 In the skin and kidney cells the expression from W2 and V2 reached similar levels to that of G1 and H1 that have a semi...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... Cell culture, transfection and cell lysis Human transformed primary embryonal kidney cell lines HEK293 and HEK293sw (a kind gift from D Selkoe, Harvard Medical School, Boston, MA, USA) were cultured ... electrophoresis and stained with ethidium bromide For experiments performed in coamplification conditions two pairs of primers P1BACE and P2BACE, for BACE mRNA (20 lM), and P1GAPDH and P2GAPDH, for the ... the ribozyme to its target site Expression of ribozyme plasmids in mammalian cells Three cultured cell lines HEK293, HEK293sw and IMR-32 were used to study the expression of ribozymes, which were...
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Model Policy Guidelines for the Appropriate Use of Social Media and Social Networking in Medical Practice ppt
... the Special Committee on Ethics and Professionalism who provided input and direction for this project Model Guidelines for the Appropriate Use of Social Media and Social Networking in Medical ... have developed social media policies.8,9,10,11 Social media has enormous potential for both physicians and their patients It can be used to disseminate information and forge meaningful professional ... technology and societal trends will continue to change the landscape of social media and social networking, and how these websites are used by patients and physicians will evolve overtime These guidelines...
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Chapter 2Communicating Over the Network Quangkien@gmail.com.OverviewDescribe the structure of a network, including the devices and media that are necessary for successful communications. Explain the function of protocols in network communications. Ex potx
... rules for: (4) Format Accessing the media Error detection Setup and termination 30 Protocol Suites and Standards Early days – proprietary network equipment and protocols Now – Industry standards ... network media have different features and benefits Criteria for choosing a network media are: – The distance the media can successfully carry a signal – The environment in which the media is ... Servers and Modems – Security Devices (firewalls) 14 Intermediary Devices and their Role on the Network Processes running on the intermediary network devices perform these functions: – Regenerate and...
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nonviral vectors for gene therapy, methods and protocols
... plasmid DNA solution Stand for 15 at room temperature 3.3 Gene Transfer Protocol into Cultured Cells Plate cells in 24-well tissue culture dishes ( 16 mm) at × 105 cells/ well and grow overnight in ... major band of closed circular DNA and minor amount (
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interactive design for new media and the web 2001)
... maps and flowcharts and the writing of design documents We will see how the latest forms of digital media can now be applied to entertainment, games, information systems, and education And finally, ... updated and has the media enhancements of video, audio, and full-color graphics As a result, it is a perfect resource for anyone who has a constant need for information People with that need (and ... that promoted and popularized Britannica’s position as the world’s foremost authority on everything, Britannica expanded its news and pop -culture offerings and became a free information site...
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báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt
... tubulin alpha and beta involved in the assembly of microtubules and intermediary filaments, as well as keratin and 15 We also looked for genes involved in cell adhesion and migration and found integrin ... (overexpression in AC vs NT, SCC vs NT and SCLC vs NT was 2.2, 2.1 and 3.2-fold, respectively); collagen, type I, alpha (overexpression in AC vs NT, SCC vs NT and SCLC vs NT was 7.98, 3.24 and ... cell cycle, the cyclines A2 and B2, cyclin-dependent kinase and the cell division cycle genes and cyclin E The expression patterns of genes of the centromer/kinetochore complex and genes involved...
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báo cáo hóa học: " Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD" pptx
... characterized [26] For some experiments, primary murine microglia were also used and were prepared from mixed glial cultures from day old postnatal pups in a standard fashion [27] Cells from both cultures ... 2006, 3:27 vate; Invitrogen) Cells were then allowed to adapt to the serum-free media for 24 hours before starting the experiments The stimulants used for the cell culture experiments included ... of microglia with IL4 or IL-13 BV2 cells were treated with IL-4 or IL-13 for 24 hrs and the mRNA expression levels for AG1 (Panel A); MRC1 (Panel B); FIZZ and YM1 (Panel C) were determined using...
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Báo cáo khoa học: " Cassette deletion in multiple shRNA lentiviral vectors for HIV-1 and its impact on treatment success" potx
... expanded populations for 2.2, 5.3 and 6.3, but only 83 populations for 3.2, and 48 for 4.3 Approximately 10 - 12 weeks after transduction the populations were selected and sufficiently expanded ... Groneman formerly of JJR for preparing the constructs, Li Wang formerly of JJR for technical assistance, and Emer Prof Donald Birkett, Dr Gregory Arndt and Dr Laurent Rivory formerly of JJR for helpful ... (Stratagene), and H2O to a final volume of 50 μl Each PCR was cycled at 1×: 95°C for min., 35×: 95°C for 20 sec | 66°C for 20 sec | 72°C for 0.5 - (depending upon template length), and 1× 72°C for Samples...
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Báo cáo sinh học: "The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery" pptx
... target for gene transfer) but not in mature cells (80) Therefore, it will be accessible for proviral integration during the transduction process and its continuing expression in maturing T cells ... defective vectors for transfer of heterologous gene sequences The proviral form, being DNA, can be readily isolated in standard plasmid cloning vectors Page of 13 (page number not for citation ... virus (EIAV) [58] and maedi/visna virus (MVV) [59] For CAEV and MMV viral infection of macrophages seems to induce an inflammatory response involving macrophages and CD4+ and CD8+ T cells It is this...
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Social Media and Internet-Based Data in Global Systems for Public Health Surveillance A Systematic Review
... InSTEDD, MedISys, MiTAP, and Proteus-BIO), and systems collect from both formal members and informal sources (EWRS and GOARN) ProMED-mail is the only system obtaining firsthand information from its ... feeds from thousands of news sources from around the world, and many systems deal with a huge amount of information each day MediSys, for Social Media and Internet-Based Data for Public Health ... (mark or catalog) the information for future use, and to comment on the information (for sharing and collaboration with other scientists), which can also be used to inform machine-learning algorithms...
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Using Social Media and Internet Data for Public Health Surveillance The Importance of Talking
... resulting in too much information; (3) algorithms and statistical baselines are not well developed; and (4) new information related to Using Social Media and Internet Data for Public Health 37 health ... information is not always moderated by professionals or interpreted for relevance before it is disseminated to interested surveillance epidemiologists; (2) there is no standardized system for ... makes the article by Edward Velasco and his coworkers in this issue of the Quarterly so valuable and timely Velasco and his colleagues systematically searched for and reviewed more than 20 years...
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Some suggestions to improve speaking skill for the second year students at thanh hoa university of culture, sports and tourism (TUCST)
... time and make it most clearly to understand and remember The students listen to the teacher’s presentation and try their best to understand and memory In this stage, the students only listen and ... in teaching and learning speaking skill that teachers and non-English major students encountered at Thanh Hoa University of Culture, Sports and Tourism and give some suggestions for developing ... conferences and competitions use English For example; miss World contest and World cup Now Internet and mobile phone are very popular to contact people from all the world and the language for them...
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Using biological networks and gene expression profiles for the analysis of diseases
... have expedited and resulted in many ideas in this thesis I thank Prof Choi Kwok Pui, for his insights and knowledge in statistics I thank Prof Ken Sung and Prof Thiagu, for reading and listening ... algorithm is used for the weighted-average computation above, which basically assigns bigger weights for values close to the median and smaller weights for values far from the median, so that the ... distribution with mean µ and standard deviation σ and real signal follows an exponential distribution with parameter alpha α This formulation results in a closed form solution for the expected real...
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Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... transfection, and is therefore the standard form of PEI for gene delivery Highly branched polymers such as the 25 kDa and the 800 kDa molecular weight PEI polymers are most frequently used for gene ... transgene expression from AAV vectors seems to be dependent of the brain area and the presence of receptors for AAV uptake on target cells AAV-based vectors can produce high levels of transgene expression ... to dividing cells, although they are well suited for on-site delivery to neural precursors for lineage studies (Cepko et al., 2000), to tumor cells for therapeutic intervention, and for ex vivo...
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Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system
... normal culture medium for the cells was restored Cells were further cultured for various periods of time indicated before collected for luciferase activity measurement After the incubation, cells ... or cells, and the level and duration of the expression can be regulated as desired With currently available promoters and gene delivery vectors, it remains a great challenge to temporally and ... type (AAV-2) and baculovirus vectors to drive in vitro and in vivo transgene expression A high level, neuron-specific and long-term transgene expression was achieved in rat striatum and substantia...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
... of cells for gene transfer and expression GFP can be used as the reporter in gene transfer and expression experiments, in which cells expressing GFP are sorted using flow cytometry and expanded ... Ng cDNA Digestion with HindIII and Digestion with HindIII and BamHI and purification BamHI and purification Ligation and screening for positive clones Sequencing for verificaiton Fig.7 Construction ... physiological model for learning and memory and Ng knockout mice displayed impairment in spatial learning and hippocampal long-term and short-term plasticity Evidences show Ng expression is developmentally...
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