... 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase in the range of the binding ... K. , Kaya, S., Abe, K & Mardh, S (2001) The oligomeric nature of Na+ /K+ -transport ATPase J Biochem (Tokyo) 129, 335342 13 Karlish, S.J.D (1980) Characterization of conformational changes in (Na, K) -ATPase ... R.W (2000) Contribution of quarternary protein interactions to the mechanism of energy transduction in Na+ /K+ -ATPase In Na/ K- ATPase and Related ATPases (Taniguchi, K & Kaya, S., eds), pp 349356...
... oligomeric nature of the enzyme J Biochem (Tokyo) 116, 1360–1369 ˚ Taniguchi K, Kaya S, Abe K & Mardh S (2001) The oligomeric nature ofNa ⁄ K- transport ATPase J Biochem (Tokyo) 129, 335–342 Abe K, Kaya ... ions with Na+ ⁄ K+ -ATPase IV Affinity change for K+ and Na+ of Na+ ,K+ -ATPase in the cycle of the ATP hydrolysis reaction J Biochem (Tokyo) 109, 70–77 17 Arato-Oshima T, Matsui H, Wakizaka A & Homareda ... inactivated ATPase On the other hand, omission of NaCl completely inactivated ATPase (Fig 9B) 677 Stimulation of pNPPase activity of Na+ ⁄ K+ -ATPase Fig Na+ -dependent activation curves for Na+ ...
... ouabain-insensitive isoform of Na+ /K+ -ATPase In most tissues, the Na+ /K+ -ATPase is a heterodimer consisting of a 112 kDa hydrolytic a-peptide, through which the vectorial transport of Na+ and K+ takes place, ... -insensitive a-isoforms of Na+ /K+ -ATPase in rats All isoforms of the catalytic a-peptide of Na+ /K+ -ATPase have similar, high affinity for ouabain [17] with Kd in the order of one or a few nanomolar [12,17] ... in Na, K- ATPase isoform expression in rat cardiac ventricle J Biol Chem 266, 9327–9331 26 Hansen, O (2000) Real concentrations of isoforms of Na, KATPase from western blots of tissue homogenates...
... forms Effects of pressure on ¯uorescence probes Fig Eect of pressure on the intensity and kmax of intrinsic ¯uorescence of membrane-bound Na+ /K+ -ATPase (A) and solubilized Na+ / K+ -ATPase (B) Ten ... thickness and cross-sectional area of the lipid bilayer of the present pig kidney Na+ /K+ ATPase is dif®cult because the precise lipid composition is unknown Generally, phospholipids of pig kidney ... domain of Na+ , K+ -ATPase: The tetrameric nature of the enzyme J Biol Chem 273, 24339±24345 41 Tsuda, T., Kaya, S., Yokoyama, T., Hayashi, Y & Taniguchi, K (1998) Half site modi®cation of Lys-480 of...
... functions of the NKA isoforms in skeletal muscle NKA Function isoform α1 Key housekeeping isoform, large contribution to Na+ /K+ exchange mainly during basal conditions α2 Key α isoform in skeletal muscle, ... of these isoforms include global knockout models, which are not successful in helping determine functions of each isoform, as a knockout of either of the three α isoforms leads to the death of ... phosphorylation via protein kinase A had no effect on the K+ affinity of α1/β1isoforms Activation by PKA phosphorylation alone of PLM influences the apparent Na+ affinity of the Na+ , K+ -ATPase α1/β1 and...
... protection of the membrane enzyme Na/ K- ATPase from inactivation by the heat shock protein a-crystallin Na/ K- ATPase activity decreased upon incubation with fructose, H2O2 and methylglyoxal However, Na/ K- ATPase ... specific inhibitor of Na/ K- ATPase, caused a 35% decrease in the 86Rb uptake within the ghost cells This demonstrates that the ghosts have functional Na/ KATPase The presence of a-crystallin within ... the supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose...
... activation of p42/44 MAPK (also called ERK1/2) through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation of this ... Role of protein kinase C in the signal pathways that link Na+ / K+ -ATPase to ERK1/2 J Biol Chem 276, 42050–42056 32 Tian, J., Gong, X & Xie, Z (2001) Signal-transducing function of Na+ -K+ -ATPase ... independence of low K+ induction of Na, K- ATPase: Possible role of c-fos J Membr Biol 125, 163–170 10 Nakagawa, Y., Rivera, V & Larner, A.C (1992) A role for the Na/ K- ATPase in the control of human...
... loài Penaeus aztecus, Penaeus duorarum, Penaeus setiferus, Penaeus stylirostris Áp suất thẩm thấu nhóm có độ đẳng áp với môi trường nước biển 745 mOsm/Kg, 768 mOsm /K g, 680 mOsm/Kg 699 mOsm/Kg Áp ... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro 1998 ... thải nước khỏi thể Cơ chế vận chuyển ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland)...
... loài Penaeus aztecus, Penaeus duorarum, Penaeus setiferus, Penaeus stylirostris Áp suất thẩm thấu nhóm có độ đẳng áp với môi trường nước biển 745 mOsm/Kg, 768 mOsm /K g, 680 mOsm/Kg 699 mOsm/Kg Áp ... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro 1998 ... thải nước khỏi thể Cơ chế vận chuyển ion Na Cl vào thể thải môi trường xác định, chế tiến hành khả thấm nước bề mặt + + thể tham gia vài enzyme Na /K ATPase mang tuyến râu hàm (antenal gland)...
... subunit of Na+ /K+ ATPase in HEK293 cells [24,25] In Sf9 cells, however, expression of H+ /K+ -ATPase a subunit without a corresponding b subunit produces a ouabain-insensitive H+ /K+ ATPase [26] Finally, ... measured Na+ - and K+ -stimulated ATPase activity found in the apical membranes of the colon is not due to Na+ / K+ -ATPase, but could be associated with some form(s) of the colonic H+ /K+ -ATPase, ... immunoreactivity of the a1-subunit of the Ó FEBS 2002 Palytoxin action on colonic H+ /K+ -ATPase (Eur J Biochem 269) 3909 Fig Immunohistochemical detection of the a1-subunit of the Na+ /K+ -ATPase in rat...
... [-] 0.5 0.4 0.5 0.4 0.3 0.3 0.2 0.2 Na 0.1 Ca Mg K Cl Na 0.1 Ca Mg K Cl 0 PV [-] PV [-] 10 Fig 13 - Breakthrough curves of all ions Fig 14 - Breakthrough curves of all ions (0.5 wt%) (1.0 wt%) The ... time ACKNOWLEDGEMENTS We would like to express our appreciation to Professor Sho Shiozawa, Professor Masaru Mizoguchi and Mr Hiromi Imoto of the University of Tokyo for their assistance in making ... conductivity between porous cups was determined as K1 -2, K2 -3, K3 -4 and the total hydraulic conductivity was determined as K1 -4 The changes in K1 -2, K2 -3, and K3 -4 were high during the initial stage, from...
... gestational doses of 127–151 mg/kg bw/day and higher and a lactational dose of 166−377 mg/kg bw/day and higher; the developmental NOAEL is in the range of 38–44 mg/kg bw/day (gestational) and ... maternal and developmental toxicity at 1,000 mg/kg bw/day and a NOAEL of 500 mg/kg bw/day The Expert Panel concurred with the maternal NOAEL but selected a developmental NOAEL of 100 mg/kg bw/day ... assessment in developmental toxicity Risk Analysis 12:439-447(1992) 33 McKee R Personal communication to Jack Moore, 2000 34 Hellwig J, Freudenberger H, Jackh R Differential prenatal toxicity of branched...
... Ivan E Brick & Tavy Ronen have appeared in The American Economic Review, The International Journal of Finance, The Journal of Banking and Finance, The Journal of Finance, The Journal of Financial ... Journal of Financial & Quantitative Analysis, The Journal of Industrial Economics, The Journal of Money, Credit & Banking, The Journal of Political Economy, Management Science, and The Review of ... Order Book Inevitable?” Journal of Finance 49, 1127–1161 (1994) Viswanathan, V and J J D Wang, “Market Architecture: Limit Order Markets Versus Dealership Markets.” Journal of Financial Markets 5,...
... ingestion of 0.38 kg canned food/day with 20 µg/kg (~0.85 µg/kg bw/day) 0.8 + 0.85 = 1.65 (26) (24 – 27) 13 assumes body weight of 7.8 kg, formula intake of 920 ml/day, and food consumption of 0.407 kg/day ... Park, NC at the National Institute of Environmental Health Sciences, a component of the National Institutes of Health CERHR follows a formal process for review and evaluation of nominated chemicals ... report of increased expression of cytokeratin 10 (CK10), a cell-marker associated with squamous differentiation, in adult male offspring of pregnant mice orally treated with 0.020 mg/kg bw/day...
... γj ,k = θj ∂gj ,k + 2gj ,k ∂θj Straightforward calculations yield (2.2) i i∂∂ψε = ∂ψε = j ,k θj gj ,k γj ,k , e2ψ + ε2 j ,k γj ,k ∧ γj ,k e2ψ + ε2 +i j ,k − j ,k θj gj ,k γj ,k (e2ψ ∧ + j ,k θj gj ,k γj ,k )2 ... sequence Tk = α + i∂∂ kof closed (1, 1)-currents such that (i) k (and thus Tk ) is smooth on the complement X Zk , and the Zk ’s form an increasing sequence Z0 ⊂ Z1 ⊂ ⊂ Zk ⊂ ⊂ X Zk of an analytic ... consequence, Tk converges weakly to T as k tends to +∞ (iv) Near Zk , the potential k has logarithmic poles, namely, for every x0 ∈ Zk , there is a neighborhood U of x0 such that k (z) = k log |gk, |2...
... structure of the chloroplast F1 -ATPase at 3.2 A resolution J Biol Chem 276, 13451352 Noji, H., Yasuda, R., Yoshida, M & Kinosita, K Jr (1997) Direct observation of the rotation of F1 -ATPase Nature ... Enzymol 126, 545557 34 Kluge, C & Dimroth, P (1993) Kinetics of inactivation of the F1F0 ATPaseof Propionigenium modestum by dicyclohexylcarbodiimide in relationship to H+ and Na+ concentration: ... R & Walker, J.E (1994) Structure at 2.8 A resolution of F1 -ATPase from bovine heart mitochondria Nature 370, 621628 Stock, D., Leslie, A.G & Walker, J.E (1999) Molecular architecture of the...
... Press, Cold Spring Harbor, New York Ueoka-Nakanishi, H., Tsuchiya, T., Sasaki, M., Nakanishi, Y., Cunningham, K. W & Maeshima, M (2000) Functional expression of mung bean Ca21/H1 anti-porter in ... Oesterhelt, D & Ikeda, M (1995) Molecular cloning of cDNAs (D50528, D50529) encoding Acetabularia acetabulum V type ATPase, A subunit Plant Physiol 109, 337 Ikeda, M., Konishi, K. , Kadowaki, H & Moritani, ... Saccharomyces cerevisiae Methods Enzymol 194, 428 –453 Tanaka, K. , Nakafuku, M., Tamanoi, F., Kajiro, Y., Matsumoto, K & Toh-e, A (1990) IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein...
... presence of unlabelled CT probe Acknowledgements We thank Dr J Allende for the generous gift of zebrafish CK2 clone and Dr S Moreno for the generous gift of rat recombinant PKACa and PKI inhibitor ... not a substrate of cell cycle kinases Finally, we studied the effect of H-89 inhibitor on CNBP phosphorylation because Ser158 was predicted as a cAMP-dependent protein kinase (PKA) phosphorylation ... inhibition was proportional to the amount of H-89 used (Fig S5) Because most protein kinase C (PKC) isotypes may be inhibited by H-89, we analysed the effect of the specific PKC inhibitor chelerytrine...
... groups of holim shows once more that it is sufficient to note that E2 4k+ 1 is trivial, that π2 4k+ 1 (E hK ) is finite and (5.1) π2 4k E hK → (E2 4k )K is injective for k = and and K ⊆ K2 ∩ xK1 x−1 ... out, and we must show π0 F (E hK1 , Σ4 8k E hK2 ) → HomE∗ E (E∗ E hK1 , E∗ Σ4 8k E hK2 ) is injective for k = and 1, at least for K1 of the form G2 , G24 , or Q8 and K2 of the form G24 or Q8 Since ... this The K( n)-local category Kn has internal smash products and (arbitrary) wedges given by X ∧Kn Y = LK(n) (X ∧ Y ) and Xα = LK(n) ( Xα ) Kn In making such definitions, we assume we are working...
... blocking of the ATPase activating effect of glucose was not the result of a toxic effect of 2-deoxy-d-glucose, as subsequent incubation in the presence of 10 mm glucose resulted again in H+ -ATPase ... ⁄ K+ -ATPase in the plasma membrane of mammalian cells To confirm the interaction of acetylated tubulin with H+ -ATPase and the presence of the H+ -ATPase acetylated tubulin complex in membranes of ... membrane Na+ ,K+ -ATPase is inhibited by acetylated tubulin Mol Cell Biochem 216, 85–92 Casale CH, Previtali G & Barra HS (2003) Involvement of acetylated tubulin in the regulation of Na+ ,K+ -ATPase...