... Mitsuhashi A, Yamazawa K, Nagai Y, Tanaka N, Mat-sui H & Sekiya S (2004) Correlation between MUC5ACexpression and the prognosis of patients with adenocar-cinoma ofthe uterine cervix. Ann ... bonds.Reduction of these releases a C-terminal fragment(C2-H) that can be detected witha mAb against His5,and an N-terminal fragment (M-C1) that can bedetected witha mAb against myc. Both the dimer and the ... by the mAbsagainst M1.Immunoreactivity of mAbs against M1 towards a recombinant MUC5AC C-terminal cysteine-richpartTo test the reactivity ofthe mAbs against M1towards the human MUC5AC C-terminal...
... quota may take place via a variety of administrative and market-based mechanisms including private sales and quota exchanges. MS are able to determine whether transfers take place at national, ... 12,312,020,617,019,923,910,46,711,710,114,824,825,08,814,116,817,315,710,88,915,915,128,725,217,613,733,133,50,05,010,015,020,025,030,035,040,0BelgiumBulgariaCzek Rep.DenmarkGermayEstoniaIrelandGreeceSpainFranceItalyCyprusLatviaLithuaniaLuxembourgHungaryMaltaNetherlandsAustriaPolandPortugalRomaniaSloveniaSlovakiaFinlandSwedenUnited ... quota rents are assumed within a range of 5-10% for all NMS apart from Bulgaria and Romania, which remain with milk production under quota, and Poland and Hungary, with quota rents above average...
... H-bonddonor leads to rapid O2off-rates [19]. Some ofthe mostunusual Hbs characterized to date are those from nematodesand trematodes (mammalian parasites) such as the nematodeAscaris suum (As) [11,12] ... z, away from the heme normal (z axis), a is the angle between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ... [55] had proposed a distal Tyr at position E7 as the source ofthe H-bond to ligand on the basis of a partial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree...
... between that of the monomeric and the dimeric form ( see above) inmutant PufX54*. As in these last two mutants a short lagwas observed (see Table 2), apparently the presence of a stable dimer ... polypeptidesspan the membrane witha single hydrophobic a helix. Thiscircular protein scaffold binds the pigments that aremaintained in a spatial orientation that maximizes the efficiency ofthe energy ... via the plasmid pRKX (in trans) into the host Rb. s phaeroides DQx/g, deprived ofthe chromosomal copy ofthe puf operon.Table 1. Bacterial strains and plasmids. The plasmid host strain in allthe...
... substitution of Arg46on intracellular signalling The stimulation of accumulated InsPs by increasingconcentrations of AVP, was assayed for each ofthe 19mutant constructs and the dose–response characteristicscompared ... classes of antagonist, the binding of AVP was dramatically affectedby the substitution of Arg46, withthe affinity of AVP for all the 19 mutant receptor constructs decreasing between 700-fold and ... UK).Mutant receptor constructsMutation of Arg46 to each ofthe 19 encoded amino acidswas made by a PCR approach. Mutant sense oligonucle-otides (5Â-GGGGGCCTTAGGGGACGTAXXXAATGAGGAGCTGG-3Â) contained...
... Each of these variants retains a catalytic activity against yeast RNA comparable with that of parent mBS, indicating that a native conformation ispresent. A further indication ofthe similarity ... 5Â-GAGTGCGGCCGCAAGCTTGGGCTG-3Â, had an estimated Tm of 82 C. The reverse anking primer sequence, 5Â-ATATACATATGAAAGAAAG-3Â, had a calculated Tm of 42 C. The mutagenic primers for each variant are: P1 9A ... forms.However, after 48 h, a slight prevalence ofthe MxM form can be seen in the BS-RNase and P1 9A variant, whereas the L28Q and P1 9A/ L28Q variants still contain comparableamounts of MxM and...
... 11507–11512.Supplementary material The following supplementary material is availableonline:Fig. S1. The SARS N protein is phosphorylated bySRPK1 but not by Clk1 and PKA.This material is available as part ofthe ... environmental stress,mRNA metabolism is reprogrammed to adapt tostress-induced damage. Translationally stalled mRNAstogether witha number of translation initiation factorsand RNA-binding proteins are ... mock reactionswithout crosslinker. The right-hand panel shows the relative abundance (percentage) of monomer and crosslinked forms. Percentage wascalculated as 100 · (arbitrary unit of each form...
... 3.Deamination ofthe de-O,N-acylated LPS and preparation of oligosaccharides 4 and 5 The mixture of oligosaccharides obtained after alkalinedeacylation ofthe LPS (200 mg) was treated with 300 mgNaNO2in ... from other Pectinatus strains show a lowmolecular mass band ofthe same mobility as in the strain E-82164, and a ladder-like pattern, characteristic of the presence ofthe O-chain. No bands analogous ... 1.Deamination ofthe products of complete deacylation of the LPS led to the oligosaccharides 4 and 5, representingundecasaccharide and pentasaccharide fragments of oligo-saccharides 1a and/or...
... potentials.Steady-state enzyme activities The catalytic activity ofthe NR1-FAD/NADPH domainwas examined and compared withthe CPR FAD/NADPHdomain. The CPR FAD/NADPH domain retains trans-hydrogenase ... Results are the mean and standard deviation of triplicateassays. For the determination of apparent kcatvalues, experimentswere performed at a saturating concentration of NADPH (200 lM),over a ... 2A) . In the case ofthe NR1-FAD/NADPH domain, a second 2Â,5Â-ADP-Sepharose afnity step was also incor-porated in the purification scheme, taking advantage of itsnucleotide binding capacity of...
... O-polysaccharide of Pr. alcalifaciens O23 [23], and an amide ofthe same aminoacid with D-galacturonic acid (structure 2) in the O-polysac-charides of P. mirabilis O13 [24]. An amide of D-galacturonicacid ... gel-per-meation chromatography on Sephadex G-50. Analysis of the polysaccharide using an amino-acid analyzer revealed the presence of GlcN and GalN in the ratio 2 : 1 as well asanother amino component. ... (S)-2-butylglycosides. The Dconfiguration of GlcA was determined byanalysis of the 13C NMR chemical-shift data of the polysaccharide (see below). The specific optical rotation values of 2S,8R-AlaLys for2S,8S-AlaLys,...
... countries about the content of these annual reports.TABLE 3RESULTS OFTHE COURT’S ANALYSIS OFTHE CONTENT OFTHE LAST ANNUAL REPORT AVAILABLE AT THE TIME OFTHE AUDITSubject Argentina Israel IndiaNew ... analysed a sample ofthe annual reports of third countries currently recognised as equivalent. These annual reports are not com-plete as they lack information about monitoring activities, about ... composed ofa table with the number of visits carried out by the various private inspection bodies, the number of samples taken for analysis and the number of irregularities found and penalties applied....
... (d) 5Â-ATAGTTTAGCGGCCGCTTAGTGATGGTGATGGTGATGGGGCTTGCGCTGGATGAGCAGG-3Â for c1219HIS. For the e1219variants (e1220 with additional Pro), the forward pri-mer 5Â-GGTGTAGAATTCAAGAACGAGGAACTGCG-3Âwas ... 5Â-ATAGTTTAGCGGCCGCTCAATGGTGATGGTGATGGTGCTTGCGGCGGATGATGAG-3Â. For the c1218 variants (some with an additional C-terminal Progiving c1219), the forward primer 5Â-GGTGTAGAATTCCGGAACCAGGAGGAG ... solution ofthe structure of the mammalian AChR molecule. A prerequisite forthis is the availability of large amounts of native, sol-uble AChR molecules, a target that can be partiallyachieved...
... that the traditional methodological approaches, and their traditional points of focus, lack relevance today. However, the complexity ofthe changes taking place and the shifting nature ofthe ... basic premise that the increased complexity of local media ecosystems warrants the consideration ofthe full range of available analytical approaches to understanding how these ecosystems are ... personal networks. An adequate model of local community information needs in health must account for these various levels, and whether, and how, local health information is actually made available....
... the provinceinexchangefor the support of the Polesin the Austrianparliament. with a Polisharmy organizedinAmerica, of un-Amer-icanized Polishimmigrants,begananoffensiveagainst the Ukrainians,attackingthem from the rear. ... a temporaryoverthrowby the Germanmilitaryforcewasreestablished.In the latterpart of 1918, the Ukrainians of EasternGa-licia(also predominantlyUkrainianandanciently, Accordingto the InternationalEncyclopedia, the entire Austrianprovince of Galicia(westernandeast-ern) ... Ukrainians.Accordingto the EncyclopediaBrittanica, the formerpredominatein the West andin the bigtowns,and the latterin the East.Accordingtoofficial statistics of the Austrianpro-vincialgovernment of Galicia, preparedandpublishedbyleaders of Polishpolitical...
... LLPdomains may be flip-flopping between a transmembranestate and parallel association withthe inner leaflet of the lipid bilayer. On the other hand, the LLP domains maypossess different activities ... membranes. Additionally, it is the first example of a direct comparison of structure and function of an entireLLP-1 domain from the laboratory adapted HXB2 strain of HIV-1 (i.e., LLP-1) witha natural ... characterization of two activeforms, and partial cDNA sequence ofa precursor. Proc NatlAcad Sci USA 1987, 84:5449-5453.3. Zasloff M, Martin B, Chen HC: Antimicrobial activity of syn-thetic...