... ultrasonicator at °C Cells were sonicated (4 · 15 s with 20 s intervals) in sample buffer [100 mm phosphate buffer (pH 7.5), 20 mm EDTA (pH 8.0), 10% (v ⁄ v) acetone] Triton X-100 was added to a final ... concentrator before HPLC analysis HPLC separation of desferrioxamines was performed on a reverse-phase column (Nucleosil C18, lm, 4.6 by 150 mm) with 150 lL injection volume and mLÆmin)1 flow rate ... expression system (Amersham Biosciences) Purification of the GST hybrid protein in glutathione–Sepharose columns was performed according to the manufacturer’s instructions After elution of glutathione...
... Fortunately—and this, more than anything, is what I think makes Amusing Ourselves to Death so emphatically relevant—my father asked such good questions that they can be asked of nontelevision ... speech, it is recreated anew by every medium of communication—from painting to hieroglyphs to the alphabet to television Each medium, like language itself, makes possible a unique mode of discourse ... included another Commandment: Thou shalt not make mechanical representations of time That the alphabet introduced a new form of conversation between man and man is by now a commonplace among scholars...
... aspects of social marketing [17] to promote evidence-based therapies rather than brand-specific pharmaceuticals These interventions could be a critical component of implementing guideline-concordant ... patient activation interventions can provide The results from this paper and the parent study emphasize that most patients in the study were willing and interested in taking a proactive approach ... process still brought in the letter to have the conversation with their provider Therefore, the emphasis on bidirectional interaction is not only about patients who want to be involved in the decision-making...
... aspects of social marketing [17] to promote evidence-based therapies rather than brand-specific pharmaceuticals These interventions could be a critical component of implementing guideline-concordant ... patient activation interventions can provide The results from this paper and the parent study emphasize that most patients in the study were willing and interested in taking a proactive approach ... process still brought in the letter to have the conversation with their provider Therefore, the emphasis on bidirectional interaction is not only about patients who want to be involved in the decision-making...
... (intervention modelling) These respectively correspond to the theoretical phase and the modelling and exploratory trial phases of the UK Medical Research Council (MRC) Framework for the development ... is a consistent finding that changing clinical practice is unpredictable and can be a slow and haphazard process Over the last decade a considerable body of literature has been published suggesting...
... oropharyngeal and tracheal samples taken at inclusion The same species were identified in samples from both the oropharynx and the trachea of six Lp patients and three controls Subsequent oropharyngeal ... in the oropharynx and to prevent VAP [11,12] Aspiration of pathogenic bacteria constitutes the main cause of VAP, and thus reducing the occurrence of such microorganisms in the oropharynx should ... antibiotic-resistant strains In humans, lactobacilli colonise the oropharynx soon after birth, and thereafter constitute part of the normal oropharyngeal flora and, accordingly, these bacteria will enter...
... experiments, (ii) on different days, while, lastly, (iii) the cells were harvested in a growth phase in which small changes in culture optical density will result in relatively large differences ... highest ratio and signal CVs (additional Table 2): (i) are of unknown function, (ii) are (pro) phage-derived, (iii) encode proteins involved in transport of various compounds, or (iv) encode ... they were obtained at challenging conditions: (i) flask-grown cells, (ii) harvesting in a growth phase in which relatively large changes in gene-expressions occur, and (iii) change of factors (e.g...
... THE IELTS EXAMINATION (Nghiên cứu việc sử dụng từ nối việc phát triển kỹ nói viết phục vụ loại hình thi IELTS) M.A MINOR PROGRAMME THESIS Field: English Linguistics Code: 60.22.15 Supervisor: ... designed to test the full range of formal language skills required for academic purposes, but emphasize basic survival skills in a broad social and educational context General Training is suitable...
... Dựng Công Trình Đô Thị ) M.A Minor Programme Thesis Field: Methodology Code: 60 14 10 Supervisor: Pham Minh Hien, M.A Hanoi - 2011 TABLE OF CONTENTS STATEMENT OF AUTHORSHIP I ACKOWLEDGEMENTS...
... include components of a Speaking Exercise, Teaching Interactional Speaking and Free Discussion He emphasis that the social and cultural factors need to be taken into teaching and learning speaking ... spelling book before the pupils and with a penknife pointed, one by one, to the letters of the alphabet saying, "What's that?" (Edward and Richey, p.172) As education extended beyond society's ... transmit basic information 16 clearly In the early part of the twentieth century, however, this emphasis started to shift John Dewey and his disciples of Progressive education left a legacy of studentcentered...
... (Ebioscience, San Diego, CA, USA), at 4°C for 30 minutes, washed twice with cold 2% newborn calf serum/phosphate-buffered saline (NCS/PBS) buffer and fixed overnight in 4% paraformaldehyde The cells were ... oxide and subsequent cartilage destruction [39] IL-4 may also have suppressive effects on macrophage proliferation [40] and cytokine production [41] Conclusions The data in the present study ... metalloproteinase; NCS: newborn calf serum; PBMC: peripheral blood mononuclear cells; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PMA: phorbol myristate acetate; RA: rheumatoid...
... boating and elephant riding (half a day) Destinations:Depart from Da Lat, get on cable hanger to Truc Lam Temple, go boating on Tuyen Lam Lake across the camping site riding elephants, relax in ... Falls, Datanla Falls, Prenn Falls, Pongour Falls, Dambri Falls, Bobla Falls, Liliang Falls, Voi (Elephant) Falls, Pong Giang Falls, Golf Hills, Lang Bian Mountain,etc Places of historical and cultural ... nature Return to the harbor, tourists will go by car to visit Datanla Waterfall (Boat rental and elephant rental not included) - Da Lat - Pongouh Waterfall – spirit of The Highlands Destinations:...
... definitions of culture -5- provide culture with different values In this paper the author would like to emphasize the influence of cultural factors on the teaching and learning of speaking skills in classrooms, ... of the world around them.” (Kramsch, 1993:1) 1.1.2 The role of culture in language teaching To emphasize the importance of culture in learning and teaching foreign languages Eli Hinkel (1999) has ... and which for many is particularly conductive for learning” This means that there is a lot of emphasis put on the importance of speaking skills It is only when speaking skills are mastered that...