the role of oxidative stress and inflammation in dry eye disease

... result from the modifications in the interactions between the catalytic glutamates and the residues R97 and Y331, indicating that these residues play a role in the modulation of the Sfbgly50 pH optimum. Sequence ... m M Bicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and then determining the activity remaining at the optimum ... [10,12]. Nevertheless, it is not possible to assume that the same is occurring in the mutant R97M, because there is no data on the interactions between the substrate and the arginine residue. Finally, another...

Ngày tải lên: 23/03/2014, 15:21

... resistance to the action of aerosols is the result of the strength of the structures protecting the leaf, and increases with sclerophyllia and with the thickness of the cuticle. ACKNOWLEDGEMENTS The ... sam- ples and deposits When interpreting the results of the chemi- cal analysis of rainwater and deposits it is necessary to bear in mind the interactions between the matrix and ... near the coast and 2-3 km inland. Samples of bulk deposits were taken from pine trees (Pinus pinea L) from the section of the crown exposed to the sea and also from the...

Ngày tải lên: 08/08/2014, 19:21

Ngày tải lên: 11/08/2014, 05:22

Ngày tải lên: 12/08/2014, 13:22

... oxida- tive stress. The observed increase of reducing species in urine would mirror an increased level in blood originat- ing from a response to oxidative stress in the body mon- itored by the urinary ... only 10% of urate is excreted in the urine (the remaining 90% being recirculated by the renal system [3]), an increase of this antioxidant in blood will also lead to an increase in urine. The fact ... H, Savant Instruments Inc.). The final volume was deter- mined by gravimetry, assuming that the entire methanol was removed during the conc entration in the SpeedVac and that the d ensity of the remaining...

Ngày tải lên: 20/06/2014, 00:20

... by the amount of internalized agonist [20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Role of helix 8 and C-termini in bradykinin ... FEBS The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B 1 and B 2 bradykinin receptors Alexander Faussner 1 , Alexandra Bauer 1 , Irina Kalatskaya 1 , ... addition of scintillation fluid. Nonspecific binding was determined in the presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe- cific binding. Internalization of...

Ngày tải lên: 07/03/2014, 16:20

... of GSK3 (using Li or SB-415286) appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant ... increases in glucose uptake and glycogen synthesis allowing for more effective ÔchannellingÕ of glucose into glycogen in response to insulin. However, one of the potential difficulties in interpreting ... phosphatidylinositol 3-kinase, p70, S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammalian target of rapamycin (mTOR) pathway in the L -leucine-induced up-regulation...

Ngày tải lên: 08/03/2014, 08:20

... Sentence stress organizes the words in the sentence into logically connected thought groups by joining the unstressed syllables to the main stressed syllable in the group and marking the end of the ... lose their stress. The most important words in the sentence receive stronger stress. The last stressed word in the sentence receives the strongest stress with the help of falling or rising intonation. ... distinguish between four levels of stress: major sentence stress, major word stress, minor word stress and unstressed. Thus, in the sentence: “I walked home in the rainstorm”. ‗I‘, in , and...

Ngày tải lên: 17/03/2014, 16:12

... phenylalanine was therefore mutated to Ala, Leu, Trp or Tyr. These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme ... PAA and derivatives thereof, while maintaining the hydrophobicity of the binding site. To test the effect of the mutations on the specificity for phenylacetylated substrates, the steady-state kinetic ... k cat represents the rate of acylation and K m equals the binding constant of the substrate to the free enzyme. The results then indicate Ó FEBS 2002 Role of active-site phenylalanines in penicillin acylase...

Ngày tải lên: 17/03/2014, 23:20

... discoideum to oxidative stress, and we now show the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine (HA) to induce in situ generation of H 2 O 2 and ... development. HA-induced oxidative stress activates PARP within 5 min (Fig. 4A,B), and its role during oxidative stress is further confirmed by the use of low concentrations of benzamide. Preincubation of cells ... H 2 O 2 and monitored the effect of benzamide, a PARP inhibitor, on oxidative stress- induced changes in D. discoideum development. Interestingly, oxidative stress resulted in PARP activation within...

Ngày tải lên: 23/03/2014, 07:20

... unsure of the mechanism of DNA-induced stabilization of the protein, and whe- ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... understood, and we have investigated these questions in this work. We sought to determine the role of protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea, GdnHCl ... against all the forms of denatura- tion we tested. This is consistent with the observation of increased stability of the single-chain version of the wild-type dimer [30] and is reminiscent of the...

Ngày tải lên: 30/03/2014, 11:20

... acquisition, analysis and interpretation of data and drafting of the manuscript. EH was involved in design, interpretation of data, drafting of the manuscript and supervision. ØE was involved in conception and ... design, interpretation of data, drafting of the manuscript and supervision. BL was involved in analysis and interpretation of data. AMB is the guarantor for this paper. Acknowledgements The study ... districts and rural ('lensman') districts. The two categories have the same education and training, but in the rural districts they work in smaller communities, often including large...

Ngày tải lên: 20/06/2014, 00:20

... visualized the mineralizing cells with 2% Alizarin Red. After rinsing in distilled water and air drying the samples, we mounted the cov- erslips on microscope slides using Fluoromount and examined and ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone. Materials and ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing...

Ngày tải lên: 20/06/2014, 04:20

... visualized the mineralizing cells with 2% Alizarin Red. After rinsing in distilled water and air drying the samples, we mounted the cov- erslips on microscope slides using Fluoromount and examined and ... Li 4 Abstract Introduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing...

Ngày tải lên: 20/06/2014, 07:20

... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that do not harm the viability or activity of DCs. Acknowledgments ... cells, producing major cytokines involved in immune responses. Importantly, taxol increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr The role of Bcl-xL and nuclear factor- κ B in the effect of taxol on the viability of dendritic cells Mi-Hyoung Kim 1 , Hong-Gu Joo 1,2, * 1 Laboratory of Veterinary Pharmacology, College of...

Ngày tải lên: 07/08/2014, 23:22

... damage. Further significant decreases in GSH levels in our study may be due to either the inhibition of GSH synthesis or increased utilization of GSH for detoxification of toxicant induced free ... However, an increase in catalase activity enhances the scavenging ability of erythrocytes to handle the hydrogen peroxide to molecular oxygen and water [11,29]. GSH-Pxs catalyze the peroxides and reduce ... significantly reduced (p ＜ 0.05) in all groups compared to the control group. Similar finding have been reported in other study during oxidative stress [24]. No significant changes in GST activity was...

Ngày tải lên: 07/08/2014, 23:22

... characteristics of the Palestinian political and eco- nomic situation may have contributed to these findings indicating the significant relation of QOL and income and education in Gaza Strip. Unemployment and ... life in these patients. Background Quality of life (QOL) is a phrase often used in health care settings at policy and administration levels, in clinical assessments of therapies, and in clinical ... the dis- ease in the previous year, on the type of brain lesion of stroke or the severity of the CVD condition potentially influencing QOL were obtained. However, for the pur- pose of this study...

Ngày tải lên: 08/08/2014, 21:20

Ngày tải lên: 09/08/2014, 02:20

Ngày tải lên: 09/08/2014, 08:23

Ngày tải lên: 10/08/2014, 10:20