fe2 binding activity was measured according to the method of simada et al 1992 briefly extracts 2 ml were mixed with hexamine buffer 10 mm 2 ml containing kcl 10 mm an
Folk medicine cratoxylum cochinchinense antioxidant but cytotoxic
... 54 2. 2.17 .2 Trypan blue exclusion assay 54 2. 2.18 Treatment of Jurkat T cells with YCT 55 2. 2.19 O2 electrode assay 55 2. 2 .20 Cell cycle analysis using flow cytometry 56 2. 2 .21 Annexin V-FITC and ... staining of Jurkat T cells 56 2. 2 .22 Release of lactate dehydrogenase 57 2. 2 .23 Assessment of cellular and nuclear morphology 57 2. 2 .24 Caspase and activities 58 2. 2 .25 Western blot analysis 59 2. 2 .26 ... (Lin et al. , 1995; Sindambiwe et al. , 1999; Duffy and Power, 20 01), antiviral (Wang, 20 00; Sindambiwe et al. , 1999), anti−inflammatory, antiallergic (Di Carlo et al. , 1999), immunosuppressive, immunostimulatory...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... USA) The Sep-Pak was flushed with 150 mL water and then 150 mL of 30% methanol to remove any remaining isoflavone glucosides The Sep-Pak was washed with 50% methanol (100 mL) to remove the group ... vitro sialytransferase activity (Wu, Hus et al 20 01) Hsu et al (Hsu, Lin et al 20 05) confirmed that soyasaponins I was an in vitro sialytransferease inhibitor and was found to decrease 2, 3-sialylations ... et al 20 06), other triterpenoids (Popovich and Kitts 20 04) and specifically group B soyasaponins (Berhow, Cantrell et al 20 02; Jin, Yang et al 20 06) Decroos et al (Decroos, Vincken et al 20 05)...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... P 010 726 -22 ; lane 4, P 010 726 -23 ; lane 5, P 010 726 -24 ; lane 6, P 010 726 -25 ; lane 7, P 010 726 -26 ; lane 8, E01 020 6-13 -2; lane 9, J 0103 03-11-1; lane 10, O157-R1-3 -2; lane 11, O157-C-1 -2; lane 12, ATCC ... Kim et al Table Results of multiplex PCR, H7 PCR, antiserum tests, motility test, and vero cell assay Isolates P 010 726 -18 P 010 726 -21 P 010 726 -22 P 010 726 -23 P 010 726 -24 P 010 726 -25 P 010 726 -26 E01 020 6-13 -2 ... P 010 726 -24 ; lane 6, P 010 726 -25 ; lane 7, P 010 726 -26 ; lane 8, E01 020 6-13 -2; lane 9, J 0103 03-11-1; lane 10, O157-R1-3 -2; lane 11, O157-C-1 -2; lane 12, A1; lane 13, A2; lane 14, A3; lane 15, A4 lanes...
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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... TCID50 of GPV was calculated as 10 /mL according to Reed-Muench’s method [1] The viral activity of GPV could be neutralized with CCV positive serum from dogs The mean neutralization titer was 2. 18, ... to the S protein of CCV K378 In addition, the sequence of the GPV S gene was also 84.3% to 100 % identical to the other strains of CCV, including CCV1-71 (AF11 624 6), CCV6 (A 228 82) , CCV C54 (A 228 86), ... PCR methods to identify a CCV The PCR product was cloned into pGEM-T Easy Vector (Promega, USA) per the manufacturer’s recommendations and then sequenced The sequence was analyzed by GENETYX...
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Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... S transferase (GST) Anti-oxidative activity of A paniculata contributes to its antiinflammatory, anti-cancer, anti-hepatotoxic, anti-atherosclerotic and anti-diabetic activities [27 ,44,91 ,108 ,117] ... 2. 89 2. 08 1 .29 2. 02 19-O-acetylanhydroandrographolide 2. 4 3.85 2. 75 2. 29 2. 08 14-deoxy-14,15-dehydroandrographolide 19-O-acetyl-14-deoxy-11,12didehydroandrographolide 2. 0 2. 18 2. 14 2. 07 2. 05 4.4 ... and anti-atherosclerotic; neoandrographolide is antiinflammatory, anti-infective and anti-hepatotoxic; 14deoxyandrographolide is immunomodulatory and antiatherosclerotic Among the less abundant...
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Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... extract 32 32 2 .2. 2.1 Anticoagulation activity assay 33 2. 2 .2. 2 In vitro MTT cell viability assay 34 2. 2 .2. 3 Evaluation of antioxidant activities of fractions to 36 2. 2 .2. 3a Determination of free ... 20 06 was between 25 0 to 350 in 100 ,000 Specifically, the age-adjusted annual incidence rate in the urban and rural community was 105 in 100 ,000 and 26 2 in 100 ,000 respectively (Banerjee et al. , 20 06) ... no further clinical study on this compound (Marshall et al. , 20 01; Zhao et al. , 20 01; Lees et al. , 20 03; Green et al. , 20 05; Koziol et al. , 20 06; Lyden et al. , 20 07) Anti-inflammatory The early...
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isolation, selection and identification bacillus subtilis from muddreg of beer
... 2. Material and Method 2. 1.Material 2. 2 .Method .4 2. 2.1.Isolation Method 2. 2 .2. Observation shape and movement of bacteria 2. 2.3 .Measured bacteria size 2. 2.4.Gram ... bacterial specimen on the slide Let the slide stand for approximately 10 to 60 seconds depending on the thickness of the smear on the slide Rinse the crystal violet stain off of the slide with ... strains without bubbling, that’s mean no catalase activity, accounting for 25 % Reaction 2H2O2 catalase 2H2O + O2 Bacillus subtilis are capable of producing enzyme catalase When exposed to H2O2 is...
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Purification and cloning of PCR products
... single-strand ends that are complementary to singlestrand ends of the vector The treated PCR fragment is then added to the vector and annealing is allowed to proceed at 22 °C for before transformation of ... 5’ 5’ANNNNNN 3’ 3’ANNNNNN 5’ Sequence of end ’ Strand invasion of the added sequence by the complementary vector tail and TOPO ligation The other end of the product is joined to the vector by ... pipetting of the solution against the membrane It can sometimes be convenient to remove some of the buffer from the dialysis bag before dislodging the DNA to allow a more concentrated solution to...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... & Pfeifer GP (20 03) Human thymine DNA glycosylase (TDG) and methyl- Active chromatin and DNase I hypersensitive sites 21 8 21 9 22 0 22 1 22 2 22 3 22 4 22 5 22 6 22 7 22 8 22 9 23 0 23 1 CpG -binding protein ... Disruption of the typical chromatin structure in a 25 00 base-pair region at the 5¢ end of the actively transcribed ovalbumin gene EMBO J 1, 22 3 23 0 FEBS Journal 27 8 (20 11) 21 82 22 10 ª 20 11 The Author ... 4503–45 12 FEBS Journal 27 8 (20 11) 21 82 22 10 ª 20 11 The Author Journal compilation ª 20 11 FEBS P N Cockerill 20 6 Ansari KI, Mishra BP & Mandal SS (20 08) Human CpG binding protein interacts with MLL1,...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... 118 829 18861 99.8 (99 .2) 14.7 (5.7) 8.4 (22 .7) 20 80 71 29 9 16.7 20 .4 0.005 (NH4)2SO4 Cocrystallization Chitohexaose Chitotetraose 29 3 – P2 121 21 51.75, 57.60, 82. 51 28 .8–1. 92 1.95–1. 92 138441 1 9106 ... hevamine variants are shown in Table No enzyme activity was detectable for the Asp 125 Ala/Glu 127 Ala and Asp 125 Ala/Tyr183Phe double mutants, and the Asp 125 Ala/Glu 127 Ala/Tyr183Phe triple mutant The single ... Comparison of the Asp 125 Ala/Glu 127 Ala and Asp 125 Ala/Tyr183Phe double mutants with bound chitotetraose (Table 2) with wild-type hevamine complexed with chitotetraose [14] showed that the overall structures...
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Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf
... -3 10 10 10 -5 target 10 rd mode 10 -1 10 10 10 -5 target -1 10 10 10 -5 10 target -1 10 10 10 10 10 target -5 10 rd Frequency (Hz) 10 mode 10 10 10 target mode -1 -1 10 10 10 10 10 -1 10 Frequency ... λ5 -1 10 -1 -2 10 10 -3 10 -4 10 -5 10 -1 10 10 10 -1 -2 -2 -3 10 -4 -5 10 λ4 λ5 10 10 λ3 λ5 10 10 Spectral eigenvalues 10 -1 10 Frequency (Hz) 10 10 10 10 -3 -4 10 -1 10 Frequency (Hz) 10 10 Frequency ... Coordinate 10 -10 Time (s) 10 10 10 5 0 0 0 -10 -10 -5 -5 -5 10 Coordinate -5 -20 10 Time (s) 10 -20 Time (s) -10 10 10 Time (s) 10 -10 Time (s) -10 10 10 Time (s) 10 -1 10 -2 10 PSD PSD 10 10 10 10 10...
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Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... (aa 115– 122 ) of the L3L protein Method 5: MALDI-TOF/TOF MS Direct trypsin digests of the membrane- and core-enriched fractions were also analyzed using MALDI-TOF/TOF MS to take advantage of complementary ... 1 ,2, 3,4,5 39 16 62 4 26 17 2 3 15 26 20 11 2 4 9 10 11 20 10 31 14 34 25 71.6 49.1 29 .9 12. 7 64.3 14.6 91.4 61.1 60 .2 12. 2 32. 0 30.9 70.0 8 .2 58.4 21 .6 23 .2 51.1 12. 6 12. 4 10. 4 19.9 22 .7 63.0 50.6 ... from the G3L protein using Method (MALDI-TOF/TOF) Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) The membraneenriched fraction of the virion was subjected to...
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báo cáo hóa học:"Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" docx
... (aa 115– 122 ) of the L3L protein Method 5: MALDI-TOF/TOF MS Direct trypsin digests of the membrane- and core-enriched fractions were also analyzed using MALDI-TOF/TOF MS to take advantage of complementary ... 1 ,2, 3,4,5 39 16 62 4 26 17 2 3 15 26 20 11 2 4 9 10 11 20 10 31 14 34 25 71.6 49.1 29 .9 12. 7 64.3 14.6 91.4 61.1 60 .2 12. 2 32. 0 30.9 70.0 8 .2 58.4 21 .6 23 .2 51.1 12. 6 12. 4 10. 4 19.9 22 .7 63.0 50.6 ... from the G3L protein using Method (MALDI-TOF/TOF) Mass analysis of a distinct peptide from the G3L protein using Method (MALDI-TOF/TOF) The membraneenriched fraction of the virion was subjected to...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... analysis of Pool using MUC16 specific VK8 and OC 125 antibodies No bands for Muc16 were detected in these analyses The widely employed clinical serum CA 125 assay was also unable to detect CA 125 in ... murine Muc16 Methods Cells, antibodies, and other reagents The anti-MUC16 antibody VK8 [ 12] was a kind gift from Beatrice Yin (Memorial Sloan Kettering, New York, USA) The panel of anti-MUC16 mouse ... absorbance at 28 0 nm The bound glycoproteins were eluted by washing with a step gradient (100 , 25 0, and 500 mM) of α-methylmannopyranoside (α-Me-Man) Fractions from eluted peaks were pooled, and the proteins...
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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... obtained, revealing the structure determinants of their catalytic mechanisms (Tang et al 20 02 and Wang et al 20 05) The analysis showed that the structure of component B resembled that of the trypsyin-like ... enzymes by recombinant DNA technology has been conducted in Escherichia coli (Cho et al 20 04 and Xu et al 20 10) and Pichia pastoris (Ge et al 20 05 and Sugimoto et al 20 05) The recombinant enzymes expressed ... results were reported for Korean and Japanese L rubellus (Cho et al 20 04 and Mihara et al 20 04) FIII-1 and FIII -2 were the two most stable proteases towards a wide range of pH values in comparison to...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... ylevitarapmoc fo snietorpocylg lavral detalosi yltneserp ehT ytilaitnetop evitcetorp rieht rof detset dna mrof erup ni detalosi erew ]7[ aDk 08-57 dna ,]51[ aDk 36 ,] 02[ aDk 68 fo sthgiew ralucelom htiw ... lamron tsniaga snietorp lavral elohw ;5 enaL ,snietorp kcit elohw-itna tibbar tsniaga sPLG lavral ;4 enaL ,snietorp lavral elohw-itna tibbar tsniaga sPLG lavral ;3 enaL ,snietorp tug-itna tibbar ... esorahpeS -AnoC gnisu snietorpocylg lavral fo noitacifiruP snietorp lavral latot eht fo %8. 02 dnuora stneserper hcihw ,gm 5 .2 saw snegonummi dnuob lavral fo dleiy latot ehT )2 giF( snegonummi dnuob...
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Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps
... Additionalset.S9,withcoefficientsAdditional top2,filein thepairscandiB; January fileand integrated with disease methods, for supporting their obesity 12 11 10 and pairs the the association with the the ... Materials and methods) Then, all test cases are pooled together, and the overall performance is evaluated by calculating the fraction of tested disease genes that are ranked above various rank cutoffs ... provided in Additional data file Detailed analysis of the subset of the top 100 ranked obesity genes that does not overlap with the ObesHancock set reveals additional genes with potential roles in obesity...
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báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot
... ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, the Phage ZT -2 and synthetic peptide ZT -2 were shown to specifically bind to the tumor cell surfaces of A498 ... Immunofluorescence Microscopy and Image Analysis Immunofluorescence microscopy was used to study the affinity of synthetic peptide (ZT -2) binding to A498 and renal carcinoma A498 and HK -2 were ... ZT -2 was the best candidate phage clone with the highest specificity Second, immunocytochemical and immunohistochemical staining were performed to confirm the selectivity of the phage ZT -2 to...
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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... 8.0) Then, the membranes were washed and incubated with rabbit anti-tgK polyclonal antibody while using the preimmune serum of normal rabbit as negative control The membranes were then washed and ... characterization of the DNA of Anatid herpesvirus Intervirology 1993, 36:99-1 12 Zhang et al Virology Journal 20 10, 7 :24 1 http://www.virologyj.com/content/7/1 /24 1 10 11 12 13 14 Page of Sandhu , Metwally ... induced BL21 bacteria within pET-32b(+) plasmid; Lane and Lane respectively represented the uninduced and induced BL21 bacteria within pET-32b(+)/tgK plasmid; Lane was the recombinant tgK protein...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25