effect of high fat diet versus normal fat diet in mice fat and muscle
Báo cáo y học: " The innate immune repertoire in Cnidaria - ancestral complexity and stochastic gene loss" pptx
... Summary of domain structures of TIR domain-containing proteins identified in selected Cnidaria Summary of domain structures of TIR domain-containing proteins identified in selected Cnidaria nomic) and ... versus Acropora) are unlikely to be informative in terms of understanding the origins of genes [50] - loss of a gene from Nematostella and loss of a specific function in the indirect developing ... characteristic domain structure consisting of an extracellular amino-terminal domain containing leucine-rich repeats (LRRs) responsible for pattern recognition and an intracellular Toll interleukin receptor...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... stranded sequence was determined for a region of this construct containing galA and some of the flanking regions, resulting in the genomic sequence of A niger galA The presence of one putative intron ... and 0.3% L-arabinose and is predominantly linear Potato arabinogalactan consists of 86% D-galactose and 6.6% L-arabinose, while soy arabinogalactan consists of 57% D-galactose and 38% L-arabinose ... by introducing an NsiI site at the translation start point of galA and by then cloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx
... aggregation of denatured insulin B-chain induced by the addition of 20 mM dithiothreitol in NaCl/Pi buffer Curve 1, insulin alone; curve 2, insulin + Bt-Lon (50 lg); curve 3, insulin + Bt-Lon ... 4, insulin + Bt-Lon (100 lg) The protein concentration of insulin was 300 lgÆmL)1 in NaCl/Pi buffer (pH 7.4) The ratios (w/w) of insulin to Bt-Lon are given in the inset used as a measure of protein ... and are considered to be involved in protein–protein or protein–DNA interaction [36] Analysis of promoter and heat-induced transcription of Bt-lon The lon gene of E coli and B subtilis belongs...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... The pI of NaoA was determined from a standard curve of pI and migration distance (cm) of protein standards Enzyme assay and analytical methods E coli BL21(DE3) containing plasmid pNA101 was grown ... was determined by detecting the formation of nitrite using the method of Ida et al [31] Km and Vmax of purified NaoA were determined from a double-reciprocal plot according to the LineweaverBurk ... fragments of m/z 58 (M+) and m/z 43 consistent with those of acetone standard Properties of NaoA The pI of NaoA is about 5.2 according to the standard plot between the protein’s pI and its migration...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... minima at 207 nm and by a maximum at 190 nm The negative band at 207 nm had a lower intensity in the case of rBmKIM in comparison with that in the spectra of AaHIT2 (a-toxin) and CssII (b-toxin) ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 37 Loret, E.P., Mansuelle, P & Rochat, H (1990) Neurotoxins Active on insects: amine ... jugular vein was cannulated for drug administration The lead II ECG maintained continuous readings using a polygraph system One hour after the intravenous administration of BmKIM (dissolved in distilled...
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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx
... years it was reported that the accumulation of point mutations in VP4 and VP7 genes was the main cause of the failure of the G or P-typing of rotavirus strains [7,8,12,16-21] At least, there are four ... [7,12,17,20,21], and In order to evaluate how many G1 strains included in the GenBank database have these four specific mismatches and its clustering within specific lineages, we collected 173 sequences of ... lineages are represented in the tree as follows: lineage I (orange), lineage II (green), lineage III (blue) and lineage IV (red) lected to understand this complex epidemiology [15] However, in...
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Báo cáo y học: "Lack of association between mannose-binding lectin gene polymorphisms and juvenile idiopathic arthritis in a Han population from the Hubei province of China" docx
... The role of mannose-binding lectin in health and disease Mol Immunol 2003, 40:423-429 Summerfield JA, Ryder S, Sumiya M, Thursz M, Gorchein A, Monteil MA, Turner MW: Mannose binding protein gene ... those of healthy controls from Hong Kong and Europe [6,7] The mutation type of codon 54 was seen in 25.0% of Chinese children (n = 48), 21.4% of adults from Hong Kong (n = 196) and 24.6% of European ... as follows: an initial denaturation at 94°C for minutes; 34 cycles of denaturation at 94°C for 30 seconds, annealing at 60°C for minute and extension at 72°C for minute; and a final elongation...
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Báo cáo y học: " Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand" ppsx
... Moreover, some amino During 2005-2009, 12 swine influenza viruses were isolated from areas of intensive swine farming in central and eastern regions of Thailand The 12 swine influenza isolates ... divided into distinct lineages, Eurasian swine lineage and classical swine lineage Based on topology of the phylogenetic tree, the Eurasian swine lineage is closely related to the avian lineage and ... monitoring influenza A viruses None of the unique amino acids of NP lineages identified in this study is involved in RNA binding activities [10] They are mainly correlated with host specificity of...
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Báo cáo khoa học: " Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005" ppsx
... Depending on virus strain NS1 consists of 124–237 amino acids in length and is expressed exclusively in infected cells The NS1 protein contains two functional domains: the Nterminal RNA-binding ... important to prevent interferon mediated antiviral responses Binding of NS1 protein to both single- and double-stranded RNA might: (a) inhibit activation of interferon induced protein kinase PKR [7], ... Obenauer and colleagues (2006) that NS1 have a PDZ binding motif at the very end of the protein PDZ domains are protein-interacting domains present once or multiple times within certain proteins and...
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Báo cáo y học: "Nef gene evolution from a single transmitted strain in acute SIV infection" ppt
... modeling [1-3] Major goals of such analyses include the characterization of the transmitted strains, estimating the timing of infection based on the level of sequence diversity, and distinguishing ... extent of virus replication during the acute phase of infection and the subsequent virus set point and disease course [11] as reported in HIV1 infections [12-14] Genetic evolution during SIV infection ... Nucleotide and amino acid base substitutions within weeks post SIV infection Nucleotide and amino acid base substitutions within weeks post SIV infection Longitudinal nucleotide (A) and amino acid...
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Báo cáo y học: " A method to estimate the efficiency of gene expression from an integrated retroviral vector" potx
... (FCS) (Gibco) and 5% penicillin-streptomycin (Gibco) Jurkat cells [14] were maintained in RPMI1640 medium (Gibco) also containing 10% FCS and 5% penicillin-streptomycin Where puromycin selection ... containing higher proportions of puromycin resistant cells giving higher cell counts at an earlier time point An equal number of cells from the sample were challenged with puromycin (curve in red) ... in the presence of puromycin, only cells harbouring an active provirus would be puromycin resistant and able to survive and proliferate A family of cultures containing different proportions of...
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Tài liệu Retrieving a Single Value from a Query pdf
... retrieving a single value using an output parameter or using a DataReader, it allows a single value to be returned with the least code and may therefore improve readability and maintainability ... ExecuteScalar( ) method of the Command object returns a single value from the data source rather than a table or data stream While the ExecuteScalar( ) method does not result in a performance improvement ... result, the first column of the first row is returned as a scalar value A null reference is returned if the result set is empty or if the result set is a Ref Cursor when using the Oracle NET data...
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Tài liệu Pass a Dataset Back from an XML Web Service docx
... btnLogin Add the code in Listing 13.6 to the Click event of btnLogin This code once again checks the username and password by calling the TestUserMethod of the Web Service If the username and password ... Open the Windows Project where you created the Login windows Form Create a new Windows Form and place the controls shown in Table 13.2 in the order displayed in Figure 13.14 Table ... tables on the receiving end, as displayed in step of this How-To, you have to refer to tables in the dataset by their ordinal values Therefore, some information in the description of the Web Service...
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Tài liệu Getting a Sequence Value from Oracle pdf
... variables, and constants using System; using System.Configuration; using System.Windows.Forms; using System.Data; using System.Data.OracleClient; private const String STOREDPROCEDURENAME = "SP0404_INSERT"; ... ConfigurationSettings.AppSettings["Oracle_ConnectString"]); // Create the command for the insert stored procedure OracleCommand cmd = new OracleCommand( ); cmd.Connection = conn; cmd.CommandText = STOREDPROCEDURENAME; ... setting the return value of a stored procedure The sample demonstrates using the NEXTVAL and CURRVAL keywords to generate the new sequence value when inserting a row using a stored procedure and...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells ... in the presence of DTT Fig 12 showed chitin gel before (N1) and after (N2) releasing MCoTI-II Fig 12 Changing in SDS-PAGE protein pattern of chitin gel before and after releasing ReMCoTI-II 1,2: ... system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin ,and proteinases from Plutela...
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Tài liệu Voices of Fear and Safety¿ Women¿s ambivalence towards breast cancer and breast health: a qualitative study from Jordan pdf
... cancer as an incurable disease associated with suffering and death; b) fear of the risk of diminished femininity and husband’s rejection; c) fear of social stigmatization of the disease, and d) apprehensions ... of the risk of diminished femininity and husband’s rejection In all the FGDs, women associated breast cancer with fear of a distorted body image and loss of femininity because it inflicts a body ... close interaction between individual, family and community influences on Jordanian women’s screening behavior The first theme was seen in the hindering effect of women’s prioritizing children and...
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Breaking into the Game Industry: Advice for a Successful Career from Those Who Have Done It
... longtime friend and love, John Romero, for being a living, walking, breathing bible of gameplay, game design, and coding knowledge, and love and inspiration To have that kind of genius in my world ... greatly how well connected the professors are Some professors are out there in the industry, going to GDC, networking with and gaining respect in the game industry And remember that all schools ... Gamasutra.com in 2008 and one of the 100 most in uential women in the game industry by Next Generation magazine in 2007 Nerve magazine also called her one of the 50 artists, actors, authors, activists, and...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... labeled within the intact protein at its a-amino group and represents the amino terminal amino acid of P40 The efficiency of this reaction was quite high, as judged from the signal intensities of the ... (CAF) [30]: during the first step of the labeling reaction the e-amino groups of lysine are specifically converted to homoarginine In a second step, the free amino terminal groups of the peptides ... determined the N-terminus of P40 as it appears in protein extracts of M pneumoniae Determination of the N-terminus of P40 by Edman degradation failed due to the limited amount of starting material...
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