in vivo evaluation of gene transfer into mesenchymal cells in view of cartilage repair
Molecular basis of agrobacterium mediated gene transfer into mammalian cells 5
... transformation In this study, ChvG was found to be involved in DNA transfer into mammalian cells and the regulation of acid-inducible genes, including aopB and katA, residing on the circular and linear ... occurrence of horizontal gene transfer among prokaryotes is well established, the gene transfer between bacteria and eukaryotic cells is less well elucidated The finding that non-specific DNA transfer ... vir genes, virB and virE Thus, ChvG might be a global sensor protein that can directly or indirectly sense extracellular acidity since it can regulate unlinked acid-inducible genes encoding different...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4
... 1987; Parkinson and Kofoid, 1992; Parkinson, 1993) 4.1.2.1 The kinase core module The kinase core is about 350 amino acids in length and is responsible for binding ATP/ADP and directing kinase transphosphorylation ... the ATP-binding domain, P4 (Hess et al., 1988) Other systems derived from typical HPK use His-containing phosphotransfer domains (HPt), distinct from the His-containing dimerization domains, which ... present in the promoters of most of the virulence genes (Powell et al., 1990) This binding will result in the induction of the vir gene expression Non-phosphorylatable mutant VirA and VirG protein...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 3
... expression of vir genes is minimal The data showed that the gene transfer efficiency of these A tumefaciens cells was similar to that of the induced cells, indicating that vir gene induction is ... chromosomal gene in DNA transfer into mammalian cells A tumefaciens cells with mutations in certain chromosomal genes are defective in the transformation of plant cells Thus, several strains with ... this inter-kingdom genetic information exchange 3.2.5 Internalization of A tumefaciens into mammalian cells 3.2.5.1 General characteristics of internalization Unlike plant cells, mammalian cells...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 2
... 0.8 µm intervals along the apical and basal axies of the cells 2.8.7 Observation of actins in mammalian cells Rhodamine phalloidin (Molecular Probes) was used to label actin of mammalian cells ... About h before incubation, the cells were washed once with PBS and incubated in fresh medium without antibiotics For the study of the staining pattern of rhodamine phalloidin, mammalian cells were ... analysis of proteins unless otherwise specified 2.6.3 Staining of SDS-PAGE separated proteins with standard Coomassie blue SDS-PAGE separated proteins were stained according to the instructions of...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 1
... integration of the T-DNA into the plant genome is still unclear Rossi (1996) suggested that, instead of contributing to the efficiency of integration, VirE2 might be involved in maintaining the integrity ... between the bacterial ros gene and its eukaryotic counterparts It is interesting to note that the zinc finger in Ros is akin to the zinc finger domain in animal cells rather than in plant cell, which ... vir gene induction, T-DNA processing, T-DNA transfer and nuclear targeting, T-DNA integration into the plant genome and transferred gene expression Briefly, the TDNA transfer process is initialed...
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Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells
... Complement inactivation is a major obstacle affecting the gene transfer efficiency of BV in vitro in serum-containing medium conditions, and in vivo during systemic administration A Fabrication of Bio-nanoparticles ... non-invasively assess BV delivery to the target cells or tissues of interest using experimentally and clinically relevant imaging methodologies At present, reports of in vivo imaging of BV in ... thus minimizing the toxic risk caused by high concentrations of gene delivery vectors In addition to improving gene delivery into cultured cells, this method can be used to control in vivo gene...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5. Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at the equivalent...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
... der: The influence of contact conditions on resonance frequencies of a hip stemfemur system: a finite element analysis In Proceedings of the International seminar Prediction and evaluation of total ... detection of the insertion endpoint Figure curing FRF graphs of a typical stem-femur system during cement FRF graphs of a typical stem-femur system during cement curing The evolution of the FRF ... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing...
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báo cáo hóa học:" An in vivo evaluation of bone response to three implant surfaces using a rabbit intramedullary rod model" doc
... gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum HA fraction in the crystalline phase was 90% ... finite dissolution rate of crystalline hydroxyapatite provides a continuous source of calcium and inorganic phosphate [18] In our present study, as well as in those reported by others, bone often ... osteoconductive, in vivo the surface adsorption of proteins (such as bone morphogenetic proteins) may render the surface osteoinductive [38,39] In addition, osteoblasts may attach and release active osteoinductive...
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Báo cáo y học: "In-vivo evaluation of simultaneous administration of incompatible drugs in a central venous catheter with a decreased port to port distance" pps
... length of cm, for the in vivo administration of incompatible solutions, phenytoin and TPN, using a swine model, did not lead to precipitates large enough to cause adverse clinical effects in our ... extravasation of fluids or drugs References Recker DH, Metzler DJ: Use of the multilumen catheter Crit Care Nurse 1984, 4:16 Jaimovich DG, Rose WW: In vivo evaluation of simultaneous administration of incompatible ... catheters in the pediatric population, and especially in the pediatric intensive care setting, are commonly used for the administration of intravenous fluids, drugs, chemotherapeutic agents, inotropic...
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Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx
... the offset was maintained, with no significant variations We used this offset (in the order of 0.1–0.2°C) to correct the measurements obtained during the study In each condition, the average of ... is increased [16,30], causing an increase in carbon dioxide levels, especially during low tidal volume ventilation [31] HMEs also cause an increase the inspiratory work of breathing, with an increase ... 30 mgH2O/l) when using HMEs in patients with high minute ventilation (between 10.5 and 16.5 l/min) [19,23,24] In the present study we found that increasing or decreasing the minute ventilation...
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In vitro and in vivo evaluation of customized polycaprolactone tricalcium phosphate scaffolds for bone tissue engineering
... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... into QS (QuickSlice), slicing of the CAD model into horizontal layers and conversion into SLC format, creation of a deposition path for each layer and conversion into SML format for downloading ... dissolve in acids Their solubility, defined as the amount of dissolved solute contained in a saturated solution when particles of solute are continually passing into solution (dissolving) while...
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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... discussed in Chapter In Chapter 5, in vitro cellular study of transferrin conjugated LPNPs is performed using MCF7 human breast adenocarcinoma cells In Chapter 6, in vivo pharmacokinetics, complete ... microtubules, an essential part of the cytoskeleton of eukaryotic cells The vinca alkaloids prevent the protein from polymerizing into microtubules by binding specifically to β-tubulin In contrast, the taxanes ... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude...
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Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells docx
... equivalent to 35% of that of the control Virus-based methods are the most successful of effecting gene transfer to neuronal cells [16,28] The increasing use of viral vectors to transfer DNA into neurons ... Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection Cell Biol Int 2005, 29:976-979 20 Lei L, Liu ZH, Ohta S, Yamada G: [In vivo transduction of EGFP into ... Probes Inc., Eugene, OR) and were diluted 1:1000 in PBS containing 0.2% Triton X100 Cells were stained using μg/ml of DAPI (Sigma) in PBS and then mounted on cover slips using Fluoromount-G Lin...
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Báo cáo khoa hoc:" Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems" pptx
... fixation and staining of actin rings (a-c) and TRACP (d) Baseline control is shown in a and d, and adenovirus-infected cells h post infection are shown in b A typical view of the cells incubated h ... delivery of the Page of (page number not for citation purposes) Journal of Negative Results in BioMedicine 2005, 4:5 Figure Number 2of actin rings in osteoclast cultures Number of actin rings in osteoclast ... Visualization of actin rings and TRACP-positive cells in osteoclast cultures Visualization of actin rings and TRACP-positive cells in osteoclast cultures Osteoclasts were differentiated in the presence of...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... Correspondingly, in vivo implantation of BMP-4 into abdominal muscles of rats led to ectopic cartilage and bone formation when delivered as recombinant protein [38] or via genetically modified cells ... sustained regeneration of hyaline cartilage in vivo [7] Inadequate delivery of the soluble factors necessary to drive the chondrogenic differentiation of the transplanted cells in vivo is a major ... capable of driving chondrogenesis in culture [19,20] In the present study, using adenoviralmediated gene transfer our aim was to compare the effects of BMP-4 and BMP-2 expression on chondrogenesis of...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... with other proteins such as the PSI core Incorporation of tagged PSI-G into PSI Whilst it is evident that both C-terminally and looptagged PSI-G can insert into the thylakoid in vivo (Figs and...
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Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc
... previously shown that the DHS at 185 + 10 kb in intron of the CFTR gene augments intestinal expression of the gene in vivo, both in human colon carcinoma cells and in transgenic mice [15] It is probable ... the DHS in intron 18 is more prominent than those in introns 16 and 17 (Fig 5A) in chromatin from Capan1 cells The DHS in introns 1, 10a,b (very weak) and 20 were also evident in Capan1 cells (data ... showed an increase in intensity following exposure to activators of CFTR transcription were DHS10a, b, following forskolin activation in Caco2 cells (Fig 7A) and DHS 18 in Capan1 cells following mitomycin...
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Báo cáo khoa học: Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes Evidence for the participation of other residues doc
... 23 or 53 but not in peptides containing tyrosine 20 alone According to these results, tyrosines 23 and 53 could be involved in the dimerizations whereas no sign of tyrosine 20 involvement was ... obtained by nonlinear least-squares fitting to the absorbance data The numbering of the amino acid residues in each lysozyme protein follows that of the Protein Data Bank (PDB) of the Brookhaven ... of fragmentation at the peptidic bond between Asn103 and Gly104 for both proteins In addition, N-terminal sequencing of the smaller moiety indicated that Gly residue is intact It is interesting...
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Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... promoter silencing does not happen in particular cases, including our study, remains unknown However, in vitro [60] and in vivo [61] studies have shown that AAV vectors induce a change in gene expression ... following issues through future long-term in vivo studies: (a) evaluation of silencing of the promoter, and the resultant level of coexpression of SUMF1 and GALNS; and (b) confirmation of targeting ... with improvement in polyadenylation ⁄ transport and mRNA processing, which resulted in a sixfold to 50-fold increase in the indicator (CAT) protein [40] The presence of introns in expression plasmids...
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