interactions of trypanosoma cruzi and triatomines
Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
... (Eur J Biochem 270) yield of 1.7 mg from a 50-mL culture of recombinant bacteria Preparation and purification of T cruzi gGAPDH T cruzi gGAPDH was expressed in E coli and purified following the ... with a standard deviation of ± 4% T brucei T cruzi 2000 2000 350 800 65 2000 150 900 200 700 HOP Inhibition and site-directed mutagenesis of T brucei gGAPDH In the absence of a 3D structure of a ... 5, and were in the range of the Km values for 1,3-BPGA and even up to three times lower for compound Selection of T brucei gGAPDH residues to be mutated and measurement of kinetic parameters of...
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Interactions of Chemical Variations and Biocide Performance at Paper Machines potx
... errors in demanding biocide dosing 15 THE OBJECTIVE AND THE OUTLINE OF THE STUDY The objective of this work was to study the interactions of microbial activity, biocide usage and - creation and chemical ... the DLVO (Derjaguin and Landau, Verwey and Overbeek) theory (Derjaguin and Landau 1941; Verwey and Overbeek 1948) The theory explains how particles in aqueous system interact and how different ... of the system and further the flocculation and coagulation Figure illustrates how different forces attract and repulse the particles, what is the effect of salt concentration, and what kind of...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... The reactivity of sera from chagasic patients against different fragments of cruzipain, the major cystein proteinase of Trypanosoma cruzi, suggests the presence of defined antigenic and catalytic ... laser desorption ⁄ ionization time of flight mass spectrometry in positive and negative ion modes Analysis of proteins of high molecular mass, and of cyclic and acyclic oligosaccharides Rapid ... Effects of proteinase inhibitors on growth and differentiation of Trypanosoma cruzi FEMS Microbiol Lett 124, 81–86 Bontempi E, Martinez J & Cazzulo JJ (1989) Subcellular localization of a cystein...
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Design for aesthetics: interactions of design variables and aesthetic properties pdf
... reference (frontal, profile, median) comparative size and spacing of features • degree of symmetry of arrangements of objects about centre of mass, major axes and planes of references of the whole product ... dimension of one object (or feature) and that of another A number of standard good proportion exist (e.g the golden section) Areal and volumetric refers to similar relations in the areas and volumes of ... coverage and variations of • complementary and opponent colours • different luminance intensity, hue, or saturation degree of asymmetry about centre of mass, major axes, and planes of reference...
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interactions of yeasts, moulds, and antifungal agents how to detect resistance
... including candidal esophagitis and hepatosplenic candidiasis; for severe candidiasis including candidemia, endophthalmitis, and osteomyelitis; and for treatment of aspergillosis, cryptococcosis, and ... drugs for treatment of chromoblastomycosis 1.2.4 Spectrum of Activity The spectrum of activity of 5-FC includes most species of Candida (except for C krusei) and C neoformans and Saccharomyces ... is little use of miconazole outside of treatment of dermatophyte infections, cutaneous Candida infections, and tinea versicolor With the advent of new azoles and echinocandins that offer more broad-spectrum...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
... properties of EGFP in CHO cells 64 Fig 4.2 Image and ACFs of PMT-EGFP expressed in CHO cells 67 Fig 4.3 Image and ACFs of EGFR-EGFP expressed in CHO cells 69 Fig 4.4 Images and ACFs of EYFP, mRFP and ... one-photon excitation, and it avoids the difficulty of the alignment of two laser lines or the cost of using an expensive pulsed laser Hwang and Wohland developed the theory of SW-FCCS and successfully ... 48 3.1.1 Construction of EGFR-FP fusions 48 3.1.2 Construction of ErbB2-EGFP and ErbB2-mRFP 51 3.1.3 Construction of mRFP-EGFR and mRFP-EGFR-EGFP 52 3.1.4 Construction of PMT-FPs 54 3.1.5 Summary...
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In vivo aspects of potential stereospecific drug interactions of oral warfarin and rutin in rats
... for the constant support and encouraging ii words of Assoc Prof Go Mei Lin, Assoc Prof Paul Ho, Assoc Prof Chan Sui Yung, Assoc Prof Lim Lee Yong, Assoc Prof Chan Lai Wah and Dr Shanthi I want to ... separation of warfarin enantiomers by HPLC 21 1.5 Chemical structures of flavonols, quercetin and rutin 28 3.1 Time courses of total serum concentrations (mean ± S.D.) of S- and R- enantiomers of warfarin ... metabolites of warfarin not appear to contribute to the activity of the drug But metabolite estimation helps in understanding the source of variation and mechanism of interaction of drugs with...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... Fernandes JF (1967) Differentiation of Trypanosoma cruzi in culture J Protozool 14, 447–451 27 Hariharan S, Ajioka J & Swindle J (1993) Stable transformation of Trypanosoma cruzi: inactivation of ... Experimental procedures Materials and reagents Fig Sensitivity to a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypanosoma cruzi Permeable parasites ... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and...
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Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
... granules and P-bodies of yeast, animal cells and T brucei (reviewed in [15–21]) Trypanosoma cruzi DHH1 is present in polysome-independent complexes and is located diffusely in the cytoplasm and in ... developmental stages of Trypanosoma brucei are extracellular, in both mammalian host blood and the tsetse fly intestine [1] Trypanosoma cruzi exists in its amastigote form in the cytoplasm of the mammal ... several aspects of mRNA metabolism and localize to distinct foci in the cytoplasm [26] We compared the replicative, nutritionally stressed, differentiating and infective forms of T cruzi, and showed...
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Interactions of Bioactive Plant Metabolites - Synergism, Antagonism, and Additivity
... portions of this chapter to refer to both approved drugs and bioactive plant compounds The binding of a drug to a protein may affect the function of that protein through a number of 10 Interactions of ... replicates and divided by average responses of in-tray control wells – and (2) taking the log of a function of the scaled data Each of these steps can have detrimental effects on the precision of parameter ... degree of receptor–receptor interactions, and the effects of drugs on the plasma membrane, particularly on the formation and composition of lipid rafts In the interest of brevity, other mechanisms...
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Tài liệu The Acquisition of Drugs and Biologics for Chemical and Biological Warfare Defense - Department of Defense Interactions with the Food and Drug Administration doc
... have been reordered by the events of September 11, 2001; officials in the Department of Health and Human Services and in the Department of Homeland Security; and the interested public This research ... reviewers of the draft report, Richard A Merrill of the University of Virginia School of Law and Bernie Rostker of RAND, also contributed to a stronger final report We are responsible, of course, ... Drug Application ORA Office of Regulatory Affairs ORO Office of Regional Operations OSD Office of the Secretary of Defense PB pyridostigmine bromide PEO Program Executive Officer PHSA Public Health...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... vant Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, ... governing ureaprotein interactions: m, mDHi and mDSi By using Eqns (57), at different urea concentrations, and the values of the DHmÂ, DSmÂ, Tm and DCp obtained by the approach of Pace and Laurents ... determination of the thermodynamic parameters governing rst, the HPr unfolding and, second, the ureaprotein interactions The combination of denaturant and heatinduced denaturation experiments gave proof...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... assay and expressed as percentage of the theoretical maximum The activity was monitored over time and the rate of latency transition expressed as the functional half-life, t½ The averages and standard ... assay and expressed as percentage of the theoretical maximum The activity was monitored over time and the rate of latency transition expressed as the functional half-life, t½ The averages and standard...
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Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf
... serotypes M1 (AP1 and 90–226), M5 and M6 (JRS4) were analysed for binding of 125I-labelled DS Isogenic mutants of AP1 (BM27.6, MC25, BMJ71), of 90–226 (90–226 emm1::Km), of M5 (DM5) and of JRS4 (JRS145), ... Epithelial cells and fibroblasts were labelled with [35S]sulfate, washed and incubated with chondroitinase ABC and HS lyase Triton extracts of untreated and enzymatic treated cells were prepared and analysed ... binding of eukaryotic proteins to various GAGs depends on electrostatic interactions between the negatively charged sulfate groups of the GAG chains and positively charged regions of the ligand Typically,...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... description of the elementary steps of enzyme–target binding and catalysis and then uses the rapidequilibrium approximation for protein–protein interactions for a systematic simplification of the model ... phosphorylated states and pathways of phosphorylation and dephosphorylation It will be an aim of this study to elucidate the consequences of processing order for the regulatory properties of the target ... Order of phosphate processing Sequential phosphorylation and dephosphorylation (first panel), random phosphorylation and dephosphorylation (second panel), mixed scheme with random phosphorylation and...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... N-terminus of HIPPI containing Myosin-like domain (HiN), and that expressing GFP-tagged pDED of HIPPI (HiD) The upper bands of the upper panel correspond to procaspase-8 (57 kDa), and the lower bands ... concentrations of NaCl, the fluor3888 escence intensities of GST–HIPPI increased, indicating a lesser extent of interactions of GST–HIPPI with AA AGACATG This result indicated that the interaction of GST–HIPPI ... 1/[DNA]µM III Fig In vitro binding assay of putative HIPPI-binding motif AAAGACATG and its mutant (A) EMSA of binding of the end-labeled bp motif AAAGACATG and two of its mutants with purified GST–HIPPI...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... to the C-termini of other members of the secretion family, such as PilQ of Myxococcus xanthus [28], ExeD of Aeromonas salmonicida [29], PilQ of P aeruginosa [25,30], and PilQ of N gonorrhoeae ... Analyses of novel Thermus competence proteins J Rumszauer et al N-terminal domain of PilQ, and the colocalization of PilQ with PilW in inner and outer membranes and the results from the pilW and pilQ ... PilA4 and PilQ are individually essential for transformation and piliation [7,8] In contrast to PilQ and PilA4, an individual role of PilM, PilN, PilO and PilW in natural transformation and piliation...
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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx
... the addition of complexes were performed For the analysis of interactions between proteoglycans and collagens, the sensograms were corrected by a modification of the method of Roden and Myszka [20] ... interaction of wild-type decorin and decorin mutants with type I and type VI collagen and, for the first time, we analysed the formation of triple complexes consisting of type VI collagen, decorin and ... involving dimerization of decorin and binding of both collagens to the inner surface of decorin (B) Interaction of type I collagen with the inner surface of the decorin core protein and type VI collagen...
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Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family ppt
... above The O2 and O3 of 4PEH correspond to O3 and O4 of the R5P structure (Fig 1) Accordingly, O3 of 4PEH interacts with His102¢ and a water molecule, whereas O2 hydrogen bonds to Asp10 and to the ... 4PEH and R5P, the protein was mixed with ligand (dissolved in water) to give a final ligand concentration of 20 mm For the TcRpiB-C69A ⁄ All6P structure, the concentration of ligand was 10 mm, and ... was added at a concentration of 30 mm The same preparation of TcRpiB had a kcat of 28 s)1 and a Km of mm when R5P was the substrate, measured directly using the A290 of Ru5P [14] The Lineweaver–Burk...
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ECOLOGY OF COLD SEEP SEDIMENTS: INTERACTIONS OF FAUNA WITH FLOW, CHEMISTRY AND MICROBES potx
... addresses the communities of organisms that inhabit cold seep sediments, focusing on soft-bodied, mid-size organisms (e.g., macrofauna and meiofauna) and on the nature of their interaction with ... environments often support surprisingly high densities of macrofauna Estimates of density vary with the mesh size employed but values of >10,000 ind m–2 are common and local patches of >40,000 ... fractures and sand layers or via mud diapirs Cold seeps are commonly found along fractures at the crests of anticlines, on the faces of fault and slump scarps where bedding planes outcrop and along...
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