identification of smis of rpa 6a emsa analysis of compounds identified from the high throughput screen performed for rpa 25 nm rpa was incubated with 100 µm of each compound for five minutes to which 25 nm sjc 1 5 xba 34 nt dna was
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... A34 853 43.2 37.6 28.0 28 .5 25. 2 25. 2 45. 7 39.3 26.6 26.6 25. 1 25. 1 CAA24863 27.4 AAB2 614 8 28.0 CAA24863 27.0 26.9 26.6 25. 7 AAG16 755 NP_0 611 94 AAK62468 AAG 414 01 NP_060872 16 .0 19 .0 13 .8 14 .3 15 .0 ... lL of cDNA was mixed with lL of dNTPs (10 lM of each dNTP), 10 · Gene Taq Universal buffer, 0 .5 lL of Taq polymerase (5 UÆmL )1; Nippon Gene, Tokyo, Japan), lL of each gene specific primer (5 lM), ... at the 5 (GT) and 3¢ (AG) ends of each intron The four introns are 15 0, 242, 444 and 11 9 bp in length, respectively However, while the mammalian counterpart spanned > 5. 5 kb, the carp IL -10 was...
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Báo cáo khoa học: "Automatic Identification of Word Translations from Unrelated English and German Corpora" pot
... the German corpus, we used 13 5 million words of the newspaper Frankfurter Allgemeine Zeitung (19 93 to 19 96), and as the English corpus 16 3 million words of the Guardian (19 90 to 19 94) Since the ... started with a list of 10 0 German test words as proposed by Russell (19 70), which he used for an association experiment with German subjects By looking up the translations for each of these 10 0 words, ... frequency of B All co-occurrence vectors were transformed using this formula Thereafter, they were normalized in such a way that for each vector the sum of its entries adds up to one In the rest of the...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... 70 .5 )12 7 )11 5 .1 14 .1 P1 / w / w 11 7.2 15 .7 14 0.6 6 .5 ) 70.6 21. 1 ) 56 .7 13 .4 15 .6 62.6 ) 51 . 4 31. 7 ) 11 .8 71. 7 ) 15 0 .5 87.7 11 8.2 45. 3 ) 17 5. 3 99.4 12 3.4 22.0 )17 5. 5 ... 86.4 )11 4 .5 60.7 12 6.4 28 .1 127.4 34. 2 16 3.7 65. 8 )48 )12 3.0 19 .3 15 1.8 76.6 15 1 87.0 27.4 )12 3.6 87.8 )13 0 13 )93.4 10 .3 12 5. 9 30 .1 117 19 65. 4 11 .1 Arg18 Cys19 Molecule ... 9 11 , 16 19 , 25 27a 27±32b a b sheet regions; b Heavy 0.76 0 .55 0.39 0.78 0 .17 0 .11 0 .11 0.23 1. 35 1. 18 0.94 1. 35 0.23 0.20 0 .19 0.33 1. 03 0.68 0. 51 1.22 0. 31 0 .14 0 .12 ...
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báo cáo hóa học: "On the identification of sensory information from mixed nerves by using single-channel cuff electrodes" pot
... 3.8230 1. 4292 Rat 2 .19 37 3.66 01 1. 858 4 Rat 1. 959 2 2.2802 1. 6879 Rat 2. 059 2 3.0 752 2. 053 0 Rat 1. 51 4 0 2.2923 1. 1692 Mean ± Standard Deviation 2.0328 ± 0.3 411 3.0262 ± 0.73 05 1. 63 95 ± 0 .348 8 Stimuli ... parameters was analyzed first Therefore, different observation window lengths were studied [ 25, 50 , 75, 10 0, 12 5, 15 0, 200, and 300 ms], and for the best performing lengths, different overlaps [1/ 4, 1/ 2, ... first half of the signals was used to train the parameters of the classifiers; their performance was then assessed on the second half of the data The performance of the classifiers was measured...
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báo cáo hóa học:" Low RBM3 protein expression correlates with tumour progression and poor prognosis in malignant melanoma: An analysis of 215 cases from the Malmö Diet and Cancer Study" potx
... 4.39 (1. 65 -11 . 65) 1. 93(0. 51 - 7.26) 10 3( 21) 1. 70(0. 85- 3.40) IV-V 51 ( 21) 9.99(3.76-26 .55 ) 1. 02(0.24-4 .34) 51 ( 18) 3.04 (1. 49-6.22) Breslow Continuous 248(47) 1. 07 (1. 03 -1. 11) 248 (53 ) 1. 07 (1. 03 -1. 12) ... LMM, Other 19 5( 22) 1. 00 19 5( 30) 1. 00 1. 00 Nodular 53 (24) 5. 63(3 . 15 -10 .08) 53 (22) 3.86(2. 21- 6.74) 2.32 (1. 20-4.94) 213 (30) 35 (16 ) 1. 00 5. 80(3 .12 -10 .77) 1. 00 6.29(3.46 -11 . 45) 1. 00 3 .52 (1. 63-7. 61) Ulceration ... RR( 95% CI) 255 (53 ) 1. 09 (1. 04 -1. 13) 1. 07 (1. 02 -1. 12) 13 2 (18 ) 1. 00 2.60 (1. 47-4. 61) 1. 00 2.37 (1. 22-4 .57 ) 1. 00 n(events) Age Continuous Gender Female Male Clark level II 93 (5) 1. 00 1. 00 93 (13 ) III 10 3( 21) ...
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Báo cáo hóa học: " First identification of primary nanoparticles in the aggregation of HMF" potx
... Transmittance 797 2 9 25 10 22 13 95 3300 15 10 16 04 17 04 16 37 4000 350 0 3000 250 0 2000 15 00 -1 Wave Number (cm ) Figure 10 00 50 0 # * HMF 250 200 15 0 10 0 50 Chemical Shift (ppm) Figure -50 Figure ... Schematic illustration of the formation mechanism of carbonaceous spheres from fructose under hydrothermal condition -7- (a) (b) 10 0 nm 50 nm (c) (d) 10 µm 10 0 nm (e) (f) µm Figure 10 0 nm Figure Transmittance ... vibrations at 1, 604, 1, 51 0 and 1, 3 95 cm 1 are the characteristic band stretches of the five- member heteroaromatic ring with double bonds [ 15 ] The bands at 800 to 700 cm 1 may be assigned to strong...
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báo cáo hóa học:" First identification of primary nanoparticles in the aggregation of HMF" doc
... 797 2 9 25 10 22 3300 15 10 16 04 17 04 13 95 16 37 4000 350 0 3000 250 0 2000 15 00 10 00 50 0 -1 Wave Number (cm ) Figure TEM image of primary nanoparticles in a residual fructose solution after hydrothermal ... significantly from the conventional understanding in the open literature Page of 5 10 11 12 13 Acknowledgements The authors wish to acknowledge the financial support from the Department of Innovation, ... http://www.nanoscalereslett.com/content/7 /1/ 38 Page of (a) (b) 10 0 nm 50 nm (c) (d) 10 μm 10 0 nm (e) (f) μm 10 0 nm Figure SEM and TEM images of carbonaceous spheres, revealing details of grainy surfaces and interiors Carbon...
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báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs" docx
... i with 1 11 = {2, 6, 8, 8, 12 , 12 , 4, 10 , 10 , 16 , 16 } (2) Moment invariants can be derived both from a gray-level image and a binarized image We incorporate the invariants ψ ψ 11 of both the ... [http://www.springerlink.com/content/ m4 417 55 p245l 15 21/ ] Springer Hu MK: Visual pattern recognition by moment invariants IRE Transactions on Information Theory 19 62, 8(2) :17 9 -18 7 Flusser J: On the independence of rotation ... obtained a sketch of the color images, representing the objects of interest As a last milestone, the identified potential haustoria regions must be classified into true haustoria and false positive...
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Báo cáo y học: "Assessing the diagnostic accuracy of the identification of hyperkinetic disorders following the introduction of government guidelines in England" pdf
... 0.0 01) The three teams (B, M, and F) each contributed 19 8, 10 6, and 19 8 cases to the sample, with 11 , 10 , and 11 discordant cases respectively (Fisher's exact test, p = 58 ) As DMF was also one of ... disorders, which is consistent with previous community [11 ] and clinic [10 ] samples before or after the introduction of Government guidelines While the very high levels of agreement between the SDQ ... the paper for publication Berkshire Mental Health NHS Trust approved the design, and gave managerial support to data collection 10 11 12 13 14 15 16 Authors' contributions DF initiated the study,...
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Báo cáo y học: "Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors" pot
... 10 0 9 10 0 95 8 90 95 MS1 90 85 80 75 7 8 70 R e la ti v e a b u n d a n c e 85 80 75 70 65 60 55 1 6 50 2 45 40 1 35 R e l a ti v e a b u n d a n c e 65 30 60 20 1 9 25 2 4 4 3 9 4 15 10 55 50 ... the BiNGO tool After identification and merging of the two datasets by the Protein Center tool, a gene list of the 4 91 identified proteins was generated and submitted to the BiNGO tool This tool ... Plasma protease C1 inhibitor† SERPING1 5, 7 Neuroserpin SERPINI1 14 Stratifin† SFN 15 Thrombospondin -1 THBS1 Tissue inhibitor of metalloproteinase 1* TIMP1 10 The large dynamic range of the tear sample...
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System level modeling of endothelial permeability pathway and high throughput data analysis for disease biomarker selection
... pathways, the different components and the interacting partners, the kinetic parameters, the inhibitors and the activating factors Most of the information is obtained either from the 11 Chapter Introdcution ... medulloblastoma, and hepatocellular carcinoma [14 1, 14 7, 15 8, 17 7, 18 0, 19 9, 203-206] While these signatures display high predictive accuracies, the highly unstable and patient-dependent nature of these ... 5. 3 Results and discussion 16 5 5. 3 .1 CNV calls 16 5 5. 3.2 Statistics of the selected predictor genes from Breast cancer dataset 16 6 5. 3.3 The function of the...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... control for the effect of DMSO used to solubilize SB4 3 15 42, an equivalent amount of DMSOs were added to the samples not supplemented with SB4 3 15 42 SB4 3 15 42 is a selective and potent inhibitor of the ... RNAi3 Control 50 45 40 35 30 25 20 15 10 300 cells replated RNAi1 RNAi3 Control Figure 11 ES cells with reduced Lefty2 had significantly reduced capacity to form colonies when replated onto feeders, ... was then performed in joint effort with my colleagues to further narrow down the choice of candidate genes This thesis documents only the candidate genes for which I was solely responsible for the...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... The primary aim of this project hence serves to identify and delineate the roles of additional factors and pathways that are important for the maintenance of ES cell self-renewal To achieve this ... cell differentiation specifically towards the meso/mesendodermal cell fates The next objective to be met was the dissection of the molecular mechanism via which Lefty2 operates to maintain ES cell ... of Activin A ligand causes differentiation of ES cells into meso/mesendodermal lineages while repression of signaling with the specific chemical inhibitor SB4 3 15 42 and Lefty protein supplementation...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... SC-7966 1 /10 00 Mouse ab57 31 SC -16 16 1 /10 00 1 / 250 0 Rabbit Goat SC-8628 1/ 2000 Goat AM392-5M 1/ 200 Mouse MS -17 41- S0 1 /10 0 Mouse ab30 955 -10 0 P/N46-07 05 AF1 3 15 SC -17 743 1/ 50 0 1/ 50 00 1 / 250 1/ 200 Rabbit ... Chemicon ab57 31 Abcam ab 155 80 Santa Cruz SC -16 16 Biotechnology BioGenex AM392-5M DILUTION RAISED IN 1 /10 00 1 /10 00 1 / 250 0 Rabbit Rabbit Goat 1/ 200 Mouse Thermo Scientific 1 /10 0 Mouse SECONDARY ANTIBODY ... Santa Cruz 19 ) Biotechnology PCNA Santa Cruz Biotechnology CATALOGUE NUMBER 56 6 4 15 ab5487 -50 51 - 1300 DILUTION RAISED IN 1/ 50 0 1/ 50 0 1 /10 00 Rabbit Rabbit Rabbit 9 51 4 S 1 /10 00 Rabbit SC-6200 1/ 200...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... midline formation during vertebrate embryogenesis Development 13 2, 55 39 -55 51 Ciardiello, F., Dono, R., Kim, N., Persico, M G., and Salomon, D S (19 91a) Expression of cripto, a novel gene of the epidermal ... patterning the mouse embryo Development 12 8, 18 31- 1843 18 7 Lu, C C., and Robertson, E J (2004) Multiple roles for Nodal in the epiblast of the mouse embryo in the establishment of anterior-posterior ... asymmetry in the mouse Genes Dev 11 , 18 12 -18 26 Pease, S., and Williams, R L (19 90) Formation of germ-line chimeras from embryonic stem cells maintained with recombinant leukemia inhibitory factor Exp...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... embryo into its extraembryonic and embryonic components The outer layer of cells of the blastocyst is the extraembryonic components and forms an epithelium, the trophectoderm Descendents of the trophectoderm ... cytoplasmic tail with a homology to the gp130 The LIF/LIFR complex then recruits gp130 to form a trimeric complex (Zhang et al., 19 97) Heterodimer formation of the LIF receptor and gp130 receptor then results ... Descendents of the trophectoderm are restricted to the generation of the trophoblast components of the placenta The embryonic component, located on the interior is referred to as the inner cell mass...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... 11 2.8 90.8 10 6.2 7.8 4.2 11 4.4 15 9.8 13 .8 12 7 .5 0.2 0 .5 62.3 64 .1 159 .8 96.4 12 3.7 10 3.0 16 4.0 267.6 31 84.4 10 2.2 11 8.0 11 7 .1 229.7 10 3.2 2 05. 0 2 41. 9 12 1 .1 182 .5 17 9.4 12 2 .5 326.8 339.7 14 3 .1 ... 15 8.6 15 7.6 262 .1 2 15 .8 999 .5 12 85. 7 392.6 640.8 762.2 12 91. 2 226 .1 117 .7 13 0 .5 16 0.8 274.8 10 7 .1 440 .5 12 26.9 746.4 3 45 .1 75. 8 92.4 17 2.3 11 9.0 660.0 3768.6 39.8 283.4 53 3.8 294.2 11 8.9 16 4 .5 14 2 .5 ... 12 5. 0 23.0 5. 8 89.3 34. 8 98.8 67.6 80.0 54 .5 81. 0 12 .7 77.7 44.8 58 .2 96 .1 46.8 19 .8 44.8 7 .1 42.3 13 4.9 65. 3 10 7.8 10 1.6 30.3 10 8.8 29.6 33.3 57 .1 73 .5 55. 7 83 .1 16.8 23.0 78.4 16 9.4 10 8 .5 56.3...
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AN ANALYSIS ON THE DISYLLABIC TONE SANDHI OF CHONGQING DIALECT FROM THE PERSPECTIVE OF WORD STRESS AND TONAL COARTICULATION
... 位置 [T1]T1 [ 35] +[ 35] → [34] +[44] [T1]T1 [ 35] +[ 35] → [34] +[44] [T1]T2 [ 35] +[ 31] → [ 35] +[42] [T2]T1 [ 31] +[ 35] → [32]+[33] [T1]T3 [ 35] +[3 41] → [34] +[42] [T3]T1 [3 41] +[ 35] → [33]+[33] [T1]T4 [ 35] +[ 213 ] ... [ 35] +[3 41] → T3[T1] [3 41] +[ 35] → [33]+ [34] T1[T3] [34] +[332] [3 41] +[ 31] → T3[T2] [ 31] +[3 41] → T2[T3] [ 45] +[32] [33]+[332] [3 41] +[3 41] → [3 41] +[3 41] → T3[T3] T3[T3] [33]+[332] [33]+[332] [3 41] +[ 213 ]→ ... 上声 巴 1 [55 ] [ 31] [42] 去声 [ 25] ([ 35 ]) 杨 时 逢 (19 84) [ 45] 江北 （ [55 ]） [ 31] [42] [24] 甄尚灵、郝锡炯、 重庆 [55 ] [ 21] [42] [ 214 ] 黄 雪 贞 （ 19 86） 重庆 [55 ] [ 11 ] [42] [24] 曾 晓 渝 （ 19 96） 重庆 [55 ] [ 31] [ 41] [ 213 ]...
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Identification of plant as a novel and alternative host model for burkholderia pseudomallei
... symptoms; 3: 26 to 50 % with symptoms; 4: 51 to 75% with symptoms; 5: 76 to 10 0% with symptoms or dead) (Tans-Kersten et al., 20 01) Twelve to twenty plantlets were infected with each strain in each ... data for the infection of tomato plants with 11 4 B pseudomallei KHW∆TTSS3 mutant (Daily Disease Score) VI Accumulative data for the infection of tomato plants with 11 5 B pseudomallei KHW∆TTSS1/2 ... inoculating the bacteria into the medium The plantlets were maintained at 24 - 25 oC, with shaking at 10 0 rpm under a photoperiod of 16 hours light, hours dark The plantlets were observed for seven days for...
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Identification of pluripotency genes in the fish medaka
... water to 10 µl The tube was incubated at room temperature for 15 Then the DNase I was inactivated by µl of 25 mM EDTA solution to the reaction mixture, followed by 10 heating at 65 C Now the total ... lysate to stop lysis reaction After 50 μl of chloroform was added, the eppendorf tube was put on ice for more than Then, the bacterial lysate was centrifuged at 15 000 rpm for at 4°C The supernatant ... supernatant was transferred into a new eppendorf tube An equal volume of 10 0% isopropanol was added to precipitate the plasmid DNA The mixture was incubated on ice for 10 and centrifuged at 15 000 rpm for...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25