far you ve learned about why the asp net mvc framework exists and have gained understanding of its architecture and underlyi
Pro ASP.NET MVC Framework phần 10 ppsx
... web development framework, it inherits much of its power from the underlying ASP NET platform, and that in turn from the. NET Framework itself (Figure 15-1) Figure 15-1 ASP NET MVC builds on more ... 15 I ASP. NET PLATFORM FEATURES The framework loads the logged-in visitor’s profile data the first time you try to access one of its values, and saves any changes at the end of the request You ... SQL Server Express edition and not in any other edition of SQL Server—then you can leave these settings as they are However, if you intend to use a non-Express edition of SQL Server, you must...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... semipreparative column with an acetonitrile gradient of 10–35% The purity of the peptides was verified by a reversed-phase analytical HPLC column and the identity of the final products was verified by ... Escherichia coli The synthetic genes were amplified by PCR from six pairs of overlapping synthetic primers containing the two restriction sites of NcoI and BamHI for the SH2 domain and the two restriction ... dependent on the properties of the ligand, [L]T is the total ligand concentration, [P] is the concentration of free SH2 protein, and Kd is the equilibrium dissociation constant Average Kd values...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... to the value of 0.094 for the CRP(cAMP)2 complex, and no substantial changes in the shape of the emission spectra of the donor have been observed, this justies the lack of the alteration of the ... curve shows the intensity decay of the donor alone (D), and the grey dotted curve shows the intensity decay of the donor in the presence of the acceptor (DA) The black solid lines and weighted ... of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor at the excitation wavelength QRF is the quantum yield of the reference...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
... could overcome the positive enthalpy change generated by desolvation of the phosphate group Nevertheless, although the binding enthalpy of the inhibitors is negative, the binding enthalpy of 6PG ... carboxylate group of 6PG; nevertheless, the Ki value is far below the Kd value of the substrate The very tight binding of 4PEX can be explained by suggesting that the planar nature of the hydroxamate group ... involves only the coenzyme, or both NADP and substrate, 6PGDH was titrated with 6PG and 4PE in the presence of saturating concentrations of aPyADP and NADP, respectively The titration of the enzyme–aPyADP...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... whereas the maximum of the less quenchable component remains unchanged at 338 nm The maxima of the resolved spectra and their relative intensities, measured as the areas under each of the resolved ... curve The plots of the inverse time constant (kobs) are linear (Fig 9) and the values of koff and the association-rate parameter, kon, listed in Table 3, were determined as the intercept and the ... between the two CRP domains and subunits involves the Asp1 38 residue, located in the CRP hinge region, which represents part of the signal transduction network Depending on the location of the CRP-binding...
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Báo cáo khoa học: Modulation of heme and myristate binding to human serum albumin by anti-HIV drugs An optical and NMR spectroscopic study potx
... observed in the absence of any drug and in the presence of abacavir In the absence of myristate and at a myristate concentration of 1.0 · 10)5 m, curves cannot be fitted using Eqn (1): however, ... cooperation is observed in the absence of myristate: therefore, the Kd value cannot be obtained using Eqn (1) In the presence of myristate the curves assume a hyperbolic behaviour and the values reported ... conformation and substitution [35,38–42] In HIV-infected individuals the primary target of therapy is the HIV itself, but most of the clinical manifestations are related to the effect of HIV on the immune...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... PCSK9-S127R, only the upper and lower, respectively, halves of the ranges are shown, to avoid overlap of the error bars The results are expressed as percentage of control (c.p.m in the absence of unlabeled ... higher-affinity and lower-affinity binding sites are listed in Table The high-affinity and the low-affinity sites accounted for 25% and 75% of the total binding, respectively These estimates of the relative ... 42% and 22% of dissociation occurred in the rapid phase after short and long incubation times, respectively The dependence of the kinetics of [125I]TCPCSK9-D374Y dissociation on the length of...
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Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... S3, the signal was abolished to a large extent and extraction of any meaningful binding parameters was difficult Such problems are not unusual and have been observed in the case of several other ... calcium displaces the dye bound to calcium-binding sites of the protein Other proteins of this superfamily, namely c-crystallin [20] and AIM1-g1 [15] also induce the J band of the dye indicating ... structures of bB2-, bB1- and c-crystallins have been solved, calcium ion was not noticed in their solved structures [6,41,42] This could be due to several technical reasons However, the most probable...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... 8, the linewidth change is significant (about 20 Hz) for the protein in the N state, and becomes larger (about 40 Hz) in the presence of saturating concentration of myristate On the other hand, ... Paramagnetic contributions to the 17O-NMR linewidth (DW) were obtained by subtracting the width of the H217 O signal in the presence of HSA from the width of the H217 O signal in the presence of ... on the conformational state of HSA and on the occupancy of the myristate binding sites The relaxation rate change is highest between pH 5.5 and 8.0, where HSA is in the native form (N state) The...
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Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx
... the maximal binding of CD11a/CD18 and CD11b/ CD18 to ICAM-4 [14,28] Here we have investigated the effect of divalent cations on the binding of ICAM-4 transfectants to the I domains of CD11a and ... transfectants to the I domains was obtained with the ICAM-1 mAb LB-2 reacting with the first domain of ICAM-1 and the mAbs GP8914 and GP8923 which have been mapped to domains and of the ICAM-1 molecule ... Biochem 270) The most efficient binding of all the ICAM L cell transfectants was observed in the presence of Mn2+ (Fig 8) In the absence of cations, the binding of ICAM-1 transfectants to the CD11a...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... from the 1D spectrum 25% relative intensity in the STD spectrum Obviously, Pro1 and Asp2 get more saturation from the protein than the remaining residues of the ligand and therefore have more and ... can be seen from the three panels in Fig 4, the initial slopes of the build-up curves of the geminal proton pairs are very different The build-up rate of the b protons of Asp2 is about 2.2-fold ... contact to the protein In the 3D structure of PDTRP docked into the binding site of SM3 Pro1 and Asp2 fill a deep cavity of the antibody while Arg4 and Pro5 have less contact to the surface of SM3...
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
... [20]: koff ẳ koff2ị ẵibuprofen=K2 ẵibuprofenị ỵ koff3ị ẵibuprofen=K3 ẵibuprofenịị ỵ kỵ off 7ị where koff(2) and koff(3) indicate values of koff occurring at K2 < [ibuprofen] < K3, and at ... K3 < [ibuprofen], respectively, and kỵ is the koff value obtained in the off absence of ibuprofen Data analysis Kinetic and thermodynamic data were analyzed with the matlab program (The Math Works, ... However, the absence of domain III in tHSAheme precludes the binding of ibuprofen to its primary cleft [1921] (Table 1) The analysis of thermodynamic parameters reported in Table allows the following...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... [32] Levels of TfR2 are highest in hepatocytes, the predominant site of HFE expression, and recent studies have confirmed that the two proteins are capable of interacting [33,34] The nature of these ... groove that mediates antigen binding and presentation to CD8 + cytolytic T cells In HFE, the proximity of the two a helices and the presence of amino acid side chains that project into the groove ... 3222 in the presence or absence of tunicamycin Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosylated...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... excess of the same, unlabeled peptide, but also by an excess of the unlabeled peptide from the other binding sites (data not shown) The validity of the results obtained for the binding of CaM to the ... bound CaM in the presence of 25 lm Ca2+ with KD on the order of 100–150 nm These fractions have in common the previously identified CaM-binding domain BD-C2 In the absence of Ca2+ these BD-C2 fragments ... interaction then results in opening of these channels [9,10] The mechanism of CaM-mediated closure of hEAG1 channels is far from understood mainly because of the lack of structural information on the...
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Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot
... 47 and 23%, respectively Because of differences in the relative orientation of CH1 and CH2 subdomains in the structures of utrophin, dystrophin and fimbrin, not the whole ABD, but the CH1 and ... 3.25 A On the other hand, only 59% of the corresponding contact areas of pleABD/2a and utrophin are hydrophobic and there are ve and six hydrogen bonds, respectively Moreover, the secondary structure ... plectin and lowest with fimbrin (Table 3) The nearly identical conformations of the pleABD/2a structure and the corresponding subdomains of the A and B molecules of utrophin and dystrophin probably have...
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Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoflurane to a mammalian b-barrel protein doc
... This software provides the best-fit values for the heat of binding (DH°), the stoichiometry of binding (n), and the association constant (Ka) from plots of the heat evolved per mol of anesthetic ... function of the maximum possible quenching (Qmax) at an infinite halothane concentration ([Halothane]) and the affinity of the anesthetic for its binding site (Kd) in the vicinity of the tryptophan ... Curve a (s) is the control rate of hydrogen exchange in the absence of anesthetic The y-axis is the molar ratio of unexchanged hydrogen to protein The most rapid time point acheivable in these...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... concentration of the proteinligand complex, and [P] and [L] are the concentrations of the free protein and free ligand (i.e inhibitor), respectively [68] Expressed in terms of r, dened as the number of ... lm) overnight and analyzed as previously The same relative abundances of the free and bound enzymes were observed with either 10 or the extended incubation periods within the error level of the ... observed when comparing the CTXS-Xyl5-Me complex with the native structure of CTX The space group and cell dimensions in the complex structure were the same as for the native protein, and the...
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Data Mining Classification: Basic Concepts, Decision Trees, and Model Evaluation Lecture Notes for Chapter 4 Introduction to Data Mining pptx
... Given a collection of records (training set ) – Each record contains a set of attributes, one of the attributes is the class Find a model for class attribute as a function of the values of other ... test set is used to determine the accuracy of the model Usually, the given data set is divided into training and test sets, with training set used to build the model and test set used to validate ... ni is number of records in partition i – Measures Reduction in Entropy achieved because of the split Choose the split that achieves most reduction (maximizes GAIN) – Used in ID3 and C4.5 – Disadvantage:...
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Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx
... demonstrating the antagonism of the two effectors When the ratio of GTP to UTP was varied over the same range, the effects were similar to the effects of GMP and UMP; the values for the apparent ... concentrations of the nucleotides Measurements of the binding of RNA to PyrR over a wide range of nucleotide concentrations in the filter binding assay yielded the concentration at which the effect of the ... below The masses calculated from the sequences of the native and His-tagged PyrR in the tetrameric forms are 79.8 and 91.2 kDa, respectively Since these weight average masses are calculated from the...
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Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc
... 2152 The main drawback in gene therapy has been the targeting and successful delivery of suicide genes into the cells of interest When this obstacle is overcome, we will have an arsenal of very ... the active site The interactions of the deoxyribose and the base are identical to those of the dT complex, except for the fluoride atom replacing the methyl group on the base (Fig 2A) In the dC ... Surprisingly, there were two sulfate ions present – one bound in the P loop, as observed in the other substrate complexes, and the other located between the first sulfate ion and the substrate There Nucleoside...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu