... on the maintenance ofthe active chromatin state Results Generation of DT40/Cre cells with a long deletion in one allele ofthe Ig-b locus To genetically examine the mechanism of B cell-speci c ... 5¢-TAGATG CCGTTGTCCTCGTAGCTGATCCTG-3¢ (II: +2085 to +2114); 5¢-AGTGATGTCCTCGTAGGTGGCAATCTGC TC-3¢ (III: +3438 to +3467) (IV: same as 16 k Del) Clones whose DNA contained about kb predictable sequences ... and cloned into HindIII ⁄ NheI sites ofthe pExpress vector [45] The chicken b-actin promoterdriven puromycin resistance (Puro) gene was inserted into the XhoI site ofthe construct Selection of...
... significantly affect the intrinsic fluorescence ofthe DK9 variant, the higher the surface exposure and flexibility of their side-chains tothe solvent the smaller the intrinsic fluorescence ofthe ... which expresses the acylation rate By contrast, the decrease in the DK9 Km value is consistent with better accommodation ofthe substrate into the catalytic pocket ofthe unprotonated form ofthe ... factor) ofthe fluorescence in the absence of urea The process was highly cooperative for both thrombin species (slope factor % in both cases), and the concentration of urea inducing the 50% effect...
... DNA-binding characteristics and its activation capacity ofthe hbpC promoter in Escherichia coli Results Mutagenesis ofthe HbpR-binding sites Sequence alignment ofthe UASs found in the HbpR operators ... nucleotides of DA-hbpR were amplified by using the PCR on plasmid pHBP130 with primers hbpR5 (5¢-CGGCGGATCC.ATG.CAC.CCT.ATTCCCGATGAT), which introduces an ATG start codon in fusion with G651 of ... refer tothe locations ofthe transcriptional start site of hbpC (B) Alignments ofthe sequences of three HbpR-binding sites within the hbp gene region [19] The lane ‘cons-1’ indicates the strictly...
... CF, a critical step in bringing them tothe clinical setting Future studies should focus on the quantification ofthe effect of these correctors on the NBD1–Hsc70 interaction in the presence of ... Analysis ofthe effect of adenine nucleotides on the binding of NBD1 to Hsc70 To further explore the effect of adenine nucleotides on the NBD1–Hsc70 interaction, we quantified the effect of ATP ... the determination of whether and how correctors affect the Hsc70– F508del-NBD1 interaction, which will improve our understanding ofthe mechanism of action of small molecules with therapeutic...
... TTGGCCATATGCAGAAGATCACCGAAGCA ATTCCGGAC ACGGATCCA ATTAATCTC …20 EAMKLVAAAK-31 TCCGGCATATGGAAGCAATGAAGCTCGTC …60 TE-62 TCGCGCATATGACTGAGGATGTTGATGTT (Fig 2A) Each ofthec constructs reacted with c antiserum ... shown) The truncated polypeptides were designated cDN8 to cDN60 according tothe number of amino- acid residues deleted from thec subunit N-terminus The N-terminus amino- acid sequence ofc subunit ... impaired the stability ofthe reconstituted assemblies and the efficient assembly ofthe ab complex with recombinant c constructs; or (b) the structural change in the truncated c construct altered the...
... presented The analysis ofthe effects ofthe crown structure concentrates on the stratification ofthe crown and on thedeletionof greater segments (e.g the stem) by using the classical RBS Statistical ... auxiliary variables, the stratification ofthe crown and thedeletionof segments In the present study, the effects ofthe choice ofthe auxiliary variable and ofthe created crown structure (segments ... causes a bigger decrease in precision than for every other species The choice ofthe auxiliary variable also affects the distribution ofthe samples within the crown According to Fig 6, the cross...
... AGT CAC AAT GCG GAT CAc gtg tag gct gga gct gct tc-3’, and ΔMeq-R 5’-CTT GCA GGT GTA TAC CAG GGA GAA GGC GGG CAC GGT ACA GGT GTA AAG AGc att ccg ggg atc cgt cga c- 3’, with MDV-specific sequences ... tothe start and end ofthe coding sequence ofthe gene to be disrupted The sequences ofthe primers used for deletionof Meq were: ΔMeq-F, 5’AGA AAC ATG GGG CAT AGA CGA TGT GCT GCT GAG AGT CAC ... bac-GX0101-infected chickens And the ratio of CD4+ T cells to CD8+ T cells in bac-GX0101infected chickens significantly higher than the control group on days 21 and 28 p.i (P < 0.05) However, the...
... well conserved complex of proteins collectively known as the SCF (Skp, Cullin, F-box) The archetype ofthe SCF complex is the S cerevisiae SCF composed ofthe proteins Skp1, Cdc53, Rbx1, Cdc34, ... for the SCFGrr1 complex include the cyclins, Cln1 and Cln2 52, the meiosis activating kinase Ime2 53, the Hof1 protein required for cytokinesis 54, the Cdc42 effectors Gic2 and Gic1 55, the glucose ... in the processes of budding and cytokinesis Upon the transition from G1 to S phase ofthe cell cycle yeast cells begin to polarize the actin cytoskeleton to produce the daughter cell This process...
... to serous hip joint dysfunction Ischemic femoral head necrosis has been one of common but refractory diseases The clinical treatments of ischemic femoral head necrosis include: (1) Non-surgical ... the injured tissues once injury occurs Stem cell homing is a complicated process in which a lot of molecules were involved Once tissues were ischemic, stem cells in circulation are adherent to ... tothe vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites Inflammatory may be observed in the local ischemic tissues, and thus a lot of chemotatic...
... 200g was applied by attachinga weight tothe front chuck ofthe specimen When the test started, the back chuck constantly slided initially right to an angle of 8o then back to it’s original position ... (lactic acid) fabric Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the ... effect of time of heatsetting on tensile energy WT g.cm/cm2 of knitted Ingeo Polylactic acid fabric 3.4 Tensile resilience RT [%] Tensile Resilience RT [%] is the ability ofthe fabric to recover...
... a-helical content The transition between different environments, the membrane–cytosol inter5326 face and the cytoplasm, may affect the conformational properties of many receptor cytoplasmic tails ... shown) The lack of significant chemical shift dispersion in the HN and Ha chemical shifts is an evidence of lack of tertiary structure On the other hand, NMR spectra suggest that the conformation of ... region ofthe membrane-bound tyrosine kinase Lck with the cytoplasmic tailofthe T-cell coreceptors CD4 or CD8 [37] In solution, on the contrary, J1_tmic is mainly disordered (Figs and 3) The strongly...
... designed to target non-child audiences For tobacco, the Framework Convention86 covers actions that have the ‘aim, effect, or likely effect of promoting a tobacco product or tobacco use either directly ... Frosties Tony the Tiger, Coco Pop’s Coco the Monkey, Wall’s Max the Lion, and the characters of M&M’s Unilever’s pledge prohibits the use of cartoon characters in association with products that ... directed tothe protection of children Explore the use of standards and marketing codes to influence commercial activity, including standards from other industrial sectors Propose a set of standards...
... continuous reduction ofthe complex redox centers occurred, the anaerobic PBN adducts were rather stable Fourth, the anaerobic reaction led tothe PBN adduct being localized tothe protein fraction, ... lines of evidence point tothe presence ofthe thiyl radical in the aerobic system First, the complexes are inactivated by 2-oxo acid and CoA in the presence of O2 (Fig 2A) and the inactivation ... the integral complex structure is required for the radical species production The results ofthe current study and the site-speci c • reactivity of O2 – also bear on consideration ofthe concept...
... modifications ofthe rib cage, decreased chest-wall compliance, and increase in functional residual capacity (FRC) resulting from decreased elastic recoil ofthe lung (Fig 2) [9] The kyphotic curvature ... subjects over age 70 Lung volumes The major determinants of static lung volumes are the elastic recoil ofthe chest wall and that ofthe lung parenchyma Loss of elastic recoil ofthe lung parenchyma ... ofthe lungs is counterbalanced by an increased elastic load from the chest wall; total lung capacity (TLC) thus remains fairly constant throughout life [63] Increased elastic recoil ofthe chest...
... cytochromes c, Cys–X–X–Cys–His, was found to be located close tothe C- terminus ofthe PHCP protein, which is conserved in other biochemically characterized cytochromes c (Fig 2) The mature PHCP protein ... Heterologous synthesis of PHCP by E coli The E coli System I cytochrome c biogenesis machinery, consisting ofthe Dsb and Ccm proteins, is responsible for the synthesis of class I cytochromes c even from ... sequence deduced from the cloned gene The stretches ofthe PHCP aminoacid sequence used for the design ofthe PCR primers are underlined with arrows indicating the 5¢ to 3¢ direction The sequences...
... influence the catalytic activity of AChE and BChE We examined the possible influence ofthe C- terminal peptides on the AChE and BChE activities by comparing the catalytic rates per active site The active ... domains contribute critically tothe oligomerization and tothe efficiency of secretion Results Exchange of t peptides between human AChE and BChE The T variants of human AChE and human BChE are composed ... N1 9C in peptide b1 9C had little effect on the distribution of secreted molecular forms of Bb1 9C In the case of Ab1 9C, the effect was more complex: the cells contained mostly 4S monomers but secreted...
... deletionof FAT1: FAT1forward, ATTCTATATCTGTGAACTTTTAATAGGCTGCGAAT ACCGACTATGCGTACGCTGCAGGTCGAC; FAT1reverse, CATCCAAACCCTTTGGTAATTTTTGCTCTCT ATAAACCTTCTTCAATCGATGAATTCGAGCTCG Primers used for deletion ... deletionof FAT2: FAT2forward, GTGCTGCAAGAGGTTAGACGCTTCACGCACATTTT TGCTACAATGCGTACGCTGCAGGTCGAC; FAT2reverse, GATAGAAGCTTTCAGAGAGCATAAAATTGT ACAGGATACTGCCTAATCGATGAATTCGAGCTCG Expression of D12-desaturase ... for the accumulated fatty acids in the mutant cells, provided that the release ofthe fatty acids is rather unspeci cThe direction of fatty acid transport is dependent on the metabolic state of...
... ACTGTCAATAGTGAAGGT CCCCATGTCGCCTTTAGT TCGCTAGAACACATTGAC ATGATGAAACGGTTCAAT TTAGTTACCGTGTGCTTC CTGCTGCTCGCAGCAAGT GTGTGATCTGCAACTGTT CACCGAGGAATAATAAATGATG AAACGGTTCAATTTC TTAGTTACCGTGTGCTTC CACCGAGGAATAATAAATGATG ... Synthetic oligonucleotides used in this study Oligonucleotide name Sequence (5¢- to 3¢) OMCA-KO-F OMCA-KO-R OMCB-KO-F OMCB-KO-R OMCA-F OMCA-R OMCB-F OMCB-R OMCA-PBAD-F CACACTGCAACCTCTGGT ACTGTCAATAGTGAAGGT ... acceptors U(VI) and Se(VI) is due tothe decaheme cytochromes c not recognizing either of these electron acceptors, we probed the relative affinities via competition assays Figure shows the IC50...