... using the primers 5¢-TGTAACCTCTACTCCCA CCCA-3¢ (forward) and 5¢-TCTGAGTCCTTATCCAGC CTG-3¢ (reverse) corresponding to a region ofthe c- Jun promoter that expands between the start transcription ... 5¢-GTCGTCCAGATACTCAGCAT-3¢ (reverse) and primers for cyclin D1: 5¢-TGTTCGTGGC CTCTAAGATGA-3¢ (forward) and 5¢-GCTTGACTCCA GAAGGGCTT-3¢ (reverse); primers for 18S rRNA: 5¢-GAGTATGGTCGCAAGGCTGAA-3¢ ... target, the cyclin-dependent kinase inhibitor p21WAF1/CIF1, suggesting that one particular function of c- Jun is the negative regulation ofp53 transcription The fact that the binding of RAR to...
... to introduce the codon for P761 and a stop codon to prevent GFP expression: mutVRL-1_F (5¢-TCAGGTCCTCCAG TCCCCCTAAGGGCAATTCTGCAGAT-3¢) and mutVRL-1_R (5¢-ATCTGCAGAATTGCCCTTAGGGGGA CTGGAGGACCTGA-3¢) ... obtain fragment C with 836 bp; rVRL-1–1618F: 5¢-TTCCTGCTGGTCTACCTG GT-3¢ and rVRL-1–2251R: 5¢-CTTCCTCTGAGGCACT GTTC-3¢ to obtain fragment D with 633 bp All primers were purchased from MWG Biotech ... cells PCR primers were designed according to the rat VRL-1 sequence (Acc No AF129113) rVRL-1–34F: 5¢-CTGGAGACTTCCGATGGAGA-3¢ and rVRL-1–568R: 5¢-CATCCGCTCCATTCTCTACC-3¢ to obtain fragment A with...
... suggesting that PKCε may contribute to the resistance of clear cell RCC cells to cytotoxic drugs Figure Changes of caspase-3 activity in 769P cells after PKCε downregulated and cytotoxic drug treatment ... 5’-ATGGTAGTGTTCAATGGCCTTCT-3’; reverse, 5’-TCAGGGCATCAGGTCTTCAC-3’ The sequences of internal control glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were as follows: forward, 5’-ATGTCGTGGAGTCTA ... and chemosensitivity of RCC Conclusions Our results confirm the role of PKCε as an oncogene in RCC, especially inthe subtype of clear cell, suggesting that PKCε might be a potential treatment target...
... was positive if the ratio between the optical density ofthe specimen andthe mean optical density ofthe control was or more Clinical data The following clinical characteristics were recorded: ... control window and a pink line inthe test window, and negative if there was a blue line inthe control window and no pink line inthe test window Serological study Two ofthe 12 centers also tested ... to the sample port ofthe test cassette The test was read after incubation for exactly minutes at ambient room temperature Tests were recorded as positive if there was a blue line inthe control...
... Harmonisation Conferences, where the coast guards and maritime directorates ofthe Arctic littoral states and other interested states also took part.131 As to the Arctic Council, among its objectives is the ... Areas ofthe Far North and Marine Areas Adjacent to the Northern Coast ofthe USSR22 (Environmental Edict) in its Article specified: Inthe marine areas adjacent to the northern coast ofthe USSR ... the ice-covered exclusive economic zones inthe Arctic The aim is to demonstrate how the state practice, including legislation, of relevant Arctic states has been shaping the contours of Article...
... International Financial Institution IFI International Monetary Fund IMF Instituto Nacional de Estadistica (National Statistical Institute) INE Instituto Nacional de Estadistica e Informatica INEI ... set of pre-treatment attributes In this formulation, the program indicator D is assumed to be independent ofthe potential outcomes conditional on the attributes used to select the treatment ... probability of exit The country studies tested for this potential source of bias by re-estimating outcome models dropping the bottom and 10 percent ofthe treatment group that might have failed in the...
... (5¢-CTTGATTTGGATCCTTTAAGGTA TAGC-3¢), ATnI1F and ATnI128R (5¢-GTTCCGGATC CTATCTTCTGGCTTCC-3¢), ATnI130F (5¢-GCCAGAA CCATGGCGGAGGAAC-3¢) and ATnI292R, ATnI130F and ATnI252R (5¢-CAAGTTTGGGATCCTATTTGTTAA CTTTTC-3¢), and ... corresponding interaction in rabbit TnI–TnC inthe absence of divalent cation Therefore, this interaction potentially participates in both the Ca2+-dependent activation ofthe contraction andthe maintenance ... resulting inthe release ofthe inhibition and muscle contraction [17] The structural TnC-binding site interacts with the C- domain of TnC in both the relaxed and contractile states, which plays...
... hazard; rather, the overall hazard ofthe substance will be determined by toxicity thresholds based on direct toxicity to aquatic life On the other hand, if the predicted tissue concentration inthe ... stage to define a speci c UWM and expect that it will stand the test of time Rather, we suggest a general structure of a model inthe full expectation that it will change inthe light of experience ... BACKGROUND The approach of characterizing the potential hazard of organic chemicals by considering those inherent, chemical-speci c properties that relate to their potential persistence, bioaccumulation,...
... transfected with these constructs Whereas cells transfected with the construct lacking the hairpin produced detectable amounts of HA-tagged IFN-b, no translation product was detectable with the construct ... Moreover, the transcriptional blockade by actinomycin D abrogated the increase of mRNA accumulation due to the cycloheximide (Fig 1E) This latter observation indicates that increased accumulation of ... transfected into Hec-1B cells The cells were infected (or not) with the Sendaı¨ virus for h and actinomycin D (5 lgÆmL)1) was added inthe culture medium Total RNA was extracted at the indicated times...
... probably strengthen the liquidity constraint of rms, thus increasing their short-term credit demand Putting all these arguments together, the theoretical sign ofthe parameter of industrial production, ... b and y standing for the loan interest rate, the bond interest rate andthe income elasticities of loan demand respectively, y the real output and p the price of output The positive dependance ... central bank as operating on interest rates rather than controlling the base money The interest rate control assumption reects the actual conduct of monetary policy in Poland since 1994 According...
... charge to their internal and external customers Thus, this price comprises more than the actual or standard costs of a test It includes all costs associated with the carrying out of a test, including ... influencing the distribution Among others these are the properties ofthe substances to be tested, unexpected events during the tests, and intermediate results; because they often determine the effort and ... of tests they conducted inthe past one or two years The critical question certainly concerns the average capacity since this knowledge is needed to determine the number of studies the labs could...
... panic, and this I believe to be true inthe case of other countries Why is this? Is it not because the people instinctively turn to tinkering at and changing their chief tax the tariff whenever they ... note attached to this Introduction, a new tariff or a general change of duties is apt to precipitate a panic, on account ofthe unsettling of business, and that the consequent shaking of credit adds ... products, and to lower the price of all that the farmer has to buy But the farmers vote as the manufacturers and other active classes ofthe minority of our voters may influence; and only twice in...
... differentiation ofthe cells into astrocytes or oligodendrocytes, but are connected with the lack of serum inthe culture medium Furthermore, these data indicate that growth arrest and GFAP expression ... medium Taken together, the data presented here indicate that the P2Y12 receptor acts in favor of cell growth and proliferation because of (a) the reduction in cAMP concentration, (b) the stimulation ... extracellular concentration of ATP should first in uence the P2Y2 receptor, and furthermore its metabolite should in uence the P2Y12 receptor as strongly as P2Y1 We know that the concentration of the...
... varies inversely as the square ofthe distance and directly as the illumination ofthe writing; then the amount of light lost at the greatest obscuration ofthe sun was 2/5ths that ofthe unobscured ... complementary way to that ofthe reflected component as the crystal was rotated Assuming the total intensity to be conserved, he deduced that the reflected component was proportional to the cosine ofthe ... measurement of light and colour from its first hesitant emergence to its gradual construction as a scienti c subject The first traces changing attitudes concerning quantification The mathematization of...
... guides to the character ofthe things that hurt us Particular kinds of smells, on the other hand, are in general characteristic of this or that type of object These considerations I hope constitute ... experience Analytical reection puts forward, instead ofthe absolute existence ofthe object, the thought of an absolute object, and, through trying to dominate the object and think of it from no point ... thought In other words, the beliefs and expectations ofthe perceiver are thought to have no inuence on the character of Introduction the subpersonal computations that constitute perception Thus,...
... ofthe participating centers and was conducted according to the principles ofthe Helsinki declaration http://www.hqlo.com/content/5/1/65 We consulted the Dutch national RB register [11] to collect ... research, collected and analyzed the data and drafted the manuscript SMI, ACM and JH participated inthe design ofthe study, interpreted the data and revised the manuscript AYNSM, PJR and PTCK interpreted ... Health Organization MD Mean Differences PTG Post Traumatic Growth Competing interests The author(s) declare that they have no competing interests Authors' contributions JvD has coordinated the...
... in data acquisition, subject recruitment and drafting the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... with diabetes may not be able to detect small perturbations ofthe surface on which they stand or that it takes a longer movement distance before they detect the onset of a slip Both actions could ... efficient and provides information not only ofthe main effects ofthe factors but also of their combined interactions Since we have unequal sample sizes, to satisfy the orthogonality of the...
... (5') and EcoRI (3') restriction sites The upstream primer sequence was 5'CAGATCTCATGTTTCAAGACACTGAGGAAAAACCAC while the downstream primer sequence was 5'CAGAATTCGTCACAGTGCAGTTTCT-CTACGTCGG The ... 3, C to TT to G A to CC to A C to T A to C G to T A to C A to C A to TC to TC to T A to V L to R K to N P to T F to F (none) I to L V to L N to H K to N R to R (none) A to V S to S (none) Codon ... the technical direction of MT, LB, and GM who also participated inthe design ofthe study, data analysis and writing the manuscript, FC provided the information contained in Table and direction...
... ofthe participating centers and was conducted according to the principles ofthe Helsinki declaration http://www.hqlo.com/content/5/1/65 We consulted the Dutch national RB register [11] to collect ... research, collected and analyzed the data and drafted the manuscript SMI, ACM and JH participated inthe design ofthe study, interpreted the data and revised the manuscript AYNSM, PJR and PTCK interpreted ... Health Organization MD Mean Differences PTG Post Traumatic Growth Competing interests The author(s) declare that they have no competing interests Authors' contributions JvD has coordinated the...