integrated proteomics technologies and in vivo validation of molecular targets
Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... lung, and kidney were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats...
- 7
- 391
- 0
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...
- 9
- 422
- 0
Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA targets ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation...
- 10
- 463
- 0
Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and ... 3a) and because these cytokines have been shown to synergistically induce the expression of iNOS [34] and IDO [35] in fibroblasts The expression of iNOS, IDO, PD-L1, and COX-2, molecules involved ... cytokines The serum of mice was pooled per group and analyzed for the T-cell cytokines IL-2, IL-5, IL-6, IL-10, and IFN-g The injection of anti-CD3 antibody caused a profound increase in cytokine...
- 11
- 464
- 0
Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx
... effects of sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating ... the in vivo studies of N569 and A663 strains were independently performed and carried out under different conditions At this point, a direct comparison of the pathogenecity of these strains is ... meningoencephalitis in calves reported in 1962 in Australia [10] The A663 BoHV-5 strain was isolated from a case Page of of non purulent encephalitis in an outbreak among calves in 1982 in Argentina [19] N569 and...
- 8
- 366
- 0
Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx
... study, and informed consent was obtained from the patients' next of kin Hygrometric measurements AH and relative humidity were measured during the inspiratory phase in the in vitro and in vivo ... 12 different settings Combinations of two tidal volumes (0.5 and l), two peak inspiratory flow (0.5 and l/s) and three levels of minute controlled ventilation (5, 10 and 15 l/min), with the ventilator ... conditioning remains debatable [1,2] For example, Williams and coworkers [20] suggested that heating and humidifying the inspired gas to the natural targets of core temperature (i.e 37°C) and AH of...
- 8
- 360
- 0
Báo cáo khoa học: " In vivo validation of the adequacy calculator for continuous renal replacement therapies" pps
... achieved In this state of absence of information and of wide ignorance of the field, the calculator might have the merit of placing the issue of treatment dose among the priorities of critical ... Creatinine levels after 24 h from RRT start (mg dl-1) 2.4 ± 1.25 Creatinine levels at RRT withold 1.8 ± 0.6 No of patients receiving heparin < 500 U h-1 12 No of patients receiving heparin > ... settings depending on the preference of the prescribing physician, but a final spKt/V of 1.4 had to be prescribed by means of the Adequacy Calculator The plasma filtration fraction, in the case of...
- 8
- 335
- 0
In vitro and in vivo study of ABT 869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors insight into molecular mechanism and biologic characterization
... Phosphoinositide 3-kinase Promyelocytic Leukemia Zinc Finger protein Phosphatase of Regenerating Liver Retinoblastoma Protein Red Fluorescence Protein RNA interference Receptor Tyrosine Kinase Standard ... down-regulated cyclins D and E by 16h and induced the expression of p21waf1/Cip progressively The increasing expression of cyclin E in MV4-11 cells at 4h, in MOLM-14 cells at 1h and cyclin D in MOLM-14 ... Shock Protein 90 Indirubin Derivative Interferon gamma-inducible gene 16 Immunohistochemistry Intraperitoneally Internal Tandem Duplication c-Jun N-terminal Kinase Kinase Insert Domain Receptor...
- 121
- 368
- 0
In vitro and in vivo assessments of PCL TCP composites for bone tissue engineering
... engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, maintenance and substitution of damaged tissues and organs using principles of ... for signs of sustained delivery when immersed in PBS solution Heparin was chosen to improve binding and regulate release of BMP-2 here and concurrently act as a framework for binding of endogenous ... Simic and Vukicevic, 2005) and kidney formation (Simic and Vukicevic, 2005) There exists a heparin binding site in N-terminal region of mature BMP-2 polypeptide In pioneering work by Ruppert in...
- 94
- 546
- 0
In vitro and in vivo characterisation of recombinant lactobacilli expressing house dust mite allergen
... IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI EXPRESSING HOUSE DUST MITE ALLERGEN LIEW LEE MEI 2009 IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI ... leukotrienes and basic proteins (cationic proteins, eosinophil peroxidase, major basic protein and eosinophil-derived neurotoxin), and they might be an important source of interleukin-3 (IL-3), ... of cytokines in splenic cultures Figure 4.5 Oral feeding of recombinant Lactobacillus rhamnosus GG 108 induced the Blo t 5-specific IgG2c production in mice Figure 4.6 Oral feeding of recombinant...
- 193
- 613
- 0
In vitro and in vivo evaluation of customized polycaprolactone tricalcium phosphate scaffolds for bone tissue engineering
... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... project and allowing the author to join his team, for his expertise, kindness, and most of all, his patience His enthusiasm in research and continuous support have truly been a source of inspiration ... porosity of about 85% was targeted for 1.3.2 Part 2: Optimization of native and customized scaffolds in vitro and their effects in initial bone healing (in Chapter 4) In the second part of the...
- 143
- 472
- 0
In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude ... (anti-microtubular) agents include the naturally occurring vinca alkaloids (e.g vincristine and vinblastine) and their semi-synthetic analogues (e.g vinorelbine) and the taxanes (e.g paclitaxel and docetaxol) ... protein binding after IV administration (Extra et al., 1993) Docetaxel is mainly bound to alpha acid glycoprotein, lipoproteins, and albumin Among them, alpha acid glycoprotein is the main determinant...
- 129
- 286
- 0
In vitro and in vivo investigation of nanoparticles of a novel copolymer for substained and controlled delivery of docetaxel
... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... causing the clearance of drugs once they are present in physiological system is the high probability of binding to endogenous proteins in the circulatory system The high-binding affinity of most...
- 163
- 932
- 0
In vitro and in vivo study of vitamin e TPGs coated immunoliposomes for sustained and targeted delivery of docetaxel
... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... and reversibly to the β- subunit of tubulin in the microtubules rather than to tubulin dimers The binding site to tubulin differs from the one of vinca-alkaloids and podophyllotoxins While vincas...
- 121
- 326
- 0
Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
... be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of DCs which should be superior to in vitro loading of DCs with tumor ... mobilization of DCs using Flt3 ligand, local injection of chemokines such as macrophage inflammatory protein (MIP)-3β, use of DNA vaccines containing bacterial CpG motifs and the use of topical compounds ... both in vitro and in vivo experiments (Steinman and Pope, 2002) As with other APCs, DCs play an important role in activating both the innate and adaptive components of the immune system via interaction...
- 130
- 331
- 0
Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... Akt and ERK insulin signaling pathways and inhibits distal effects of insulin on glycogen and DNA synthesis [6–9], and microinjection of Grb14 into Xenopus laevis oocytes inhibits insulin-induced ... of insulin administered in vivo to increase the level of RCAM-lysozyme in cell fractions, and then assayed the fractions of insulin-injected rats for Grb14, IR and phosphorylated IR content and ... [13], and tankyrase [15] The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phosphorylation of Grb14 and its inhibitory effect on IR kinase and insulin signaling...
- 15
- 497
- 0
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used ... growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose (m) or in...
- 9
- 548
- 0
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... role, such as in DNA replication, in living cells In contrast, mutants of AtRPA70b were viable but showed high sensitivity to UV and MMS, suggesting involvement of AtRPA70b in the repair of damaged ... region, and a third, DBD-C, in the C-terminal region DBD-A and DBD-B are the most important regions for binding of ssDNA, whereas DBD-C has only weak ssDNA-binding activity At the N-terminus of RPA70kDa...
- 12
- 588
- 0
Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in ... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... cared and handled in conformence with the Guiding Principles for Research Involving Animals and Humans, approved by the Council of the American Physiological Soceity The School of Medicine Research...
- 9
- 508
- 0
Báo cáo khoa học: A study of microRNAs in silico and in vivo: diagnostic and therapeutic applications in cancer pot
... growth of breast cancer cells in vitro and in xenografts in mice [48] miRNAs as biomarkers in cancer Their fundamental role in development and differentiation, and their pervasive corruption in lineagedependent ... in CLL, promoting the survival of leukemia cells [31] Indeed, there is a reciprocal relationship between the expression of miR-15a and miR-16-1 and of Bcl-2, and the heterologous production of ... region of 30 kb in which miR-15a and miR-16-1 reside, which are lost in 70% of patients with CLL [29] Similarly, the loss of chromosomal region 13q14, including miR-15a and miR-16-1, occurs in...
- 8
- 432
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25