fourier transform infrared spectroscopy of intrinsically disordered proteins measurement procedures and data analyses
STRUCTURE ANALYSIS OF POLYSACCHARIDE ESTERS
... integrals of the protons of the AGU and the methyl protons of the propionyl moiety can be applied to calculate both the partial and the overall DS values of the acetate by means of Eqs (8.2) and (8.3) ... determination of polysaccharide esters [385] UV/Vis spectroscopy is usually applied after ester hydrolysis For calibration, standard mixtures of the polysaccharide and the acid are prepared and measurements ... can often not be analysed by “standard methods” and this has required the development of new analytical tools Most of the structural features of the polymer backbone are accessible via optical spectroscopy, ...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... moiety of Arg51 and three atoms of FMN (N1, O2 and O3) In both FeR and M acetivorans avoredoxin, the corresponding residue is asparagine (Asn47 and Asn52, respectively) to which only the O2 atom of ... the Km and kcat values of E coli Fre for riboavin are reported as 2.5 lm and 52.4 s)1 with NADPH as the electron donor and 1.3 lm and 30.6 s)1 with NADH [13] For FMN, Km and kcat values of E coli ... In the case of A vinelandii ferric reductase, the enzyme was puried as a mixture of two proteins or a heterodimer with molecular masses of 44 600 and 69 000 Da [25] Although none of the amino...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... to that of SGCI Both the amide proton of Lys13 of SGCI and of SGCI[L30R, K31M] variant and Asp13 of SGTI are quicklyexchanging, the corresponding signal can be hardly observed even after of incubation ... Ala26 and Cys27 These data together with the calculated structures are indicative of a number of intersheet hydrogen bonds in both of the molecules The exchanging properties of the amide protons of ... peaks between residues of the binding loop and that of the 11±16 loop are also different from that of SGCI and its variant (Table 3) Qualitative evaluation of H±D exchange data Proton±deuterium...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... )1 V and the anode potential was 10 V Both end-plate potentials of the ion trap were set at 1.5 V and the duration of the electron pulse was 100 ms Data acquisition and handling Primary data ... XMASSTM software (Bruker Daltonics) In the direct infusion experiment, a spectrum of 200 scans, 512 K data points was collected The spectrum was calibrated using a dataset of a sample of standard ... spectrum of the extract from pancreatic islets (a), showing details of the insulin and (b) and C-peptides and (c) signals The most abundant peptides turned out to be those from proinsulin Insulin and...
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báo cáo hóa học:" Growth and structure analysis of tungsten oxide nanorods using environmental TEM" doc
... layer on the wire and the behavior of the interface between the nanorods and wire due to the difficulty of the transmission of electrons for TEM analyses In this case, a part of the tungsten wire ... (a), TEM image (b), and SAED pattern (c) of the tungsten oxide nanorod Figure TEM of tungsten nanowire and HRTEM and TEM images of the nanorod (a) TEM image around the surface of non-heated tungsten ... diameters of about 10 to 20 nm and various lengths were confirmed in the TEM image in Figure 2a Oxygen and tungsten mappings of Figure 2a area are shown in Figure 2b, c The existence of tungsten and...
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Báo cáo y học: "Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation" pot
... binding proteins, transcription factors and ribosomal proteins, and comprise only 10.4% of probe sets on the microarray Figures and show the distribution of gene functions in the upregulated and ... expression levels of Sox2, Sox4, and Sox11 mRNA mRNA expression levels of Sox2, Sox4, and Sox11 Shown are mRNA expression levels of the oligodendrocyte precursor genes Sox2, Sox4, and Sox11 in embryonic ... by NFI-A in axonal growth and guidance deposited research Figure Validation of microarray data by qRT-PCR Validation of microarray data by qRT-PCR The mean fold changes of mRNA expression in postnatal...
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Crystal structure analysis of pils, a type lvb pilin from salmonella typhi
... values of h, k, and l Eq 1.17 is known as the electron density equation 1.5.2 Fourier transform If two mathematical functions exist, say f and g, in a way that g is the Fourier transform of f, ... the native data and the derivative data sets, upon incorporation of heavy atoms into the crystals The first step in this method requires attachment of heavy atoms and the determination of the coordinates ... very important that the observed data be split off at the very beginning of structure refinement into a working set (95% of reflections) and a free set (5% of data) As the refinement proceeds,...
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A study on the structure of the speech “ I have a dream” by Martin Luther King A systemic functional grammar analysis
... analysis of the speech “I have a dream” Chapter IV: Conclusion summarizes the outcome of the research and suggests some further implications for language teaching and learning References Alexander, ... teaching and learning 1.3 Scope of the study It would be impossible for the researcher to cover the issues of FG However, some important notions such as linguistic system, metafunctions and cohesion ... Methodology of the study The structure and meaning of the speech will be focused in the study, therefore the methodologies applied are: Descriptive which is used for illustrating the crucial matters of...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, NO2-, and ... 2004 EXPERIMENTAL MATERIALS AND METHODS Biofilm Samples Two types of biofilms, a domestic wastewater biofilm and an autotrophic nitrifying biofilm, were studied Both biofilms were cultured in partially ... specific consumption and production rates of NH4+, NO2-, and NO3- The biofilm surface was at a depth of zero The values are means of triplicate measurements Microelectrode Measurements Steady-state...
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An Analysis of Domestic Water Demand Structure and Water Recycling in Beijing
... household at the end of the six-day survey for each day of the study period The questions were read before and after each period of water use, and the frequency and head count of water use were ... recycled Source: Statistical Yearbook of China (National Bureau of Statistics of China, 2005) and Beijing (Beijing Municipal Bureau of Statistics, 2005b), and data from sewerage plants In 2005, ... household data of water use for - 135 - Journal of Water and Environment Technology, Vol 9, No.2, 2011 showering, toilet flushing and cleaning were not available Structure of annual and seasonal...
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an economic analysis of financial structure
... hid information and engage in undesirable activities • Separation of ownership and control of the firm – Managers pursue personal benefits and power rather than the profitability of the firm Tools ... Conflicts of Interest: Remedies II • Sarbanes-Oxley Act of 2002 (cont’d) – Beefs up criminal charges for white-collar crime and obstruction of official investigations – Requires the CEO and CFO ... Conflicts of Interest: Remedies I • Sarbanes-Oxley Act of 2002 (Public Accounting Return and Investor Protection Act) – Increases supervisory oversight to monitor and prevent conflicts of interest...
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02 three dimensional static and dynamic analysis of structure
... verify the results of all analyses and assume professional responsibility for the results It is assumed that the reader has an understanding of statics, mechanics of solids, and elementary structural ... and are defined in terms of three numbers: modulus of elasticity E , Poisson’s ratio 1-2 STATIC AND DYNAMIC ANALYSIS ν and coefficient of thermal expansion α In addition, the unit weight w and ... trademark of Computers and Structures, Inc SAP90, SAP2000, SAFE, FLOOR and ETABS are trademarks of Computers and Structures, Inc ISBN 0-923907-00-9 STRUCTURAL ENGINEERING IS THE ART OF USING MATERIALS...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and ... building blocks of eukaryotic chromatin [15] A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been well documented and includes monomethylation and Fig ETD ... topic of ETD, see [30–34] Acknowledgements This work was supported by grants from the National Institutes of Health (GM37537 and U54 G64346 to D ETD-MS analysis of peptides and proteins F Hunt) and...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... superposition of the exponential rise to analyze quantitatively the contribution of each phase The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... excellent approximation of FI traces and to quantitatively estimate the contribution of each phase The average values of amplitudes and half-times (t1 2) found for each phase of the FI measured at ... contributions and kinetics of the three main components of the FI traces Deconvolution of the traces with the sum of three exponential rises provided an excellent t between simulated and experimental...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... presence of Mn2+ The midpoint of the Mg2+ titration curve in the absence of Mn2+ is approximately 4.5 mm, and nearly full activity is reached by 10 mm Mg2+ Hill analysis of the data gives n-values of ... P5, P6 and minor parts of P5a and P6a are protected Also, J4 is weakly protected, but J5 is weakly cleaved Of particular signicance is the observed cleavage of J6 and the neutrality of J3 ... three nucleotides of P7, and the 3Â-half of J8 7, are not protected The rst three nucleotides of P7 are part of the G binding site, as are the terminal nucleotides of J6 and J8 [3537]; the...
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Tài liệu Báo cáo khoa học: "Identifying Linguistic Structure in a Quantitative Analysis of Dialect Pronunciation" docx
... real data it is often the case that one sound corresponds to several other sounds and that sound correspondences involve series of segments In future work some kind of a feature representation of ... process of the aggregate analysis will be given 3.1 Data Set The data used in this research, as well as the research itself, are part of the project Buldialect—Measuring linguistic unity and diversity ... common technique in a statistical data analysis based on a partition of a set of objects into groups or clusters (Manning and Schütze, 1999) Multidimensional scaling is data analysis technique that...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... presence of two tandem patterns of cysteine residues constituting two internal three-finger shaped motifs typical of urokinase-type plasminogen activator receptor (u-PAR) and cell surface antigens of ... in Fig 2A From 2.5 g of serum proteins, mg of homogeneous DM64 were obtained Physicochemical and chemical characterization DM64 has a molecular mass of 63 659 Da by MALDITOF MS SDS/PAGE, under ... devoid of myotoxicity and cytotoxicity in these experimental systems DM64 did not inhibit enzymatic, lethal and anticoagulant activities of myotoxin I, even when a twofold molar excess of the...
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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... Electron microscopy of rotary shadowed proteins [34], CD, and matrix-assisted laser desorption ionization MS [35] followed standard protocols Construction of expression vectors and cell transfections ... small amount of secondary structure [41] Hexosamine analysis of b1IV monomer and dimer showed 5±6 residues of glucosamine and less than one galactosamine That Edman degradation of various proteolytic ... expected in front of C3 and C5 and should also contain C4 (Fig 1A) A smaller pepsin fragment showed a connection between C3 and C5 Yet because of the variable thiol content of C3 to C5 (see above),...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference ... resonances of Pro1 and Asp2 have the highest intensities in the STD spectrum, signals of Thr3 are of medium intensity, while the signals of Arg4 and Pro5 have the lowest intensity The d-protons of Pro5 ... STD and normal TOCSY spectrum of the PDTRP/SM3 complex are shown The peaks of Arg4 and Pro5 are so low in intensity that they not appear at the intensity cutoff shown Signals of Pro1, Asp2 and...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet ... type and various mutants, domains III, IV and V display a movement relative to domains I and II, resulting in a shift of the ˚ tip of domain IV of up to A [14,16] The ribosomebound structure of ... 666 and 670, within one b-strand of domain V, are also important for interdomain interactions The N-terminal part of the strand interacts with the N-terminal part of helix CG in domain G, and...
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