dnag gene product from escherichia coli
Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
... formation Structural properties of the NarJ chaperone E coli NarJ is a member of a large family of dedicated chaperones involved in the biogenesis of metalloproteins, including TorD, DmsD and YcdY ... overall quality of multiple alignments, 21 sequences related to NarJ from the NR databank were selected by a single Blast search from the NCBI server These sequences were used to derive multiple ... Magalon A & Mendel RR (2008) Biosynthesis and insertion of the molybdenum cofactor In EcoSal Escherichia coli and Salmonella: cellular and molecular biology (Bock A, Curtiss R III, Kaper JB, ¨ Karp...
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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... Sigurskjold BW, Christensen U & Willemoes M (2005) Structures of dCTP deaminase from Escherichia coli with bound substrate and product: reaction mechanism and determinants of mono- and bifunctionality ... pETDCD described previously [18] was used as a template for mutagenesis The pETDCD plasmid contains the reading frame of the E coli dcd gene under control of the late T7 promoter in the vector pET11a ... above for samples from kinetic experiments Data from presteady-state and steady-state kinetic and equilibrium binding experiments were analysed using the computer program ultrafit from biosoft (v...
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Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... expression vector, pET28a(+) was from Novagen E coli competent cells, XL1-blue (for cloning), and BL21 (for protein expression) were purchased from Stratagene Site-directed mutagenesis was performed using ... 2¢-O-anthraniloyl (ant-cAMP) was from Calbiochem Calf intestine alkaline phosphatase was from Takara Bio DEAE Sephadex was from Amersham Biosciences Other chemicals were from Wako Pure Chemicals Spectral ... sensor from Escherichia coli J Biol Chem 277, 32650–32658 17 Watanabe, M., Matsui, T., Sasakura, Y., Sagami, I & Shimizu, T (2003) Unusual cyanide binding to a heme-regulated phosphodiesterase from...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... 3-uorotyrosine-containing cyclic AMP receptor protein from Escherichia coli J Biochem 266, 545552 14 Wasylewski, M., Maecki, J & Wasylewski, Z (1995) Fluorescence study of Escherichia coli cyclic AMP receptor protein ... AMP-dependent forms of cyclic AMP receptor protein from Escherichia coli FEBS Lett 453, 215218 12 McKay, D.B & Steitz, T.A (1981) Structure of catabolite gene activator protein at 2.9 A resolution ... were purchased from Sigma cAMP and dithiothreitol were from Fluka The Fractogel EMD SO3 650 (M) was from Merck, and Q-Sepharose Fast Flow, Sephacryl S-200 HR and Sephadex G-25 were from Amersham...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... alkaline phosphatase from E coli (Eur J Biochem 270) 4357 with the flip-flop and half-of-the-sites mechanism [18] In our previous work, APase from E coli displayed deviations from Michaelis–Menten ... Fig The influence of Mg2+ on the kinetic properties of APase from E coli The influence of Mg2+ on the kinetic properties of APase from E coli at a Zn2+ to dimer ratio of : in 2A2M1P buffer (pH 10.5) ... subunits Model representation of the catalytic cycle for APase from E coli A model describing the catalytic mechanism of APase from E coli, based on the results of the kinetic experiments and in...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... manganese-superoxide dismutase of Escherichia coli J Biol Chem 266, 303–308 Ose, D.E & Fridovich, I (1979) Manganese-containing superoxide dismutase from Escherichia coli: reversible resolution and ... fused proteins in Escherichia coli Gene 69, 301–315 45 Hassan, H.M & Fridovich, I (1978) Superoxide radical and the oxygen enhancement of the toxicity of paraquat in Escherichia coli J Biol Chem ... site-directed mutagenesis Oligonucleotide site-directed mutagenesis was carried out by the phosphorothioate DNA method of Eckstein [38] utilized in the Sculptor in vitro mutagenesis kit from Amersham...
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Tài liệu Báo cáo khoa học: The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli ppt
... ATP-dependent protease La (Lon) from Escherichia coli Methods Enzymol 244, 350±375 Mizusawa, S & Gottesman, S (1983) Protein degradation in Escherichia coli: the lon gene controls the stability of ... Insertional mutagenesis of the lon gene in Escherichia coli: lon is dispensable J Bacteriol 164, 1124±1135 Maurizi, M.R (1987) Degradation in vitro of Bacteriophage kN protein by Lon protease from Escherichia ... of Escherichia coli and its negative regulation by Lon Mol Gen Genet 254, 351± 357 14 Andrade, M.A., Chacon, P., Merelo, J.J & Moran, F (1993) Evaluation of secondary structure of proteins from...
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Báo cáo khoa học: High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli pot
... Table Enzymatic activity of CysM from Escherichia coli The estimated relative errors are about 20% The OAS concentration was always 10 mM; the TNB concentration varied from 10 to 1000 lM The temperature ... Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli Biochemistry 44, 8620– 8626 5388 Maier THP (2003) Semisynthetic production of unnatural 1-a-amino acids by metabolic ... because multiple binding is usually weak The observed kinetic parameters of wild-type CysM from E coli are in general agreement with those of the homolog CysM(salmo) [18,19] Of the active center mutants...
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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... Kim KS (2001) Escherichia coli translocation at the blood-brain barrier Infect Immun 69, 5217–5222 Huang SH, Wass CA, Fu Q, Prasadarao NA, Stins MF & Kim KS (1995) Escherichia coli invasion of ... invasion gene ibe10 Infect Immun 63, 4470–4475 Huang SH, Wan ZS, Chen YH, Jong AY & Kim KS (2001) Further characterization of Escherichia coli brain microvascular endothelial cell invasion gene IbeA ... bacterial porin from random coil to native conformation J Biol Chem 265, 10217–10220 Rocque WJ & McGroarty EJ (1990) Structure and function of an OmpC deletion mutant porin from Escherichia coli K-12...
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Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx
... region of the Escherichia coli nitrate reductase (nar) operon contains two genes J Bacteriol 170, 1721–1729 Sodergren, E.J., Hsu, P.Y & DeMoss, J.A (1988) Roles of the narJ and narI gene products ... nitrate reductase in Escherichia coli J Biol Chem 263, 16156–16162 Blasco, F., Iobbi, C., Giordano, G., Chippaux, M & Bonnefoy, V (1989) Nitrate reductase of Escherichia coli: completion of the ... in Escherichia coli Studies with a ubiA–menA– double quinone mutant Biochem Biophys Acta 461, 84–100 Morpeth, F.F & Boxer, D (1985) Kinetic analysis of respiratory nitrate reductase from Escherichia...
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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... sequencing kit was from PerkinElmer (Boston, MA, USA) Cloning of the E coli relA gene The relA gene was amplified by PCR from E coli MRE 600 genomic DNA with primers 5¢-CGGGAATTCCATATGGT TGCGGTAAGAAT-3¢ ... ribosomes from Escherichia coli Biochemistry 25, 3245–3255 Justesen J, Lund T, Pedersen FS & Kjeldgaard NO (1986) The physiology of stringent factor (ATP:GTP 3¢ diphosphotransferase) in Escherichia coli ... ribosomes from Escherichia coli Biochemistry 23, 6710–6717 31 Gao W, Jakubowski H & Goldman E (1995) Evidence that uncharged tRNA can inhibit a programmed translational frameshift in Escherichia coli...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... cAMP receptor protein from Escherichia coli J Biol Chem 278, 4302043026 Baszczyk U, Polit A, Guz A & Wasylewski Z (2002) Interaction of cAMP receptor protein from Escherichia coli with cAMP and ... protein from Escherichia coli Eur J Biochem 270, 14131423 Yu S & Lee JC (2004) Role of residue 138 in the interdomain hinge region in transmitting allosteric signals for DNA binding in Escherichia coli ... were purchased from Sigma N-Iodoacetylaminoethyl-1-naphthylamine-5-sulfonate (1,5-I-AEDANS) was obtained from Molecular Probes (Eugene, OR) The nutrients for bacterial growth were from Gibco BRL...
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Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx
... amplified by PCR using genomic DNA isolated from Escherichia coli strain JM109 as a template The Fe(II) Ec DosH proteins (containing nearly 10 lm heme) were generated by reduction with sodium dithionite ... 10 Hz) from a xenon lamp with the light intensity reduced by as much as 80% at Delgado-Nixon VM, Gonzalez G & Gilles-Gonzalez MA (2000) Dos, a heme-binding PAS protein from Escherichia coli, is ... sensor from Escherichia coli Biochemisty 41, 8414–8421 Balland V, Bouzhir-Sima L, Kiger L, Marden MC, Vos MH, Liebl U & Mattioli TA (2005) Role of arginine 220 in the oxygen sensor FixL from Bradyrhizobium...
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Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli ppt
... FixL from Rhizobium meliloti: crystallographic, mutagenesis and resonance Raman spectroscopic studies J Mol Biol 301, 415–431 32 Myer, Y.P & Bullock, P.A (1978) Cytochrome b562 from Escherichia coli: ... isolated heme domain of a direct oxygen sensor from Escherichia coli J Biol Chem 277, 32650–32658 Jackson, F.R., Bargiello, T.A., Yun, S.H & Young, M.W (1986) Product of per locus of Drosophila shares ... was purchased from Calbiochem (La Jolla, CA, USA) Calf intestine alkaline phosphatase was purchased from Takara Syuzo Co (Otsu, Japan) DEAE-Sephadex and Sephadex G25 were obtained from Amersham...
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Báo cáo khoa học: A periplasmic aldehyde oxidoreductase represents the first molybdopterin cytosine dinucleotide cofactor containing molybdo-flavoenzyme from Escherichia coli docx
... biosynthesis in E coli requires a so far unidentified gene product that catalyzes the attachment of CMP to MPT Our investigations further showed that the yagQ gene product is required for the production ... In general, Moco formed in Escherichia coli is further modified by covalent addition of GMP to the phosphate atom on C4¢ of MPT via a pyrophosphate bond, a reaction catalyzed by the mobAB gene products ... expression and purification of E coli YagTSRQ DNA fragments containing the coding regions for E coli yagTSRQ were amplified by PCR from total DNA obtained from E coli K-12 The flanking restriction...
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Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx
... Mitra et al D97 mutants of the MetAP-I from E coli genes in Escherichia coli, Salmonella typhimurium, and Saccharomyces cerevisiae [710] Moreover, a MetAP from eukaryotes has been identied as the ... aminopeptidase from Escherichia coli Biochemistry 39, 38173826 23 Cosper NJ, DSouza V, Scott R & Holz RC (2001) Structural evidence that the methionyl aminopeptidase from Escherichia coli is a mononuclear ... using the Quick Change Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA), following Stratagenes procedure Reaction products were transformed into E coli XL1-Blue competent cells (recA1...
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Báo cáo Y học: Contribution of Lys276 to the conformational flexibility of the active site of glutamate decarboxylase from Escherichia coli pptx
... glutamate decarboxylase from Escherichia coli J Biol Chem 273, 1939–1945 22 Higuchi, R., Krummel, B & Saiki, R.K (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: ... from Pharmacia [a-35S]dATP (1000 CiÆmmol)1) was from New England Nuclear Ingredients for bacterial growth were from Difco Oligonucleotides were from Genenco GuanidineÆHCl, 2,2,2-trifluoroethylamine, ... decarboxylation, and product release steps Biochemistry 38, 11814–11826 13 De Biase, D., Tramonti, A., Bossa, F & Visca, P (1999) The response to stationary-phase stress conditions in Escherichia coli: role...
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Báo cáo y học: " Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli" pptx
... have reported on the production of successful Cap protein VLPs in Escherichia coli (E coli) [7] This is the first report on the production of Cap protein VLPs of PCV2 in E coli The small ubiquitin-like ... expression in E coli Lane M, molecular weight marker; lane 1, bacterial lysates from cells without IPTG; lane 2, bacterial lysates from IPTG-induced cells in E coli; lane 3, pellet from bacterial ... porcine circovirus ORF2 gene requires codon optimized E coli cells Virus Genes 2007, 34:199-204 Pryor KD, Leiting B: High-level expression of soluble protein in Escherichia coli using a His6-tag...
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Báo cáo y học: "Chromatin architecture and gene expression in Escherichia coli" pdf
... two studies on gene expression in human chromosomes that showed clustering of highly expressed genes [6,7] The topic of chromatin structure and gene expression in eukaryotes has generated considerably ... On the structure of the folded chromosome of Escherichia coli J Mol Biol 1972, 71:127-147 Sinden RR, Pettijohn DE: Chromosomes in living Escherichia coli cells are segregated into domains of supercoiling ... highly expressed genes are localized near the replication origins and the region around the replication terminus contains few highly expressed genes This was thought to be a result of gene dosage...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... (fig 5) Fig 5: Analysis of MCoTI-II gene on 5%polyacrylamide gel Lane 1: DNA Marker 100 bp (100-1031) (#SMO241/2/3) Lane : PCR product of MCoTI-II gene MCoTI-II gene was also successfully prepared ... sequence of synthetic MCoTI-II gene Amplification of MCoTI-II gene by PCR The PCR conditions were established as :940C,3 ; 610C 1min30;720C ,2 for 40 cycles The PCR product showed a single band ... Transformed into E .coli BL21 (DE3) PI - 17 Fig 7: Scheme summarizing the steps in obtaining a construct system for expression of MCoTI-II To identify the insert gene, we isolated plasmid from PI- 17,...
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