fluid waterlike basaltic lava flows that cover an extensive region in the northwestern united states known as the columbia plateau
báo cáo khoa học: " Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses" ppt
... MtCBF4 was analyzed using a yeast assay system The GAL4 DNA-binding domain-MtCBF4 recombinant plasmid was transformed into yeast cells and assayed for its ability to activate transcription of the ... underlined) An NcoI-SpeI fragment containing MtCBF4 cDNA was inserted into pCAMBIA1302 containing the 35S CaMV promoter and a hygromycin (kanamycin) resistance marker The plasmid was introduced into ... recombinant plasmid was then transferred into a yeast strain YRG-2 carrying the reporter genes His3 and LacZ The yeast strain cannot grow on the SD plates without histidine, which cannot induce...
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Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... However, an alternative isomerase that converts all-trans retinoids to 11-cis retinoids in the retina has not been identified in any species, and RPE65 remained as the only known isomerohydrolase that ... regeneration in the zebrafish eye [39] In that study, evidence was provided showing that there is another isomerohydrolase in the zebrafish retina and that it is RPE-independent Therefore, the present ... isomerohydrolase in the retina Y Takahashi et al thetase to esterify the 13cROL generated in the reaction Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig S3B) Under the in...
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... the c ring, respectively The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in the ... covalently linked hairpins in the membrane [10] Importantly, the ion-binding site is not conserved in hairpin one If one assumes the same number of transmembrane helices in V0 and F0, the rotor ... acid (lanes 6, and 9), and individual subunits were detected by silver staining (lane 6) or immunoblotting using an antibody against subunit c1 (lanes and 8) or subunit c2 ⁄ (lane 9) The antibody...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... contributing to these interfaces is located in the PYR and middle domains The interface contains 10 hydrogen bonds in IPDC The dimer–dimer interface in IPDC is smaller than the corresponding interface ... displayed as rectangles, b-strands as arrows ÔdÕ indicates residues in the dimer interface and ÔtÕ residues in tetramer interface Residues lining the active site cavity are underlined Residues binding ... of the corresponding intermediate in transketolase [37] and the model derived for ScPDC [38] (Fig 6) In the immediate vicinity of the modelled a-carbanion/enamine, there are a number of invariant...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... laser intensity was 2300, the pulsed delay time was 200 nsec, the accelerating voltage was 12 000 V, the grid voltage was 7200 V and the guide wire voltage was 9.6 V The instrument was used in the ... galA and by then cloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking region, in pIM4700 (containing the pkiA promoter) A niger The sugar ... encoding main chain cleaving enzymes as well as with the arabinanolytic genes [1] The absence of expression of galA in the presence of pectic galactan indicates that galactose is not an inducer...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... procedures), and the DdesA mutation was verified in one of the mutants by PCR and Southern blot analysis A 1462-bp PCR band corresponding to the extended resistance cassette was found only in the mutant ... supernatants of the DdesA mutant, whereas desferrioxamines B and E were produced in the parental strain (Fig 2) The DdesA mutant was able to grow on Streptomyces minimal medium containing l-lysine as ... band was obtained only in the complemented strain when a kan (kanamycin resistance) fragment (1519 bp) was used as probe, as expected (Fig 3C) Complementation of the desA deletion in the mutant...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... explain the KIR action is that it can mimic the activation loop found in kinases such as JAK2 and FGF receptor kinase [5] and prevent substrate access to the catalytic groove of the kinase [6] In ... of interacting with an E3–ubiquitin ligase complex by binding directly to elongins B and C [12], SOCS proteins can recruit bound signal transduction proteins, such as activated kinases or the ... phosphotyrosine binding but may have an important functional role Results hydrophilic in PtdIns 3-kinase but not in SOCS3 were identified and mutated to match the PtdIns 3-kinase residue The six mutants...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... determinants in their coding region [24–27] Moreover, in the case of c-fos, it was shown that ARE mediates mRNA deadenylation by a translationindependent mechanism, while the coding region instability ... ARE in this process [10,11] In addition, another destabilizing sequence was identified within the coding region of IFN-b mRNA [12,13] Whereas the independent removal of the ARE or the coding region ... (2001) Plasminogen activator inhibitor type contains mRNA instability elements within exon of the coding region Sequence homology to coding region instability determinants in other mRNAs J Biol...
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Báo cáo khoa học: A unique tetrameric structure of deer plasma haptoglobin – an evolutionary advantage in the Hp 2-2 phenotype with homogeneous structure pot
... size of the a-chain in whales and dolphins as well as other ruminants (cattle and goat) to determine the possible time of the tandem repeat evolution in deer Hp Interestingly, the inserted panel ... divergence of the line leading to ruminants and pig, as pig possesses only the Hp 1-1 phenotype [24] As dolphins and whales are the closest divergences before the ruminants, we further examined the size ... between the repeat regions of the human a2-chain is 96%, with only two amino acids mutated (replacement of Asn52 and Glu53 in the B region by Asp52 and Lys53 in the B1 region) This tandem repeat...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... c1 was unknown, and an N-terminal sequence of this fragment was not obtained Therefore, it is not only not excluded that it was the c1 subunit but likely, as the c ring is rather stable, and the ... of V1V0ATPases has only half the number of ion-binding sites This is seen as the reason for the apparent inability of V1V0-ATPases to catalyze ATP synthesis in vivo Indeed, V1V0-ATPases have ... blotted against c1 antibodies Lane 4: ATP synthase was incubated for 10 at 80 °C, and blotted against c1 antibodies Lane 5: the sample was incubated for 10 at 80 °C and hybridized with antibody...
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Báo cáo hóa học: " The complete genome sequence of a Crimean-Congo Hemorrhagic Fever virus isolated from an endemic region in Kosovo" pot
... East and Southern Russia and to the focal endemic areas in Africa and southern Europe, including Kosovo and Turkey [2] Yearly epidemics, as well as sporadic cases of CCHF are seen in some of these ... group The group V topology based on the M segment, was a little different (Fig 1, panel B) Two Russian strains (VLV-100 and Kashmanov) clustered together with the Turkish CCHFV whereas the Russian ... similar in the S and L segment tree (Fig 1, panels A and C), where it formed a separate lineage ancestral to the three Russian isolates and CCHFV strain 200310849 from Turkey was the most ancestral...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_1 docx
... criticize as well as please the client As outsiders they are much more objective and can often become the guardians of the client organizations reputation In addition they can cover many topics ... licence is being granted Any assignment must be in writing It can be in the form of a letter and must be signed by, or on behalf of, the assignor Licences can either be in writing or granted orally ... very important part in the work ethic of the organization and ignorance of it can adversely affect the consultancys relationships with the client Mixing and matching There are occasions when...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_2 pot
... misunderstandings and can also serve as the contract, or the basis of one Illustrations The same rules apply here as they with publishers Check with them first, as you need to know if they have any ... of the shoot with the photographer, such as timings, the hire or loan of props, hiring of any models, transport arrangements, etc You might want to use a studio setting for the job, either the ... building, or a building site Weather watching On the day, a weather watch may be needed if there is any question about the weather holding Delays mean money In the event of wet weather there should...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_3 doc
... service on the stand, so why so in an amateurish way? Bad planning and slipshod operating of an exhibition stand is bad public relations, and the organization being represented on the stand will ... a practical guide Planning the stand In planning what the stand will look like you can choose either to do your own thing by using existing material, designing your own stand with visual displays, ... Exhibitions and other events the detailed planning and also make the best use of its own resources, both in terms of time and money Used extensively by organizations and trade associations in the UK, they...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_5 docx
... principles remain the same These, then, form the infrastructure, or skeleton, for the management of a crisis situation Assess Look at your organization as objectively as possible Try and imagine ... cope, with any emergency situation that may arise in such a way that the minimum amount of damage is caused to the organization in whatever context that may be Any organization that has the misfortune ... food poisoning, a bomb at a mainline railway terminus, a fire in a shopping precinct These are the sorts of scenarios that could affect your organization and its reputation Think of it as the What...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_6 doc
... conversations the least accurate but sometimes indicative Analysis The analyst (that is the PRO) then has to examine all the material collected, analysing and classifying it so that a report can be drawn ... Part of the IPR definition of public relations includes the following: the deliberate, planned and sustained effort to establish and maintain mutual understanding between an organization and its ... issued by the Institute from time to time They are intended to assist members in interpreting the Code, but it must be emphasised that they cannot be all-embracing Circumstances can vary and it...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_7 ppt
... and take the initiative in offering advice and services The Client agrees to assist the Consultancy in the performance of these duties by making available to the Consultancy all relevant information ... as defining the scope of, rather than attempting to exclude, liability Insofar as it is possible to exclude any provision of the aforementioned or any other relevant act, the Consultancy and Client ... Termination The Client agrees to appoint the Consultancy for an initial period of After the initial period the appointment shall continue until either the Client or the Consultancy serves...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_8 pdf
... Costings The financial costs, any benefits arising and other details of relevant financial information This will be helpful to any decision making Any detailed breakdowns should be given in an ... signed and dated as a true record by the Chair All heading thereafter to be numbered sequentially.* Matters arising 2.1 Unfinished business Any business recorded in the minutes that has not been ... although the terms of the contract may cover this The task of distinguishing between the two types of contract has been left to the courts to decide in cases where there are doubts or problems Any...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice by David Phillips and Philip Young_9 doc
... to the media At the finish l l Stand down Stand down the crisis team/equipment, etc gradually as the situation eases Staffing levels can be reduced inside the centre Follow-up action There ... arrangements 54 confidential information 12021 consultancy 59 advantages of using and client relationship 117 disadvantages of using independent 78 mixing and matching reasons for using 67 types 78 ... Contingency Planning for a Crisis Before the crisis Identify Plan l l l l l l look at possible situations make simple contingency plans to cover different situations They should include: the...
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Online Public Relations A Practical Guide to Developing an Online Strategy in the World of Social Media PR in Practice_1 pptx
... physicians who trained in the 1930s might argue that the introduction of penicillin and other antibiotics in the 1940s might have done more to change the practice of medicine than any group of innovations ... on pointing out the disparity between where they were and where they wanted to be rather than comparing themselves with competing institutions, and they linked quality to more than simply clinical ... become increasingly complex AMCs can be composed of as many as four separate components: the hospital, the medical school, the university, and the physician practice plan The physician practice plan...
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