... template in the presence of synthetic oligonucleotidespairs.Primer 1: 5Â-AAGAAGAAGCATATGTCACCGTGCCCGACCAGCTG-3ÂPrimer 2: 5Â-AAGAAGAAGCATATGACCGCCACGGAAACAGTCCGG-3ÂThe primers introduced NdeI ... 6A, curves 2–16). The calculated radius of gyra-tion (Rg) and molecular mass (MM) increased atincreasing protein concentration (Table S6), indicatingthe presence of multiple species in solution. ... medium (to measure the cells vitality) or medium containingampicillin (to count cells containing the plasmid) in the absence or presence of isopropyl thio-b-d-galacto-side (IPTG) (to establish...
... 5Â-TAATACGACTCACTATAGGGA*5Â-AACACACCATTCCTGCCAAC-3ÂCCACCACAGGTCGCGCC-3ÂAcnB 5Â-TAATACGACTCACTATAGGGA*5Â-CTCACACGCTGCTGATGTTC-3ÂCGTGGTTACGCACTTCACC-3ÂPrpD 5Â-TAATACGACTCACTATAGGGA*5Â-AACATCGGCGCGATGATCC-3ÂTCGCTGCTTCAACTGCCG-3PrpE ... 5Â-TAATACGACTCACTATAGGGA*5Â-AACATCGGCGCGATGATCC-3ÂTCGCTGCTTCAACTGCCG-3PrpE 5Â-TAATACGACTCACTATAGGGA*5Â-ACCGGAGCAGTTCTGGGC-3ÂGATTCCAGCCACGCCACC-3Âể FEBS 2002 Isomerization of methylcitrate to 2-methylisocitrate (Eur. J. Biochem. 269) 6187 catalysed ... 5Â-CGGGATCCTCAGCTCAAATCAACAACATCCGC-3Â)andPstI(5Â-AACTGCAGTTAAATGACGTACAGGTCGAGATAC-3Â), respectively. After restriction of the PCR productwith both enzymes, the product was cloned into thepreviously...
... remains insignificant. We also experimented with other measures of individual in- come uncertainty: the income quintile share ratio (income of the 20% richest to income of 20% poorest), the percentage ... profile becomes lower. As a result, higher θ means that theprecautionary motive to accumulate savings is diminished, which leads to a decline in the stock of capital. An increase of the replacement ... 2007).Our aim is to contribute to the above literature by proposing a life-cycle model withindividual income uncertainty that can be used to assess how various macroeconomicfactors affect the equilibrium...
... Reference Choice is Car Owner and Motorcycle, Car Driver or Car Passenger)(contd.)No Car One or More CarsOn Foot Bus or Motorcycle, On Foot Bus oror Bicycle Train Car Driver, or Bicycle TrainCar ... Reference Choice is Car Owner and Motorcycle, Car Driver or Car Passenger)No Car One or More CarsOn Foot Bus or Motorcycle, On Foot Bus oror Bicycle Train Car Driver, or Bicycle TrainCar PassengerIndividual-specific ... TRANSPORTATION OFFICE, 2005. Road User Monitoring Report 2004,Dublin: Dublin Transportation Office.DUBLIN TRANSPORTATION OFFICE, 2006a. DTO Cycling Policy, Dublin: DublinTransportation Office.DUBLIN...
... allowed studyingthe cellular structureof AC in vivo [28-30]. Although thestaining technique used in this study is not clinical appli-cable, our current study on the investigation of clinicalviable ... Fibre optic laser confocal microscopyuses an optic fibre to perform the function of a pinhole in a conventional confocal microscope to obtain images of bulk biological tissues [27]. The optic fibre ... imaging technique as a tool for examining the micro-scopic degeneration of the collagen network and early OA.A schematic structureof the collagen network in AC shows that the interwoven collagen...
... London CouncilInstitution of Civil EngineersInstitution of Structural EngineersNational Association of Scaffolding ContractorsStainless Steel Fabricators’ Association of Great BritainSuspended ... to an accuracy of0 .02mm.4.3 Load measuring device, being a pressure gauge, proving ring, load cell or other suitable device, capable of measuring to an accuracy of5 %.4.4 Structural fixings, ... fully cured in accordance with the supplier’s recommendations.6.4 Cast -in fixingsFor cast -in fixings, the fixing shall be installed in accordance with the supplier’s recommended procedure...
... geographic x- and y- coordinates of eachmunicipality was obtained from The National Land Sur-vey of Sweden [11,12].An official grouping of Swedish municipalities intonine groups according to number ... AMI, incidence and mortality/1000 Statin utilisation DDD/TID * * Change of cut off level of Cardiac troponin T, troponin I or creatine kinase (CK-MB) for AMI. Figure 1 Incidence and ... Medicine and HealthSciences, Faculty of Health Sciences, Linköping University, S-581 83Linköping, Sweden.2Vikbolandet Health Care Centre, Department of PrimaryHealth Care, County Council of...
... I declare that I have NOT financialand non-financial competing interests in relation to thismanuscript.3. Milton Rossman: I declare that I have NOT financialand non-financial competing interests ... NOT financial andnon-financial competing interests in relation to thismanuscript.6. Ella van den Berg-Loonen: I declare that I have NOTfinancial and non-financial competing interests in rela-tion ... for citation purposes)BackgroundDue to its unique chemical-physical properties, beryllium(Be) compounds continue to be used in aerospace, ceram-ics, defence, electronics and telecommunication...
... days of treatment per week(s), respect of the exact time schedule of obtaining the medications,etc] and used different cutoff point of adherence (from80% to 100% of dosage) in order to dichotomize ... allowing for higher income patients to adjust according to relative social status, possibly beinginfluenced by other SES factors such as education andoccupational status [13].The existence of ... to benoted that having a medical insurance and easy access to primary care, receiving treatment by the same medicalproviders each time, receiving counseling by specialists,and not having to...
... Galiana, pers comm 1995)We can think of Drosophila population structure as consisting of an array of localbreeding populations with high extinction and recolonization rates. ... wing length of D buzzatii and the number of D simulans might also indicate the occurrence of interspecific competition,but some care must be taken with such an interpretation ... wing length from each control and supplemented half fruit was calculatedby weighting the mean of each collection by the number of males of each species in that collection.Allozyme...
... quickly, creating an initial classification of semantic formulas and ascertaining the structures of speech acts under consideration.” However, a major difficulty in using a DCT for research of ... noticeable in the use of indirect speech acts. According to Yule (1996), indirect speech acts are generally associated with greater politeness in English than direct speech acts. Hence, interlocutors ... According to Grice (1975), we are able to converse with one another because we recognize common goals in 25 maxims provide a basis for the hearer to construct a sequence of inferences which...
... 24DirectorDirectorDirector AssistantDirector AssistantVice DirectorVice DirectorVice DirectorVice DirectorProduction(96 prs.)Production(96 prs.)Planning(4 prs.)Planning(4 prs.)Purchasing(5 ... been increased together with their income per capita also increases, especially in large cities such as Hanoi and Ho Chi Minh Cities. In addition, the number of personal computers has been increased ... vision of its business. On the basis of this, it can construct an effective communication program to its target audiences, which are now expected to cover the whole electric and electronic range....
... extension of the fingers, so it is horrible to suck chopsticks before picking up food. However, dipping chopsticks into soupbowl before eating is acceptable as this act will prevent rice from sticking ... bustle.3.2.2. Alcohol/wineBoth alcohol and wine are excited drinks in which alcohol (in full ethylalcohol) is a colourless volatile inflammable liquid forming the intoxicatingelement in wine, beer, ... occupier, its meaning ischanged according to the way people call it. At the beginning or ending of ameal, the phrase “food tray” is called so much that is stands for meal in the way of calling...