... % of glycolic acidand % of formic acid were detected in The amount of malic acid decreased, and was converted into more simple organic acids as reaction time increased Glycolic acid increased ... Effect of reaction time on the content change of products from DCAA and TCAA by o hydrothermal reaction at 250 C and MPa Journal of Water and Environment Technology, Vol.1, No.2, 2003 acid, % of ... partial destruction of MCAA by hydrolysis, production of biodegradable glycolic acidand decomposition of glycolic acid to other organic acids were reported [11] Based on the results of ion chromatography,...
... by standard statistical formulae (Ferguson, 1980) Ethanol and acetic -acid tolerance patterns of mass cultures of each of populations of D melanogaster were assessed following the procedure of ... ethanol and acetic acid The parallel utilisation of ethanol and acetic acid in Indian tropical and subtropical populations of D melanogaster concur with such data on temperate populations of D melanogaster ... for ADH (Harris and Hopkinson, 1976) The genetic control of ADH banding patterns was interpreted from the segregation patterns of enzyme electromorphs of l parents, F and F progeny of several single-pair...
... compared and displayed as Venn diagrams 10A compares GA and germination; 10B compares ABA and germination; and 10C shows the comparison of GA, ABA and germination The number of probe-sets and their ... amino acid metabolism amino acid metabolism.synthesis amino acid metabolism.synthesis.central amino acid metabolism amino acid metabolism.synthesis.central amino acid metabolism.GABA amino acid ... regulatory and signaling components, and therefore to initiate a variety of transcriptional regulatory cascades and signaling pathways involved in germination and seedling growth The bin of Cell Wall and...
... Proteomic analysis of the seeds of another woody plant, Prunus campanulata, provide evidence of the involvement of prunin and dehydrin in the response to warm and cold conditions of stratification, ... maintenance methylation and affecting expression of thousands of genes The hog1 mutant plants grow slowly and have low fertility and reduced seed germination Complementation of the hog1 mutation ... seeds Taken together, the expression of alpha- and beta-tubulins is the important determinant of completion of dormancy breaking and initiation of germination and growth Conclusion Temperate Norway...
... regulator of JNK and p38 MAP kinases in microglial cells exposed to LPS Blockade of MKP-1 expression by triptolide enhanced the phosphorylation of JNK and p38 MAPK pathways and the mRNA expression of ... distribution of actin and tubulin and lack membrane localization of these proteins and process formation (Eugenin et al 2005) When cells are treated with a chemotactic stimulus, rearrangement of actin and ... view of these observations, it is hypothesized that RA may modulate the inflammatory response and proliferation index of microglia Hence, we have investigated the effects of RA on release of proflammatory...
... evolution of all of the enzymes of the pathway (Fig 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring ... OGDH and by branched-chain a-oxoacid dehydrogenases (OADH) It is therefore not surprising that all three enzymes have an E1, E2, E3 subunit structure, and that some of the subunits of PDH, OGDH and ... mitochondria and glyoxysomes, respectively [42,43] They usually have a molecular mass of 90 kDa and are typically homodimers of 45-kDa subunits [44,45] In the presence of Mg2+, glyoxysomal CS of plants...
... Number of Coughs Number of Coughs 20 15 10 Pre-screen CitricAcid 25 Number of Coughs 25 25 V112220 (n=5) 20 15 10 Capsaicin Pre-screen CitricAcid Capsaicin 20 15 10 Pre-screen CitricAcid Capsaicin ... inhibition of cough induced by citricacidand capsaicin in the guinea-pig [14,15] Capsaicin CitricAcid 25 20 15 10 ** Control Vehicle n=4 n=5 V112220 n=5 Number of Coughs Number of Coughs 25 ... V112220 n=5 Figure Number 2of coughs following citricacid or capsaicin exposure Number of coughs following citricacid or capsaicin exposure Left panel show results from citricacid exposure while...
... fatty acids, and the ratio of each fatty acid RESULTS AND DISCUSSION Characteristics of carbon stable isotope in Z japonica The mean δ13C of Z japonica was -12.4 ± 1.6‰ in the leaves and stems, and ... leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of Z japonica , because we needed to exclude the influence of a ... characteristics of carbon stable isotope and fatty acid structure of Z japonica Furthermore, we compared the characteristics of carbon stable isotope and fatty acid structure of Z japonica with those of the...
... Reactivity of native (lines and 4), pH buffer-exposed (lines and 5) and pH 2.8 buffer-exposed (lines and 6) IVIg with Bacillus anthracis antigens The membranes were incubated with two concentrations of ... reactivity of IgG, we used real-time kinetic measurements of the interaction of IVIg with C-reactive protein (CRP) The binding profiles obtained after single injections of native andof low-pH ... prevents brisk changes in the levels of components of inflammation, coagulation, and other pathways [21] The administration of a single dose of 500 mgÆkg)1 of the modified IVIg had significant (P...
... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... bonds for Asn11(CO)–Trp14(HN) and Ser12(CO)– Thr15(HN), and confirmed by the slow solvent exchange kinetics of the amide protons of Trp14 and Thr15 The observation of two small 3JHN-Ha coupling...
... Asn210 and Ala276 are important for the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid ... described [5] Purification and characterization of Dfp and CoaB proteins Growth of strains E coli M15 (pREP4, pQE8/pQE12) cells were grown in the presence of ampicillin (100 lgÆmL)1) and kanamycin (25 ... and 450 nm Molecular mass information was obtained as described [6], determining the elution volumes of standard proteins and the void volume of the column The Superdex 200 PC 3.2/30 column and...
... (Keio University)’ and a grant from New Energy and Industrial Technology Development Organization (NEDO) of the Ministry of Economy, Trade and Industry of Japan (Development of a Technological ... volume of 100 lL Each assay contained 100 mm acetate ⁄ NaOH (pH 5.0) and 10 mm of substrate Assays were initiated by the addition of substrate, progressed for 10 at 37 °C, and terminated by lL of ... trichloroacetic acid After dilution with 100 lL of water, 25 lL of 2.5% ammonium molybdate in 2.5 m H2SO4 and 10 lL of Fiske–Subbarow reagent were added The mixtures were incubated for 20 at 37 °C and absorbance...
... hydroxide for 40 D-galactose, D-galactobiose and D-galactotetraose were used as standards to identify the D-galactose and D-galacto-oligosaccharides The calculated areas for D-galactose and D-galacto-oligosaccharides ... in the presence of D-galacturonic acid (Fig 3) However, in the CREA mutant expression of galA was detected in the presence of D-galacturonic acidand L-arabinose The expression of galA is very ... transformant and was used for the purification of GALA Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods Purity of the preparation...
... amino acids (i.e Q, N, S, and T) and an increase in charged amino -acid residues (i.e K, E, and R) in hyperthermophilic proteins As S and T can catalyze the deamination and backbone cleavage of Q and ... volume of blue dextran (X) Protein standards; (A) BLXI Linear regression (1), with an r of 0.951, is based on the elution data of all four protein standards Linear regression (2), with an r of 0.981, ... an r of 0.981, is based on the elution data of the 200, 150, and 66 kDa protein standards Linear regressions (1) and (2) give molecular masses of 200 and 177.5 kDa, respectively, for BLXI q FEBS...
... phosphonic and phosphinic acid derivatives of GSH as moderate Kinetics and inhibition of ATP-dependent ligases inhibitors of CfGspS [38] The most active of these was a phosphonic analogue of GSH ... auto-oxidation of NADPH and hydrolysis of ATP in the coupling system, the net rates of endogenous ATPase activity ($ 0.01% of kcat) in the presence and absence of inhibitor are 1.4 ( 0.9) ã 10)3 and 3.0 ... insertion of 17 amino acids in the amidase domain and two in the synthetase domain (one of 14 amino acids and the Fig Conservation of key functional residues identied for EcGspS in CfGspS and TryS...
... accounting for the 52% and 69% of the ECEC, respectively Soils S1 and S3 had the lowest ECEC, pHw and clay content while S2 and S4 were less acidic and showed higher ECEC and clay content Soil ... kaolinite and more smectite and illite Both S1 and S2 had similar proportions of phyllosilicates in the V mm soil fraction andof goethite and haematite in the clay fraction The greater content of illite ... intervals of the estimated exponents, and the standard error of the estimate are shown in Table The average value of the exponents found for S1, S2 and S3, were 0.03 F 0.015 (standard deviation) and...
... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, ... However, detection of E3 and lipoic acid in various archaea [10–12] led to the discovery of a putative OADHC operon in Haloferax volcanii [13] and our subsequent detection of similar putative ... oxidation of 2-oxoacids is catalyzed by an unrelated, and structurally more simple, family of 2-oxoacid ferredoxin oxidoreductases (FORs) This comprises the pyruvate FOR, the 2-oxoglutarate FOR and...
... regulation of GnRH II gene by RARs, TE671 and JEG-3 cells were treated with all-trans retinoic acid (ATRA; a ligand of RARs) and ⁄ or 9-cisRA (a ligand of RXRs) before the measurement of GnRH II ... vector Effect of unliganded RARa and RXRa and retinoic acid (RA) treatments on GnRH II promoter activity To investigate the in vivo effect of RARa and RXR on the transcriptional regulation of the hGnRH ... activity of the hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the promoter activity of the hGnRH II gene in TE671 cells and JEG-3...
... pregnancy Table Analyses of fatty acid ratio and relative contents of n-3 PUFAs EPA, DPA, and DHA in mammary glands Whole inguinal mammary fat pads were isolated and contents of fatty acids were analyzed ... favor of n-3 PUFA and particularly an n-3 DPA and EPA An increase in n-3 DPA and EPA, and perhaps other n-3 PUFAs, stimulates Jak2 and Stat5 activation, and induces b-casein expression and gland ... alternation of n-6 ⁄ n-3 compositional ratio in favor of n-3 fatty acidand a robust increase of n-3 DPA and EPA in the mammary gland following pregnancy indicate the potential role of the n-6...
... aromatic amino acidand X is a variable amino acid) typical of retroviral nucleocapsid proteins and the presence of an glycine ⁄ arginine rich region (RGG) box between the first and second Zn knuckles ... single-stranded nucleic acids and is able to promote interstrand reannealing of DNA as well as RNA [35,36] hnRNP Al is phosphorylated by CK2 and PKA PKA phosphorylation suppresses the ability of hnRNP ... composed of unpaired strands, CNBP, hnRNP K, and additional factors [7] Modulation of the concentrations and ⁄ or the biochemical activities of either CNBP or hnRNP K could influence the ability of...