... Outline ofthe Good Life, and What We Can Do at Little Cost 6.1 A Broad Outline of a Theory ofthe Good 6.1.1 Some Rather Formal Remarks on the Good 6.1.2 A Theory ofthe Good in Broad Outline ... mechanical, photocopying, recording or otherwise) without the written permission of both the copyright owner and the author ofthe book Affluent intheFaceof Poverty On What Rich Individuals Like ... questions is by examining the most important forms of moral theorizing, as the forum where some ofthe most central moral insights are considered Also, moral theorists often consider their theory to support...
... Alpine region hampering the start remained at the same level as 1998 A ofthe season Cement consumption is slight decline in operating profit was likely to rise further inthe second half of ... first- weakened further inthe first half ofthe year, but the decline was within a far ditions inthe second half of 1999 and narrower range than in 1998 Business the Swiss franc remains at its present ... during the second half of 1999 Events Subsequent to June 30, 1999 Within the scope of optimizing plant location, Alsons Cement inthe Philippines sold its Kiwalan factory at the beginning of the...
... maintain transcription The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain showing the same architecture as the DIDO1 long isoform, although lacking the dPHD The ... To root the tree, we used inhibitor of growth protein (ING3) PHD, which is another domain involved in chromatin binding and clearly more divergent (e-value 0.019) from DIDO and the other sequences ... from the other branches, which was indeed predicted The other main group, obtained 97% ofthe time in 20 740 explored trees, contained the DIDO PHD sequences clustered with the PHF and the CGBP...
... and 1L8Q (DnaA) The AAA+ domains of both proteins are shown in cyan with ADP indicated in red The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix ... confirmed the position ofthe origin of replication in Pyrococcus, providing the first experimental proof of a localized origin of replication inthe archaea [52] Interestingly, in a situation reminiscent ... seen in Fig 4, both proteins possess N-terminal AAA+ domains and C-terminal DNA binding domains (DBDs) In DnaA, the DBD contains a helix-turn-helix; inthe archaeal proteins, the DBD has a winged...
... destabilization and hence, diminution ofthe melting temperature (Tm) inthe former Base stacking inthe vicinity of A C mismatch in PNA DNA and DNA duplexes Intra strand base stacking at the AC(6–7) (Fig ... variableness inthe water interactions surrounding the mismatch Water interaction inthe vicinity of A C mismatch Figure 7A–L depicts the nature of water interaction inthe neighbourhood of A24 C7 ... and water organization inthe vicinity ofthe mismatch might be the contributing factors for the increase in free energy and diminished stability of ` PNA DNA vis-a-vis DNA duplex FEBS Journal...
... within them, generating genomic DNA within the cytoplasm of each cell type These viral DNA motifs are then recognized by DAI, leading to the activation of downstream effector molecules including ... drafted the manuscript VSC participated inthe performance ofthein vivo viral infections and the isolation of CNS cells MM participated inthe preparation of viral stocks and determination of viral ... primarily by infiltrating leukocytes or by the responses ofthe resident cells ofthe CNS themselves In support ofthe latter possibility, activated glial cells are known to be capable of producing toxic...
... version of Theorem 1.1 Theorem 3.4 If G embeds inthe projective plane with face width at least r ≥ 6, then the crossing number crg (G) of G inthe plane is at least 36 r Estimating the crossing ... embeds inthe projective plane with face width r Then the crossing number of G inthe plane (and thus in any orientable surface) is at most r ∆2 /8 Proof Consider , the dual edge-width of G—i.e the ... case the number of crossings in D is within a constant factor ∆2 /(8cg ) of crg (G) Remark 4.2 Inthe planar case of Theorem 1.2, the described approximation algorithm yields a drawing of G within...
... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... DH, Shin JH An occurrence of canine herpesvirus (CHV) infection in Korea Korean J Vet Res 1992, 32, 217-225 Reading MJ, Field HJ A serological study of canine herpes virus-1 infection inthe English ... proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe inthe hybridization assay for the detection of...
... Consequently, the open-air drying process results not only in highly degraded DNA but also inthe apparition of molecules inhibiting genetic analyses (mainly PCR) Finally, during barrel making, the central ... with the predicted one, see [5]) The results (determination ofthe haplotype of each of these eight samples) can then be compared with the genetic composition ofthe announced provenance using the ... al the effect ofthe geographic origin of oak wood on wines is the subject of many discussions and experimentations throughout the world [10, 13, 14], along with the influence of barrel making...
... such lineages were Page 10 of 13 included in this survey Other than hatching (and the conditions immediately preceding hatching), sexual sublines were maintained inthe same manner over the course ... resulting from the unmasking of recessive, negative effects oftheDNA transposon once the insert is present at the same locus on both chromosomes The second is the dramatic reduction inthe probability ... and the back-up individuals were dead, the line was propagated from beakers of animals from previous generations ofthe lineage also maintained inthe lab (at 10°C) by selecting a random individual...
... seemingly paradoxical result can be explained inthe following way The chance of finding a seed-match and of producing a local alignment of significant similarity rises with the length ofthe ... tractable for informative ancient DNA shotgun sequencing Results To assess the biases introduced inthe analyses of ancient DNA, we use a subset ofthe sequence data generated as part ofthe Neandertal ... the nucleotide misincorporation inthe Neandertal sequence) Assuming a molecular clock, the ratio ofthe number of changes specific to the human lineage to those specific to the chimpanzee lineage...
... allowing the distinction of 22 haplotypes belonging to the three Mediterranean cpDNA lineages known as E1, E2 and E3 The discriminating power of cpDNA variation was particularly low for the cultivated ... recently investigated using different molecular techniques, including looking at organelle variation [10-15] These previous studies allowed the detection of seven main cpDNA lineages inthe O europaea ... challenging, limiting the applications of such an approach Inthe present study, we screened cpDNA polymorphism inthe olive tree (Olea europaea L.) by sequencing the complete plastid genome of trees...
... I after SUMO1 inhibition inthe fibroblasts According to the intensity ofthe bands of remaining supercoiled DNAin serial dilutions inthe assays of three fibroblast strains, these changes are ... amounts of topo I inthe nuclear extracts inthe relaxing assays The efficiency of SSc topo I in relaxing the supercoiled DNA appeared to be less than that of control topo I in each concentration of ... gradually diminished following increased amounts of topo I inthe nuclear extracts Based on the intensity of supercoiled DNA bands that were correlated with the amounts of topo I inthe nuclear...
... accommodated inthe VG interaction, but rather inthe residual error The remaining 14.9 % variance inthe VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences ... the number of members, and their (putative) functions are listed to the right ofthe corresponding clusters The minimum number of genes in an operon of which all members should be in a certain ... for the VG interaction, followed by the experimenter, theDNA microarray batch, and lastly a change inthe RNA isolation method (coinciding with the use of arrays spotted with 12 instead of spot-pins)...
... vaccines, each encoding the same antigen, administered some weeks apart Inthe prevention of TB, the prime-boost strategy of combining DNA priming and boosting with BCG or recombinant proteins has been ... technique may increase veterinarian and patient acceptance of vaccination by reducing the number of injections and avoiding the use of boosters containing live vectors Using this technology, vaccinologists ... used in each group For naked DNA vaccination, the plasmid DNAhsp65 was administered by intramuscular injection of 100 μg DNAin saline into each quadriceps muscle on three occasions at 2-week intervals...
... to find intein insertions by the TIGRFAM profiles TIGR01445 (intein amino terminus) and TIGR01443 (intein carboxyl terminus) [65], but none of these fragments had a detectable intein domain In ... least 3% ofthe predicted proteins contained within the GOS data are of viral origin Notably, a number of sequences most similar to the genome ofthe giant mimivirus have been found inthe Sargasso ... contig was defined by dividing the cumulated size of reads contributing to the contig by the size ofthe contig Relative abundance of PolBs For the analysis ofthe relative abundance of PolB sequences,...
... could also be the result of R2's reliance upon co-transcription with the rDNA units in which they reside Second-strand DNA synthesis Many ofthe initial steps involved inthe cleavage ofthe target ... Summary ofthe nascent lineages of R1 and R2 in two species Summary ofthe nascent lineages of R1 and R2 in two species In both panels the elements are diagramed as in Figure 2b Values below the ... anneal a few nucleotides ofthe cDNA strand to the top strand ofthe target DNA before initiating secondstrand DNA synthesis would explain the frequent 'microhomologies' between internal R2 sequences...
... the pathway is initiated by the Ku protein binding damaged DNA termini, translocates inward, and recruits DNA- PKcs to form the DNA- PK heterotrimer DNA- PK is activated as a serine-threonine kinase ... the C-terminus of Ku80 is essential for efficient activation of DNA- PKcs, and only the final 12 amino acids are sufficient to bind DNA- PK (31-33) Upon removal ofthe C-terminus of Ku80 the kinase ... can be removed via thrombin cleavage DNA Binding of Ku is Independent ofthe Ku80CTR – DNA binding ofthe purified Ku variants was assessed in an EMSA using a 30-bp duplex DNA 30A and 30C (Table...
... sentences, the thing doing the action is the subject ofthe sentence and the thing receiving the action is the object Inpassive sentences, the thing receiving the action is the subject ofthe sentence ... ofthe errors 3.1 Interlingual errors These errors are found inthe misselection of voice One ofthe factors leading to these errors is that the students have a habit of thinking in their mother ... Confusion of verb tenses When transforming a sentence from the active into the passive, the students not keep the tense oftheofthepassive sentence equivalent to the tense ofthe active ones...