gfp aequorin probes expressed in cell cultures and detection of photon emission
Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
... Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating the expression of exogenous occludin, were located in the cell membranes In OccDE9-overexpressing cells, ... the bottom of the Matrigel-coated inserts The number of invasive cells was determined by counting the number of stained cells and then normalizing it to the number of control (Cont) cells (B) ... examined first [20] We observed that the intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular...
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Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx
... cases, intense staining of the granulation tissue in the sublining interstitium was evident Staining of the granulation tissue and lymph aggregates (red arrow) is magnified (Eb) Synovial lining cells ... staining for sPLA2-IIA (Fig 2A), whereas the enzyme was intensely expressed in synovial lining cells in the section from a patient with active RA (Fig 2B,C) Staining of the synovial sublining ... sPLA2IIE is induced rather specifically in VSMC in synovial tissues with active RA In inactive RA sections, sPLA2-IIF showed sporadic and weak staining in individual cells (Fig 4Ca,b), and a few interstitial...
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Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt
... represents an intermediate in processing These findings indicate that the processing of pro-CPA occurs in (at least) two steps, and that the processing of the intermediate form of CPA to mature ... dilution in NaCl ⁄ Pi) Staining was performed with a standard protocol using the biotin–avidin-based Vectastain Elite kit (Vector Laboratories) and diaminobenzadine (DAB) for detection of the secondary ... expression of mast cell granule serine proteinases and their role in inflammation in the lung and gut Immunology 105, 375–390 Caughey G (2001) New developments in the genetics and activation of mast cell...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of input proteins ... host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins [22–25] However, it is not clear at this point ... synthesis in tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells, physical observation revealed that cell death occurred in a...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... and was assisted in these experiments by JAB and JAAG MM and CR helped in designing appropriate Muc16 primers JC assisted in obtaining and maintaining murine ovarian tumor cells MK assisted in ... and intracellular Muc16 in the four MOVCAR cell lines was determined by flow cytometry using the 618 F antibody The mean fluorescence intensity for the binding of 618F to the cell surface and intracellular ... important for binding of the ovarian tumor cells to the mesothelial cells that line the peritoneal cavity Since the MOVCAR cells shed Muc16, this murine model may be important in understanding the immunomodulatory...
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ADAM10 is expressed in human podocytes and found in urinary vesicles of patients with glomerular kidney diseases pptx
... 3D) of podocyte specific marker proteins (integrin α3β1 or podocin) we confirmed that cells isolated from the urine are podocytes In addition, by intracellular FACS staining using ADAM10 and ... the involvement of the Notch pathway in the development of glomerular disease [15] In summary our finding that ADAM10 is expressed in podocytes and found in elevated levels in the urine of patients ... Podocyte injury occur in many glomerular diseases [21] To injure podocytes we treated the cells with different concentrations of puromycin Interestingly, increasing amounts of puromycin induced...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
... which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation of IRDy800CW-MNPs ... for imaging centered at 800 nm, which can be applied to in vitro cell labeling and in vivo imaging Methods Cells, animals and chemicals The RAW264.7 cell line was purchased from the China Center ... the same exciting intensity After housing and imaging for days following the injection of nanoparticles, the mouse was sacrificed by an overdose of anesthesia and the organs, including the heart,...
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Báo cáo y học: "The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae" doc
... representing bronchial epithelial cells (BEC) we found intense positive staining of these cells targeting NTHI infection in vivo and in vitro using ISH (figure 1d and 2c) In lung tissues of patients ... http://respiratory-research.com/content/11/1/67 Page of infection rate (determined by evaluation of positive ISHstaining) of 40-50% in AM and 35-45% in alveolar epithelial cells (AEC) was observed in infected COPD lungs (figure 1) In contrast, ... expressed and regulated by NTHI in the human lung This finding may be important for the understanding of inflammatory mechanisms and remodeling in COPD patients The combination of enhanced proinflammatory...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... assessed for morphology and cellular infiltration using haematoxylin and eosin (H&E) staining The degree of cellular infiltration was scored as described previously [6] Inflammatory changes were ... both activating and inhibitory FcRs, and the balance between activating and inhibitory signaling will determine whether uptake of immune complexes results in naive T cell activation and protective ... thickness of small airway walls and the number of infiltrated eosinophils around the airway increased, eosinophils appeared within the lumen of the airway, and goblet cell hyperplasia and hypertrophy...
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Development of a Regional Risk Management Framework for APEC Economies for use in the Control and Prevention of Introduced Marine Pests
... Within APEC little is known on the introduction of marine pests Little practical information on measures to prevent introductions Lack of knowledge in what individual countries are doing ... water and hull fouling are the most important vectors International shipping, aquaculture and biodiversity are most threatened values Amount of commercial shipping and number of trading partners ... enhance the effectiveness of existing instruments within APEC Institutional arrangements for managing the marine environment is fragmented in most economies Baseline surveys to identify IMP...
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Tài liệu Nanotechnology in Cancer Treatment and Detection doc
... smaller than most human cells Cancer Nanotechnology research is interdisciplinary and incorporates Biology, Chemistry, Engineering, Medicine, and Physics Properties of Cancer Cells • Epidermal Growth ... Noojin, Larry E Estlack, Robert J Thomas, and Benjamin A Rockwell Determination of threshold average temperature for cell death in an in vitro retinal model using thermograph Proc SPIE 7175, 71750G ... affinity to Cancer cells than healthy cells (EGFR binding) White light and simple, inexpensive microscope is all that’s necessary for powerful ex vivo Cancer detection The scattering is so strong...
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Tài liệu The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States: A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices docx
... resurgence in the incidence of TB occurred in the United States and included increases in the number of TB cases among adults and children and outbreaks of multidrug-resistant TB (MDR-TB) involving ... of skin testing is the Mantoux tuberculin skin test using 0.1 mL of tuberculin units (TU) of purified protein derivative (PPD) (1 ) BCG vaccination contributes to the prevention and control of ... preventing pulmonary TB in adolescents and adults These studies also were not useful in determining a) the efficacy of BCG vaccine in HCWs or b) the effects on efficacy of the vaccine strain administered...
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Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... Localization of silicatein in spicules and in intra- and extracellular vesicles by TEM immunogold labeling (i) Association of silicatein with spicules (A) Strong antibody reactions are seen within the ... been determined; the catalytic triad is histidine (His), asparagine (Asn) and serine (Ser) [4,12] In the active center of silicatein, the hydroxyl group of Ser26 and the imidazole of His165 (catalytic ... M & Hanada K (1982) l-transEpoxysuccinyl-leucylamido(4-guanidino)butane and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L Biochem J 201, 189–198 25 Muller...
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Tài liệu Trends in the Fees and expenses of Mutual Funds, 2010 pptx
... basis points in 1990 to 26 basis points in 2010 How ICI Measures Average Mutual Fund Fees and Expenses Investors in mutual funds incur two primary kinds of fees and expenses: sales loads and fund ... basis points of the 11 basis-point decline in the average expense ratio of funds of funds over the period (labeled in Figure 13 as “Fall in expense ratios of individual funds”) By definition, ... declined basis points in 2010, to 95 basis points (Figure 2) This decline was the result of a basis-point drop in load fees paid by stock fund investors, combined with a basis-point fall in the average...
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Tài liệu Trends in the Expenses and Fees of Mutual Funds, 2011 ppt
... declined in 2011 owing to declines in the expense ratios of individual funds, an increase in the demand for index funds, and a continuing shift by investors in both actively managed and index ... bundle in the fund’s expense ratio the cost of compensating financial professionals who may assist fund investors, whereas index fund investors who seek the assistance of financial professionals ... that invest in a mix of stock, bond, and hybrid mutual funds ICI RESEARCH PERSPECTIVE, VOL 18, NO | APRIL 2012 Much of the growth in funds of funds stems from investor interest in lifestyle and...
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Tài liệu Rubber plantation performance in the Northeast and East of Thailand in relation to environmental conditions docx
... processing and marketing through providing improved varieties of rubber seedlings, aiding in the establishment of both new plantations and replantings and providing technology and guidance (Chaninthornsongkhla ... expected land scarcity caused by outside land claims provides incentives for securing future land rights by forest clearing and rubber planting, and not so much for intensification of existing farming ... in Thailand, and the University of Helsinki This study was also a joint undertaking in the long series of academic collaboration between the universities of Kasetsart and Helsinki 1.2 Scope and...
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Tài liệu ‘Unheard voices’: listening to Refugees and Asylum seekers in the planning and delivery of mental health service provision in London. pptx
... about difficult things during this period User involvement User involvement was discussed both in terms of involvement in the planning and delivery of services and in terms of direct involvement or ... Reductions in disproportionate inpatient admissions Compulsory detention Use of seclusion Interpretation and investigation of violent incidents Monitoring and investigating death in mental health ... inequalities between black and minority ethnic groups and the majority white population in rates of mental ill health and equality of service in terms of experience and outcomes has figured in...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... 419, 420, 422 and 428 aa; nine aa in total) were located near the interface of the physiological dimer The Cterminal domain may contain structural features involved in protein–protein interactions ... basis of their crystal structures and the conserved amino acid residues (aa) of their active sites, these glutaminases are classified into a large group of bacterial penicillin-binding proteins and ... structure of salt-tolerant glutaminase Fig Active sites of Mglu and Bacillus glutaminase in the presence and absence of ligands Backbone atoms of the seven conserved amino acid residues and the...
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Gentle Measures in the Management and Training of the Young docx
... faculties of reason and reflection, and the power of appreciating "the necessity and propriety of things," and of bringing considerations of future, remote, and perhaps contingent good and evil ... denunciations of the heinousness of the sin of falsehood, with solemn premonitions of the awful consequences of it, in this life and in that to come, intended to awaken feelings of alarm and distress in ... them into right ways, and bring them out successfully in the end They will soon acquire the habit of never threatening, of never punishing in anger, and of calmly considering, in the case of the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25