determination of mirna targets in skeletal muscle cells
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... against smooth muscle actin and myosin Cells were maintained in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS) and antibiotics in a humidified atmosphere containing 5% (v/v) CO2 in ... cell margins consistent with the role of jasplakinolide as a stabilizer of F-actin In contrast, treatment of the cells with swinholide A did not affect the intensity of F-actin stress fibers in the ... anisomycin or FBS and further incubating the cells with actinomycin D (10 lgÆmL)1) for the indicated periods Likewise, the role of RhoA and p38 signaling was examined by preincubating the cells...
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Báo cáo khoa học: Progressive decrease of phosphocreatine, creatine and creatine kinase in skeletal muscle upon transformation to sarcoma pot
... developed in the hind leg of mice by injecting 3MC, a chemical carcinogen, or by injecting into the hind leg existing sarcoma 180 cells, which had been previously maintained in the peritoneal cavity of ... creatine kinase in sarcoma of creatine and an increased choline ⁄ creatine ratio, and a decrease in the activity of CK and some of its isoforms in several different forms of cancer [15–20] Skeletal ... Expression of mRNA of MCK and sMitCK isoforms of creatine kinase and b-actin in normal muscle (NM) and 3MC induced sarcoma tissue (ST) of mouse Relative expression of mRNA BCK protein expression in both...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... calpain was investigated A direct, in vitro digestion of CARP by calpain using recombinant proteins could not be attempted because calpain is inactivated during purification [45] In skeletal muscle ... are involved in the transmission of mechanical signals to signaling pathways In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... major inducer of muscle atrophy in the skeletal muscle Indeed, NF-jB inhibition in mice models invariably protects against muscle atrophy, whereas NF-jB activation promotes proteolysis in vivo...
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Báo cáo y học: " Multiple P2Y receptors couple to calcium-dependent, chloride channels in smooth muscle cells of the rat pulmonary artery" ppsx
... system, using Chart 3.3 software (AD Instruments) Cumulative concentrationresponse curves to ATP were obtained in rings in the absence of antagonist (control) or in the presence of a single concentration ... each evoked this profile of responses in a similar proportion of cells Quantitative analysis of oscillating currents The decrease in the amplitude of the oscillating currents during agonist application ... Ca2+ during maintained activation of P2Y receptors Conclusion The results of the present study indicate the presence of at least two different subtypes of P2Y receptors mediating oscillating inward...
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Báo cáo y học: " Module-based multiscale simulation of angiogenesis in skeletal muscle" doc
... molecular transport of each isoform in the interstitial space, binding of VEGF 164 to HSPG in the ECM, VEGF164/120 binding to VEGFR2 at the endothelial cell surface and internalization of these ligand-receptor ... corresponding modules The calling of these Java classes involves three steps: i) The skeletal muscle object (realization of SkeletalMuscle class) will be initialized and then transferred as the input ... grid point defined within the skeletal muscle and passes the values back to the controller to be stored in the voxel field (Grid class) of the “SkeletalMuscle” object (element PO2 is defined in Grid...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... that contain results of relevance for the present work In a study aiming at characterization of the proteome of lysosomes in U937 cells, cystatin F was found as one of 15 proteins binding to immobilised ... transport of cystatin F in model U937 cells would be valuable in understanding the role of cystatin F in haematopoietic cells Further work to localize subfractions of native haematopoetic cells expressing ... cystatin F in U937 cells The high portion of intracellular cystatin F led us to fractionate cells to determine the localization of the inhibitor Initially cells were fractionated in a debris...
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Báo cáo khoa học: Heme oxygenase-1 ⁄p21WAF1 mediates peroxisome proliferator-activated receptor-c signaling inhibition of proliferation of rat pulmonary artery smooth muscle cells pot
... families of proteins: inhibitor of kinase ⁄ alternative reading frame and CDK inhibitor protein ⁄ kinase inhibitor protein The inhibitor of kinase ⁄ alternative reading frame family includes p16INK4a ... cell cycle in the G1 phase by binding to, and inactivating, cyclin–CDK complexes [29,30] The results of our PPARc inhibition of cell proliferation study reveal that activation of PPARc increases ... experiments were performed using cells between passages and To test the purity of smooth muscle cells, cells were stained with 4¢,6¢-diamidino-2-phenylindole (Invitrogen) and fluorescein isothiocyanate-labeled...
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Báo cáo khoa học: Casein phosphopeptide promotion of calcium uptake in HT-29 cells ) relationship between biological activity and supramolecular structure ppt
... only maintain Ca2+ in an absorbable form but also interact with the plasma membranes of certain cells, facilitating Ca2+ uptake 5006 Concerning the conflicting results of human studies, some in favor ... solution of the same CPP in phosphate-free or phosphate-containing KRH with no Ca2+ showed a remarkable increase of scattered light, of the order of 10 times that of the pure solvent, indicating the ... Intestinal absorption of calcium in rats given diets containing casein or aminoacid mixture: the role of casein phosphopeptides Br J Nutr 49, 67–76 38 Sato R, Noguchi T & Naito H (1986) Casein...
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Báo cáo khoa học: Calcium-induced contraction of sarcomeres changes the regulation of mitochondrial respiration in permeabilized cardiac cells doc
... Samuel JL et al (1997) Study of functional signicance of mitochondrialcytoskeletal interactions In vivo regulation of respiration in cardiac and skeletal muscle cells of desmin-decient transgenic mice ... creatine kinase reactions, including mitochondrial creatine kinase [3840] Increase of the free calcium concentration to lm resulted in some decrease of the Vmax, but its value stayed higher in ... shortening of sarcomeres hypercontraction caused by excess free calcium results in a reversible alteration of the regular arrangement of mitochondria in the cells, in the changes in the kinetics of...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... provides procedures for determination of organic compounds in finished drinking water, source water, or drinking water in any treatment stage The method is applicable to a wide range of organic compounds ... surrogate in µg/L in the water sample Ax = integrated abundance of the quantitation ion of the analyte in the sample Ais = integrated abundance of the quantitation ion of the internal standard in the ... tune and initial calibration at the beginning of each 12-hour work shift during which analyses are performed using the following procedure 10.3.1 Inject a µL aliquot of the ng/µL solution of DFTPP,...
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Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx
... decreasing the error rate and using statistical analysis for generating new rules References Timo J~irvinen 1994 Annotating 200 Million Words: The Bank of English Project In Proceedings of COLING-94 ... errors occurred during determination of apposition and the third biggest group of errors exists in sentences there one clause divides the other into two parts In experiment A, 86-88% of words become ... (except finite verbs and conjunctions) Results To evaluate the performance of parser I use two types of corpora Training corpus is used for formulating rules and preliminary testing After testing...
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Báo cáo hóa học: " Kaposi''''s sarcoma associated herpesvirus G-protein coupled receptor activation of cyclooxygenase-2 in vascular endothelial cells" ppt
... c-Jun N-terminal kinase/stressactivated protein kinase (JNK/SAPK), and protein kinase C/activator protein-1 (PKC/AP-1), all of which regulate COX-2 expression [8,10-15] The KSHV vGPCR also induces ... induces expression of COX-2 but not COX-1 in primary endothelial cells Most cell types, including HUVEC, express two COX isoforms, COX-1 and COX-2, which perform the same function of converting ... role of COX-2 in vGPCR-induced PGE2 secretion, SC-560 (COX-1 selective inhibitor), NS-398 (COX-2 selective inhibitor), or Indomethacin (Non-selective COX inhibitor) was added to vGPCR-expressing...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
... antiviral cytokine response of infected cells to a large extent Interestingly, however, induction of the chemokines IP-10 and IL-8 escaped suppression by SARS-CoV in Caco-2 cells, but not in HEK 293s ... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order ... Figure Interleukin production Interleukin production RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RTPCR for the presence of IL-6 and IL-8 mRNAs by infected...
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Báo cáo hóa học: " Research Article Sequential Monte Carlo Methods for Joint Detection and Tracking of Multiaspect Targets in Infrared Radar Images" pptx
... n of the joint posterior distribution of the target’s kinematic and aspect states conditioned on all observed frames from instant up to instant n Given, however, the inherent nonlinearity of ... maximum of NT targets in the field of view of the sensor at each time instant, then zn would take K = 2NT possible values corresponding to the hypotheses ranging from “no target present” to “all targets ... simulations in Section in the sense that, rather than performing joint detection and tracking of the two targets, the algorithm assumes a priori that two targets are always present in the scene...
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Báo cáo khoa học: "Genetic determination of vessel area in oak (Quercus robur L and Q petraea Liebl): a characteristic related to the occurrence of stem shakes" docx
... title «Genetics and breeding of (1986) Oak breeding in Germany; experiences and problems In: Proceedings IUFRO Joint Meeting of Working Parties on Breeding Theory, Progeny Testing and Seed Orchards ... Genetic variations in wood properties In: New Perspectives in Wood Anatomy (Baas P, ed), Martinus Nijhoff, The Hague, 151-169 Cinotti B (1991) Recherche de propriétés intrinsèques du bois pouvant ... determine provenance variations in vessel size; 2) estimate the heritability of vessel size; and 3) to find a means of recognizing shake-prone trees so that they could be removed during early thinning...
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Báo cáo y học: "Elucidation of the potential roles of matrix metalloproteinases in skeletal biology" pps
... substitution of an arginine by a histidine (R101H) In affected members of both families there was no MMP-2 activity detected in serum by gelatin zymography, in contrast to measurable levels in unaffected ... disruption of the MMP13 gene The strategy employed involved the deletion of the critical zinc-binding region in the catalytic domain that resulted in markedly decreased transcription of the MMP13 ... demonstration of a spontaneous mutation in a MMP gene resulting in a human disease We look forward to descriptions of mutations and the resultant phenotype in other MMP genes geting mutations in Col1a1...
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báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant " ppsx
... 1986, 9:157-160 28 Zhang W-H, Rengel Z, Kuo J: Determination of intracellular Ca2+ in cells of intact wheat roots: loading of acetoxymethyl ester of Fluo-3 under low temperature Plant J 1998, ... of olive flower development, we observed the following: (1) the beginning of exudate production and secretion by papillae cells and (2) accumulation of lipids, pectins, arabinogalactan proteins, ... solution of mM Fluo-3 AM in DMSO The final DMSO concentration in the incubation solution was approximately 1% (v/v) Injection was done directly into the peduncle of the inflorescence at the site of...
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Báo cáo y học: "Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells" pdf
... apoptotic cells in percent of total cells The tests were performed according to the protocols of the manufactures Determination of caspase activities Activities of caspases 3, and were determined in ... the increase of the TNFα concen- tration in macrophages and TIIcells In response to hyperoxia, TNFα increased in TIIcells 1.45-fold and in macrophages 1.87-fold compared to control In TIIcells, ... Figure Hyperoxia increases the expression of TNFRI but not of Fas in TIIcells Hyperoxia increases the expression of TNFRI but not of Fas in TIIcells For the expression analysis of TNFRI and Fas...
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Báo cáo y học: "Enigma variations: control of sexual fate in nematode germ cells" docx
... elegans, including spermatogenesis It binds the transmembrane receptor TRA-2 and inhibits its activity in XO animals, causing male development The inactivation of TRA-2 permits three interacting cytoplasmic ... fascinating questions, many of which can be answered by studying other Caenorhabditis species Since the genomes of many of these species are now being sequenced, the future should be exciting interactions ... hermaphrodites too the FEM proteins are required to maintain the ability to produce sperm The FEM proteins are therefore active in the germlines of both sexes in C briggsae But where in the developmental...
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ASTM D5453 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
... uniform injection of the sample into the boat by wicking any remaining drops of the sample from the tip of the syringe needle prior to introduction of the sample into the furnace Consult instrument ... Time of Sample in Furnace—Insert the boat into the furnace using a drive rate of 140 to 160 mm/min (Model 735 setting of 700-750) Additional slowing of boat speed or a brief pause of the boat in ... Time of Needle in Furnace—Residence time of the needle in the furnace must be consistent following the injection of the sample For direct injections it is recommended that the needle remain in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25