... by the amount of internalized agonist[20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Roleof helix 8 and C-termini in bradykinin ... 2004 FEBS The roleof helix 8 andofthe cytosolic C-termini in the internalization and signal transduction of B1 and B2bradykinin receptorsAlexander Faussner1, Alexandra Bauer1, Irina Kalatskaya1, ... addition of scintillation fluid. Nonspecific bindingwas determined inthe presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe-cific binding.Internalization of...
... Sentence stress organizes the words inthe sentence into logically connected thought groups by joining the unstressed syllables to the main stressed syllable inthe group and marking the end ofthe ... lose their stress. The most important words in the sentence receive stronger stress. The last stressed word inthe sentence receives the strongest stress with the help of falling or rising intonation. ... distinguish between four levels of stress: major sentence stress, major word stress, minor word stressand unstressed. Thus, inthe sentence: “I walked home inthe rainstorm”. ‗I‘, in , and...
... resultfrom the modifications inthe interactions between the catalytic glutamates andthe residues R97 and Y331,indicating that these residues play a roleinthe modulation of the Sfbgly50 pH optimum.Sequence ... mMBicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and thendetermining the activity remaining at the optimum ... [10,12].Nevertheless, it is not possible to assume that the same isoccurring inthe mutant R97M, because there is no data on the interactions between the substrate andthe arginineresidue.Finally, another...
... unsure ofthe mechanism of DNA-induced stabilization ofthe protein, and whe-ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... understood, and we have investigated thesequestions in this work. We sought to determine the roleof protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea,GdnHCl ... against all the forms of denatura-tion we tested. This is consistent with the observation of increased stability ofthe single-chain version of the wild-type dimer [30] and is reminiscent of the...
... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone.Materials and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
... Li4AbstractIntroduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
... up to 0.6% and then decreases due to the formation ofthe Cu-agglomerates, suggesting that a crucial roleofthe metallicagglomerates was involved inthe processes. The PSSHGfor the crystal ... respectively, A is the area ofthe pumping beam which processes Gaussian-likeform, n(x) and n(2x) are, respectively, the refractiveindices for the pumping and PSSHG doubled frequencies,vijkare the components ... large relaxation timeobserved inthe PSSHG pulses demonstrates the principal role of long-lived electron–phonon states inthe observedeffects explained within a model of photostimulated elec-tron–phonon...
... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that do not harm the viability or activity of DCs.Acknowledgments ... cells, producing major cytokines involved in immune responses. Importantly, taxol increased the expression ofthe anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr The roleof Bcl-xL and nuclear factor-κB inthe effect of taxol on the viability of dendritic cellsMi-Hyoung Kim1, Hong-Gu Joo1,2,*1Laboratory of Veterinary Pharmacology, College of...
... importantly, the unfavourableeffects of an excess of water on the soilstructure and on the rooting of the treesowing to the impermeability of the subsoil. Of the recent ... growth increase in the ’young’ rings (< 60 years) of sessile oak was + 81%, and that of young rings of pedunculate oak was + 49%. The corresponding increase in the ... on the wood quality of the butt log), onefrom the northern side of the trunk and the otherfrom the southern side. Throughout the text, agerefers to that determined...
... only criticalfor the stability ofthe dimer, it may also be involved in maintaining the integrity ofthe active site. These resultsclearly suggest that the dimer interface inthe Y74W*mutant ... bond with the backbone CO of F102. Fur-thermore, the orientation ofthe side chain ofthe tworesidues brings the guanidinium plane andthe aro-matic ring of F102 into close proximity, with ... elevated temperature and in the presence of denaturants [27].Extending these studies, we examine here the effect of increasing the bulk ofthe residue at position 74.Surprisingly, the Y74W mutant...