further functional characterisation of the d6 n terminus
MOLECULAR AND FUNCTIONAL CHARACTERISATION OF THE ROLE OF HYDROGEN SULPHIDE IN SEXUAL MEDICINE
... xii NaHS sodium hydrosulphide hydrate NANC non-adrenergic non-cholinergic nNOS neuronal nitric oxide synthase NO nitric oxide NO2- nitrite NO2 nitrogen dioxide NO3 nitrogen trioxide NO3- nitrate ... and rate of NO metabolism in the body depends on several factors, including the concentration of NO itself, its diffusibility and the surrounding concentration of other bioreactants Having a neutral ... determine the concentration of cXMP in the sample Protein concentration in each 32 sample was quantified using Nanodrop (ND-1000, Nanodrop Technologies) The cGMP and/or cAMP concentration in each...
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Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf
... in the fibrinogen + thrombin reaction was investigated at a final concentration of fibrinogen of 0.1 mgÆmL)1 and a final concentration of thrombin of 0.4 NIH unitsÆmL)1 in the same polymerization ... projections) of the intermolecular connection between the D-domain of one fibrin desAA molecule (blue) and Bb12–46 (magenta) of another The model was prepared with PYMOL [24] on the basis of the X-ray analysis ... Bb12–26 and Bb26– 46 retard the stage of fibrin protofibril formation Discussion The N- terminal fragment of the fibrin b-chain contains regions related to the polymerization process and to binding of...
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Báo cáo y học: "Phenotypic and functional characterisation of CD4+ memory T cells homing to the joints in juvenile idiopathic arthritis" pps
... antigen-dependent or antigen-independent mechanisms In fact, cytokines produced in the synovial microenvironment (namely interleukin-7 and interleukin-15) might allow the proliferation, expansion ... activity and treatment at the moment of sampling Expression of CCL21 in SF and synovial tissue To gain further insight into the relevance of the interactions between CCR7 and its ligand CCL21 in vivo, ... extent, in the perivascular infiltrates of sublining layer and in the T and B cell aggregates These findings in synovial tissue are in line with the results of the phenotypic characterisation of...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... through the Ontario Genomics Institute, GlaxoSmithKline, Karolinska Institutet, the Knut and Alice Wallenberg Foundation, the Ontario Innovation Trust, the Ontario Ministry for Research and Innovation, ... main chain carbonyl Binding of a xanthine base from XMP in the same position, would lead to the loss of a hydrogen bond and a less favorable interaction To investigate this further, the protein ... phosphoribosyltransferase domain containing protein (PRTFDC1) is present in the human genome A recent study identified this homologue as a gene potentially involved in the development of ovarian cancer In a...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... mixtures containing only the indicated (d)NTPs in addition to the DNA template and the protein, whereas lane contains a control reaction without protein when ribonucleotides were included in the reaction ... et al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol ... activity on metal ions The results are the means of three independent experiments (C) The dependence of polymerase activity on the temperature was determined by assaying the enzyme in the standard...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... an increase in luciferase gene expression whereas the N- terminal region, containing the bHLH domain, is not essential for the interaction Dominant-negative DEC1 counteracts the suppression of ... cloned into pCR-Blunt II-TOPO (Invitrogen) The cDNAs thus obtained were confirmed by nucleotide sequencing and then subcloned into the expression vector pcDNA3.1/Zeo (Invitrogen) or the mammalian ... that the bHLH region, including His57 and Arg65, is responsible for the E-box binding Determination of the region in BMAL1 for binding to DEC1 To identify the region in BMAL1 required for the binding...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... protruding between helices and at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19] Detailed understanding of the functional role of these interactions ... conformation of the junction, then the deletion of this motif would have an even more serious effect on Ydc2 binding in the presence of divalent cations We tested the effect of Mg2+ on the binding of the ... in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure of the junction bound...
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Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx
... domain in the centre of the molecule A third domain in the N- terminal region before the Cu-binding domain also has a strong influence on activity Further analysis indicates that the C terminus influences ... and 45 prevented the binding of another monoclonal antibody that reacts with a conformational epitope in the C terminus [52] Analysis of the evolutionary conservation of the prion protein among ... indicated that the conformation of the C terminus is important to the activity of the protein Deletion of the whole C terminus rendered the protein inactive but surprisingly deletion of only the last...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... evidence in support of this hypothesis are meager In the head-to-tail orientation, the catalytic site in the N- terminal domain of one subunit and the substrate recognition site in the C-terminal ... center, the critical sites for heparin binding, and the orientation of dimerization Investigation into the structure–function relationships of LPL continues to provide important information for understanding ... of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain is increasingly appreciated,...
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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx
... deletion of either component of the import machinery significantly decreased the amount of peroxins of the importomer pulled down by the AAA peroxins, whereas the amount of components of the AAA ... Most interestingly, in contrast with the lack of other components of the peroxisomal protein import machinery, the deficiency of Pex4p does not interfere with the assembly of the components of the ... ⁄ NotI into the transcription activation domain containing plasmid pPC86 Recombinant DNA techniques, including enzymatic modification of DNA, fragment purification, bacterial transformation and...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... demonstrate the inverse importance of D1 and D2 in Pex1p and Pex6p for the interaction of these two proteins While a fragment of Pex6p consisting of N and D1 alone exhibits significant binding ... 53 Domain function of Pex1p and Pex6p or second, that the contact site is contained within D1 but binding is enhanced via conformational changes in the presence of N or D2 In analogy to NSF (see ... attention to the cassette structure of the proteins We assigned the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... the detergent partitions between the solution, protein and membrane, but only the concentration in the membrane determines the outcome of reconstitution Therefore, an advantage of the resonance ... concentrations and not give quantitative information on the concentration of detergent present in the membrane As expression of proteins in mammalian cells leads to the isolation of lg rather than mg of ... resulting XhoI/ApaI fragment was cloned into the corresponding sites in the polylinker region of the pcDNA3.1myc-His expression vector (Invitrogen, Groningen, the Netherlands) in order to obtain a...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... 1955 The understanding of the ne tuning of MGP gene expression requires further investigation and the use of different vertebrate systems may be useful in bringing new insights into the matter of ... to further analyze MGP gene expression In this report we present the cloning and organization of the MGP gene from X laevis and the functional characterization of its 5Â promoter region In transient ... corresponding introns of the mouse and human genes The consensus polyadenylation signal AATAAA is located in the 3Â UTR at nucleotide +8049 Genomic Southern analysis using EcoRI restriction digestion...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... amount of both, with a significant reduction of the b-phycocyanin component In contrast, the other three bands contain essentially a- and b-phycocyanin in stoichiometric amounts Injection of band ... chromatogram abundant in hydrophilic proteins such as linkers and a significant amount of a- and b-phycocyanins, and only traces of the allophycocyanins On the contrary, HPLC analysis of bands 2–5 revealed ... from each other by the percentage and type of bilin proteins they contain In order to study the influence of certain environmental changes on the supramolecular organization of the 1538 L Zolla...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... showing no significant differences in the distances of reacting atoms The only noted difference was in the orientation of the aromatic ring of pNPGlc in the F193A mutant (Fig 6D), owing to lost van ... obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was compared with the sequences of 22 other members of the ... an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type and one of the mutants The results provide indications...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... protein in the presence of the nucleotide These experiments indicate that the binding and/or hydrolysis of ATP not have any effect on the conformation of PfPDO, possibly because of the localization ... the double mutant was completely inactive, demonstrating the predominant role of the redox site at the C -terminus in the oxidative activity The action of PfPDO in catalysing interchange of intramolecular ... is present at the N- terminal half of the protein The PfPDO crystal structure provides some intriguing challenges to the understanding of the enzyme’s function [9–11] The protein consists of two...
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Báo cáo khóa học: Functional characterization of the evolutionarily divergent fern plastocyanin potx
... constant (kobs) on donor protein concentration (upper) and ionic strength (lower) for fern PSI reduction by fern (s) and spinach (d) Pc In case of spinach Pc, the kobs values correspond to the ... for the electron transfer from Cyt to Pc depend linearly upon Pc concentration As an example, Fig (upper panel) shows the linear protein concentration dependence observed for the electron transfer ... together, all of these data clearly demonstrate that fern PSI behaves as spinach PSI Fig Dependence of the observed rate constant (kobs) on donor protein concentration (upper) and ionic strength...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... after the noncovalent incorporation of the ligand Binding of DAUDA to Ag-NPA-1 caused 20% quenching of the emission of Trp17 indicating FRET between the single Trp residue and the dansyl group ... In addition to the protein peaks, they also contained IAEDANS or IANBD bands, indicative of covalent binding of the dyes [19] Binding activities of Ag-NPA-1 Binding of fatty acids, retinol and ... additional stabilization of the Ag-NPA-1 conformation upon ligand binding Role of Cys residues for Ag-NPA-1 conformation and function -1 According to the amino acid sequence, Ag-NPA-1 contains two...
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Báo cáo khoa học: ERS1 encodes a functional homologue of the human lysosomal cystine transporter pptx
... examine whether or not it can be complemented by CTNS The human CTNS gene contains 11 introns To analyse complementation of ers1D in yeast, the human CTNS cDNA was cloned into a yeast expression ... confirm the functional conservation between ERS1 and CTNS, a series of mutant CTNS genes was constructed, and their functional activities were monitored by the complementation of hygB sensitive ... state levels of cystinosin was the deletion of the N- terminal 121 amino acids This domain is predicted to face the lumen and contains all of the sites for N- linked glycan addition Consistent with...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... in ionic currents induced by coexpression of the human b1B could result from an increase in the number of channels present in the membrane, No, an increase in the probability of opening of the ... channels, Po, and/or an increase in the single channel conductance, co Discrimination among these options, however, requires evaluation of single channel parameters by other means (single channel ... that the human b1B subunit may be another candidate useful for studying the mechanism of upregulation of functional sodium channels on the cell surface and increasing the rate of spontaneous firing...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25